Assuntos
Doenças dos Peixes/microbiologia , Tuberculose Pulmonar/veterinária , Animais , Peixes , Pulmão/microbiologia , Masculino , Infecções por Mycobacterium não Tuberculosas/microbiologia , Infecções por Mycobacterium não Tuberculosas/veterinária , Micobactérias não Tuberculosas/isolamento & purificação , Tuberculose Pulmonar/microbiologiaAssuntos
Ionização do Ar , Sangue , Campos Eletromagnéticos , Fenômenos Eletromagnéticos , Animais , Exposição Ambiental , Feminino , Camundongos , Fatores de TempoRESUMO
The caliciviruses, as a proposed family Caliciviridae, have a distinct virion morphology with cup-shaped depressions on a spherical capsid surface. The viruses have single-stranded RNA, which has a molecular weight about 2.6 x 10(6) and is infectious. The RNA is covalently linked to a small protein. A single major polypeptide is found in the capsid. A subgenomic RNA, molecular weight about 1 x 10(6), coding for the capsid polypeptide is found in infected cells. Caliciviruses infecting swine, pinnipeds and cats have been characterized. Viruses which are morphologically identical to the known caliciviruses have been identified in human feces; these viruses have been shown to be associated with gastroenteritis, but they have not yet been propagated in the laboratory.
Assuntos
Caliciviridae/classificação , Animais , Caliciviridae/genética , Caliciviridae/ultraestrutura , Humanos , Peso Molecular , RNA Viral/genéticaRESUMO
Three human melanoma cell lines derived from one primary and two metastatic tumors from three different patients were characterized for growth properties usually associated with malignant transformation; these include cell morphology, growth rate, saturation density, growth in semisolid media, colony-forming ability on contact-inhibited monolayers of normal fibroblasts and epithelial cells, and tumorigenicity in immunosuppressed mice. Variations in expression of aberrant properties were evident among the lines. One of the metastatic lines satisfied all the parameters of malignancy tested and the other showed a number of these properties, whereas the primary essentially fulfilled only one. These results suggest that cultured melanoma cells reflect the clinical variability often observed among melanoma patients and that metastatic melanoma seems to display a higher degree of malignant transformation than the primary.
Assuntos
Melanoma/patologia , Adulto , Animais , Divisão Celular , Linhagem Celular , Inibição de Contato , Feminino , Humanos , Cariotipagem , Masculino , Camundongos , Pessoa de Meia-Idade , Metástase Neoplásica , Transplante de NeoplasiasRESUMO
The levels of 13 components in the serum and eye fluids of the northern fur seal, Callorhinus ursinus, are compared. The wide variations observed would appear to limit the usefulness of eye fluid values as a substitute for serum values.
Assuntos
Líquidos Corporais/análise , Caniformia/metabolismo , Olho/metabolismo , Otárias/metabolismo , Animais , Otárias/sangue , MasculinoRESUMO
Analysis of 14 blood components in fresh and stored (107-166 days) frozen serum of the northern fur seal (Callorhinus ursinus) revealed significant changes in the values of all but total protein, globulins, phosphorus and creatinine. While most values decreased during storage, cholesterol and bilirubin showed small but significant increases.
Assuntos
Preservação de Sangue/veterinária , Caniformia/sangue , Otárias/sangue , Animais , Bilirrubina/sangue , Nitrogênio da Ureia Sanguínea , Colesterol/sangue , Feminino , Congelamento , MasculinoRESUMO
A virus was isolated from California sea lions (Zalophus californianus californianus) and northern fur seals (Callorhinus ursinus) in 1972. It was later named San Miguel sea lion virus (SMSV). State and federal livestock disease control agencies became concerned, because SMSV was found to be indistinguishable from vesicular exanthema of swine virus and to cause (in laboratory trials) clinical signs in swine similar to those produced by vesicular exanthema of swine virus. Ground carcasses of northern fur seals, salvaged after harvesting pelts, are fed to mink on ranches in the United States. Domestic swine are kept on some of these same ranches. Samples withheld from lots of this seal carcass mink food were found to contain SMSV (serotype 5) in titers of 10(6.1) and 10(6.8) tissue culture infective doses.
Assuntos
Ração Animal , Caniformia/microbiologia , Microbiologia de Alimentos , Picornaviridae/isolamento & purificação , Leões-Marinhos/microbiologia , Animais , Efeito Citopatogênico Viral , Picornaviridae/crescimento & desenvolvimentoRESUMO
Two new serotypes of San Miguel sea lion virus (SMSV), designated SMSV-4 and SMSV-5, were studied in vivo and in vitro. The host cell spectrums were compared with SMSV-1, SMSV-2, and vesicular exanthema of swine virus type A-48. Based on the result of these broad host spectrums, a numerical scoring system was devised for ranking each virus on the basis of its potential for infecting terrestrial mammals, including the important domestic species.
Assuntos
Picornaviridae/patogenicidade , Animais , Linhagem Celular , Cricetinae , Efeito Citopatogênico Viral , Cobaias , Picornaviridae/crescimento & desenvolvimento , Coelhos , Ratos , Suínos , Exantema Vesicular de Suínos/microbiologiaRESUMO
Clinical blood values are reported for 14 blood components in the northern fur seal (Callorhinus ursinus) and compared with human clinical ranges. Certain values such as cholesterol, globulins and blood urea nitrogen deviate markedly from published human values. The distribution of serum proteins in fur seals, man and northern elephant seals also are given and compared.
