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1.
Environ Technol ; 44(8): 1156-1168, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34704540

RESUMO

In this work, g-C3N4/ZIF-8 heterojunction photocatalysts were synthesised by the process by which the metal-organic framework ZIF-8 nanoparticles were grown onto the g-C3N4 layer in situ. Bismuth element was doped into the as-prepared g-C3N4/ZIF-8 material and a new type of Bi@g-C3N4/ZIF-8 composite photocatalysts was manufactured, in which the doping element acts in adjusting the bandgap in the photocatalysts. The prepared photocatalysts were characterised by XRD, FESEM, TEM, FTIR, XPS, UV-VIS DRS, photoluminescence and photo-electrochemical experiments. The results show that the ZIF-8 nanoparticles grown in situ were well-formed onto the g-C3N4 layer, and bismuth was evenly doped into the gaps of the g-C3N4/ZIF-8 framework. The degradation rate of methylene blue by CNZ-1.5(Bi)-12, which was a photocatalyst composed of 12% Bi-doped with g-C3N4/ZIF-8 material (the mass ratio of g-C3N4: ZIF-8 = 1:1.5), reached 86.6% under visible light irradiation within 60 min. The free radical scavenging experiment and electron spin resonance spectroscopy showed that ∙OH was the main active substance. Bismuth doping into the photocatalytic system promotes the excitation of electrons from the valence band to the conduction band and provides a good channel for the transmission of photogenerated carriers as well. It is achieved that intensive visible light absorption, the enhanced separation efficiency of photogenerated carriers, and excellent thermal stability and high recyclability in the novel composite photocatalyst, owing to the synergistic effect of the introduced bismuth with the heterostructure of g-C3N4/ZIF-8. Therefore, the synthesised Bi@g-C3N4/ZIF-8 heterojunction photocatalysts may be used as a good photocatalyst for purifying and degrading organic matter in sewage.


Assuntos
Grafite , Iluminação , Bismuto/química , Grafite/química , Catálise , Luz
2.
Int J Biol Macromol ; 225: 27-39, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36495771

RESUMO

Stimulator of interferon genes (STING) plays important roles in innate immunology. In this study, we isolated the STING gene in Nile tilapia, termed OnSTING. Using quantitative RT-PCR, we explored the expression patterns of the OnSTING gene. Using dual-luciferase reporter assays, we revealed the effect of STING overexpression on nuclear factor κB (NF-κB), IFN and AP activation in HEK 293 cells. Using coimmunoprecipitation, the interaction of STING and TRIF was studied. The effect of OnSTING overexpression on the antibacterial activity in tilapia was investigated. The results showed that upon stimulation with Streptococcus agalactiae, the OnSTING transcript was upregulated in all the tested tissues. OnSTING mRNA levels were very stable from 2.5 to 8.5 dpf. Moreover, OnSTING, OnIFN and IRF3 expression was induced by LPS, Poly (I:C), S. agalactiae WC1535 and DCPS in Nile tilapia macrophages. Overexpression of OnSTING and OnDDX41 increased NF-κB activation in HEK293T cells and slightly increased IFN-ß activation but had no effect on AP-1 activation. OnSTING interacted with OnDDX41 and OnTBK1. However, OnSTING did not interact with TRIF. OnSTING overexpression in vivo decreased the sensitivity of tilapia to S. agalactiae infection. These results are helpful for clarifying the innate immune response against bacterial infection in Nile tilapia.


Assuntos
Ciclídeos , Doenças dos Peixes , Tilápia , Animais , Humanos , Ciclídeos/genética , Ciclídeos/metabolismo , NF-kappa B/metabolismo , Células HEK293 , Fator de Transcrição AP-1/genética , Fator de Transcrição AP-1/metabolismo , Transdução de Sinais , Imunidade Inata/genética , Tilápia/genética , Tilápia/metabolismo , DNA/metabolismo , RNA Helicases/metabolismo , Proteínas Adaptadoras de Transporte Vesicular/genética , Streptococcus agalactiae/genética , Proteínas de Peixes/metabolismo , Regulação da Expressão Gênica
4.
J Fish Biol ; 101(1): 144-153, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35514248

RESUMO

Tripartite motif (TRIM) proteins play a regulatory function in cancer, cell apoptosis and innate immunity. To understand the role of TRIM39 in Nile tilapia (Oreochromis niloticus), TRIM39 cDNA was isolated. The total length of TRIM39 cDNA was 5025 bp. The deduced OnTRIM39 protein contains 549 amino acids and has conserved domains of the TRIM family, which are the RING, B-box, coiled-coil and PRY-SPRY domains. OnTRIM39 mRNA was widely expressed in various tissues. After challenge with Streptococcus agalactiae and stimulation with polyinosinic polycytidylic acid [poly (I:C)] and lipopolysaccharides (LPS), the amount of OnTRIM39 transcript was changed in various tested tissues. OnTRIM39 overexpression increased NF-κB activity. OnTRIM39 was present in the cytoplasm. Mass spectrometry of proteins pulled down with recombinant OnTRIM39 showed that 250 proteins potentially interact with OnTRIM39. The authors selected I3K4I3 from the 250 candidate proteins to verify its interaction with TRIM39. They also selected I3KL45, a member of the same 14-3-3 protein family, to verify its interaction with TRIM39. The results of pull-down assays showed that OnTRIM39 interacted with both I3K413 and I3KL45. These results contribute to further study of the innate immune mechanism of tilapia.


