Biomechanical Sensing Using Gas Bubbles Oscillations in Liquids and Adjacent Technologies: Theory and Practical Applications.

Gas bubbles present in liquids underpin many natural phenomena and human-developed technologies that improve the quality of life. Since all living organisms are predominantly made of water, they may also contain bubbles-introduced both naturally and artificially-that can serve as biomechanical sensors operating in hard-to-reach places inside a living body and emitting signals that can be detected by common equipment used in ultrasound and photoacoustic imaging procedures. This kind of biosensor is the focus of the present article, where we critically review the emergent sensing technologies based on acoustically driven oscillations of bubbles in liquids and bodily fluids. This review is intended for a broad biosensing community and transdisciplinary researchers translating novel ideas from theory to experiment and then to practice. To this end, all discussions in this review are written in a language that is accessible to non-experts in specific fields of acoustics, fluid dynamics and acousto-optics.

Neural echo state network using oscillations of gas bubbles in water.

In the framework of physical reservoir computing (RC), machine learning algorithms designed for digital computers are executed using analog computerlike nonlinear physical systems that can provide energy-efficient computational power for predicting time-dependent quantities that can be found using nonlinear differential equations. We suggest a bubble-based RC (BRC) system that combines the nonlinearity of an acoustic response of a cluster of oscillating gas bubbles in water with a standard echo state network (ESN) algorithm that is well suited to forecast chaotic time series. We confirm the plausibility of the BRC system by numerically demonstrating its ability to forecast certain chaotic time series similarly to or even more accurately than ESN.

Acoustic, Phononic, Brillouin Light Scattering and Faraday Wave-Based Frequency Combs: Physical Foundations and Applications.

Frequency combs (FCs)-spectra containing equidistant coherent peaks-have enabled researchers and engineers to measure the frequencies of complex signals with high precision, thereby revolutionising the areas of sensing, metrology and communications and also benefiting the fundamental science. Although mostly optical FCs have found widespread applications thus far, in general FCs can be generated using waves other than light. Here, we review and summarise recent achievements in the emergent field of acoustic frequency combs (AFCs), including phononic FCs and relevant acousto-optical, Brillouin light scattering and Faraday wave-based techniques that have enabled the development of phonon lasers, quantum computers and advanced vibration sensors. In particular, our discussion is centred around potential applications of AFCs in precision measurements in various physical, chemical and biological systems in conditions where using light, and hence optical FCs, faces technical and fundamental limitations, which is, for example, the case in underwater distance measurements and biomedical imaging applications. This review article will also be of interest to readers seeking a discussion of specific theoretical aspects of different classes of AFCs. To that end, we support the mainstream discussion by the results of our original analysis and numerical simulations that can be used to design the spectra of AFCs generated using oscillations of gas bubbles in liquids, vibrations of liquid drops and plasmonic enhancement of Brillouin light scattering in metal nanostructures. We also discuss the application of non-toxic room-temperature liquid-metal alloys in the field of AFC generation.

Spectrally wide acoustic frequency combs generated using oscillations of polydisperse gas bubble clusters in liquids.

Acoustic frequency combs leverage unique properties of the optical frequency comb technology in high-precision measurements and innovative sensing in optically inaccessible environments such as under water, under ground, or inside living organisms. Because acoustic combs with wide spectra would be required for many of these applications but techniques of their generation have not yet been developed, here we propose an approach to the creation of spectrally wide acoustic combs using oscillations of polydisperse gas bubble clusters in liquids. By means of numerical simulations, we demonstrate that clusters consisting of bubbles with precisely controlled sizes can produce wide acoustic spectra composed of equally spaced coherent peaks. We show that under typical experimental conditions, bubble clusters remain stable over time, which is required for a reliable recording of comb signals. We also demonstrate that the spectral composition of combs can be tuned by adjusting the number and size of bubbles in a cluster.

Acoustic frequency combs using gas bubble cluster oscillations in liquids: a proof of concept.

We propose a new approach to the generation of acoustic frequency combs (AFC)-signals with spectra containing equidistant coherent peaks. AFCs are essential for a number of sensing and measurement applications, where the established technology of optical frequency combs suffers from fundamental physical limitations. Our proof-of-principle experiments demonstrate that nonlinear oscillations of a gas bubble cluster in water insonated by a low-pressure single-frequency ultrasound wave produce signals with spectra consisting of equally spaced peaks originating from the interaction of the driving ultrasound wave with the response of the bubble cluster at its natural frequency. The so-generated AFC posses essential characteristics of optical frequency combs and thus, similar to their optical counterparts, can be used to measure various physical, chemical and biological quantities.

