Intraspecific variation of morphological traits backed up with molecular evidence votes for re-appraisal of hitherto distinguished Balaustium species-a case study of Balaustium murorum (Acariformes: Parasitengona, Erythraeidae).

Molecular examination of representatives of Balaustium from several populations in SW Poland, performed using the sequence data from the mitochondrial cytochrome c oxidase subunit I, confirmed their common specific affiliation and identity with Balaustium murorum. The potential presence of distinct species in the studied material, preliminarily inferred from the discovery of clusters as a result of Principal Component Analysis exploring the metric data sets, was rejected due to the finding of only one haplotype, at intra- and inter-population sampling. An insight into meristic traits in larvae, focused on chaetotaxy of legs, revealed wider variation than hitherto recognized for the species. The variation was higher in laboratory-reared larvae compared to field-collected ones. The overall deviations from the mean character values at intra- and interpopulation levels, higher than hitherto observed for the species, vote for the reappraisal of the criteria adopted for discrimination of members of Balaustium with the application of an integrative approach.

Let me know your name: a study of chigger mites (Acariformes: Trombiculidae) associated with the edible dormouse (Glis glis) in the Carpathian-Balkan distribution gradient.

Trombiculid mites were collected from the edible dormouse (Glis glis) within the Carpathian-Balkan distribution gradient of host species. Representatives of five genera (Leptotrombidium, Neotrombicula, Brunehaldia, Hirsutiella, Schoutedenichia) and 10 species of chiggers were discovered in the material, based on morphological and/or molecular data. Brunehaldia, new to the fauna of Greece, was recorded for the first time from the edible dormouse. Neotrombicula talmiensis was new to the fauna of Greece and Neotrombicula vulgaris was new to the fauna of North Macedonia. Successful amplification and sequencing of COI was carried out in relation to three genera and six species. The intraspecific variation of taxa hitherto distinguished based on morphological criteria was juxtaposed with molecular data, using the distance method and the phylogenetic approach. The molecular methods indicated wider than hitherto recognized, intraspecific morphological variation for Leptotrombidium europaeum and N. talmiensis. On the other hand, an inference limited to morphology proved to be insufficient for species delineation, which was confirmed by the relatively low identity (%) of examined COI sequences as well as the size of inter-/intraspecific K2P distance threshold. Our study provides support for integrative taxonomy that combines different sources of evidence and contributes to recognition of the scope of intraspecific variation. The high degree of hidden diversity revealed with the application of molecular tools, votes for a careful approach to the identification of chiggers. The confirmed cases of co-invasion, including the representatives of various genera (Leptotrombidium and Neotrombicula, Brunehaldia and Neotrombicula, Neotrombicula and Schoutedenichia, Hirsutiella and Schoutedenichia) additionally support the need to include all larvae found on a given host specimen in the identification process.

An overview of chelicerate ovaries, with special reference to mites - myths and facts.

In arthropods of the subphylum Chelicerata a panoistic ovary, in which all germline cells differentiate into oocytes, prevails. Among the chelicerates, mites are believed to show a great variety of the structure of the female gonads. In general, the knowledge of the ovarian structure in mites is fragmentary and patchy. In both evolutionary lines, Acariformes and Parasitiformes, apart from the panoistic ovary, the meroistic ovary, in which the oocytes grow supported by their sibling cells, the nurse cells, occurs. The presence of the meroistic ovary is considered an apomorphic state. Previous studies revealed a various structure of the meroistic ovary in different mite taxa, and the differences came down, inter alia, to a different number and location of the nurse cells in relation to the oocytes. Here we provide a comprehensive review of the structure of the Chelicerata ovary, with special reference to the mite ovary. We also provide our preliminary results of the analysis of ovarian structure in two representatives of terrestrial Parasitengona (Acariformes), Allothrombium fuliginosum (Trombidiidae) and Erythraeus cinereus (Erythraeidae), performed using light, confocal and electron transmission microscopy. The analyses allowed for verification of data published before. In A. fuliginosum we showed the presence of the nurse cells in the ovarian wall, so the ovary should be classified as meroistic. In meroistic ovary of E. cinereus we found that each oocyte is connected to several mononucleated nurse cells. The verification of literature data and broadening the knowledge of the structure of the female gonad in mites, will result in estimating the usefulness of the ovary traits in phylogenetic analyses and will provide the basis for inference about the directions of evolutionary changes of female gonad at lower systematic levels.

Chigger mite (Acariformes: Trombiculidae) infestation in reed passerine birds in Central Europe: a case of the bearded tit Panurus biarmicus.

