Physiological specialization of Puccinia triticina and genome-wide association mapping provide insights into the genetics of wheat leaf rust resistance in Iran.

Leaf rust caused by Puccinia triticina Erikss. (Pt) is the most widely distributed and important wheat disease worldwide. The objective of the present study was to determine the frequency of Iranian Pt races, their virulence to key resistance genes and map quantitative trait loci (QTL) for resistance to different Pt races from 185 globally diverse wheat genotypes using a genome-wide association study (GWAS) approach. The virulence pattern of the 33 Pt isolates from various wheat-growing areas of Iran on 55 wheat differentials showed that the FKTPS and FKTTS were relatively frequent pathotypes among the 18 identified races. The weighted average frequency of virulence on the resistance genes Lrb, Lr3bg, Lr14b, Lr16, Lr24, Lr3ka, Lr11 and Lr20 were high (> 90%). However, low virulence on the resistant genes Lr2a, Lr9, Lr19, Lr25, Lr28 and Lr29 indicates that these genes are still effective against the pathogen population in Iran at present. GWAS on a panel of 185 wheat genotypes against 10 Pt races resulted into 62 significant marker-trait associations (MTAs) belonged to 34 quantitative trait loci (QTL) across 16 chromosomes. Among them, 10 QTLs on chromosomes 1A, 1B, 3B, 3D, 4A, 6D, 7A and 7D were identified as potential novel QTLs, of which four QTLs (QLr.iau-3B-2, QLr.iau-7A-2, QLr.iau-7A-3 and QLr.iau-7D-2) are more interesting, as they are associated with resistance to two or more Pt races. The known and novel QTLs associated with different Pt races found here, can be used in future wheat breeding programs to recombine different loci for durable resistance against leaf rust races.

Genetic analysis of novel resistance sources and genome-wide association mapping identified novel QTLs for resistance to Zymoseptoria tritici, the causal agent of septoria tritici blotch in wheat.

Septoria tritici blotch (STB) caused by Zymoseptoria tritici is one of the most important foliar diseases of wheat causing significant yield losses worldwide. In this study, a panel of bread wheat genotypes comprised 185 globally diverse genotypes were tested against 10 Z. tritici isolates at the seedling stage. Genome-wide association study (GWAS) using high-throughput DArTseq markers was performed and further gene expression analysis of significant markers trait association (MTAs) associated with resistance to STB was analyzed. Disease severity level showed significant differences among wheat genotypes for resistance to different Z. tritici isolates. We found novel landrace genotypes that showed highly resistance spectra to all tested isolates. GWAS analysis resulted in 19 quantitative trait loci (QTLs) for resistance to STB that were located on 14 chromosomes. Overall, 14 QTLs were overlapped with previously known QTLs or resistance genes, as well as five potentially novel QTLs on chromosomes 1A, 4A, 5B, 5D, and 6D. Identified novel resistance sources and also novel QTLs for resistance to different Z. tritici isolates can be used for gene pyramiding and development of durable resistance cultivars in future wheat breeding programs.