Acute and chronic toxicity of neonicotinoid and butenolide insecticides to the freshwater amphipod, Hyalella azteca.

Neonicotinoids are the most widely used insecticides in the world. They are preferentially toxic to insects while displaying a low toxicity toward vertebrates, and this selective toxicity has resulted in the rapid and ubiquitous use of these compounds. However, neonicotinoids have been detected in agricultural surface waters and are known to cause adverse effects in non-target aquatic organisms. A wide range of toxicity has been reported for aquatic crustaceans, but most of the studies focus on the acute effects of imidacloprid, and few data are available regarding chronic effects of other neonicotinoids or neonicotinoid replacements (e.g., butenolides). The objective of this study was to assess the acute and chronic toxicity of six neonicotinoids (imidacloprid, thiamethoxam, acetamiprid, clothianidin, thiacloprid, and dinotefuran) and one butenolide (flupyradifurone) to the freshwater amphipod Hyalella azteca. Chronic (28-d), water-only, static-renewal tests were conducted. Survival was assessed weekly, and growth was measured at the end of the exposure. Effects of neonicotinoids varied depending on the compound. Acute (7-d) LC50s were 4.0, 4.7, 60, 68, 230, and 290 µg/L for clothianidin, acetamiprid, dinotefuran, thiacloprid, imidacloprid, and thiamethoxam, respectively. Chronic (28-d) survival and growth were reduced at similar concentrations to acute (7-d) survival for thiamethoxam, acetamiprid, clothianidin, and dinotefuran. However, chronic survival and growth of amphipods exposed to imidacloprid and thiacloprid were reduced at lower concentrations than acute survival, with respective 28-d LC50s of 90 and 44 µg/L, and EC50s of 4 and 3 µg/L. Flupyradifurone was intermediate in toxicity compared to the neonicotinoids: 7-d LC50, 28-d LC50, and 28-d EC50 were 26, 20, and 16 µg/L, respectively. The concentrations of imidacloprid and clothianidin reported for North American surface waters fall within the effect ranges observed in this study, indicating the potential for these compounds to cause adverse effects to indigenous populations of H. azteca.

Lethal and sublethal toxicity of neonicotinoid and butenolide insecticides to the mayfly, Hexagenia spp.

Neonicotinoid insecticides are environmentally persistent and highly water-soluble, and thus are prone to leaching into surface waters where they may negatively affect non-target aquatic insects. Most of the research to date has focused on imidacloprid, and few data are available regarding the effects of other neonicotinoids or their proposed replacements (butenolide insecticides). The objective of this study was to assess the toxicity of six neonicotinoids (imidacloprid, thiamethoxam, acetamiprid, clothianidin, thiacloprid, and dinotefuran) and one butenolide (flupyradifurone) to Hexagenia spp. (mayfly larvae). Acute (96-h), water-only tests were conducted, and survival and behaviour (number of surviving mayflies inhabiting artificial burrows) were assessed. Acute sublethal tests were also conducted with imidacloprid, acetamiprid, and thiacloprid, and in addition to survival and behaviour, mobility (ability to burrow into sediment) and recovery (survival and growth following 21 d in clean sediment) were measured. Sublethal effects occurred at much lower concentrations than survival: 96-h LC50s ranged from 780 µg/L (acetamiprid) to >10,000 µg/L (dinotefuran), whereas 96-h EC50s ranged from 4.0 µg/L (acetamiprid) to 630 µg/L (thiamethoxam). Flupyradifurone was intermediate in toxicity, with a 96-h LC50 of 2000 µg/L and a 96-h EC50 of 81 µg/L. Behaviour and mobility were impaired significantly and to a similar degree in sublethal exposures to 10 µg/L imidacloprid, acetamiprid, and thiacloprid, and survival and growth following the recovery period were significantly lower in mayflies exposed to 10 µg/L acetamiprid and thiacloprid, respectively. A suite of effects on mayfly swimming behaviour/ability and respiration were also observed, but not quantified, following exposures to imidacloprid, acetamiprid, and thiacloprid at 1 µg/L and higher. Imidacloprid concentrations measured in North American surface waters have been found to meet or exceed those causing toxicity to Hexagenia, indicating that environmental concentrations may adversely affect Hexagenia and similarly sensitive non-target aquatic species.