Larval trombiculid (chigger) mites are common ectoparasites of terrestrial vertebrates including humans, causing itching and skin inflammation known as trombiculiasis. Investigation of their diversity, distribution and seasonal abundance is therefore important from a veterinary and public health point of view. Although researchers have paid increased attention to these parasites in recent years, there is still little ecological data available on chiggers associated with birds inhabiting different types of habitats such as wetlands, for example. In 2021, we investigated the mite fauna in a specialist reedbed passerine, the bearded tit (Panurus biarmicus), and their effects on this host in the south-west Slovakia, Central Europe. A total of 1134 larvae of 1 mite species Blankaartia acuscutellaris were found in 99 out of 267 examined bearded tits. Juveniles were more infested than adult birds, but no differences were found between sexes. The larvae of mites first appeared on the host during the second half of June and peaked in the second half of July. After that, their numbers decreased gradually until October. Despite the relatively high prevalence and intensity of mite infestation in the bearded tit, no differences in body condition between infested and uninfested birds suggest that infestation by B. acuscutellaris may not have serious negative effects on the host health. Bearded tits can therefore be a reliable indicator of the presence of the chigger mites in wetland habitats.

Inclusion of juvenile stages improves diversity assessment and adds to our understanding of mite ecology - A case study from mires in Norway.

Arachnid orders, Mesostigmata, Trombidiformes, and Sarcoptiformes, commonly known as 'mites', are abundant in mires, both as adults and as juveniles. However, due to the challenges of identification, the juvenile forms are often excluded from analyses. This is the first study in mires that included all three mite orders identified to the species level, including juvenile instars. We aimed to compare how diversity and the response to ecological variables differed if only the adults (ad) vs. the total number of specimens (ad+juv) are considered. Samples of 20 Sphagnum species (five subgenera) were collected and mites were extracted using Berlese funnels. Overall, nearly 60,000 mites were analyzed; of these Mesostigmata made up 1.87% of the total, Trombidiformes -0.27%, and Sarcoptiformes -97.86%. The study revealed 154 species (33 Mesostigmata, 24 Trombidiformes, and 97 Sarcoptiformes), the highest diversity of mites ever reported from mires. The inclusion of juveniles increased observed species richness by 6%, with 10 species (one Mesostigmata, six Trombidiformes, and three Sarcoptiformes) represented only by juvenile forms. Seventeen species are new to Norway (four Mesostigmata, one Sarcoptiformes, and 12 Trombidiformes, including five undescribed species of Stigmaeidae and Cunaxidae). Four of these were represented in the samples only by juveniles. Including the juveniles explained a greater amount of the variability of Trombidiformes (explanatory variables account for 23.60% for ad, and 73.74% for ad+juv) and Mesostigmata (29.23% - ad, 52.91% - ad+juv), but had less of an impact for Sarcoptiformes (38.48% - ad, 39.26% - ad+juv). Locality, Sphagnum subgenus and species, wetness, and trophic state significantly affected the mite communities and should be taken into consideration when studying mires. Since juvenile stages contribute significantly to mite diversity in mires, they should also be included in mite studies in other habitats.

Parasitism, seasonality, and diversity of trombiculid mites (Trombidiformes: Parasitengona, Trombiculidae) infesting bats (Chiroptera) in Poland.

The study aims to ascertain the diversity of trombiculid species associated with Chiroptera in Poland, and for the first time in the case of research on Central European Trombiculidae, we use both DNA and morphology in an integrative taxonomic approach to determine species identities of trombiculids. The research was carried out from 2015 to 2019. In total, 2725 larvae were collected from 300 specimens of bats belonging to 11 species. Deutonymphs were obtained through laboratory rearing of larvae; few larvae and deutonymphs were collected also from bats' daily roosts. The presence of trombiculid larvae on hosts was observed between July and April of the following year, with the highest numbers recorded in autumn, during bat swarming. Male bats were infested more often than females (16.4 vs. 6.6%). The highest infestation rate was recorded for Barbastella barbastellus, Myotis nattereri and Plecotus auritus, and the highest prevalence of chiggers (> 30%) for Myotis bechsteinii and P. auritus. The larvae found on bats occupied the areas with free access to the host's skin: auricles, tragus, and snout. Morphological identification of specimens to the species level was hindered by the mosaic distribution of diagnostic traits. Morphological analyses indicated the presence of Leptotrombidium russicum and Leptotrombidium spp. in the examined material, whereas molecular analyses additionally suggested three other potential species assigned to the same genus based on the assessed scope of intrageneric variation (ASAP method). We argue that the identification of the parasitic larvae (chiggers) using morphological characters does not address the question of actual species boundaries, which, in turn, affects the inferences about host specificity and host range.

