RESUMO
A system which utilizes a piston pump to generate cigarette smoke under standard conditions, and expose rodents to the inhalation of diluted smoke for controlled periods of time is described. Variations of the basic system have been employed to exposure groups of ten to twenty hamsters or rats, and should allow exposures of up to forty mice. The system has been in use for approximately 24 months in routine chronic exposures of rats. Data are presented to define the operating characteristics and typical dosimetry. Animal containment peculiar to this apparatus is described.
Assuntos
Equipamentos e Provisões , Fumar , Animais , Cricetinae , Ratos , PesquisaRESUMO
In order to avoid nasal absorption, an intralaryngeal cannula was tested as a device for bypassing the nose in delivering smoke into the lungs of hamsters. The intralaryngeal cannula increased lung deposition of radiolabeled smoke particles twofold to threefold over that in nose-breathing controls. Stabilization of respiration (absence of avoidance reaction), rather than prevention of nasal absorption of smoke, was demonstrated to be the cannula's major mechanism of action. Although suitable for acute exposures, the device is of limited use for chronic exposures that require daily insertion of the cannula during an extended period of time.
Assuntos
Modelos Animais de Doenças , Intubação Intratraqueal/métodos , Pulmão , Fumaça , Fumar , Animais , Cricetinae , Testes de Função Respiratória , Volume de Ventilação PulmonarRESUMO
An oral inhalation system for exposing rabbits to cigarette smoke has been developed. The essential components of the system comprise a smoke delivery device that adapts to the oral cavity, a molded face mask, and a sealable body enclosure. The functional capability of the system has been validated by detecting the presence of nicotine in plasma and by measuring the deposition of dotricontane labeled with radioactive carbon (14C) in lungs after exposure to cigarette smoke.
