Assuntos
Leucemia Mielomonocítica Aguda/diagnóstico , Monócitos/enzimologia , Peroxidase/deficiência , Adulto , Idoso , Antineoplásicos/uso terapêutico , Contagem de Eritrócitos , Feminino , Humanos , Leucemia Mielomonocítica Aguda/sangue , Leucemia Mielomonocítica Aguda/tratamento farmacológico , Contagem de Leucócitos , Masculino , Peroxidase/sangueRESUMO
Healthy reference ranges for the total peripheral reticulocyte count and its three subpopulations, LFR (low fluorescence ratio), MFR (middle fluorescence ratio) and HFR (high fluorescence ratio) were established on 1219 healthy subjects. Samples were taken from subjects of both sexes with ages ranging from 4 to over 60 years. The observed ranges were found to be different between males and females for the group over 20 years old, while there was no difference between sexes found at the age between 4 and 19 years.
Assuntos
Reticulócitos/citologia , Adolescente , Adulto , Benzofenoneídio , Preservação de Sangue , Criança , Pré-Escolar , Feminino , Citometria de Fluxo/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Contagem de Reticulócitos/métodos , Fatores Sexuais , Temperatura , Fatores de TempoRESUMO
The reticulocyte count is a good index of erythropoietic activity. Automated methods, mainly flow cytometry, have made the counting easier, more accurate and reproducible. The data presented describe the counting of reticulocytes in 1219 apparently healthy subjects of both sexes who came for a periodic health examination. Their ages varied from 4 to over 60 years. The reticulocytes were counted with a flow cytometer (Sysmex R-3000) with Auramine-O as fluorochrome. There was no statistical difference between boys and girls aged 4-19 years. However, the reticulocyte count was significantly higher in men than in women over 20 years of age. The reticulocyte count was affected neither by the menstrual cycle in girls at puberty, nor by oral contraceptives and the menopause in women. No effect of moderate smoking was observed in either sex. Reference limits of the reticulocyte counts (percentage and absolute value) according to age and sex and the three types of reticulocytes are provided.
Assuntos
Contagem de Eritrócitos , Reticulócitos/citologia , Adolescente , Adulto , Envelhecimento/sangue , Criança , Pré-Escolar , Feminino , Citometria de Fluxo , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Caracteres Sexuais , Fumar/sangueRESUMO
The HSV (1 or 2) is the cause of serious pulmonary infections among patients who have had a transplantation. This study in retrospect is based on the analysis of 145 patients who underwent a cardiothoracic transplant at the CHU. in Nancy. Confronted with clinical signs calling to mind breathing difficulties, the analysis of the broncho alveolar lavage (or of the bronchial brushing) revealed the viral aetiological agent. The answer from the laboratory is quickly available by immunofluorescence or by immunoperoxidase with viral anti-protein monoclonal antibodies and by the multiplication in vitro of the virus into cell cultures. The HSV 1 was responsible for 8 herpetic lung infections. The specific Acyclovir treatment was used 6 times successfully. When such a direction of treatment was impossible (in 2 cases) the outcome was fatal. The carry HSV is highly frequent and recurrences under immuno-suppressor treatment require an Acyclovir prophylaxis among patients admittedly carrying the virus in a pre-transplanted serum assessment.