Tyrophagus putrescentiae (Sarcoptiformes: Acaridae) in the in vitro cultures of slime molds (Mycetozoa): accident, contamination, or interaction?

Tyrophagus putrescentiae (Schrank), commonly known as the cereal mite, cheese mite, or ham mite, is a cosmopolitan species reported from various environments in the wild, including soil, plant material and vertebrate nests. It has also been recognized as a common pest of food storages, mycological collections as well as plant and invertebrate laboratory cultures. Laboratory observations indicate that T. putrescentiae feeds on a large range of dermatophytes, yeasts and molds. We have observed the interspecific relation between this mite and several species of true slime molds (Mycetozoa) under laboratory conditions, which confirms the very broad spectrum of feeding habits of T. putrescentiae. Mycetozoans were grown in semi-sterile in vitro cultures and fed with oat flour or oat flakes. Tyrophagus putrescentiae displayed affinity to all macroscopically identifiable stages of the life cycle of Fuligo septica (L.) F.H. Wigg, Physarum polycephalum Schwein and the Didymium sp. complex [Didymium iridis (Ditmar) Fr., Didymium nigripes (Link) Fr. and Didymium bahiense Gottsb.]: live, decaying or dead plasmodia, sporangia, aethalia, spores and sclerotia. The relation carrying symptoms of various types of interspecific interaction, is hypothesized to form an evolutionarily young phenomenon, which not only identifies a new aspect of mycetozoal biology, but also presents the cereal mite as a species of high adaptive potential.

Stylostome formation by parasitic larvae of Allothrombium fuliginosum (Trombidiformes: Trombidiidae): morphology of feeding tubes and factors affecting their size.

The morphology and formation of stylostomes (feeding tubes) in hosts' body during the parasitic phase of Allothrombium fuliginosum (Hermann) larvae were studied for the first time with light microscopy (LM) and transmission electron microscopy (TEM). The stylostomes were observed in three aphids species-Acyrthosiphon pisum (Harris), Elatobium abietinum (Walker), and Macrosiphum rosae (L.)-parasitized by mites under laboratory conditions. They consisted of 2-6 main branches, preliminarily unbranched, then producing secondary and sometimes also tertiary branches as finally formed structures. Their walls were uniformly electron-dense, without any longitudinal and transverse stratifications and showed rather irregular outlines. Distally, the stylostome branches revealed transparent pores and cavities in their walls, connecting the stylostome canal with surrounding haemocoelic space. The total length of stylostomes at the end of the parasitic phase was on average 16× greater than that recorded in the youngest stylostomes. No differences in the overall shape of feeding tubes between host species were stated. The stylostomes formed in different host species did not differ significantly, except their total length, which attained the highest value in tissues of Ac. pisum.

Active postlarval forms of plume-footed Eatoniana (Trombidiformes: Parasitengona, Erythraeidae) in the Eocene Baltic amber.

The discovery of active postlarval forms (deutonymph and adult) of erythraeid Eatoniana in Eocene Baltic amber is associated with the first description of named fossil species assigned to that genus. Species of Eatoniana, having the plume-like setae on terminal segments of leg IV, reveal the high consistency of characters, confirmed both for extinct and extant members of the genus. The main difference between Eatoniana crinita sp. nov. and other "plume-footed" congeners is the shape of modified setae arising at genu and tibia IV.

Inter- and intraspecific variability of morphological and molecular characters in Allothrombium species, with special reference to Allothrombium fuliginosum.

Morphology-based identification of Allothrombium spp., in view of the limited knowledge of intraspecific variation, hinders the recognition of species borders and affects the views on the actual distribution of species. Therefore, identification will benefit from reference to molecular methods. The separate species identity of specimens putatively representing Allothrombium fuliginosum and A. pulvinum, both reported as widely distributed in the Palaearctic region and considered as potential biological control agents, was checked using morphological and molecular analyses. The representatives of various Allothrombium spp. collected in the Palaearctic were included in the analysis in order to ascertain the distance between species. The results of the morphological examination, supported by statistical inference, along with the comparison of COI and/or ITS2 sequences, weaken the hypothesis of synoccurrence of both species in the Palaearctic region. Hence, we hypothesize that A. fuliginosum is widely distributed in the Palaearctic, whereas A. pulvinum should be regarded a Nearctic species.

Checklist of terrestrial Parasitengona mites in Fennoscandia with new species- and distribution records (Acariformes: Prostigmata).