Assuntos
Ciclídeos , Doenças dos Peixes , Infecções Estreptocócicas , Ubiquitina-Proteína Ligases/metabolismo , Animais , Ciclídeos/metabolismo , DNA Complementar , Proteínas de Peixes/metabolismo , Regulação da Expressão Gênica , Imunidade Inata , NF-kappa B/genética , NF-kappa B/metabolismo , Poli I-C/farmacologia , Streptococcus agalactiae
5.
Dis Aquat Organ ; 149: 33-45, 2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35510819

RESUMO

The largemouth bass Micropterus salmoides is an important freshwater aquaculture fish in China. Recently, largemouth bass at a fish farm in Guangdong province experienced an outbreak of a serious ulcer disease. As part of the investigations conducted to identify the aetiology and identify potentially effective control measures, we isolated a pathogenic bacterium (NK-1 strain) from the diseased fish. It was identified as Nocardia seriolae through morphological observation, physiological and biochemical analysis, and molecular identification, and its pathogenicity was verified by experimental infection. Pathological changes in the diseased fish included granulomatous lesions in the liver and spleen, destruction of renal tubules, necrosis of intestinal epithelial cells, infiltration of inflammatory cells in the brain, vacuolation of cells, and swelling and cracking of the mitochondria and endoplasmic reticulum. Bacterial detection using qPCR showed that the spleen and intestine were the main organs targeted by N. seriolae. The mortality of largemouth bass experimentally infected with N. seriolae at 21°C was significantly lower than that in fish infected at higher temperatures between 24 and 33°C; there were no significant differences in the levels of mortality at these higher temperatures. The level of mortality of largemouth bass infected with N. seriolae was lowest at a neutral water pH of 7 but increased significantly at higher and lower pH. Of the tested Chinese herbal medicines, Chinese sumac Galla chinensis and Chinese skullcap Scutellaria baicalensis exhibited the best antibacterial effects. This study lays a foundation for the clinical diagnosis and scientific control of ulcer disease in largemouth bass.


Assuntos
Bass , Doenças dos Peixes , Nocardia , Animais , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/microbiologia , Úlcera/veterinária
6.
Electromagn Biol Med ; 41(3): 272-280, 2022 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-35438050

RESUMO

Background: In order to study the effect of bifurcation vessels parameters on the temperature field and coagulation zone of microwave ablation on lung tissue. Methods: The finite element method was used to establish the simulation model. The angle of bifurcation vessel model was 60°. The position of the antenna and the main blood vessel are parallel, and the distance between them was 5, 10 and 15 mm, respectively. Temperature field distribution was obtained at 2450 MHz, 50 W and 300 s. The blood flow velocity was set to 0.1 and 0.2 m/s. Results: The results showed when the antenna was 5 mm away from the bifurcation vessel and the velocity was 0.1 m/s, the position of x = 8.4 mm achieved the complete necrosis at 220 s, while the fraction of necrotic tissue at the symmetry point x = 1.6 mm was 0.2 at 300 s. For the distance was 10 mm and the velocity was 0.1 m/s, the fraction of necrotic tissue at x = 3 mm that near the bifurcation vessel was 0.53 and was 0.69 at the symmetry point x = 17 mm. When the antenna is 15 mm away from the vessel, the fraction of necrotic tissue of symmetrical points on both sides of the antenna obtained after ablation were the same. Conclusions: The distance between the antenna and the bifurcation vessel over 15 mm, the blood flow has no effect on the coagulation zone. Besides, the distance between bifurcation vessel and antenna possesses a greater influence on the temperature distribution and coagulation zone than the blood flow velocity.


Assuntos
Técnicas de Ablação , Ablação por Cateter , Ablação por Radiofrequência , Simulação por Computador , Fígado , Pulmão , Micro-Ondas
7.
Dev Comp Immunol ; 127: 104300, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34673140

RESUMO

Toll-like receptors (TLRs) play a critical role in the innate immune response of fish. In this study, we isolated the cDNA sequence of Nile tilapia TLR1 (OnTLR1). The deduced OnTLR1 protein contains a signal peptide, 7 leucine-rich repeats (LRRs), a C-terminal LRR (LRR-CT), a transmembrane region and a highly conserved TIR domain. In healthy Nile tilapia, the OnTLR1 transcript was broadly expressed in all examined tissues, with the highest expression levels in the spleen. After infection with Streptococcus agalactiae, the OnTLR1 transcripts were upregulated in the gill and kidney. After stimulation with polyinosinic-polycytidylic acid (poly(I:C)), the expression levels of OnTLR1 were significantly downregulated in the intestine, whereas OnTLR1 transcripts were significantly upregulated in the kidney. After challenge with lipopolysaccharide (LPS), the expression levels of OnTLR1 were significantly upregulated in the spleen and kidney. The subcellular localization showed that OnTLR1 was expressed in the cytoplasm. TLR1 significantly increased MyD88-dependent NF-κB activity. However, the results of a pull-down assay showed that OnTLR1 did not interact with MyD88 or TIRAP. Binding assays revealed the specificity of OnTLR1 for pathogen-associated molecular patterns (PAMPs) and bacteria that included S. agalactiae, Aeromonas hydrophila and poly(I:C) and LPS. Taken together, these findings suggest that OnTLR1, as a pattern recognition receptor (PRR), might play an important role in the immune response to pathogen invasion.


