[Immunoassay and discriminant analysis to improve the diagnosis of ovarian cancer in the elderly.]

Ovarian cancer is a common pathology in the structure of tumors among elderly women. The aim of the work is to identify the leading indicators of interleukin profile to improve the diagnosis of ovarian cancer in the elderly. Enzyme immunoassay studied serum levels of interleukins in 78 patients with ovarian cancer stage IIB-IIIB according to the classification of FIGO at the age of 65-70 years and 42 women of similar age without ovarian cancer at the time of the study and in history. The differentiation of the compared groups is carried out by means of discriminant method. The leading diagnostic interleukins for ovarian cancer in the elderly are IL-1α, IL-1ß, IL-8 and TNF-α. The constructed discriminant models allow to differentiate patients with ovarian cancer and control women with an accuracy of 93.2-93.8%. The use of enzyme immunoassay and discriminant analysis improve the diagnosis of ovarian cancer in the elderly.

[The application of flow cytometry technique and diagnostically significant indices of systemic cellular immunity for diagnostic of acute endometritis.]

The acute endometritis is a the most widespread pathology among women of reproductive age.The immune system plays a leading role in its development. However, there is no data concerning the most informative parameters of cell immunity. The purpose of study is to enhance diagnostic of acute endometritis using immunologic technique with objective evaluation of diagnostic value of indices of systemic cell immunity. The indices of cell immunity are analyzed on sampling of 78 patients with postnatal acute endometritis and 54 healthy women. The technique of flow cytometry was applied with subsequent calculation of index of informativeness and disintegration objectifying selection of diagnostically significant parameters. It is demonstrated that full-fledged diagnostic of acute endometritis requires detection of absolute and relative content of T-helpers and natural killers having high value of informativeness and disintegration. Hence an opportunity to reduce number of analyzed parameters of systemic cell immunity and financial costs of diagnostic of acute endometritis.

[The differentiated diagnostic of chronic salpingoophoritis and ovary cancer on the basis of immunologic and discriminant techniquea.]

The ovary cancer and chronic salpingoophoritis are among leading pathologies of female's sexual sphere and a main cause of morbidity and mortality of female population. The purpose of study was to select leading differential diagnostic indices of systemic cellular immunity and to carry out a differentiation of patients with ovary cancer and chronic salpingoophoritis using discriminant technique. The differentiated diagnostic of ovary cancer and chronic salpingoophoritis was applied to 92 and 87 patients correspondingly to indices of systemic cellular immunity and analyzed by flow cytometry technique. It is demonstrated that the main differential diagnostic indices of systemic cellular immunity in case of ovary cancer and chronic salpingoophoritis are decreasing of relative content of natural killers, absolute number of B-lymphocytes and T-helpers. The developed discriminant models permit to carry out in 91.8%-92.1% cases a faultless differentiated diagnostic of ovary cancer and chronic salpingoophoritis. The approach like that increases quality of differentiated diagnostic of ovary cancer and chronic salpingoophoritis.