The knowledge of terrestrial Parasitengona in Fennoscandia lies far behind that of their aquatic counterparts, the water mites (Hydrachnidia). Based on new inventories, we provide primary data and an annotated checklist of terrestrial Parasitengona in Fennoscandia including 107 species. Out of these, nineteen species are new findings for the region and five are species potentially new for science. Twenty-three species are new for Norway, fourteen for Finland and eleven for Sweden. The known recorded fauna today of terrestrial Parasitengona is 80 species for Norway, 54 for Sweden and 48 for Finland. Primary data include georeferenced locality data as well as collecting techniques and microhabitat to increase the knowledge on species' habitat requirements.

Evidence for co-invasion events: different chigger species (Actinotrichida, Trombidioidea: trombiculidae) share a host.

Cases of co-invasion of various chigger species parasitizing murids and cricetids in various habitats were analysed using morphological and molecular approaches. Here we provide evidence for 25 new cases of co-parasitism of chigger mites on rodent hosts (Myodes glareolus, Apodemus flavicollis, Apodemus agrarius) accounting for 8.6% of all host-parasite associations observed in this study. The results confirm higher incidence of co-parasitism in vertebrate-associated Parasitengona mites compared to arthropod-associated ones. Among factors influencing the occurrence of co-parasitism in Trombiculidae the body constitution and year-round availability of hosts associated with lower host specificity of larvae should be considered.

Host-parasite association in trombiculid mites (Actinotrichida: Trombiculidae) of temperate zone - the case of Hirsutiella zachvatkini (Schluger, 1948); are we dealing with prolonged contact with the host?

BACKGROUND: The time-extended contact of trombiculid larvae with hosts poses a question of its ecological determinants. The phenomenon, which may facilitate the overwintering of larvae in the temperate zone, was previously observed in few parasitengone taxa, but not confirmed for mammal-associated trombiculids. The study aims at tracing the phenology of larvae of Hirsutiella zachvatkini and at verifying the hypothesis of contact with the host, extending beyond the parasitic phase. METHODS: Apodemus agrarius, Apodemus flavicollis and Myodes glareolus, trapped during 2-year studies, were checked for the presence of trombiculid larvae. Larvae of H. zachvatkini served for the studies. The degree of mites' engorgement was checked over time in order to estimate the duration of feeding phase and to measure the maximum size increase. The experimental rearing aimed at ascertaining the relations between the level of engorgement and successful transformation of larva into subsequent instar. RESULTS: The mass appearance of larvae on hosts fell on autumn and winter, with a decrease observed in spring, leading to an almost total absence in early and mid summer. The highest intensity, attained in late autumn or in winter, was not followed by further increase in the number of host-associated larvae. The percentage of unengorged larvae on hosts was disproportionately small, irrespective of the season. The size increase of larva was 12.6-fold at maximum. Engorged or partly engorged larvae, observed from the beginning of mass appearance over the entire period of host-parasite association in the field, transformed into subsequent instar when removed from host. CONCLUSIONS: An increase in intensity observed from the onset of appearance of larvae on hosts, through autumn and winter months, at rarity of observations of unengorged larvae and absence of engorged larvae off-host, indicates a prolonged contact with hosts, aimed at synchronisation of life cycle, conditioned by food resources available for active postlarval forms and constitutes a strategy enabling larvae to survive the unfavourable winter conditions. The proportion of engorged and partly engorged vs. unfed larvae, observed over the survey, along with their ability to transform into subsequent instars, indicates a relatively short feeding phase. The lack of continuous increase in abundance and intensity towards spring and summer suggests a gradual detachment of partly and fully engorged larvae which attained the readiness to subsequent development. The size increase of larvae during their parasitic phase does not corroborate the neosomy in H. zachvatkini. Host-associated differences in topic preferences of the chiggers become less obvious at maximum infection rates. Quantitative descriptors of parasite population place M. glareolus among the most infected hosts of H. zachvatkini in contrast to Apodemus mice collected in the same habitat.

The first description of a microtrombidiid mite (Actinotrichida: Prostigmata, Microtrombidiidae) from Baltic amber, with notes on related extant genera and species.

Discovery of parasitengone mites (Acari) in the Gulf of Gdansk deposits of Baltic amber ("Blue Earth" sediment) resulted in the first description of a fossil representative of Microtrombidiidae. The new species, based on larvae, displays affinity to recent members of Montenegtrombium Saboori and Pesic, 2006, Persianthrombium Sedghi, Saboori and Hakimitabar (in Sedghi et al. 2010) and Porttrombidium Haitlinger, 2000, known from the southwestern Palaearctic. A comparison with related genera and species places the newly described taxon in Porttrombidium (as Porttrombidium gedanense sp. nov.). Montenegtrombium is regarded as a junior synonym of Porttrombidium.

