Pickering Emulsions Stabilized by Chitosan/Natural Acacia Gum Biopolymers: Effects of pH and Salt Concentrations.

In this study, chitosan (CT) and naturally occurring acacia gum (AG) blends were employed as emulsifiers to form a series of emulsions developed from diesel and water. Effects of pH level (3, 5, 10, and 12) and various NaCl salt concentrations (0.25-1%) on the stability, viscosity, and interfacial properties of CT-(1%)/AG-(4%) stabilized Pickering emulsions were evaluated. Bottle test experiment results showed that the stability indexes of the CT/AG emulsions were similar under acidic (3 and 5) and alkaline (10 and 12) pH media. On the other hand, the effects of various NaCl concentrations on the stability of CT-(1%)/AG-(4%) emulsion demonstrated analogous behavior throughout. From all the NaCl concentrations and pH levels examined, viscosities of this emulsion decreased drastically with the increasing shear rate, indicating pseudoplastic fluid with shear thinning characteristics of these emulsions. The viscosity of CT-(1%)/AG-(4%) emulsion increased at a low shear rate and decreased with an increasing shear rate. The presence of NaCl salt and pH change in CT/AG solutions induced a transformation in the interfacial tension (IFT) at the diesel/water interface. Accordingly, the IFT values of diesel/water in the absence of NaCl/CT/AG (without emulsifier and salt) remained fairly constant for a period of 500 s, and its average IFT value was 26.16 mN/m. In the absence of salt, the addition of an emulsifier (CT-(1%)/AG-(4%)) reduced the IFT to 16.69 mN/m. When the salt was added, the IFT values were further reduced to 12.04 mN/m. At low pH, the IFT was higher (17.1 mN/M) compared to the value of the IFT (10.8 mN/M) at high pH. The results obtained will help understand the preparation and performance of such emulsions under different conditions especially relevant to oil field applications.

Fluorinated surfactants: A review on recent progress on synthesis and oilfield applications.

The selection of appropriate chemicals and the synthetic method plays an important role in oilfield application. The objectives of this study are to describe the various synthetic route for the preparation of fluorinated surfactants and highlight their oilfield applications. Fluorinated surfactants are the type of surfactants where the hydrophobic tail is either partially fluorinated or replaced totally with fluorine molecules. Fluorinated surfactants have distinct properties compared to corresponding hydrocarbon surfactants such as lower surface tension, better efficiency in lowering the interfacial tension, both oleophobic and hydrophobic nature, high thermal stability, and better chemical tolerance. These properties make them a material of choice for several applications which include but are not limited to fire-fighting, household items, foaming, coating, and paints. Despite these attractive properties, environmental concerns associated with fluorinated surfactants is a major hurdle in extending the application of such surfactants. This review discusses the various synthetic routes for the synthesis of different classes of surfactants such as cationic, anionic, non-ionic, and zwitterionic surfactants. The fundamental surface/interface properties of the synthesized surfactants are also highlighted. In addition, the review highlights the application of fluorinated surfactants in the oil & gas industry.

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics.

Multiple Ovulation and Embryo Transfer (MOET) technology is a potential technique to upgrade livestock species' genetics. The varied response to super-stimulatory treatments remains one of the limiting factors to this technology's widespread use. The present study was aimed to improve the superovulation response and in-vivo embryo production by using controlled internal drug release (CIDR)-GnRH or CIDR-EB (Estradiol Benzoate) along with conventional superovulation protocol in Holstein Frisian (HF): Bos taurus; n = 42) and Crossbred (XB: Cholistani (Bos indicus) × HF; n = 28) cows. In the CIDR-GnRH/CIDR-EB treatment, CIDR was implanted in the cows after confirming the presence of a corpus luteum (CL) on the 8th day after estrus. 2 ml GnRH (Lecirelin acetate 0.0262 mg/ml) or 2 mg EB was also administered in CIDR-GnRH/CIDR-EB groups, respectively. Both groups were given super-stimulatory treatment from the 11th day after estrus (FSH in tapering doses twice a day for four consecutive days). On day 13, two doses of 2 ml prostaglandin (75 µg/ml of dextrorotatory cloprostenol) were administered (am: pm), and CIDR was removed the following day. Two artificial inseminations (AI) of the cows were performed (12 h apart) on the 15th day. No CIDR and GnRH/E.B were given in the control group, but the remaining superovulation protocol was the same. Later on, seven days after the first AI, non-surgical embryo flushing was done. The transferable embryos produced from three different superovulation protocols were then transferred into the recipient cows (n = 90) for determining their fertility. Statistical analysis revealed that the number of super-estrus follicles (SEF), multiple corpora lutea (MCL), ovulation/fertilization percentage, fertilized structures recovered (FSR), and transferable embryos (TEs) remained significantly higher (p < 0.05), and days taken for return to estrus (RTE) after embryo collection remained significantly lower (p < 0.05) in CIDR-GnRH (n = 18) and CIDR-EB (n = 15) groups as compared to the control (n = 37). The comparison between XB and HF cows revealed that the TEs production in CIDR-GnRH (XB = 5 vs HF = 13) and CIDR-EB (XB = 6 vs HF = 9) based superovulation protocols were 11.60  ±  4.08 vs 04.31  ±  0.98 and 09.33  ±  1.78 vs 05.22  ±  1.36, respectively. TEs production in XB cows (n = 5) of the CIDR-GnRH group was significantly higher (11.60  ±  4.08) than other groups. On the other hand, the days taken for RTE after embryo collection remained significantly lower (p < 0.05) in HF cows of treatment groups. However, the fertility of TEs was neither affected significantly (p > 0.05) by the superovulation protocol used nor by breed differences among donor cows. In conclusion, using CIDR-GnRH or CIDR-EB along with conventional superovulation protocol may enhance the efficiency of MOET programs in cattle. Furthermore, XB donor cows demonstrated a better performance than HF donor cows under subtropical conditions.