Excitation of Faraday-like body waves in vibrated living earthworms.

Biological cells and many living organisms are mostly made of liquids and therefore, by analogy with liquid drops, they should exhibit a range of fundamental nonlinear phenomena such as the onset of standing surface waves. Here, we test four common species of earthworm to demonstrate that vertical vibration of living worms lying horizontally on a flat solid surface results in the onset of subharmonic Faraday-like body waves, which is possible because earthworms have a hydrostatic skeleton with a flexible skin and a liquid-filled body cavity. Our findings are supported by theoretical analysis based on a model of parametrically excited vibrations in liquid-filled elastic cylinders using material parameters of the worm's body reported in the literature. The ability to excite nonlinear subharmonic body waves in a living organism could be used to probe, and potentially to control, important biophysical processes such as the propagation of nerve impulses, thereby opening up avenues for addressing biological questions of fundamental impact.

Beamed UV sonoluminescence by aspherical air bubble collapse near liquid-metal microparticles.

Irradiation with UV-C band ultraviolet light is one of the most commonly used ways of disinfecting water contaminated by pathogens such as bacteria and viruses. Sonoluminescence, the emission of light from acoustically-induced collapse of air bubbles in water, is an efficient means of generating UV-C light. However, because a spherical bubble collapsing in the bulk of water creates isotropic radiation, the generated UV-C light fluence is insufficient for disinfection. Here we show, based on detailed theoretical modelling and rigorous simulations, that it should be possible to create a UV light beam from aspherical air bubble collapse near a gallium-based liquid-metal microparticle. The beam is perpendicular to the metal surface and is caused by the interaction of sonoluminescence light with UV plasmon modes of the metal. We estimate that such beams can generate fluences exceeding 10 mJ/cm2, which is sufficient to irreversibly inactivate most common pathogens in water with the turbidity of more than 5 Nephelometric Turbidity Units.

Modal interferometric refractive index sensing in microstructured exposed core fibres.

Optical fibre-based sensors measuring refractive index shift in bodily fluids and tissues are versatile and accurate probes of physiological processes. Here, we suggest a refractive index sensor based on a microstructured exposed-core fibre (ECF). By considering a high refractive index coating of the exposed core, our modelling demonstrates the splitting of the guided mode into a surface sensing mode and a mode that is isolated from the surface. With the isolated mode acting as a reference arm, this two-mode one-fibre solution provides for robust interferometric sensing with a sensitivity of up to 60, 000 rad/RIU-cm, which is suitable for sensing subtle physiological processes within hard-to-reach places inside living organisms, such as the spinal cord, ovarian tract and blood vessels.

Harmonic and subharmonic waves on the surface of a vibrated liquid drop.

Liquid drops and vibrations are ubiquitous in both everyday life and technology, and their combination can often result in fascinating physical phenomena opening up intriguing opportunities for practical applications in biology, medicine, chemistry, and photonics. Here we study, theoretically and experimentally, the response of pancake-shaped liquid drops supported by a solid plate that vertically vibrates at a single, low acoustic range frequency. When the vibration amplitudes are small, the primary response of the drop is harmonic at the frequency of the vibration. However, as the amplitude increases, the half-frequency subharmonic Faraday waves are excited parametrically on the drop surface. We develop a simple hydrodynamic model of a one-dimensional liquid drop to analytically determine the amplitudes of the harmonic and the first superharmonic components of the linear response of the drop. In the nonlinear regime, our numerical analysis reveals an intriguing cascade of instabilities leading to the onset of subharmonic Faraday waves, their modulation instability, and chaotic regimes with broadband power spectra. We show that the nonlinear response is highly sensitive to the ratio of the drop size and Faraday wavelength. The primary bifurcation of the harmonic waves is shown to be dominated by a period-doubling bifurcation, when the drop height is comparable with the width of the viscous boundary layer. Experimental results conducted using low-viscosity ethanol and high-viscocity canola oil drops vibrated at 70Hz are in qualitative agreement with the predictions of our modeling.

Sensitive and Multiplexed SERS Nanotags for the Detection of Cytokines Secreted by Lymphoma.

