RESUMO
Thyroid cell growth and function both in vivo and in vitro, are mainly regulated by TSH. Recent studies have shown that growth factors including insulin-like growth factors (IGF-1) have an important role in the control of thyrocyte proliferation and differentiation. The aim of this study was to evaluate the expression of the IGF-1 gene by Northern analysis and the IGF-1 tissue protein by radioimmunoassay in multinodular euthyroid goiters. The study population consisted of 20 patients with multinodular goiter (14 females and 6 males) living in a non endemic geographic area. All patients were euthyroid at the time of surgery and submitted to total or subtotal thyroidectomy. Samples of normal thyroid tissue were obtained from three patients who were operated due to laryngeal carcinomas. The IGF-1 protein content was increased in non toxic multinodular goiter, 22 ng/g vs. 14 in controls (p<0.03), as was IGF-1 gene expression (p<0.05). The increase in the steady state mRNA content correlated with the increase in the protein content (r=0.665; p<0.005). These results suggest that IGF-1 may play a role in proliferation events involved in benign hyperplastic thyroid diseases.
RESUMO
TTF1 mRNA expression in the thyroid gland from 30 patients with thyroid proliferating diseases and 4 healthy donors used as normal controls was investigated using Northern blot analysis. Six patients affected by malignant thyroid carcinomas and six patients with adenoma were examined; moreover 18 patients with non malignant proliferating disease such as multinodular goiters were investigated. Determination of TTF1 mRNA revealed a significant decrease of the steady state mRNA levels in carcinomas and adenomas as compared to normal tissues. On the contrary, in patients affected by multinodular goiters variations were observed that seemed not to be directly associated with the pathological conditions.
RESUMO
To find a clinical assay for histiocytosis X (HX) diagnosis, measurements were made of both activity and isoenzyme distribution of lactate dehydrogenase (LDH; EC 1.1.1.27) from the blood cells of 6 acute phase and 9 remission patients. A significant increase in the LDH activity measured in the monocytes and lymphocytes isolated from the blood of the acute phase patients was found. The increased activity was due to an enhancement of the normal pattern of LDH isoenzymes in these cells and not to a change in isoenzyme distribution. No increase was found in monocyte LDH isoenzymes from the patients in remission.
