Assuntos
Vírus da Febre Suína Africana/isolamento & purificação , Febre Suína Africana/diagnóstico , Vetores Aracnídeos/virologia , DNA Viral/química , Ornithodoros/virologia , Febre Suína Africana/epidemiologia , Febre Suína Africana/transmissão , Animais , Animais Selvagens/virologia , Sequência de Bases , Eletroforese em Gel de Campo Pulsado/veterinária , Amplificação de Genes , Dados de Sequência Molecular , Nigéria/epidemiologia , Reação em Cadeia da Polimerase/veterinária , SuínosRESUMO
A natural outbreak of Newcastle disease (ND) was reported in a flock of guinea-fowl in Nigeria, affecting 1,029 birds of which 250 (24.3%) died. Paralysis of the legs and wings, coughing, sneezing, white diarrhoea and complete cessation of egg production were observed. Serum samples collected at the onset and during the course of the disease had high ND antibody titres. ND virus was isolated from a pool of brain tissues from diseased guinea-fowl. The ND virus isolate was characterised as a velogenic strain.
Assuntos
Surtos de Doenças/veterinária , Doença de Newcastle/epidemiologia , Vírus da Doença de Newcastle/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Testes de Inibição da Hemaglutinação/veterinária , Doença de Newcastle/patologia , Vírus da Doença de Newcastle/classificação , Vírus da Doença de Newcastle/imunologia , Nigéria/epidemiologia , Aves DomésticasAssuntos
Grupos de População Animal , Animais Selvagens , Antílopes , Artiodáctilos , Búfalos , Surtos de Doenças/veterinária , Peste Bovina/epidemiologia , Suínos , Animais , Bovinos , NigériaRESUMO
Sixty-one sera from animals that had contact with Dermatophilus congolensis were examined by comparing three serological methods; counterimmunoelectrophoresis, passive haemagglutination, and agar gel diffusion, and by using four different antigenic extracts of D congolensis. The counterimmunoelectrophoresis was the most satisfactory of the methods having been found to be specific and sensitive, easy to perform and suitable for screening large numbers of samples. It was also found to have a higher antibody detection rate (82.2 per cent) than the other methods thus making it suitable for seroepidemiological surveys. It was found to be capable of detecting multiple antibodies and also revealed dissimilarities among the different antigenic extracts. The cellular antigens of D congolensis were found to detect antibody in more sera than the extracellular antigen; the cell wall extract proved to be the most satisfactory of all, detecting antibody from the largest number of sera compared to the other extracts in all the three serological tests.
Assuntos
Infecções por Actinomycetales/veterinária , Actinomycetales/imunologia , Anticorpos Antibacterianos/análise , Doenças dos Bovinos/diagnóstico , Contraimunoeletroforese , Imunoeletroforese , Infecções por Actinomycetales/diagnóstico , Animais , Bovinos , Contraimunoeletroforese/métodos , Testes de Hemaglutinação/veterinária , Imunização/veterinária , Imunodifusão/veterinária , Imunoeletroforese/métodosAssuntos
Doenças das Aves Domésticas/epidemiologia , Animais , Nível de Saúde , Nigéria , Aves DomésticasRESUMO
Differences in antibody response to three Newcastle disease virus (NDV) strains--B-1, LaSota, and Ulster--were investigated using the hemagglutination-inhibition (HI) micro-titer test in chickens hatched from ND-immune and unimmune flocks. When used singly as primary vaccines, the Ulster strain stimulated the lowest antibody response of the three in both immune and unimmune (susceptible) chickens. Subgroups of each of the primary-vaccinated groups were revaccinated with each of the three strains. Ulster-vaccinated chicks, revaccinated with Ulster, gave the poorest booster response. All other revaccination combinations gave a significant titer increase, though some were better than others. It is suggested that the Ulster strain as primary vaccine followed by booster does of B-1 or LaSota will induce a higher antibody response (i.e., immunity) in susceptible chicken populations with less risk of a post-vaccination reaction.