Assuntos
Ciclídeos , Doenças dos Peixes , Animais , Proteínas de Peixes/metabolismo , Regulação da Expressão Gênica , Imunidade Inata/genética , Fator 88 de Diferenciação Mieloide/genética , Fator 88 de Diferenciação Mieloide/metabolismo , NF-kappa B/metabolismo , Filogenia , Streptococcus agalactiae , Receptor 1 Toll-Like/genética
8.
Mol Immunol ; 132: 60-78, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33545626

RESUMO

Toll-like receptors (TLRs) play a crucial role in the innate immune system, which is the first line of defence against pathogens and pathogenic products in fish. In the present study, we cloned the full-length cDNA and genome sequences of two TLR13 s (OnTLR13a, OnTLR13b) from Nile tilapia (Oreochromis niloticus). TLR family motifs, i.e., the leucine-rich repeat (LRR) domains and Toll/interleukin (IL)-1 receptor (TIR) domains, were conserved in the putative proteins OnTLR13a and OnTLR13b, with fifteen LRR domains and one TIR domain. Four exons and three introns were identified in the OnTLR13a genome sequence, and three exons and two introns were identified in the OnTLR13b genome sequence. In healthy Nile tilapia tissues, OnTLR13a and OnTLR13b were ubiquitously expressed in all 11 tested tissues/organs. The highest expression levels were observed in the spleen (OnTLR13a) and blood (OnTLR13b), and the lowest expression levels were observed in the liver (OnTLR13a) and stomach (OnTLR13b). The expression level of OnTLR13b at 5.5 days postfertilization (dpf) was significantly higher than that at the other 8 time points (2.5, 3.5, 4.5, 5, 6, 6.5, 7.5 and 8.5 dpf). Upon stimulation with an intraperitoneal injection of 200 µL (107 CFU/mL) Streptococcus agalactiae, the expression levels of OnTLR13a and OnTLR13b were significantly upregulated in the intestine and gill. After cotransfection with MyD88, OnTLR13a significantly increased MyD88-dependent NF-κB activation in 293 T cells. However, OnTLR13b significantly impaired MyD88-dependent NF-κB activation. In addition, TLR13a slightly increased MyD88-dependent AP-1 activation, and TLR13b significantly increased MyD88-dependent AP-1 activation. TLR13a significantly increased MyD88-dependent interferon-ß (IFN-ß) activation, and TLR13b had no effect on MyD88-dependent IFN-ß activation. These findings suggest that although the deduced protein structure of OnTLR13 is evolutionarily conserved between OnTLR13 and other TLR members, its signal transduction function is markedly different. Co-immunoprecipitation (Co-IP) assays showed that both OnTLR13a and OnTLR13b could interact with OnMyD88. RNA pulldown assays showed that TLR13a and TLR13b could combine with the 23S rRNA of S. agalactiae. These results indicate that TLR13a and TLR13b play important roles in the innate immune response against bacterial infection in Nile tilapia.


Assuntos
Ciclídeos/genética , Ciclídeos/imunologia , Imunidade Inata/genética , Fator 88 de Diferenciação Mieloide/metabolismo , Streptococcus agalactiae/imunologia , Receptores Toll-Like/genética , Receptores Toll-Like/metabolismo , Sequência de Aminoácidos , Animais , Sangue/metabolismo , Ciclídeos/metabolismo , Ciclídeos/microbiologia , Éxons , Doenças dos Peixes/imunologia , Proteínas de Peixes/genética , Regulação da Expressão Gênica/imunologia , Células HEK293 , Humanos , Interferon beta/metabolismo , Íntrons , Fígado/metabolismo , NF-kappa B/metabolismo , Filogenia , Domínios Proteicos , RNA Ribossômico 23S/genética , Alinhamento de Sequência , Transdução de Sinais/imunologia , Baço/metabolismo , Fator de Transcrição AP-1/metabolismo
9.
Food Funct ; 11(3): 2639-2653, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32159193

