Wicking assisted condenser platform with patterned wettability for space application.

Vapor condensation is extensively used in applications that demand the exchange of a substantial amount of heat energy or the vapor-liquid phase conversion. In conventional condensers, the condensate removal from a subcooled surface is caused by gravity force. This restricts the use of such condensers in space applications or horizontal orientations. The current study demonstrates proof-of-concept of a novel plate-type condenser platform for passively removing condensate from a horizontally oriented surface to the surrounding wicking reservoir without gravity. The condensing surface is engineered with patterned wettabilities, which enables the continuous migration of condensate from the inner region of the condenser surface to the side edges via surface energy gradient. The surrounding wicking reservoir facilitates the continuous absorption of condensate from the side edges. The condensation dynamics on different substrates with patterned wettabilities are investigated, and their condensation heat transfer performance is compared. The continuous migration of condensate drops from a superhydrophobic to a superhydrophilic area can rejuvenate the nucleation sites in the superhydrophobic area, resulting in increased heat transport. The proposed condenser design with engineered wettability can be used for temperature and humidity management applications in space.

Formation of Two-Dimensional Magnetically Responsive Clusters Using Hematite Particles Self-Assembled via Particle-Induced Heating at an Interface.

Hematite particles, which exhibit a high magnetic moment, are used to apply large forces on physical and biological systems under magnetic fields to investigate various phenomena, such as those of rheology and micromanipulation. However, the magnetic confinement of these particles requires complicated field configurations. On the other hand, laser-assisted optical confinement of single hematite particles results in thermophoresis and subsequent ejection of the particle from the laser spot. Herein, we explore an alternative strategy to induce the self-assembly of hematite. In this strategy, with indirect influence from an optically confined and heated upconverting particle (UCP) at an air-water interface, there is the generation of convection currents that facilitate assembly. We also show that the assembly remains at the interface even after removal of the laser light. The hematite particle assemblies can then be moved using magnetic fields and employed to perform interfacial rheology.

Massively Parallel High-Throughput Single-Cell Patterning and Large Biomolecular Delivery in Mammalian Cells Using Light Pulses.

The recent advancements of single-cell analysis have significantly enhanced the ability to understand cellular physiology when compared to bulk cellular analysis. Here a massively parallel single-cell patterning and very large biomolecular delivery is reported. Micro-pillar polydimethyl siloxane stamp with different diameters (40-100 µm with 1 cm × 1 cm patterning area) is fabricated and then imprint distinct proteins and finally pattern single-cell to small clusters of cells depending on the micro-pillar diameters. The maximum patterning efficiency is achieved 99.7% for SiHa, 96.75% for L929, and 98.6% for MG63 cells, for the 100 µm micro-pillar stamp. For intracellular delivery of biomolecules into the patterned cells, a titanium micro-dish device is aligned on top of the cells and exposed by infrared light pulses. The platform successfully delivers small to very large biomolecules such as PI dyes (668 Da), dextran 3000 Da, siRNA (20-24 bp), and large size enzymes (464 KDa) in SiHa, L929 and MG63 cells. The delivery efficiency for PI dye, Dextran 3000, siRNA, and enzyme for patterned cells are ≈95 ± 3%, 97 ± 1%, 96 ± 1% and 94 ± 3%, with cell viability of 98 ± 1%. Thus, the platform is compact, robust, easy for printing, and potentially applicable for single-cell therapy and diagnostics.

3D Paper-based milk adulteration detection device.

