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2.
Biofizika ; 50(1): 101-6, 2005.
Artigo em Russo | MEDLINE | ID: mdl-15759508

RESUMO

The influence of nanodiamonds synthesized by detonation on human red and white blood cells in vitro was studied. Nine nanodiamond samples produced by different manufacturers and two samples of modified nanodiamonds with high colloidal stability were used in the study. It was shown that nanoparticles cause the destruction of white cells and the hemolysis of erythrocytes. Possible mechanisms of the desctructive effect of detonation nanodiamonds on blood-forming elements are discussed.


Assuntos
Diamante , Eritrócitos , Hemólise , Leucócitos , Humanos , Nanotecnologia
3.
Mikrobiologiia ; 73(1): 99-104, 2004.
Artigo em Russo | MEDLINE | ID: mdl-15074048

RESUMO

The effect of mineral particulate matter on the population of bacterioplankton, its aggregation, and productive characteristics was studied in model experiments with different concentrations of particulate kaolin and the same concentration of organic substance (sodium humate). It was found that the presence of mineral particulate matter stimulated the aggregation of bacterioplankton, improved bacterial production, and extended the productive period of bacterioplankton. The integral specific production of aggregated bacterioplankton was higher than that of free-swimming bacterioplankton. The energy metabolic coefficient K2 of bacterioplankton in the presence of mineral particulate matter was higher than in its absence.


Assuntos
Bactérias/metabolismo , Plâncton/metabolismo , Bactérias/crescimento & desenvolvimento , Caulim/metabolismo , Consumo de Oxigênio , Plâncton/crescimento & desenvolvimento , Solo , Microbiologia do Solo
4.
Izv Akad Nauk Ser Biol ; (5): 534-40, 2002.
Artigo em Russo | MEDLINE | ID: mdl-12400376

RESUMO

The functional characteristics of bacterioplankton have been studied with reference to its aggregation in water bodies of different types. Several methods were used for calculation of the total numbers of bacteria and proportion of aggregated cells. Analysis of the experimental data has shown a relationship between the functional characteristics of bacterioplankton, such as bacterial production, destruction of organic matter, and energy coefficient, and the proportion of aggregated bacteria. It has been shown that as this proportion increases, the production per cell and energy coefficients also increase.


Assuntos
Bactérias , Plâncton/fisiologia , Microbiologia da Água , Animais , Fenômenos Fisiológicos Bacterianos , Ecologia , Metabolismo Energético , Água Doce , Técnicas Microbiológicas , Sibéria , Árvores
5.
Mikrobiologiia ; 70(3): 405-11, 2001.
Artigo em Russo | MEDLINE | ID: mdl-11450465

RESUMO

The dependence of the functional characteristics of bacterioplankton from the loess of Lake Khanka on the pore size of filtering materials was investigated. Soluble organic matter (SOM), bacteria, and bacterial consumers adsorbed on particles suspended in the lake water were found to filter differently depending on the pore size of the filtering material. Filters with pore size 4.5 microns (filters II) retained up to 20% of SOM and 20-30% of bacterial cells. Filters III with pore size 2.87 microns retained almost 50% SOM and about 40% of bacteria. The double layer of gauze no. 72 (referred to as filter I) with pores size 40 microns was unable to completely retain bacterial consumers. In the case of filtrates I and II, the generation time of bacterioplankton decreased with its increasing average daily concentration. In the case of filtrate III, the generation time of bacterioplankton was minimum and did not depend on its concentration. Oxygen consumption rates per one bacterial cell and per unit biomass in filtrates increased with decreasing pore size of the filters through which they had passed. The bacterial biomass and oxygen consumption rate increased exponentially in filtrates III and logarithmically in filtrates I.


Assuntos
Bactérias/isolamento & purificação , Plâncton/isolamento & purificação , Microbiologia da Água , Animais , Bactérias/metabolismo , Contagem de Colônia Microbiana , Filtração , Água Doce , Oxigênio/metabolismo , Plâncton/metabolismo , Plâncton/fisiologia , Federação Russa
6.
Mikrobiologiia ; 69(4): 559-64, 2000.
Artigo em Russo | MEDLINE | ID: mdl-11008693

RESUMO

Some characteristics of bacterioplankton--generation time, daily (P) and specific (P/B) bacterioplankton production, and bacterial metabolic coefficient K2--in the loess-containing Lake Khanka were determined using five modifications of the bacterial-count procedure with the fluorescent dyes fluorescamin and erythrosin. Experiments showed that the organomineral complex (OMC) in this lake is broken down by chemoorganoheterotrophic bacteria. The increase in the loess content of the lake water intensified bacterial growth and the cycles of potassium, silicon, and other biogenic elements. The addition of starch to a loess suspension activated the breakdown of OMC due to the adsorption of starch on the OMC/water interface and stimulation of the metabolism of attached bacteria.


Assuntos
Fenômenos Fisiológicos Bacterianos , Plâncton/fisiologia , Animais , Bactérias/isolamento & purificação , Contagem de Colônia Microbiana , Corantes Fluorescentes , Água Doce , Plâncton/isolamento & purificação
7.
Gematol Transfuziol ; 36(9): 19-22, 1991 Sep.
Artigo em Russo | MEDLINE | ID: mdl-1769504

RESUMO

Repeated plasmapheresis sessions attended by chemiluminescent evaluation of cellular immunity parameters lead to the recovery of the whole blood leucocyte activity in patients with unhealing gastroduodenal ulcers. The kinetic analysis of the cellular chemiluminescent response has shown that, among all the components of the plasmapheresis procedure, hemorrhage is the most important factor increasing the functional activity of phagocytes.


Assuntos
Leucócitos/imunologia , Medições Luminescentes , Plasmaferese , Humanos
9.
Biull Eksp Biol Med ; 107(1): 62-4, 1989 Jan.
Artigo em Russo | MEDLINE | ID: mdl-2783654

RESUMO

The presence of antigenic markers of T-lymphocytes (Thy-1, HKO-10, HKO-11 antigens) in phenotype of normal killers of autologous erythrocytes (NKAE) has been shown. Heterogeneity of NKAE in respect to sensitivity to PHA activating the cytotoxic activity of NKAE of mouse thymus and NKAE of people peripheral blood, but not NKAE of mouse bone marrow has been registered. A part of lymphocytes of T-row is supposed to possess the NKAE-function and to be responsible for homeostasis of red sprout of normal hemopoiesis.


Assuntos
Eritrócitos , Células Matadoras Naturais/imunologia , Animais , Anticorpos Monoclonais/imunologia , Medula Óssea/imunologia , Células da Medula Óssea , Testes Imunológicos de Citotoxicidade , Eritropoese , Feminino , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Fenótipo , Coelhos , Linfócitos T/imunologia , Timo/citologia , Timo/imunologia
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