Cryptosporidium in fish: Implications for aquaculture and beyond.

Aquaculture industries are expanding worldwide and control of Cryptosporidium is of great importance. Cryptosporidiosis is a serious waterborne/foodborne disease, responsible for infectious outbreaks globally. Current knowledge on the Cryptosporidium species in the aquatic environment and their occurrence in piscine hosts is steadily increasing since the Cryptosporidium species have been detected in marine, freshwater, cultured, captive and ornamental fish in a wide range of geographical regions. The zoonotic potential of these parasites and their pathological impact on piscine hosts have been increasingly reported and the fishborne zoonotic risk from Cryptosporidium spp. is of major importance from a public health point of view. Zoonotic subtypes in fish have been described in various studies and are probably related to water contamination from animal and human wastes. This review critically evaluated existing scientific data, related to Cryptosporidium species in piscine hosts, emphasizing transmission routes and the potential impact of piscine cryptosporidiosis in aquaculture. This knowledge will facilitate consumers, authorities and water industries such as fisheries and aquaculture, the prevention and control of waterborne and fishborne cryptosporidiosis in fish products.

Whole brain transcriptomics of intermittently fed individuals of the marine teleost Sparus aurata.

A major challenge in fish physiology is to understand the mechanisms underlying the transcriptomic responses of fish brain to food deprivation. Differential gene expression analysis identified in total 2240 transcripts that presented >2-fold change (adjusted p < 0.01) between each treatment and the control group (C). The identity of the transcripts was obtained with annotation against multiple public databases and they were grouped according to their expression patterns. The gene ontology terms that were substantially affected were identified by functional annotation analysis. Genes related to ion transport, cell cycle and cell adhesion were mainly regulated during fasting and refeeding. These findings contribute to identify key indicators for the molecular basis of brain functions during periods of starvation in gilthead seabream.

Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq.

Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.

DNA damage and differential gene expression associated with physical stress in gilthead seabream (Sparus aurata).

Fish stress may result in inhibition of reproduction, development and growth. Thus, appropriate indices should be developed to accurately define the physiological plasticity of fish, in terms of coping with stress. Sea bream individuals were subjected to physical stress (fasting and confinement). DNA fragmentation of liver cells was assessed, in addition to gene expression of selected genes and plasma cortisol levels determination. Stress response was characterized with significant temporal alterations. Increased DNA fragmentation was observed as an aftereffect of physical stress and consequently gene expression of tp53 was stimulated. The expression pattern of glucocorticoid receptor (nr3c1) was directly correlated with plasma cortisol. Furthermore, glucokinase (gk) gene expression was considerably upregulated under acute stress, depicting putative energetic demands. Finally, igf1 downregulation during stress, reflects the suppression of the GH/IGF axis and the substantial stress effects on growth. To conclude, most of the indices described in the present study could be synergistically used, in order to robustly quantify physical stress in marine teleosts.