RESUMO

Hyperoxaluria is well known to cause renal injury and end-stage kidney disease. Previous studies suggested that the renal function of rats with hyperoxaluria was improved after dietary vinegar intake. However, its underlying mechanisms remain largely unknown. The aim of the present study was to examine changes of gut microbiota and blood and urinary metabolites that associate with changes in kidney function to identify mechanisms involved with vinegar induced amelioration of hyperoxaluria-induced kidney injury. Using an ethylene glycol (EG)-induced hyperoxaluria rat model, we evaluated the effects of the vinegar on renal injury. Oral administration of vinegar (2 ml kg-1 day-1) reduced the elevated serum creatinine, BUN, and protected against hyperoxaluria-induced renal injury, renal fibrosis, and inflammation. Gut microbiota analysis of 16S rRNA gene in the hyperoxaluria-induced renal injury rats showed that vinegar treatment altered their microbial composition, especially the recovery of the levels of the Prevotella, Ruminiclostridium, Alistipes and Paenalcaligenes genus, which were significantly increased in the hyperoxaluria-induced renal injury rats. Additionally, liquid chromatography-mass spectrometry (LC-MS)-based metabolome analysis showed that total of 35 serum and 42 urine metabolites were identified to be associated with protective effects of vinegar on hyperoxaluria-induced renal injury rats. Most of these metabolites were involved in thiamine metabolism, glycerol phosphate shuttle, biotin metabolism, phosphatidylcholine biosynthesis and membrane lipid metabolism. Importantly, the effects of vinegar against renal injury were weakened after depletion of gut microbiota by antibiotic treatment. These results suggest that vinegar treatment ameliorates the hyperoxaluria-induced renal injury by improving the gut microbiota and metabolomic profiles.


Assuntos
Ácido Acético/farmacologia , Microbioma Gastrointestinal/efeitos dos fármacos , Hiperoxalúria/induzido quimicamente , Nefropatias/prevenção & controle , Administração Oral , Animais , Etilenoglicol/toxicidade , Hiperoxalúria/complicações , Nefropatias/etiologia , Masculino , Ratos , Ratos Sprague-Dawley
10.
Sci Total Environ ; 715: 136763, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32007872

RESUMO

Urban greenery is essential to the living environment of humans. Objectively assessing the rationality of the spatial distribution of green space resources will contribute to regional greening plans, thereby reducing social injustice. However, it is difficult to propose a reasonable greening policy aimed at the coordinated development of an urban agglomeration due to a lack of baseline information. This study investigated the changes in spatial fairness of the greenery surrounding residents in Guangdong-Hong Kong-Macao Greater Bay by examining time-series remote sensing images from 1997 to 2017. With the substitution of impervious, artificial surfaces for universal areas of human activities, we quantified the amount of surrounding greenery from the perspective of human activities at the pixel level by utilizing a nested buffer. The Gini coefficient was further calculated for each city to quantify the spatial fairness of the surrounding greenery to people. The results indicated that areas with less greenery surrounding them decreased during 1997 and 2017 in Guangdong-Hong Kong-Macao Greater Bay. The spatial fairness did not tend to increase with the improvements in the overall greening level. The spatial fairness of 4 cities had an increasing trend, and the Gini coefficients of 5 cities were still over 0.6 in 2017. We further proposed different greening policy suggestions for different cities based on the amount of greenery surrounding people and the trend in fairness. The results and the conclusion of this research will help to improve future regional greening policies and to reduce environmental injustice.


Assuntos
Baías , Tecnologia de Sensoriamento Remoto , Cidades , Hong Kong , Macau
11.
Fish Shellfish Immunol ; 94: 249-257, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31470139

RESUMO

In recent years, streptococcal diseases have severely threatened the development of tilapia aquaculture, but effective prevention and control methods have not yet been established. To understand the immune responses of vaccinated Nile tilapia (Oreochromis niloticus), digital gene expression (DGE) technology was applied in this study to detect the gene expression profile of the Nile tilapia (O. niloticus) liver in response to ScpB (Streptococcal C5a peptidase from group B Streptococcus, ScpB) vaccination and a Streptococcus agalactiae-challenge. The control and the ScpB-vaccinated Nile tilapia yielded a total of 25,788,734 and 27,088,598 clean reads, respectively. A total of 1234 significant differentially expressed unigenes were detected (P < 0.05), of which 236 were significantly up-regulated, and 269 were significantly down-regulated (P < 0.05, |fold|>2, FDR<0.05). Of the differentially expressed gene, the identified genes which were enriched using databases of GO and KEGG could be categorized into a total of 67 functional groups and were mapped to 153 signaling pathways including 15 immune-related pathways. The differentially expressed genes (TLR1, TLR2, TLR3, TLR5, TLR9, MyD88, C3, IL-1ß, IL-10) were detected in the expression profiles, and this was subsequently verified via quantitative real-time PCR (qPCR). The results of this study can serve as a basis for future research not only on the molecular mechanism of S. agalactiae invasion, but also on the anti-S. agalactiae mechanism in targeted tissues of Nile tilapia.


Assuntos
Ciclídeos/imunologia , Doenças dos Peixes/genética , Perfilação da Expressão Gênica/veterinária , Regulação da Expressão Gênica , Infecções Estreptocócicas/veterinária , Streptococcus agalactiae/fisiologia , Animais , Ciclídeos/genética , Regulação para Baixo , Doenças dos Peixes/imunologia , Doenças dos Peixes/microbiologia , Biblioteca Gênica , Ontologia Genética , Fígado/metabolismo , Fígado/microbiologia , Infecções Estreptocócicas/genética , Infecções Estreptocócicas/imunologia , Infecções Estreptocócicas/microbiologia , Vacinas Estreptocócicas/administração & dosagem , Regulação para Cima
12.
EBioMedicine ; 45: 231-250, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31202812