Milk adulteration is a common problem in developing countries, and it can lead to fatal diseases in humans. Despite several studies to identify different adulterants in milk samples, the effects of multiple adulterants remain unexplored. In this work, a three-dimensional (3D) paper-based microfluidic device is designed and fabricated to simultaneously detect multiple chemical adulterants in milk. This device comprises a top cover, a bottom cover, and a middle layer composed of transportation and a detection zone. By making cuts on the middle layer's support, the device's flow path is characterised by optimum and uniform velocity. For the first time, seven adulterants (urea, detergents, soap, starch, hydrogen peroxide, sodium-hydrogen-carbonate, and salt) are detected in the milk sample simultaneously with specificity evaluation and detailed color interference analysis. Only 1-2 mL of sample volume is required to detect 7 adulterants at one time. We have used only 10 [Formula: see text]L of the reagent's volume for the colorimetric reaction and found the results within a few seconds. Observation reveals that the limit of detection (LOD) of the adulterants lies in the range between [Formula: see text] (vol./vol.) to [Formula: see text] (vol./vol.) using the colorimetric detection technique. The unknown quantity of the added adulterants is measured using the calibration curves obtained from the experiments results. The repeatability and reproducibility of the process, sensitivity, and the linear range of detection of the calibration curves and the statistical study of the color intensity data are thoroughly analysed herein. In any resource-limited setting, this simple, portable, and user-friendly 3D microfluidic device is expected to be used for testing liquid foods before consumption.

Facets of optically and magnetically induced heating in ferromagnetically doped-NaYF4 particles.

Upconverting particles like Yb and Er-doped NaYF4 are known to heat up after illumination with light at pump wavelength due to inefficient upconversion processes. Here we show that NaYF4 particles which have been co-doped not only with Yb and Er but also Fe improves the photothermal conversion efficiency. In addition, we show for the first time that alternating magnetic fields also heat up the ferromagnetic particles. Thereafter we show that a combination of optical and magnetic stimuli significantly increases the heat generated by the particles.

Alignment-mediated segregation in an active-passive mixture.

We report segregation between the athermal active and passive particles mediated by the local alignment interaction in a confined space. The competition between the alignment interaction and self-propulsion force results in a transition between disordered and ordered phases. We show that as the coordination between the particles increases, they form an ordered mill, which helps the particles to aggregate into isotropic clusters. As a result, particles segregate into active core and passive shells. This segregation phenomenon is adversely affected by the packing fraction and the size dispersion between active and passive particles. We show that this adverse effect can be overcome by incorporating higher coordination in the system. We report that the monodispersed system is more desirable for segregation in a binary mixture than a bidispersed system, as the latter favors the mixed state.

Pulsed laser assisted high-throughput intracellular delivery in hanging drop based three dimensional cancer spheroids.

Targeted intracellular delivery of biomolecules and therapeutic cargo enables the controlled manipulation of cellular processes. Laser-based optoporation has emerged as a versatile, non-invasive technique that employs light-based transient physical disruption of the cell membrane and achieves high transfection efficiency with low cell damage. Testing of the delivery efficiency of optoporation-based techniques has been conducted on single cells in monolayers, but its applicability in three-dimensional (3D) cell clusters/spheroids has not been explored. Cancer cells grown as 3D tumor spheroids are widely used in anti-cancer drug screening and can be potentially employed for testing delivery efficiency. Towards this goal, we demonstrated the optoporation-based high-throughput intracellular delivery of a model fluorescent cargo (propidium iodide, PI) within 3D SiHa human cervical cancer spheroids. To enable this technique, nano-spiked core-shell gold-coated polystyrene nanoparticles (ns-AuNPs) with a high surface-to-volume ratio were fabricated. ns-AuNPs exhibited high electric field enhancement and highly localized heating at an excitation wavelength of 680 nm. ns-AuNPs were co-incubated with cancer cells within hanging droplets to enable the rapid aggregation and assembly of spheroids. Nanosecond pulsed-laser excitation at the optimized values of laser fluence (45 mJ cm-2), pulse frequency (10 Hz), laser exposure time (30 s), and ns-AuNP concentration (5 × 1010 particles per ml) resulted in the successful delivery of PI dye into cancer cells. This technique ensured high delivery efficiency (89.6 ± 2.8%) while maintaining high cellular viability (97.4 ± 0.4%), thereby validating the applicability of this technique for intracellular delivery. The optoporation-based strategy can enable high-throughput single cell manipulation, is scalable towards larger 3D tissue constructs, and may provide translational benefits for the delivery of anti-cancer therapeutics to tumors.

Fabrication of TiO2 microspikes for highly efficient intracellular delivery by pulse laser-assisted photoporation.

