Nitrogen efficiency in marandupalisadegrass pastures under increasing nitrogen levels / Eficiência do nitrogênio em pastagens de capim-marandu adubadas com doses crescentes de nitrogênio

The use of nitrogen (N) in pastoral ecosystems leads to increased productivity, as it allows the plant to elongate its leaves and, therefore, grazing herbivores harvest the green leaves. However, there are very volatile N sources, which can be replaced by ammonium nitrate, which is less volatile and less dependent on the application in rainy days. The treatments are compound of Marandu palisade grass pastures managed under continuous stocking at a canopy height of 25 cm, with different levels of N fertilizer: 0, 75, and 150 kg ha-1year-1, as ammonium nitrate (32% of N), with four replicates (pastures) in a completely randomized design. Nitrogen uptake (54.9, 96.5, 113.8 kg N ha-1) and N nutrition index (0.67, 0.98, 1.15) were different between N level, respectively, 0, 75 and 150 kg ha-1 year-1. The N recovery (58.3, 40.9 %) differed between 75 and 150 kg ha-1 year-1, respectively. The dose of 75 kg N kg ha-1 year-1 results in better N utilization, while the dose of 150 kg N ha-1 year-1 enables greater stocking rate; therefore, requiring less grazing area.

O uso de nitrogênio (N) em ecossistemas pastoris leva ao aumento da produtividade, pois permite que a planta alongue suas folhas e, portanto, os herbívoros colham as folhas verdes. Porém, existem fontes de nitrogênio muito voláteis, que devem ser substituídas por nitrato de amônio, que é menos volátil e menos dependente da aplicação em dias chuvosos. Os tratamentos foram compostos por pastagens de capim-marandu, manejadas sob lotação contínua, na altura do dossel a 25 cm, com diferentes doses de N: 0, 75 e 150 kg ha-1 ano-1, na forma de nitrato de amônio (32% de N), com quatro repetições (piquetes), em delineamento inteiramente casualizado. A absorção de nitrogênio (54,9; 96,5; 113,8 kg N ha-1) e o índice nutricional de N (0,67; 0,98; 1,15) foram diferentes entre as doses de N, respectivamente, 0, 75 e 150 kg ha-1 ano-1. A recuperação de N (58,3; 40,9%) diferiu entre 75 e 150 kg ha-1 ano-1, respectivamente. A dose de 75 kg N kg ha-1 ano-1 resultou em melhor aproveitamento do N, enquanto a dose de 150 kg N ha-1 ano-1 possibilita maior taxa de lotação; portanto, exigindo menos área de pastagem.

N-fertilization of tropical pastures improves performance but not methane emission of Nellore growing bulls.

Grazing management and N-fertilizer have been reported to improve tropical forage productivity and quality, however, their effect on methane emission of grazing animals remains uncertain. Therefore, this study aimed to assess the effects of increasing application rates of nitrogen (N) fertilization of Marandu palisadegrass under continuous stocking on intake, digestibility, nitrogen balance, and enteric methane emissions of Nellore growing bulls. We hypothesized that changes in the forage nutritive value caused by N fertilization of pastures combined with adequate grazing management (e.g., greater crude protein [CP] and digestibility) would lead to an increase in animal productivity (e.g., greater average daily gain [ADG] and gain per area), and then, to a decrease in methane emission intensity. Treatments consisted of different annual application rates of nitrogen fertilization: 0, 75, and 150 kg N/ha using ammonium nitrate (32% N) as the nitrogen source. The experimental design was completely randomized, with three treatments and four replications (12 paddocks). Intake, digestibility, N balance, and methane emissions were measured in eight animals per treatment. CP intake, digestibility and N balance increased linearly with the increase in N fertilization (P < 0.05). In addition, stocking rate (SR) and ADG linearly increased from 1.75 animal unit (AU = 450 kg)/ha and 0.62 kg/d (0 kg N/ha) to 3.75 AU/ha and 0.82 kg/d (150 kg N/ha), respectively. Individual methane emissions nor methane emission intensity were affected by treatment with an average of 164.7 g/d and 199.7 g/kg ADG (P > 0.05). Annual N fertilization with ammonium nitrate between 75 and 150 kg N/ha in palisadegrass pastures under continuous stocking enhances animal performance per unit area yet not affecting neither methane production nor intensity.

