Comparative assessment of aluminum and lead concentrations in serum and tissue bioptates in patients with laryngeal papilloma or cancer.

A comparative assessment of toxic element concentrations in serum and tissue bioptates in patients with laryngeal papilloma or cancer was performed. Examinations were conducted in 60 patients (40 men and 20 women) aged 20-88 years (average 59 +/- 05). Patients were divided into 3 groups; 20 patients with laryngeal papilloma were in group I, 20 with laryngeal cancer were in group II, and 20 with deviated nasal septums were included as a control group (III). Diagnosis of laryngeal papilloma (removed by direct microlaryngoscopy--Kleinsasser method) and laryngeal cancer (removed by the Rethi method) was histopatologically confirmed in patients from groups I and II, respectively. Patients in the control group received functional surgery to repair deviated nasal septums. Serum and tissue samples were obtained from all patients before surgery. Aluminum and lead concentrations were analysed by inductively coupled plasma atomic emission spectrometry (ICP-AES) using a Spectroflame M spectrometer. The considerable rise of aluminum and lead concentration in tissue bioptates and aluminum in serum in groups I and II in comparison to the control group suggests that these elements may play a significant part in the aetiology and development of precancerous lesions and laryngeal cancers. Measuring toxic chemical element concentrations in tissue bioptates can be useful in the diagnosis and estimation of development of precancerous lesions of the larynx as well as laryngeal cancer. Toxic elements concentration may play a significant role in carcinogenesis and may determine trends in cancer aetiology.

[The effect of triamcinolone, montelukast and formoterol on serum levels of il-4, IgE and clinical parameters in children with asthma]. / Wplyw acetonidu triamcinolonu, montelukastu i formoterolu na stezenia IL-4 i IgE w surowicy oraz na kliniczny przebieg astmy u dzieci.

Levels of pro-allergic cytokine IL-4 are increased in asthmatic airways, contributing to allergic inflammation. The purpose of this study was to define the effect of treatment with triamcinolon, montelukast, and formoterol on serum level of IL-4 and IgE, clinical parameters (symptom score, FEV1) and bronchial hyperreactivity (BHR) in children with moderate asthma. It was 8 week, placebo-controlled and randomized, double blind trial of 99 children with moderate atopic asthma allergic to dust mite. Patients were randomly allocated to receive 400 mg triamcinolon (n = 20), 5 or 10 mg (according to age) montelukast (n = 18), 24 mg formoterol (n = 19), or placebo (n = 42). 80 children completed the study. After treatment with triamcinolon, montelukast, and formoterol the level of IL-4 in blood serum in all study groups significantly decreased, and all clinical parameters improved; treatment with triamcinolon, formoterol, and montelukast had no effect on IgE level in serum. Mean IL-4 levels in serum before and after treatment with triamcinolon were 0.129 pg/ml with 95% Cl, 0.1-0.145 pg/ml and 0.086 pg/ml with 95% Cl, 0.023-0.109 pg/ml respectively (p = 0.02); with montelukast were 0.123 pg/ml with 95% Cl, 0.57-0.82 pg/ml and 0.102 pg/ml with 95% Cl, 0.62-0.82 pg/ml respectively (p < 0.001); with formoterol were 0.128 pg/ml with 95% Cl, 0.108-0.164 pg/ml and 0.113 pg/ml with 95% Cl, 0.096-0.146 pg/ml respectively (p = 0.002). No correlations have been found between changes in serum IL-4 and any other clinical parameters after treatment. This study demonstrates that one of the possible ways by which triamcinolon, montelukast, and formoterol contribute to inhibition of allergic inflammation is by decreasing IL-4 levels.

An orbital teratoma operated on the 2nd day after birth--a case report.

This is a case report of an intraorbital tumour found soon after delivery in otherwise normal girl. The clinical manifestation were so intensive that the operation was performed within 24 hours after birth. Histologically a benign teratoma presenting almost all possible tissues was diagnosed. In the follow up the girl's bilateral eye-function was satisfactory and she was doing well.

[Ovulation induction in sterile females with the polycystic ovary syndrome]. / Ovulationsinduktion bei sterilen Frauen mit dem Syndrom der polyzystischen Ovarien.

The outcomes of ovulation induction in 34 infertile women with polycystic gonads were presented. The ovulation was inducted by the ovarian wedge resection or conservative treatment with either clomiphen alone or in association with human chorionic gonadotropin (HCG). The procedure of choice of specific method of management was dependent on the results of laparoscopy, while an ovarian biopsy was performed and histopathological evaluation of segments. In patients with insignificantly enlarged ovarian tunica albuginea conservative treatment with hormones was carried out, in patients with either marked enlarged tunica albuginea or thecal cells in stroma ovarian wedge resection was performed. The presented method of management caused the ovulatory cycles in 85.2% of cases and pregnancies in 61.7% of cases.

Morphological changes in the brains of female patients who died in connection with pregnancy or delivery.

The authors examined 23 brains of female patients who died during pregnancy or after delivery. Four clinical groups were distinguished: 1. sepsis, 2. gestosis, 3. patients with cardiac diseases, 4. other cases of death. General autopsy on all the dead patients was performed. Macroscopically the brain revealed the signs of oedema and herniationes. Microscopic findings showed in the sepsis and gestosis groups, distinct changes in small blood vessels. Their walls were often statified and impregnated with fibrin. In the cases with the DIC syndrome microthrombosis could be seen. Only in 2 patients cerebral arterial occlusion was noted. Neurons revealed the secondary changes described as anoxic encephalopathy.

Effects of oxytocin and estradiol on volumes of the cell nuclei of the pituitary pars intermedia in male rat.

After administration of estradiol, oxytocin and both together to a male rat, investigations of the pituitary pars intermedia were performed. Diminution of the cell nuclei volumes after simultaneous administration of oxytocin and estradiol was observed. The experiment has stressed the role of both hormones in melanotropin release control.