Morphological and molecular characteristics of Milandanielia intermedia (Feider, 1950) (Trombidiformes: Microtrombidiidae) with data on its biology and ecology.

Milandanielia intermedia (Feider, 1950) is re-described. A female from which larvae were obtained by experimental rearing is designated as neotype. Verified diagnoses of active life instars are supplemented with data on habitat preferences, phenology and life cycle. Molecular identification of the species based on its COI sequence is provided.

Host-associated differences in morphometric traits of parasitic larvae Hirsutiella zachvatkini (Actinotrichida: Trombiculidae).

Examination of host-associated variation in the chigger mite Hirsutiella zachvatkini (Schluger) revealed morphological differences among larvae infesting sympatric hosts: Apodemus agrarius, Apodemus flavicollis and Myodes glareolus. The analysis included 61 variables of larvae obtained from their gnathosoma, idiosoma and legs (measurements and counts). Statistically significant differences were observed for metric characters of the legs as opposed to the scutum. In view of the conspecificity of the mites, supported by comparison of COI gene products obtained from larvae and laboratory-reared deutonymphs, the observed variation is attributed to phenotypic plasticity. The knowledge of larval morphology, including intraspecific variation of metric characters, supported by molecular and host range data, places H. zachvatkini among the most comprehensively defined members of Trombiculidae.

Parasitism of Trombidium brevimanum larvae on agrobiont linyphiid spiders from Germany.

An experiment on three differently-managed agricultural fields in Ahlum, Germany, which aimed at establishing the impact of different management systems on the biodiversity of predators and decomposers, yielded a significant number of spiders parasitized by larvae of Trombidium brevimanum (Actinotrichida, Parasitengona, Trombidiidae). Spider data from the whole sampling period (September 2010-July 2012), indicated that ectoparasitic larvae were recorded only on spiders in pitfall traps in the period of June-July 2011. In this period, only eight species of Linyphiidae--out of 42 species assigned to nine spider families recorded from the study area--were parasitized by mites; considerable levels of parasitism were recorded on Erigone atra, E. dentipalpis, and Oedothorax apicatus. The highest prevalence of parasitism was recorded on the organic field for E. atra (29%), while on the integrated and conventional fields significantly fewer parasitized spiders were observed. The preferred attachment sites on the spider host were regions with softer cuticle, especially regions on the carapace and on the abdomen, adjacent to the pedicel.

Abalakeus Southcott, 1994 is a junior synonym of "plume-footed" Eatoniana Cambridge, 1898 (Trombidiformes, Erythraeidae)--evidence from experimental rearing.

Eatoniana Cambridge, 1898 was previously known exclusively from active postlarval forms. In the course of experimental rearing, larvae showing a strong affinity to members of Abalakeus jahromiensis Sedghi, Saboori et Hakimitabar, 2010, were obtained from a field-collected female of Eatoniana plumipes (L. Koch, 1856). Studies of all members of Abalakeus compared with newly obtained larvae of Eatoniana, resulted in synonymisation of Abalakeus Southcott, 1994 with Eatoniana and of A. jahromiensis with E. plumipes. Complementary data to the previous diagnosis of Eatoniana and information on the biology of E. plumipes are provided. A female of E. plumipes was selected as the neotype. A key to Eatoniana spp. known from larvae is provided.

Planthopper (Hemiptera: Flatidae) parasitized by larval erythraeid mite (Trombidiformes: Erythraeidae)-a description of two new species from western Madagascar.

Descriptions of Dambullaeus adonis Makol et Moniuszko SP NOV: (Trombidiformes: Erythraeidae, Callidosomatinae) and Latois nigrolineata Swierczewski et Stroinski SP NOV: (Hemiptera: Fulgoromorpha, Flatidae) from Madagascar are provided. The first host record for ectoparasitic larvae of Dambullaeus Haitlinger, 2001 and the first evidence on host-parasite association between flatid adult and erythraeid larvae are given. Genus Dambullaeus, known exclusively from larvae and now comprising two species of Gondwanan distribution, is critically reappraised.

Chigger mites (Actinotrichida: Parasitengona, Trombiculidae) of Poland. An updated distribution and hosts.

The existing body of knowledge regarding the Trombiculidae of Poland is summarized and supplemented with the results of our recent studies. Although around 3000 nominal species are known worldwide, only 18 have been recorded in Poland. Due to the medical and veterinary importance of parasitic larvae, and the complex life cycle which presents difficulties in finding habitats occupied by postlarval forms, most species have been described exclusively from their larvae. This review provides updated information on the host spectrum and distribution of all the trombiculid species hitherto recorded in Poland, supplemented with data on their general distribution and biology.