RESUMO

BACKGROUND: Epidemiological evidence of over 9000 people suggests that daily intake of vinegar whose principal bioactive component is acetic acid is associated with a reduced risk of nephrolithiasis. The underlying mechanism, however, remains largely unknown. METHODS: We examined the in vitro and in vivo anti-nephrolithiasis effects of vinegar and acetate. A randomized study was performed to confirm the effects of vinegar in humans. FINDINGS: We found individuals with daily consumption of vinegar compared to those without have a higher citrate and a lower calcium excretion in urine, two critical molecules for calcium oxalate (CaOx) kidney stone in humans. We observed that oral administration of vinegar or 5% acetate increased citrate and reduced calcium in urinary excretion, and finally suppressed renal CaOx crystal formation in a rat model. Mechanism dissection suggested that acetate enhanced acetylation of Histone H3 in renal tubular cells and promoted expression of microRNAs-130a-3p, -148b-3p and -374b-5p by increasing H3K9, H3K27 acetylation at their promoter regions. These miRNAs can suppress the expression of Nadc1 and Cldn14, thus enhancing urinary citrate excretion and reducing urinary calcium excretion. Significantly these mechanistic findings were confirmed in human kidney tissues, suggesting similar mechanistic relationships exist in humans. Results from a pilot clinical study indicated that daily intake of vinegar reduced stone recurrence, increased citrate and reduced calcium in urinary excretion in CaOx stone formers without adverse side effects. INTERPRETATION: Vinegar prevents renal CaOx crystal formation through influencing urinary citrate and calcium excretion via epigenetic regulations. Vinegar consumption is a promising strategy to prevent CaOx nephrolithiasis occurrence and recurrence. FUND: National Natural Science Foundations of China and National Natural Science Foundation of Guangdong Province.


Assuntos
Ácido Acético/administração & dosagem , Epigênese Genética/genética , Cálculos Renais/dietoterapia , Nefrolitíase/dietoterapia , Adulto , Animais , Cálcio/urina , Oxalato de Cálcio/urina , Claudinas/genética , Transportadores de Ácidos Dicarboxílicos/genética , Epigênese Genética/efeitos dos fármacos , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Histonas/genética , Humanos , Cálculos Renais/genética , Cálculos Renais/prevenção & controle , Cálculos Renais/urina , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Nefrolitíase/genética , Nefrolitíase/prevenção & controle , Nefrolitíase/urina , Transportadores de Ânions Orgânicos Dependentes de Sódio/genética , Ratos , Recidiva , Simportadores/genética
13.
Dis Aquat Organ ; 133(3): 253-261, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-31187732

RESUMO

Genetic variation in the major histocompatibility complex (MHC) Class IIB was tested in Nile tilapia Oreochromis niloticus, and the association between the MHC IIB alleles and disease resistance was also studied. F3 fry offspring (n = 1200) from 12 full-sib families were challenged with Streptococcus agalactiae, which caused significantly different mortalities in different Nile tilapia families (11.00-81.10%). Twenty fry (F1) from each of the 12 families were selected to study the polymorphisms of the MHC Class IIB gene using PCR followed by cloning and sequencing methods. The results showed that the size of the amplified fragment was 770-797 bp. Thirty-seven sequences from 240 individuals revealed 22 different alleles, which belonged to 9 major allele types. Up to 63.58% of nucleotide positions were variable, while the proportion of the amino acid variable positions was up to 68.73%. According to the survival rate of offspring (F3) from 12 full-sib families, we deduced that the alleles Orni-DAB*0107, Orni-DAB*0201 and Orni-DAB*0302 were highly associated with resistance to S. agalactiae, while the allele Orni-DAB*0701 was associated with susceptibility to S. agalactiae. In addition, our previous study found that the allele Orni-DAB*0201 was more frequently distributed in the disease-resistant groups. Therefore, the allele Orni-DAB*0201 could be used as an S. agalactiae resistance-related MHC marker in molecular marker-assisted selective breeding programs for S. agalactiae-resistant Nile tilapia.


Assuntos
Ciclídeos , Doenças dos Peixes , Infecções Estreptocócicas , Animais , Antígenos de Histocompatibilidade Classe II , Polimorfismo Genético , Streptococcus agalactiae
14.
J Fish Biol ; 93(6): 1207-1215, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30345515

RESUMO

The association between major histocompatibility complex (MHC) class IIA polymorphisms and the severity of infection by Streptococcus agalactiae was investigated using 40 susceptible and 40 resistant individuals of Nile tilapia Oreochromis niloticus. Twenty-five alleles were identified from 80 individuals, which belong to 22 major allele types. High polymorphism of mhcIIa gene and at least two loci were discovered in O. niloticus. In peptide-binding region (PBR) and non-PBR, the ratio of nonsynonymous substitution (dN) to synonymous substitution (dS) was 1.294 (>1) and 1.240 (>1), suggesting that the loci are evolving under positive balancing selection. Association analysis showed that the allele, orni-daa*0501, was significantly associated with resistance to S. agalactiae, while the alleles, orni-daa*1101, orni-daa*1301, orni-daa*1401 and orni-daa*1201, were associated with susceptibility to S. agalactiae. To confirm these correlations, another independent challenge experiment was performed in the Huizhou population of the O. niloticus. The frequency distribution showed that the orni-daa*1101 allele was significantly more frequent in the Huizhou-Susceptible group (HZ-SG) than in the Huizhou-Resistant group (HZ-RG) (P < 0.05), which was consistent with the first challenge. However, orni-daa*0501 did not present in HZ-SG and HZ-RG and the distribution frequencies of the orni-daa*1201, orni-daa*1301 and orni-daa*1401 alleles were not significantly more frequent in HZ-SG than in HZ-RG. These results indicate that the orni-daa*1101 allele confers susceptibility to S. agalactia infection. These results suggest that the diversity of exon 2 of mcaIIa alleles could be used to explore the association between disease susceptibility or resistance and the multiformity of mcaIIa and to achieve the molecular-assisted selection of O. niloticus with enhanced disease resistance.


