Cannabis sativa L. may reduce aggressive behaviour towards humans in shelter dogs.

Among the phytocomplex components of Cannabis sativa L., cannabidiol (CBD) has a recognised therapeutic effect on chronic pain. Little is known about the veterinary use of CBD in dogs. Even less is known on the effects of CBD on dog behaviour, especially in shelters. The purpose of this study was to determine if CBD affects stress related behaviour in shelter dogs. The sample consisted of 24 dogs divided into two groups that were created by assigning the dogs alternately: 12 dogs were assigned to the treatment group and 12 to the control group. Extra virgin olive oil, titrated to 5% in CBD was given to treated group; the placebo consisted of olive oil only, dispensed daily for 45 days. Behavioural data were collected using the 'focal animal' sampling method with 'all occurrences' and '1/0' methods for 3 h: before (T0), after 15 days (T1), after 45 days of treatment (T2) and after 15 days from the end of the treatment (T3). Treated dogs showed reduced aggressive behaviour toward humans following the treatment (Friedman Test: χ2 = 13.300; df = 3; N = 12; p = .004; adj. sig. p = 0.027), but the difference in the decrease of aggressive behaviour between the two groups was not significant (Mann-Whitney U test, T2-T0: Z = - 1.81; N = 24; p = 0.078). Other behaviours indicative of stress, such as displacing activities and stereotypes, did not decrease. Despite some non-significant results, our findings suggest that it is worth doing more research to further investigate the effect of CBD on dog behaviour; this would be certainly valuable because the potential for improving the welfare of dogs in shelters is priceless.

Gastrointestinal parasites in shelter cats of central Italy.

In Italy, the limited resources and the large number of cats living in feline shelters may facilitate the spread of parasite infections that may affect the health of cats and expose shelter workers and adoptive owners to zoonosis. Prevalence and risk factors of potential zoonotic and host-specific gastrointestinal parasite infections were assessed in cats living in public and private shelters of central Italy. Individual fecal samples were examined macroscopically and then screened microscopically by fresh and Lugol stained fecal smears and by flotation and the Baermann tests. A commercial rapid immune-chromatographic assay was used to detect Giardia duodenalis and Cryptosporidium spp. All animals were clinically examined to evaluate presence of clinical signs compatible to parasitic infections. Data were statistically analysed by multivariate analysis with logistic regression and chi square testing for bivariate analysis. The overall prevalence of gastrointestinal parasites in the shelter cats surveyed was 22% (29/132), with 26/132 (19.7%) of the animals infected with potentially zoonotic organisms. Upon statistical analysis, parasite infections were identified in significantly more cats younger than 18 months of age (p <0.05), and most often associated with the presence of compatible clinical signs (p <0.05). A higher prevalence of protozoan infections (18.1%, 24/132) than of helminth infections (12.9%, 17/132) was observed. Identified parasites were Giardia duodenalis (10.6%, 14/132), Toxocara cati (9%, 12/132), Cystoisospora felis (3%, 4/132), Cystoisospora rivolta (2.3%, 3/132), hookworms (2.3%, 3/132), Cryptosporidium spp. (1.6%, 2/132), Aonchotheca putorii (0.75%, 1/132), Tritrichomonas foetus (0.75%, 1/132) and Strongyloides sp. (0.75%, 1/132). Co-infections were identified in the 7.6% of animals surveyed. Results obtained from this study indicate high gastrointestinal parasite risk to shelter cats and public health in the examined areas and suggest the need for more effective control measures in shelters surveyed.

Evaluation of Unowned Domestic Cat Management in the Urban Environment of Rome After 30 Years of Implementation of the No-Kill Policy (National and Regional Laws).

Law no. 281, enacted by the Italian Parliament in 1991, was the first that aimed at managing urban free-roaming cats living in colonies, without killing and/or moving them from their site. It had been anticipated by the Lazio Regional Law no. 63/1988 and subsequently refined by the Lazio Regional Law no. 34/1997. These laws introduced: (i) the cats' right to live free and safe; (ii) the compulsory neutering of cats by the Veterinary Services of the Local Health Unit; (iii) the institutionalization of cat caretakers. Within this context, this paper intends to evaluate the effects of the application of the Italian laws on management of urban free-roaming cats for the years 1988 to 2018. To this end, some indicators have been built and applied to our activity data: number of censused colonies and number of cats; number of stable colonies due to neutering; number of hygiene and sanitary notifications; number of notifications to check cat welfare; number of bites by unowned free-roaming cats; number of notifications of cat poisoning. The number of citizens' requests for institutional interventions by public veterinary services in cat colonies management and, accordingly, the detection of cat colonies yet unknown, seem to confirm the interest of people to control the cat colonies in Rome in a humanitarian way, as evidenced in our data. This fact/phenomenon should be analyzed in its multiple dimensions, also including the many changes and social unrests which have affected the human-cat relationship in the last 30 years.

Leishmania infantum, Dirofilaria spp. and other endoparasite infections in kennel dogs in central Italy.

Prevalence and risk factors of Leishmania infantum, Dirofilaria spp. and other potentially zoonotic or canine-specific endoparasite infections were assessed in 639 kennel dogs from central Italy. To this end, individual blood and fecal samples were examined using parasitological, immunological and molecular techniques. The presence of compatible clinical pictures, as well as age and gender were considered as putative risks factors. To evaluate risk factors, multivariable analysis with logistic regression and univariable analysis with a Chi square test and a Fischer's exact test were performed. Overall, 52.6% of dogs (95% CI 48.6-56.5) were found positive, while 39.6% of dogs (95% CI 35.8-43.5) were infected by potentially zoonotic species. Leishmania infantum and Dirofilaria repens showed prevalences of 2.5% (95% CI 1.5-4.1) and 2.8% (95% CI 1.7-4.5), respectively. The prevalence of cardiorespiratory parasites was 7.8% (95% CI 5.9-10.3) and included the species Angiostrongylus vasorum, Eucoleus aerophilus, Eucoleus boehmi and D. immitis; the latter showed a prevalence of 0.2% (95% CI 0.001-1). Intestinal parasites were significantly prevalent (38.8%, 95% CI 35-42.7) and they consisted mainly of species of major zoonotic concern, including ancylostomatids, Toxocara canis, Giardia duodenalis, Dipylidium caninum, Taeniidae, Strongyloides stercoralis and Cryptosporidium parvum. Endoparasites were significantly prevalent in clinically suspected dogs. Leishmania infantum and cardiorespiratory nematodes were prevalent in older dogs, while intestinal parasites were prevalent in younger dogs. Results show high dog and public health risks in kennels in central Italy, and suggest the need for more effective control measures.