The availability of nitrogen in the soil is one of the main factors that can affect plant growth and characteristics. Nitrogen fertilization is a tool to increase the efficiency in the productive responses of grazing beef cattle, reconciling the greater production per area, reduction of the production cycle, maximization of the use of nutrients, with the maintenance of the system sustainability by enhancing animal production indexes. The present study evaluated performance and methane production of Nellore growing bulls grazing continuously stocked Marandu palisadegrass fertilized with three rates of ammonium nitrate (0, 75, and 150 kg N/ha). Although nitrogen fertilization did not affect individual methane production, both stocking rate and average daily gain linearly increased as fertilization rate increased, thus increasing the beef productivity.

Effects of rumen undegradable protein sources on nitrous oxide, methane and ammonia emission from the manure of feedlot-finished cattle.

The effects of sources of rumen undegradable protein (RUP) in diets on methane (CH4), nitrous oxide (N2O) and ammonia (NH3) emissions from the manure of feedlot-finished cattle were evaluated. We hypothesized that the use of different RUP sources in diets would reduce N loss via urine and contribute to reduced N2O, CH4 and NH3 emissions to the environment. Nellore cattle received different diets (18 animals/treatment), including soybean meal (SM, RDP source), by-pass soybean meal (BSM, RUP source) and corn gluten meal (CGM, RUP source). The protein source did not affect the N and C concentration in urine, C concentration in feces, and N balance (P > 0.05). The RUP sources resulted in a higher N2O emission than the RDP source (P = 0.030), while BSM resulted in a higher N2O emission than CGM (P = 0.038) (SM = 633, BSM = 2521, and CGM = 1153 g ha-2 N-N2O); however, there were no differences in CH4 and NH3 emission (P > 0.05). In conclusion, the use of RUP in diets did not affect N excretion of beef cattle or CH4 and NH3 emission from manure, but increased N2O emission from the manure.

Arginine requirement for egg production in Japanese quail.

The objective of this study was to determine the ideal arginine intake for egg production in Japanese quail using the dilution technique. A completely random design was used, with 8 treatments (seven concentrations of arginine plus a control diet) and ten replicates, totaling 80 Japanese quails. The digestible arginine levels included in the study were 0.361%, 0.603%, 0.843%, 1.084%, 1.204%, 1.311%, and 1.460%. The variables analyzed were feed intake, egg production (EP), egg weight, egg output (EO), feed conversion ratio, and body weight were performed using a mixed model. When the effect of arginine levels (P ≤ 0.05) was detected, the model's broken line linear-plateau (BL), quadratic-plateau (BLq), and the first intercept of the BLq in the plateau of BL were adjusted to determine the ideal arginine intake. It observed that the arginine levels modified the quail responses (P < 0.001). Egg production was 10% with the 0.361% arginine in diet and recovered (97%) with the 1.311% arginine diet. The BL and BLq models estimated 232 mg/quail per day and 351 mg/quail per day for EO, respectively. The first intercept obtained was 290 mg/quail per day or 1,411%, which was considered the optimum level arginine intake for EO in Japanese quail.

Meat quality of young bulls finished in a feedlot and supplemented with clove or cinnamon essential oils.

Forty young crossbred bulls (10 ± 2.2 months of age; 219 ± 11.7 kg) were finished in a feedlot with one of five different treatments: CON, control (no essential oil); CLO450, clove essential oil (450 mg/kg); CLO880, clove essential oil (880 mg/kg); CIN450; cinnamon essential oil (450 mg/kg); and CIN880, cinnamon essential oil (880 mg/kg). Instrumental meat quality attributes and consumer acceptability aspects (visual and sensory) were assessed during meat ageing and display. The inclusion of clove essential oil showed (P = 0.033) a quadratic effect on lipid oxidation in meat evaluated under display conditions. Level of cinnamon essential oil affected (P < 0.05) pH, shear force, and meat colour. On the other hand, the diet had no effect (P > 0.05) on sensory or visual acceptability. Ageing (7 days) enhanced (P < 0.05) sensory acceptability attributes. Globally, the addition of essential oil in bull diets can be an alternative to reduces lipid oxidation without modifying sensory acceptability attributes.

Effect of pasture management on enteric methane emissions from goats.

