[Phenotyping of peripheral lymphocytes with monoclonal antibodies in newborn infants and adults]. / Fenotipizzazione linfocitaria periferica con anticorpi monoclonali nel neonato e nell'adulto.

The authors investigated and compared lymphocyte subsets natural killer in 33 newborns and in 33 healthy adults. From 5 ml of heparinized venous blood were separated mononuclear cells on density-gradient Ficoll-Hypaque. The vitality was determined with immunofluorescent method (orange acridine and ethiobromide). It was positive if resulted at least 95%. Lymphocyte phenotype was also examined after incubation with monoclonal antiserum Becton-Dickinson conjugated with FITC and PE for 45' at 4 degrees C. Two hundred cells were counted on each slide with fluorescent microscopy. Total number of T-lymphocyte (Leu-4) was increased in newborns; total B-cells (Leu-12) were similar in newborns and healthy adults; T-helper/T-suppressor (Leu-3a/Leu-2a) ratio was above the normal range for increase T-helper. The percentage of subsets with double marker Leu-2a+/Leu-3a+ was always about 15-20%. NK cells with phenotype HNK-1 (Leu-7) were a little number in newborns while the percentage of NK-cells-Leu-11a was similar among adults and newborns.

[Arterial pressure in childhood. I: Relation to age. (collection of data relative to 3,737 pupils of elementary and high school between the ages of 6 to 14 years)]. / La pressione arteriosa in età pediatrica. Parte I: Rapporto con l'età. (elaborazione di dati relativi a 3.737 alunni della Scuola elementare e media in età da 6 a 14 anni).

Research relating blood pressure levels of 6-14 years-old age schoolchildren has been carried into the whele school-population of Chivasso (Turin) town. Three kinds of armbands of different widths and lengths were used according to age. In conformity with Long's suggestions, the correction factors were used for the age-groups from 6 to 7 years and 8 to 11. On the grounds of the levels taken the quantiles were calculated. The graphs show the shape of the 5th, 25th, 50th, 75th, and 95th percentiles, related to the systolis and diastolic pressures and to the age of the child. The results showed a continous increase in the shape of the levels, which did not depend on the sex variant. A spike in the 9th year can be related to the fact that the correction factor can be less than it should be. The decrease in the 11th year can be put down to the fact that the correction for the armband has not been carried out from the 12th year onwards. 6.52% of schoolchildren have systolic and/or diastolic blood pressure greater than 95 degrees C.