RESUMO
The relation was studied between the density of the electrical charge of the surface (DECS) and the karyological structure of the rat ovary tumour (OT) cells. For this purpose the sedimentation rate and electrical mobility of cells from two OT clones were measured. The OT1 and OT2 clones differed in their modal chromosome numbers (44 and 80-90, respectively). An increase in DECS by 4-5% on the average occurs in cells with the chromosome set close to the tetraploid one. The further increase in the chromosome number to the octaploid one does not change DECS. The results obtained show stability of DECS with significant changes in the structure of the OT cells karyotype.
Assuntos
Neoplasias Experimentais/genética , Animais , Células Clonais , Eletricidade , Cariotipagem , Neoplasias Experimentais/patologia , Ploidias , RatosRESUMO
Irradiation of rats with a dose of 7 Gy increases the density of electrical change of bone marrow cells by (8-15) X 10(3) elementary charge X micron-2 which makes up 12-26% of original density. The maximum effect was registered 30 min after irradiation. After 3 days the density of electrical charge decreased to reach the original values. In the peripheral blood erythrocytes, marked changes in the charge were only detected in 3 days when the charge decreased as compared to the control.
Assuntos
Medula Óssea/efeitos da radiação , Eletricidade , Lesões Experimentais por Radiação/patologia , Animais , Medula Óssea/patologia , Contagem de Células , Separação Celular , Sobrevivência Celular/efeitos da radiação , Eritrócitos/patologia , Eritrócitos/efeitos da radiação , Masculino , Ratos , Fatores de TempoRESUMO
The surface changes of the rat bone marrow cells was measured. For identification of cells, differences in sedimentational characteristics were used. With this purpose the velosity of sedimentation and electrophoretic mobility of cells were measured simultaneously. The decrease in the surface charge of myeloid cells was shown in the media with lowered ionic force.
