[Using marker of the mitochondrial pore opening in diagnostics of patients with myocardial ischemic lesions].

In the article a possibility of definition of level of the mitochondrial factor (MF) for early diagnostics of the myocardial ischemic damages at patients during heart surgery was investigated. The reperfusion damages of heart were manifested by MPTP activation and MF release. The rising of MF level during a cardioplegia is comparable to level, which has been noted at patients with angina before operation of aorto-coronary shunting. The beginning of reperfusion, practically did not change MF level, but 5 minutes after restoration of a blood flow and warm reductions it increased to the maximum values. Thus, it closely correlated with a level of a myocardial contractility depression and data of biochemical researches, which are traditionally used in clinic for diagnostics of damages of a heart. It allows to confirm, what exactly reperfusion is a major factor damaging of a myocardium. Its important that, change of MF level we registered at first minutes of heart reperfusion, those are much earlier, than by means of other markers. It seems to us, that investigation of a MF is very perspective for early finding of an ischemic damages of different tissues and organs.

[Early marker of myocardial injury of the ischemia-reperfused heart in dogs and during operations with artificial circulation in humans].

The model of ischemia/reperfusion was reproduced on 9 unconscious dogs. Simultaneously with registration of indexes heart work, hemodynamic and mitochondrial factor (MF) in venous blood from right atrium was defined. Measures were done by spectrophotometria. We have also performed spectrophotometric determination of MF in 11 patients in course of operation with blood cardioplegia. Samples of mixed venous blood from the right atrium were taken on different stages of artificial blood circulation: ischemia and reperfusion. Besides that, patients' level of enzymes was defined: creatine kinase (CK), mv-creatine kinase (mv-CK), lactatedehydrogenase (LDG), aspartataminotransferase (AST), within first day of postoperative period, and also ECG-recording within pre- and after operative period were done. Maximal MF level correlates with frequency and severity of cardiac rhythm disturbance, severity of myocardial hypoxia (r = 0.81). Maximum MF level also demonstrated correlation with KK (r = 0.97), mvKK (r = 0.92), LDG(r = 0.81), AST (r = 0.85). Thus, in experimental and clinic conditions myocardial ischemia was accompanied by mPTP activation, which led to reperfusion myocardial injuries and to release of MF. Method of MF determination gives opportunity to propose its usage as early marker of ischemic injuries and also as marker of mPTP opening in vivo.

[The heart pumping function and the systemic regulation of blood circulation in groups of heart surgery patients]. / Nasosna funktsiia sertsia ta systemna rehuliatsiia krovoobihu v hrupakh kardiokhirurhichnykh khvorykh.

General properties of cardiovascular system functioning in the groups of postoperative patients in the cardiac surgery clinic were studied using the information technology (IT), combined the methods of cluster and nonlinear regression analysis and computer simulation. It was shown, that in all five separated groups of observations (clusters) the direct dependency of cardiac (CI) and stroke (SI) indexes on central venous pressure (CVP) was determined by the variations of total peripheral resistance and reactions of venous tone (u), contractility (E) and heart rate (HR), oriented on the stabilisation of mean aortic pressure (AP) on approximately the same level. Significant differences between the characteristics CI(CVP), SI(CVP) in the groups were determined by the level of E (especially of the right ventricle) and diastolic tone of the heart. Correlation of the cardiac (E,HR) and vascular (u) components of Ap regulation changed correspondingly the heart functional state. Proposed approach and IT may be used in such clinical and experimental research where considerable volume of observations is combined with the great variation of data and, correspondingly, a poor definition level of system behavior.