The introduction of foreign cargo into living cells with high delivery efficiency and cell viability is a challenge in cell biology and biomedical research. Here, we demonstrate a nanosecond pulse laser-activated photoporation for highly efficient intracellular delivery using titanium dioxide (TiO2) microspikes as a substratum. The TiO2 microspikes were formed on titanium (Ti) substrate using an electrochemical anodization process. Cells were cultured on top of the TiO2 microspikes as a monolayer, and the biomolecule was added. Due to pulse laser exposure of the TiO2 microspike-cell membrane interface, the microspikes heat up and induce cavitation bubbles, which rapidly grow, coalesce and collapse to induce explosion, resulting in very strong fluid flow at the cell membrane surface. Thus, the cell plasma membrane disrupts and creates transient nanopores, allowing delivery of biomolecules into cells by a simple diffusion process. By this technique, we successfully delivered propidium iodide (PI) dye in HeLa cells with high delivery efficiency (93%) and high cell viability (98%) using 7 mJ pulse energy at 650 nm wavelength. Thus, our TiO2 microspike-based platform is compact, easy to use, and potentially applicable for therapeutic and diagnostic purposes.

Spatiotemporal dynamics of a self-propelled system with opposing alignment and repulsive forces.

Effect of concurrent alignment and repulsion is studied in the purview of a confined active matter system using a modified force-based Vicsek model. On alteration of the alignment and the repulsive force parameters, a low alignment random phase, a midrange alignment milling phase, and a high alignment oscillatory phase are identified. Based on the particle aggregations, the milling phase is further classified into three subphases, two of which are spatial patterns: one consisting of compact ring-shaped mills and the other incorporating both rings and clusters. A correlation function based on the inner product of spatial velocity fluctuations of the particles shows a high correlation length for the ringed milling and the rings-clusters hybrid milling state. On analyzing temporal velocity fluctuations of particles through chaos detection techniques, low alignment and high alignment states are indicative of chaos, while the middle order alignment is symbolic of periodicity. The extent of synchronization of the particles' motion is analyzed through a Hilbert transform-based mean frequency approach, leading to the detection of a weak chimera state in the case of the spatial structures. The ringed milling state shows a unique category of weak chimera consisting of multiple oscillator groups showcasing different synchronization frequencies coexisting with desynchronized oscillators.

Liquid Wicking in a Paper Strip: An Experimental and Numerical Study.

In this decade, paper-based microfluidics has gained more interest in the research due to the vast applications in medical diagnosis, environmental monitoring, food safety analysis, etc. In this work, we presented a set of experiments to understand the physics of the capillary flow phenomenon through paper strips. Here, using the wicking phenomenon of the liquid in porous media, experimentally, we find out the capillary height of the liquid in filter paper at different time intervals. It was found that the Lucas-Washburn (L-W) model, as well as the evaporation model, fails to predict the capillary rise accurately. However, the detailed numerical solution shows a better similarity with the experimental results. We have also shown the different regimes of the wicking phenomenon using scaling analysis of the modified L-W model. The capillary rise method was applied to detect the added water content in milk. We used milk as a liquid food and found the added water content from the change in the capillary height at different concentrations of milk. Finally, results obtained from the paper-based device were verified with the commercially available lactometer data.

Effect of particle fraction on phase transitions in an active-passive particles system.

We study phase transition in a binary system of monodisperse active and passive particles. The particles are initially randomly positioned inside a fixed boundary square enclosure. The active particles can move with their self-propulsion force. Whereas, the passive particles do not have any self-propulsion force, and they move by the spatial interactions with other particles. An alignment force in our discrete element model causes the emergence of collective milling motion. Without this alignment interaction, the particle system remains in a disordered phase. Whereas, the ordered milling phase is attained after achieving a minimum coordination among neighboring particles. The phase transition from disordered to ordered depends upon the relative effect of self-propulsion and the alignment, initial states of the particles, noise level, and the fraction of the active particles present in the system. The phase transition we observed is of first-order nature.

Surface Treatments to Enhance the Functionality of PPEs.