Assuntos
Ciclídeos/genética , Resistência à Doença/genética , Doenças dos Peixes/genética , Genes MHC da Classe II/genética , Polimorfismo Genético , Infecções Estreptocócicas/veterinária , Streptococcus agalactiae , Alelos , Sequência de Aminoácidos , Animais , Ciclídeos/microbiologia , Clonagem Molecular , Antígenos de Histocompatibilidade Classe II/química , Alinhamento de Sequência , Infecções Estreptocócicas/genética
15.
Fish Shellfish Immunol ; 82: 101-114, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30099139

RESUMO

The recognition of microbial pathogens, which is mediated by pattern recognition receptors (PRRs), is critical to the initiation of innate immune responses. In the present study, we isolated the full-length cDNA and genomic DNA sequences of the MDA5, LGP2 and MAVS genes in Nile tilapia, termed OnMDA5, OnLGP2 and OnMAVS. The OnMDA5 gene encodes 974 amino acids and contains two caspase-associated recruitment domains (CARDs), a DExDc domain (DExD/H box-containing domain), a HELICc (helicase superfamily C-terminal) domain and a C-terminal regulatory domain (RD). The OnLGP2 gene encodes 679 amino acids and contains a DExDc, a HELICc and an RD. The OnMAVS gene encodes 556 amino acids and contains a CARD, a proline-rich domain, a transmembrane helix domain and a putative TRAF2-binding motif (269PVQDT273). Phylogenetic analyses showed that all three genes from Nile tilapia were clustered together with their counterparts from other teleost fishes. Real-time PCR analyses showed that all three genes were constitutively expressed in all examined tissues in Nile tilapia. OnMDA5 presented the highest expression level in the blood and the lowest expression level in the liver, while OnMAVS presented the highest expression level in the kidney. The highest expression level of OnLGP2 was detected in the liver. An examination of the expression patterns of these RIG-I-like receptors (RLRs) during embryonic development showed that the highest expression levels of OnMDA5 occurred at 2 days postfertilization (dpf), and the expression significantly decreased from 3 to 8 dpf. The expression levels of OnLGP2 significantly increased from 4 to 8 dpf. The expression levels of OnMAVS mRNA were stable from 2 to 8 dpf. Upon stimulation by intraperitoneal injection of Streptococcus agalactiae, the expression levels of OnMDA5 were first downregulated and then upregulated in the blood, gill and spleen. In the intestine and kidney, the expression of OnMDA5 was first upregulated, then downregulated, and then upregulated again. The expression of OnLGP2 was upregulated in the kidney and intestine, and the expression of OnMAVS was upregulated in the spleen. Overexpression of OnMAVS increased NF-κB activation in 293 T cells (p < 0.05), and after cotransfection with OnMDA5, the OnMAVS-dependent NF-κB activation was slightly increased (p > 0.05), after cotransfection with OnLGP2, the OnMAVS-dependent NF-κB activation was significantly decreased (p < 0.05). These findings suggest that, although the deduced protein structure of OnMDA5 is evolutionarily conserved with the structures of other RLR members, its signal transduction function is markedly different. The results also suggest that OnLGP2 has a negative regulatory effect on the OnMAVS gene. OnMDA5 and OnMAVS were uniformly distributed throughout the cytoplasm in 293 T cells, whereas OnLGP2 was distributed throughout the cytoplasm and nucleus. These results are helpful for clarifying the innate immune response against bacterial infection in Nile tilapia.


Assuntos
Ciclídeos/genética , Ciclídeos/imunologia , Proteínas de Peixes/genética , Regulação da Expressão Gênica/imunologia , Imunidade Inata/genética , Transdução de Sinais/genética , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Ciclídeos/metabolismo , Proteína DEAD-box 58/genética , RNA Helicases DEAD-box/genética , RNA Helicases DEAD-box/metabolismo , Proteínas de Peixes/metabolismo , Perfilação da Expressão Gênica , Filogenia
16.
Redox Biol ; 16: 414-425, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29653411