The effect of pasture management on CH4 emissions was investigated from goats in a tropical climate. Two experiments were conducted in a "Tanzania Guinea grass" (Panicum maximum Jacq.) pasture to assess enteric CH4 production in a completely randomized design. Emissions from light, moderate, and heavy grazing intensities were analyzed in the first experiment, and variations between grazing days were explored in the second experiment. Grazing intensity was defined as 2.4, 1.6, and 0.8 post-grazing leaf area index. Pasture management employed intermittent grazing with variable stocking rate using Anglo Nubian female adult goats. SF6 tracer gas technique was used to measure CH4 production. Grazing intensity was not found to affect CH4 emissions per animal, dry matter forage intake (DMI), and gross energy (GE) intake. However, the second experiment showed that CH4 production was influenced by the grazing day. CH4 emissions were 18.1 g day-1, and the variables were 0.88 g kg-1 of metabolic weight, 17.45 g kg-1 of DMI, and 5.5% of GE. CH4 production increased linearly with the grazing day, possibly reflecting a reduction in forage quality. These findings suggest that the day of occupation in intermittent grazing has a greater effect on CH4 emissions than that by grazing intensity and that a single day grazing of Tanzania Guinea grass could mitigate CH4 emissions.

Mixed nonlinear models in ruminal in situ degradability trials / Modelos não lineares mistos em ensaios de degradabilidade ruminal in situ

Classical methods of analysis of nonlinear models are widely used in studies of ruminal degradation kinetics. As this type of study involves repeated measurements in the same experimental unit, the use of mixed nonlinear models (MNLM) is proposed, in order to solve problems of heterogeneity of variances of the responses, correlation among repeated measurements and consequent lack of sphericity in the covariance matrix. The aims of this work are to present an evaluation of the applicability of MNLM in the estimation of parameters to describe the in situ ruminal degradation kinetics of the dry matter of Tifton 85 hay and to compare the results with those obtained from the usual analysis in two-phases. The steers used in the trial were fed diets composed of three different combinations of roughage and concentrate and two hays with different nutritional qualities. The proposed approach was proven as effective as the traditional one for estimating model parameters. However, it adequately models the correlation among the longitudinal data, which can affect the estimates obtained, the standard error associated with them and potentially change the results of the inferences. It is quite attractive when the research seeks to understand the behavior of the process of food degradation throughout the incubation times.(AU)

Métodos clássicos de análise de modelos não lineares são amplamente utilizados em estudos da cinética de degradação ruminal. Como esse tipo de estudo envolve medidas repetidas na mesma unidade experimental, propõe-se o uso de modelos não lineares mistos (MNLM), buscando resolver os problemas de heterogeneidade de variâncias das respostas, de correlação entre as medidas repetida se a consequente falta de esfericidade da matriz de covariâncias. Os objetivos deste trabalho envolvem apresentar uma avaliação da aplicabilidade dos MNLM na estimação de parâmetros para descrever a cinética de degradação ruminal in situ da matéria seca de fenos de capim-Tifton 85 e comparar os seus resultados com os obtidos da análise usual realizada em duas fases. Os novilhos utilizados no ensaio foram alimentados com rações compostas por três diferentes combinações de volumoso e concentrado e dois fenos com diferentes qualidades nutricionais. A abordagem proposta mostrou-se tão efetiva quanto à tradicional para a estimação dos parâmetros do modelo. Contudo, ela modela de forma adequada a correlação entre os dados longitudinais, o que pode afetar as estimativas obtidas, o erro padrão associado a elas e, potencialmente, alterar os resultados das inferências. É bastante atraente quando a pesquisa busca entender o comportamento do processo da degradação dos alimentos ao longo dos tempos de incubação.(AU)

Economic evaluation from beef cattle production industry with intensification in Brazil's tropical pastures.

This study aimed to evaluate different scenarios (year, supplementation level) about economic results of beef cattle production during rearing and finishing phase in Brazilian's tropical pastures. Four scenarios were evaluated in combination with fourteen supplements, and it was originated from some research developed inside Forage Crops and Grasslands section from São Paulo State University among years 2011 and 2014. The economic evaluation was analyzed by operating cost, total operational costs, gross revenue, operating profit, and financial net income. Besides profitability, internal rate of return (IRR), benefit/cost ratio (B:C), and simple payback period (SPP) were calculated too. During rearing phase, the best result was observed for scenario 2 (2012), supplement 3.2 (mineral mix) with values of 11 cycles, 26.3%, 9.30%, and 0.39 for SPP, profitability, IRR, and B:C ratio, respectively. Already to finishing phase, the best scenario was 3 (2013), supplement 10 (multiple supplement with supplementation level equal 1.0% body weight), which obtained 4 cycles, 68.7%, 27.00%, and 2.34 for the same variables above mentioned. Results were consistent being that higher IRR and profitability occurred when using low supplementation level. Hence, the economic responses from different scenarios (years and supplements) can alter the final livestock farm financial statement.