The outbreak of unknown viral pneumonia in Wuhan China in December 2019 led to a new coronavirus (SARS-CoV-2), which attracted worldwide attention, with the related COVID-19 disease quickly becoming a global pandemic. In about 5 months, this disease has led to ~ 4 million cases and claimed more than 200 k deaths as a result of its highly contagious nature. The present understanding is that SARS-CoV-2 is a type of influenza virus that can be transmitted through respiratory droplets and aerosols; Lewis (Nature 580:175, 2020). The primary methodology to prevent the spreading of this disease has been "social distancing" and usage of personal protective equipment (PPE) at the front lines of healthcare and other critical operations. The scale of the disease has led to unprecedented demand for PPEs and increased functionality of the same. This paper focuses on improving PPE functionality in a scalable manner by surface treatment and coating with appropriate materials and other functional enhancements, such as exposure to UV rays or other sterilizing agents (e.g., hydrogen peroxide).

Confined System Analysis of a Predator-Prey Minimalistic Model.

In nature exists a properly defined food chain- an order of hunting and getting hunted. One such hunter-hunted pair is considered in this context and coordinated escape manoeuvres in response to predation is studied in case of a rarely examined confined system. Both the predator agent and prey agents are considered to be self-propelled particles moving in a viscous fluid. The state of motility when alive and passivity on death has been accounted for. A novel individual-based combination of Vicsek model and Boids flocking model is used for defining the self-propelling action and inter-agent interactions. The regimes observed at differing levels of co-ordination segregated by quantification of global order parameter are found to be in agreement with the extant literature. This study strives to understand the penalty on the collective motion due to the restraints employed by the rigid walls of the confinement and the predator's hunting tactics. The success of any escape manoeuvre is dependent on the rate of information transfer and the strength of the agitation at the source of the manoeuvre. The rate of information transfer is studied as a function of co-ordination and the size of the influence zone and the source strength is studied as a function of escape acceleration activated on the agitated prey. The role of these factors in affecting survival rate of prey is given due coverage.

Activity-induced mixing and phase transitions of self-propelled swimmers.

We study the mixing of active swimmers. Two different types of swimmers (modeled as particles) are placed initially in two boxes with an interconnection between them. The mixing of swimmers happens as they move with their own self-propelled forces. The self-propelled force is constant and the direction of the exerted thrust is governed by the neighboring swimmers. Overall mixing of the swimmers depends on the magnitude of the exerted thrust, the initial packing fraction, and the activity level. Different nonequilibrium states are also identified depending on the exerted thrust and the initial packing fraction of the swimmers.

Transitions between multiple dynamical states in a confined dense active-particle system.

We study the collective motion of a dense suspension of active swimmers in a viscous fluid medium. The swimmers are modeled as soft spheres moving in a highly viscous fluid medium. The magnitude of the propelling thrust exerted by each particle is taken to be a constant and the direction is aligned to its velocity. Depending on the magnitude of the exerted thrust, several nonequilibrium steady states are observed. The transitions between the steady states are characterized using the total dissipation as a function of the magnitude of the thrust. The transitions between the nonequilibrium states are characterized by changes in exponent at low thrust values. At high thrust values, hysteretic transitions between ordered and disordered states are observed.

Rapid, Self-driven Liquid Mixing on Open-Surface Microfluidic Platforms.

Self-driven surface micromixers (SDSM) relying on patterned-wettability technology provide an elegant solution for low-cost, point-of-care (POC) devices and lab-on-a-chip (LOC) applications. We present a SDSM fabricated by strategically patterning three wettable wedge-shaped tracks onto a non-wettable, flat surface. This SDSM operates by harnessing the wettability contrast and the geometry of the patterns to promote mixing of small liquid volumes (µL droplets) through a combination of coalescence and Laplace pressure-driven flow. Liquid droplets dispensed on two juxtaposed branches are transported to a coalescence station, where they merge after the accumulated volumes exceed a threshold. Further mixing occurs during capillary-driven, advective transport of the combined liquid over the third wettable track. Planar, non-wettable "islands" of different shapes are also laid on this third track to alter the flow in such a way that mixing is augmented. Several SDSM designs, each with a unique combination of island shapes and positions, are tested, providing a greater understanding of the different mixing regimes on these surfaces. The study offers design insights for developing low-cost surface microfluidic mixing devices on open substrates.