The sensitive and simultaneous detection of cytokines will provide new insights into the physiological process and disease pathways due to the complex nature of cytokine networks. However, the key challenge is the lack of probes that can simultaneously detect multiple cytokines in a single sample. In this contribution, we proposed an alternative approach for sensitive cytokine detection in a multiplex manner by the use of a new set of surface-enhanced Raman spectroscopy (SERS) nanotags. Typically, the newly designed SERS nanotags are composed of gold nanoparticles as the core, tuneable Raman molecules as the reporters, and a thin silver layer as the shell. As demonstrated through rigorous numerical simulations, enhanced Raman signal is achieved due to a strong localization of light in the 0.2 nm thin, optically deep-subwavelength region between the Au core and the Ag shell. Sensitive detection of cytokines is realized by forming a sandwich immunoassay. The detection limit is down to 4.5 pg mL-1 (S/N = 3). The specificity of the assay is proved as negligible signals were detected for the false targets. Furthermore, multiple cytokines are simultaneously detected in a single assay from the secretion of B-lymphocyte cell line (Raji) after concanavalin A (Con A) stimulation. The results indicate that our method holds a significant potential for sensitive and multiplexed detection of cytokines and offers the opportunity for future applications in clinical settings.

UV plasmonic properties of colloidal liquid-metal eutectic gallium-indium alloy nanoparticles.

Nanoparticles made of non-noble metals such as gallium have recently attracted significant attention due to promising applications in UV plasmonics. To date, experiments have mostly focused on solid and liquid pure gallium particles immobilized on solid substrates. However, for many applications, colloidal liquid-metal nanoparticle solutions are vital. Here, we experimentally demonstrate strong UV plasmonic resonances of eutectic gallium-indium (EGaIn) liquid-metal alloy nanoparticles suspended in ethanol. We rationalise experimental results through a theoretical model based on Mie theory. Our results contribute to the understanding of UV plasmon resonances in colloidal liquid-metal EGaIn nanoparticle suspensions. They will also enable further research into emerging applications of UV plasmonics in biomedical imaging, sensing, stretchable electronics, photoacoustics, and electrochemistry.

Facile One-Pot Synthesis of Nanodot-Decorated Gold-Silver Alloy Nanoboxes for Single-Particle Surface-Enhanced Raman Scattering Activity.

Surface-enhanced Raman scattering (SERS) is an important, highly sensitive technique for chemical and biological analysis, which is critically dependent upon high-performance metallic substrates. Anisotropic gold (Au)-silver (Ag) alloy nanoboxes are attractive SERS substrates because of the greatly enhanced Raman signals from the strong electromagnetic fields on the sharp corners. Yet, the routine approach of Au-Ag alloy nanobox synthesis is still challenging because of the complicated procedures and use of biologically/environmentally unfriendly reagents. To facilitate the usage of Au-Ag alloy nanoboxes for broad SERS applications, we propose a facile green strategy to synthesize Au-Ag alloy nanoboxes with superior single-particle SERS sensitivity. Our novel straightforward strategy involves HAuCl4 and AgNO3 reduction by ascorbic acid, which is achieved in an aqueous one-pot reaction at ambient temperature. Significantly, the surfaces of the prepared Au-Ag alloy nanoboxes are judiciously designed to introduce nanodots, generating numerous "hot spots" for high Raman signal enhancement as indicated by rigorous numerical simulations. By combining scanning electron microscopy and Raman mapping images, we demonstrate the application of Au-Ag alloy nanoboxes for single-particle sensing SERS activity. The as-prepared Au-Ag alloy nanoboxes are expected to facilitate their further applications in quantitative and ultrasensitive SERS detection.

Extended depth of field imaging through multicore optical fibers.

Compact microendoscopes use multicore optical fibers (MOFs) to visualize hard-to-reach regions of the body. These devices typically have a large numerical aperture (NA) and are fixed-focus, leading to blurry images from a shallow depth of field with little focus control. In this work, we demonstrate a method to digitally adjust the collection aperture and therefore extend the depth of field of lensless MOF imaging probes. We show that the depth of field can be more than doubled for certain spatial frequencies, and observe a resolution enhancement of up to 78% at a distance of 50µm from the MOF facet. Our technique enables imaging of complex 3D objects at a comparable working distance to lensed MOFs, but without the requirement of lenses, scan units or transmission matrix calibration. Our approach is implemented in post processing and may be used to improve contrast in any microendoscopic probe utilizing a MOF and incoherent light.

Synthesis of discrete phase-coherent optical spectra from nonlinear ultrasound.