Magnetic surfactants: A review of recent progress in synthesis and applications.

Magnetic surfactants are a special class of surfactants with magneto-responsive properties. These surfactants possess lower critical micelle concentrations and are more effective in reducing surface tension as compared to conventional surfactants. Such surfactants' ability to manipulate self-assembly in a controlled way by tuning the magnetic field makes them an attractive choice for several applications, including drug delivery, catalysis, separation, oilfield, and water treatment. In this work, we reviewed the properties of magnetic surfactants and possible explanations of magnetic behavior. This article also covers the synthesis methods that can be used to synthesize different types of cationic, anionic, nonionic, and zwitterionic magnetic surfactants. The applications of magnetic surfactants in different fields such as biotechnology, water treatment, catalysis, and oilfield have been discussed in detail.

Synthesis and Evaluation of Zwitterionic Surfactants Bearing Benzene Ring in the Hydrophobic Tail.

Surfactant tolerance in the presence of mono and divalent reservoir ions, as well as the solubility of surfactant in high salinity and low salinity brine, are the two major requirements for any surfactant that is subjected to oilfield application. Herein, six poly(ethylene oxide) zwitterionic surfactants having different ionic headgroups and hydrophobic tail were synthesized for oilfield applications. They were characterized by various instrumental techniques (Fourier-transform infrared spectroscopy (FTIR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS), Nuclear Magnetic Resonance (NMR)) and the combination of these techniques allowed for us to deduce the structure. All of the surfactants revealed prominent solubility in high salinity and low salinity brine due to the presence of ethoxy units between the aromatic ring and amide group. The surfactant samples were oven aged for 90 days at reservoir temperature and a clear solution implies their excellent aqueous stability. Rendering to thermal gravimetric results, decomposition of surfactants was found to occur around 300 °C, which is higher than the reservoir temperature (≥90 °C). It was observed that the hydrophilic headgroup has no significant impact on the critical micelle concentration and other surface properties. However, the hydrophobic tail bearing benzene ring significantly alters the critical micelle concentration and other surface properties.

Poly(Oxyethylene)-Amidoamine Based Gemini Cationic Surfactants for Oilfield Applications: Effect of Hydrophilicity of Spacer Group.

Thermal stability, salt tolerance, and solubility in normal and high salinity brine are the major requirements for any surfactant designed for oilfield applications because the surfactant stays in a non-ambient environment inside the reservoir for a long period of time. Herein, a series of new gemini cationic surfactants (GSs) with varying spacer hydrophilicity were synthesized and elucidated using MALDI-ToF-MS, NMR (1H, 13C), as well as FTIR spectroscopy. GSs found to be soluble in normal as well as high salinity brine and aqueous stability tests revealed that GSs possess the ability to retain their structural integrity at high salinity and high temperature conditions because no suspension formation or precipitation was detected in the oven aged sample of GSs at 90 °C for 30 days. Thermal gravimetric analysis displayed a higher decomposition temperature than the real reservoir temperature and the GS with a secondary amine spacer exhibited high heat stability. The significant reduction in surface tension and critical micelle concentration was observed using 1 M NaCl solution in place of deionized water. The difference in surface tension and critical micelle concentration was insignificant when the 1 M NaCl solution was replaced with seawater. The synthesized surfactants can be utilized for oilfield applications in a challenging high temperature high salinity environment.

Purification and characterization of thermostable chitinase from a novel S. maltophilia strain

Aims: The presents study examines the purification and characterization of a chitinase from S. maltophilia SJ602 strain isolated from a soil sample collected from Jamia Hamdard, New Delhi. Methodology and Results: The purification steps included chitin affinity using colloidal chitin as the affinity matrix and column chromatography using Sephadex G-100. The chitinase was purified to 66 fold having a yield of 17%. The molecular weight of the chitinase was found to be around 29 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The pH and temperature optima of the purified chitinase were found to be at pH 5.5 and 60 °C, respectively. Conclusion, Significance and Impact of the study: Besides showing a significant yield, the enzyme has a high thermal stability which has its applicability in the recycling of chitin waste.