Assuntos
Cromossomos , Suínos/genética , Animais , Mapeamento Cromossômico , Proteínas de Ligação a DNA/genética , Células Híbridas , Vison , Dados de Sequência Molecular , Neurofibromatose 1/genética , Reação em Cadeia da Polimerase , Receptor Tipo 4 de Fator de Crescimento de Fibroblastos , Receptores de Fatores de Crescimento de Fibroblastos/genética , Receptores de Mineralocorticoides/genéticaAssuntos
Repetições de Dinucleotídeos/genética , Vison/genética , Polimorfismo Genético/genética , Alelos , Animais , Mapeamento Cromossômico/veterinária , Cosmídeos/química , Primers do DNA/química , Marcadores Genéticos , Hibridização in Situ Fluorescente/veterinária , Masculino , Reação em Cadeia da Polimerase/veterináriaRESUMO
This report extends the genetic map of the common shrew (Sorex araneus) by use of a clone panel of shrew-Chinese hamster and shrew-mouse hybrid cells (Pack et al., 1995; Matyakhina et al., 1996). This set of hybrid clones made it possible to assign the shrew genes for isocitrate dehydrogenase 2 (IDH2), inorganic pyrophosphatase (PP), glutamicpyruvate transaminase (GPT), adenosine kinase (ADK), glucuronidase 2 (GUSB) and acid phosphatase 2 (ACP2) to chromosome ik; the genes for adenylate kinases 1 and 3 (AK1 and AK3) to chromosome af; the genes for glutamate-oxaloacetate transaminase 2 (GOT2), peptidase D (PEPD) and growth hormone (GH) to chromosome hn; the gene for phosphoglucomutase 2 (PGM2) to chromosome go, the gene for enolase 1 (ENO1) to chromosome ji, the gene for ornithine carbamoyl-transferase (OTC) to chromosome de, the gene for aminoacylase 1 (ACY1) to arm m (chromosome mp), the gene for glutamate-oxaloacetate transaminase 1 (GOT1) to arm q (chromosome qr). Thus, the genetic map of the common shrew now contains 33 genes and it is possible to compare the syntenic associations with other species.
Assuntos
Mapeamento Cromossômico , Musaranhos/genética , Animais , Cricetinae , Cricetulus , DNA/genética , Enzimas/genética , Marcadores Genéticos , Células Híbridas , CamundongosAssuntos
Mapeamento Cromossômico , Raposas/genética , Hormônio do Crescimento/genética , Ornitina Carbamoiltransferase/genética , Príons/genética , Somatostatina/genética , Animais , Cricetinae , Cricetulus , Humanos , Células Híbridas , Camundongos , Ratos , Homologia de Sequência do Ácido NucleicoRESUMO
The chromosome localization of the gene encoding growth hormone (GH) was determined by Southern blotting of DNA obtained from a panel of common shrew x Chinese hamster and common shrew x mouse hybrid somatic cell clones using mink GH DNA as a probe. The GH gene was found to be localized on chromosome hn of the common shrew.
Assuntos
Hormônio do Crescimento/genética , Musaranhos/genética , Animais , Southern Blotting , Mapeamento Cromossômico , Cricetinae , Ligação Genética , Células Híbridas , Especificidade da EspécieRESUMO
Chromosomal localization of the genes for gamma- and kappa-immunoglobulins (IGGC and IGKC, respectively), aldolase B (ALDB), prion protein (PRNP), homeo box B (HOXB), and glutamate pyruvate transaminase (GPT) were determined with the use of mink-rodent hybrid cells. Analysis of segregation of the mink markers and chromosomes in these hybrid cells allowed us to assign the gene for HOXB to Chromosome (Chr) 8, IGGC to Chr 10, PRNP and IGKC to Chr 11, ALDB to Chr 12, and GPT to Chr 14 in mink. Furthermore, using a set of mink-mouse hybrid cells carrying fragments of mink Chr 8 of different sizes, we assigned the gene for HOXB to the pter-p26 region of the short arm of Chr 8. Comparative mapping of the genes of mink, human, and mouse, as well as other mammalian species, demonstrated that the mink genes HOXB, PRNP, ALDB, and IGGC are members of a conserved region shared by many mammalian species in common; the IGKC gene is a member of a conserved region common to carnivores and primates, not rodents; the GPT gene is a member of a syntenic gene group probably unique to the Mustelidae family or carnivores.
