RESUMO
In the present study, we isolated and identified a new fungus, Aspergillus sp VM-1 from banana pseudostem waste. The fungal strain, Aspergillus sp. VM-1 was grown on a substrate (banana pseudostem, cottonseed hulls and cottonseed meal in the ratio of 60:30:10, respectively) for 7 days and the crude enzyme was extracted from the fermented substrate. The pectinase activity in crude enzyme extract was 551 (U/ml). The crude enzyme extracted from Aspergillus sp. VM-1 was evaluated for bio-scouring of non-spinnable cotton. The non-spinnable cotton taken in the study was short staple cotton (SSC), cotton linters (CL) and non-woven cotton fabric (NWCF). The maximum absorbency (2 s) in non-spinnable cotton was achieved under optimized process conditions: enzyme extract level (40%), temperature (40 ± 2 °C) and time (40 min). The quality parameters of bio-scoured cotton meet the Indian Pharmacopoeia (IP) standards. A solid state fermenter was designed to scale-up the crude enzyme production up to 30 l. This is the first report on bio-scouring of under-utilized short cotton fibres at lower temperature (40 ± 2 °C). The present study offers a simple and eco-friendly bio-scouring process as an alternative to toxic chemical scouring of under-utilized short staple cotton fibres. Supplementary Information: The online version contains supplementary material available at 10.1007/s12649-021-01621-9.
RESUMO
An antibacterial metabolite extracted from Paenibacillus polymyxa HKA-15 showed strong inhibition against Xanthomonas campestris pv. phaseoli strains CP-1-1 and M-5. Minimum inhibitory concentration (MIC) of crude extract against strains CP-1-1 and M-5 was found to be 1.7 mg/ml and 1.52 mg/ml, respectively. In UV-Vis range, the absorption peak of crude extract was maximum at 240 nm. The compound is resilience to wide range of temperature, pH, surfactants and organic solvents. The complete loss of activity was observed when crude metabolite was treated with pepsin (400 unit/ml). Characterization of crude metabolite suggested its hydrophobic and peptide nature. Inhibition of Xanthomonas campestris pv. phaseoli by peptide like metabolite produced by Paenibacillus polymyxa strain HKA-15 under in vitro conditions showed ecological and biotechnological potential of strain HKA-15 to control common blight disease in beans.