RESUMO

Hyperoxaluria-induced oxidative injury of renal tubular epithelial cell is a casual and essential factor in kidney calcium oxalate (CaOx) stone formation. Autophagy has been shown to be critical for the regulation of oxidative stress-induced renal tubular injury; however, little is known about its role in kidney CaOx stone formation. In the present study, we found that the autophagy antagonist chloroquine could significantly attenuate oxalate-induced autophagy activation, oxidative injury and mitochondrial damage of renal tubular cells in vitro and in vivo, as well as hyperoxaluria-induced CaOx crystals depositions in rat kidney, whereas the autophagy agonist rapamycin exerted contrasting effects. In addition, oxalate-induced p38 phosphorylation was significantly attenuated by chloroquine pretreatment but was markedly enhanced by rapamycin pretreatment, whereas the protective effect of chloroquine on rat renal tubular cell oxidative injury was partly reversed by a p38 protein kinase activator anisomycin. Furthermore, the knockdown of Beclin1 represented similar effects to chloroquine on oxalate-induced cell oxidative injury and p38 phosphorylation in vitro. Taken together, our results revealed that autophagy inhibition could attenuate oxalate-induced oxidative injury of renal tubular cell and CaOx crystal depositions in the rat kidney via, at least in part, inhibiting the activation of p38 signaling pathway, thus representing a novel role of autophagy in the regulation of oxalate-induced renal oxidative injury and CaOx crystal depositions for the first time.


Assuntos
Autofagia/genética , Oxalato de Cálcio/metabolismo , Hiperoxalúria/metabolismo , Nefrolitíase/metabolismo , Estresse Oxidativo/genética , Animais , Oxalato de Cálcio/química , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Rim/metabolismo , Rim/patologia , Túbulos Renais Distais/metabolismo , Nefrolitíase/patologia , Fosforilação , Ratos , Transdução de Sinais
17.
Fish Shellfish Immunol ; 73: 207-219, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29242132

RESUMO

The nucleotide-binding oligomerization domain proteins NOD1, NOD2 and NLRC3 are cytoplasmic pattern recognition receptors (PRRs) of the Nod-like receptor (NLR) family. In the present study, the Nile tilapia (Oreochromis niloticus) NOD1 (ntNOD1), NOD2 (ntNOD2) and NLRC3 (ntNLRC3) genes were cloned and characterized. The full-length ntNOD1, ntNOD2 and ntNLRC3 genes were 3924, 3886 and 4574 bp, encoding 941, 986 and 1130 amino acids, respectively. The three Nod-like receptors have a NACHT domain and a C-terminal leucine-rich repeat (LRR) domain. In addition, ntNOD1 and ntNOD2 have a N-terminal CARD domain (ntNOD2 has two). Phylogenetic analysis showed that the three NLRs are highly conserved. Tissue expression analysis of the three receptors revealed that the highest mRNA and protein levels of ntNOD1, ntNOD2 and ntNLRC3 were in the spleen. The expression patterns of NLRs during embryonic development showed that the expression levels of ntNOD2 and ntNLRC3 significantly increased from 2 to 8 days post-fertilization (dpf). The expression levels of ntNOD1 significantly increased from 2 to 6 dpf, decreased at 7 dpf and then increased at 8 dpf. Upon stimulation with an intraperitoneal injection of Streptococcus agalactiae, expression levels of the ntNOD1, ntNOD2 and ntNLRC3 mRNA and protein were clearly altered in the blood, spleen, kidney, intestine and gill. Furthermore, after cotransfection with an NF-κB reporter plasmid, NF-κB activation in ntNOD1-overexpressing 293T cells significantly increased compared with that in control cells, before or after i-EDPA-stimulation. By contrast, compared with control, ntNOD2 and ntNLRC3 had no effect on NF-κB activation in 293T cells, when their potential ligands were not stimulated. However, after MDP-stimulation, ntNOD2 and ntNLRC3 overexpression increased NF-κB activation in 293T cells. NOD1 and NLRC3 were uniformly distributed throughout the cytoplasm in 293T cells, whereas NOD2 was distributed throughout the cytoplasm and nucleus. Our results indicate that the three Nod-like receptors are functionally conserved and may play pivotal roles in defense against pathogens such as Streptococcus agalactiae.


Assuntos
Ciclídeos/genética , Ciclídeos/imunologia , Doenças dos Peixes/imunologia , Proteínas de Peixes/genética , Regulação da Expressão Gênica/imunologia , Imunidade Inata , Receptores de Reconhecimento de Padrão/genética , Animais , Ciclídeos/metabolismo , Proteínas de Peixes/metabolismo , Perfilação da Expressão Gênica , Peptídeos e Proteínas de Sinalização Intercelular/genética , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Proteína Adaptadora de Sinalização NOD1/genética , Proteína Adaptadora de Sinalização NOD1/metabolismo , Proteína Adaptadora de Sinalização NOD2/genética , Proteína Adaptadora de Sinalização NOD2/metabolismo , Filogenia , Receptores de Reconhecimento de Padrão/metabolismo , Infecções Estreptocócicas/imunologia , Streptococcus agalactiae/fisiologia
18.
BJU Int ; 120(1): 109-116, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28236332