Nonlinear acoustic interactions in liquids are effectively stronger than nonlinear optical interactions in solids. Thus, harnessing these interactions will offer new possibilities in the design of ultra-compact nonlinear photonic devices. We theoretically demonstrate a new scheme for synthesis of optical spectra from nonlinear ultrasound harmonics using a hybrid liquid-state and nanoplasmonic device compatible with fibre-optic technology. The synthesised spectra consist of a set of equally spaced optical Brillouin light scattering modes having a well-defined phase relationship between each other. We suggest that these spectra may be employed as optical frequency combs whose spectral composition may be tuned by controlling the nonlinear acoustic interactions.

Plasmonic nanoantenna hydrophones.

Ultrasound is a valuable biomedical imaging modality and diagnostic tool. Here we theoretically demonstrate that a single dipole plasmonic nanoantenna can be used as an optical hydrophone for MHz-range ultrasound. The nanoantenna is tuned to operate on a high-order plasmon mode, which provides an increased sensitivity to ultrasound in contrast to the usual approach of using the fundamental dipolar plasmon resonance. Plasmonic nanoantenna hydrophones may be useful for ultrasonic imaging of biological cells, cancer tissues or small blood vessels, as well as for Brillouin spectroscopy at the nanoscale.

Magneto-Plasmonics and Resonant Interaction of Light with Dynamic Magnetisation in Metallic and All-Magneto-Dielectric Nanostructures.

A significant interest in combining plasmonics and magnetism at the nanoscale gains momentum in both photonics and magnetism sectors that are concerned with the resonant enhancement of light-magnetic-matter interaction in nanostructures. These efforts result in a considerable amount of literature, which is difficult to collect and digest in limited time. Furthermore, there is insufficient exchange of results between the two research sectors. Consequently, the goal of this review paper is to bridge this gap by presenting an overview of recent progress in the field of magneto-plasmonics from two different points of view: magneto-plasmonics, and magnonics and magnetisation dynamics. It is expected that this presentation style will make this review paper of particular interest to both general physical audience and specialists conducting research on photonics, plasmonics, Brillouin light scattering spectroscopy of magnetic nanostructures and magneto-optical Kerr effect magnetometry, as well as ultrafast all-optical and THz-wave excitation of spin waves. Moreover, readers interested in a new, rapidly emerging field of all-dielectric nanophotonics will find a section about all-magneto-dielectric nanostructures.

Transverse magneto-optical Kerr effect in subwavelength dielectric gratings.

We demonstrate theoretically a large transverse magneto-optical Kerr effect (TMOKE) in subwavelength gratings consisting of alternating magneto-insulating and nonmagnetic dielectric nanostripes. The reflectivity of the grating reaches 96% at the frequencies corresponding to the maximum of the TMOKE response. The combination of a large TMOKE response and high reflectivity is important for applications in 3D imaging, magneto-optical data storage, and magnonics.

Actively tunable bistable optical Yagi-Uda nanoantenna.

We propose and theoretically demonstrate a novel type of optical Yagi-Uda nanoantennas tunable via variation of the free-carrier density of a semiconductor disk placed in a gap of a metallic dipole feeding element. Unlike its narrowband all-metal counterparts, this nanoantenna exhibits a broadband unidirectional emission and demonstrates a bistable response in a preferential direction of the far-field zone, which opens up unique possibilities for ultrafast control of subwavelength light not attainable with dipole or bowtie architectures.

Inhibition, enhancement, and control of spontaneous emission in photonic nanowires.

We experimentally investigate the spontaneous emission (SE) rates of single InAs quantum dots embedded in GaAs photonic nanowires. For a diameter leading to the optimal confinement of the fundamental guided mode HE11, the coupling to HE11 dominates the SE process and an increase of the SE rate by a factor of 1.5 is achieved. When the diameter is decreased, the coupling to this mode vanishes rapidly, thus allowing the coupling to the other radiation modes to be probed. In these conditions, a SE inhibition factor of 16, equivalent to the one obtained in state-of-the-art photonic crystals, is measured. These results, which are supported by fully vectorial calculations, confirm the potential of photonic nanowires for a nearly perfect, broadband SE control.

Active control over nanofocusing with nanorod plasmonic antennas.

Active control over light nanofocusing in a nanorod plasmonic antenna coupled to a photonic crystal cavity is proposed and demonstrated by means of full-vectorial 3D simulations. By varying the excitation of the cavity with laser beam spot size allows us to achieve a gradual control over light nanofocusing at the tip of the nanoantenna. The demonstrated control mechanism eliminates the need for nonlinear effects or mechanical reconfiguration and represents a step towards the implementation of reliable tunable subwavelength light sources.