RESUMO

OBJECTIVES: To investigate the prevalence and associated factors of kidney stones among adults in China. SUBJECTS AND METHODS: A nationwide cross-sectional survey was conducted among individuals aged ≥18 years across China, from May 2013 to July 2014. Participants underwent urinary tract ultrasonographic examinations, completed pre-designed and standardised questionnaires, and provided blood and urine samples for analysis. Kidney stones were defined as particles of ≥4 mm. Prevalence was defined as the proportion of participants with kidney stones and binary logistic regression was used to estimate the associated factors. RESULTS: A total of 12 570 individuals (45.2% men) with a mean (sd, range) age of 48.8 (15.3, 18-96) years were selected and invited to participate in the study. In all, 9310 (40.7% men) participants completed the investigation, with a response rate of 74.1%. The prevalence of kidney stones was 6.4% [95% confidence interval (CI) 5.9, 6.9], and the age- and sex-adjusted prevalence was 5.8% (95% CI 5.3, 6.3; 6.5% in men and 5.1% in women). Binary logistic regression analysis showed that male gender, rural residency, age, family history of urinary stones, concurrent diabetes mellitus and hyperuricaemia, increased consumption of meat, and excessive sweating were all statistically significantly associated with a greater risk of kidney stones. By contrast, consumption of more tea, legumes, and fermented vinegar was statistically significantly associated with a lesser risk of kidney stone formation. CONCLUSION: Kidney stones are common among Chinese adults, with about one in 17 adults affected currently. Some Chinese dietary habits may lower the risk of kidney stone formation.


Assuntos
Dieta/efeitos adversos , Cálculos Renais/diagnóstico por imagem , Ultrassonografia , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos Transversais , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Inquéritos Epidemiológicos , Humanos , Cálculos Renais/epidemiologia , Cálculos Renais/prevenção & controle , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Adulto Jovem
19.
Biochem Biophys Res Commun ; 479(2): 204-210, 2016 10 14.
Artigo em Inglês | MEDLINE | ID: mdl-27620488

RESUMO

Recently, ß-arrestin1 was indicated as a tumor promoter in prostate cancer, but its exact role in cancer metastasis still have not been well clarified. Here, our data revealed that ß-arrestin1 could promote the migration and invasion of prostate cancer cells via initiating epithelial-mesenchymal transition (EMT). Mechanically, ß-arrestin1 could increase the transcriptional activity and expression of ß-catenin, together with Akt activity, whereas decrease the activities of GSK-3ß and PP2A. In addition, ß-arrestin1 could function as a scaffold protein in modulating the interactions between PP2A, Akt, GSK-3ß and ß-catenin. These results reveal a novel mechanism of ß-arrestin1 in modulating EMT and GSK-3ß/ß-catenin signaling in prostate cancer, thereby suggest that assessment of ß-arrestin1 may provide a potential therapeutic target for prostate cancer.


Assuntos
Transição Epitelial-Mesenquimal , Glicogênio Sintase Quinase 3 beta/metabolismo , Transdução de Sinais , beta Catenina/metabolismo , beta-Arrestina 1/metabolismo , Western Blotting , Linhagem Celular , Linhagem Celular Tumoral , Movimento Celular , Glicogênio Sintase Quinase 3 beta/genética , Células HEK293 , Humanos , Masculino , Microscopia de Fluorescência , Neoplasias da Próstata/genética , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Proteína Fosfatase 2/metabolismo , beta Catenina/genética , beta-Arrestina 1/genética
20.
Gene ; 566(1): 23-31, 2015 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-25871516

RESUMO

Beta-defensins (ß-defensins) are small cationic amphiphilic peptides that are widely distributed in plants, insects, and vertebrates, and are important for their antimicrobial properties. In this study, the ß-defensin (Onß-defensin) gene of the Nile tilapia (Oreochromis niloticus) was cloned from spleen tissue. Onß-defensin has a genomic DNA sequence of 674 bp and produces a cDNA of 454 bp. Sequence alignments showed that Onß-defensin contains three exons and two introns. Sequence analysis of the cDNA identified an open reading frame of 201 bp, encoding 66 amino acids. Bioinformatic analysis showed that Onß-defensin encodes a cytoplasmic protein molecule containing a signal peptide. The deduced amino acid sequence of this peptide contains six conserved cysteine residues and two conserved glycine residues, and shows 81.82% and 78.33% sequence similarities with ß-defensin-1 of fugu (Takifugu rubripes) and rainbow trout (Oncorhynchus mykiss), respectively. Real-time quantitative PCR showed that the level of Onß-defensin expression was highest in the skin (307.1-fold), followed by the spleen (77.3-fold), kidney (17.8-fold), and muscle (16.5-fold) compared to controls. By contrast, low levels of expression were found in the liver, heart, intestine, stomach, and gill (<3.0-fold). Artificial infection of tilapia with Streptococcus agalactiae (group B streptococcus [GBS] strain) resulted in a significantly upregulated expression of Onß-defensin in the skin, muscle, kidney, and gill. In vitro antimicrobial experiments showed that a synthetic Onß-defensin polypeptide had a certain degree of inhibitory effect on the growth of Escherichia coli DH5α and S. agalactiae. The results indicate that Onß-defensin plays a role in immune responses that suppress or kill pathogens.


Assuntos
Ciclídeos/imunologia , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , beta-Defensinas/imunologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Ciclídeos/genética , Doenças dos Peixes/imunologia , Proteínas de Peixes/química , Modelos Moleculares , Dados de Sequência Molecular , Alinhamento de Sequência , Infecções Estreptocócicas/imunologia , Infecções Estreptocócicas/veterinária , beta-Defensinas/química , beta-Defensinas/genética
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