Mixed nonlinear models in ruminal in situ degradability trials / Modelos não lineares mistos em ensaios de degradabilidade ruminal in situ

Classical methods of analysis of nonlinear models are widely used in studies of ruminal degradation kinetics. As this type of study involves repeated measurements in the same experimental unit, the use of mixed nonlinear models (MNLM) is proposed, in order to solve problems of heterogeneity of variances of the responses, correlation among repeated measurements and consequent lack of sphericity in the covariance matrix. The aims of this work are to present an evaluation of the applicability of MNLM in the estimation of parameters to describe the in situ ruminal degradation kinetics of the dry matter of Tifton 85 hay and to compare the results with those obtained from the usual analysis in two-phases. The steers used in the trial were fed diets composed of three different combinations of roughage and concentrate and two hays with different nutritional qualities. The proposed approach was proven as effective as the traditional one for estimating model parameters. However, it adequately models the correlation among the longitudinal data, which can affect the estimates obtained, the standard error associated with them and potentially change the results of the inferences. It is quite attractive when the research seeks to understand the behavior of the process of food degradation throughout the incubation times.

Métodos clássicos de análise de modelos não lineares são amplamente utilizados em estudos da cinética de degradação ruminal. Como esse tipo de estudo envolve medidas repetidas na mesma unidade experimental, propõe-se o uso de modelos não lineares mistos (MNLM), buscando resolver os problemas de heterogeneidade de variâncias das respostas, de correlação entre as medidas repetida se a consequente falta de esfericidade da matriz de covariâncias. Os objetivos deste trabalho envolvem apresentar uma avaliação da aplicabilidade dos MNLM na estimação de parâmetros para descrever a cinética de degradação ruminal in situ da matéria seca de fenos de capim-Tifton 85 e comparar os seus resultados com os obtidos da análise usual realizada em duas fases. Os novilhos utilizados no ensaio foram alimentados com rações compostas por três diferentes combinações de volumoso e concentrado e dois fenos com diferentes qualidades nutricionais. A abordagem proposta mostrou-se tão efetiva quanto à tradicional para a estimação dos parâmetros do modelo. Contudo, ela modela de forma adequada a correlação entre os dados longitudinais, o que pode afetar as estimativas obtidas, o erro padrão associado a elas e, potencialmente, alterar os resultados das inferências. É bastante atraente quando a pesquisa busca entender o comportamento do processo da degradação dos alimentos ao longo dos tempos de incubação.

Does virginiamycin supplementation affect the metabolism and performance of Nellore bulls grazing under low and high gain rates?

This study aimed to evaluate the effect of virginiamycin on the metabolism and performance of growing Nellore bulls under low and high gain rates on pasture. In experiment 1, 80 Nellore bulls (age = 12 ± 2 months, body weight = 258 ± 15 kg) were assigned to 16 paddocks in a 2 × 2 randomized block factorial arrangement. In experiment 2, 12 cannulated Nellore bulls were assigned to three 4 × 4 balanced Latin squares. The factors were: (1) mineral salt without or with virginiamycin, and (2) low or high gain rate. No interaction was noted between factors (p > .10). Animals fed virginiamycin had greater average daily gain (14%, p < .01), body weight (11 kg, p = .05), plasma nonesterified fatty acid (20%, p < .01), serum calcium concentration (2.62%, p = .04), and total protozoa (p = .03) and had the same bacterial proportion (p > .27). Animals with a low gain rate had greater serum urea concentration (19.6%, p < .01) and ruminal ammonia nitrogen (62%, p < .01). Thus, virginiamycin increases the performance and changes the metabolism of growing Nellore bulls under low and high gain rates on pasture.

Comparison of laboratory methods to assess fiber contents in feedstuffs / Comparación de métodos de laboratorio para determinar el contenido de fibra en alimentos para animales / Comparação de metodologias laboratoriais para determinação de fibras em alimentos

Summary Background: the conventional method of fiber analysis using neutral detergent fiber (NDF) and acid detergent fiber (ADF) was proposed by Peter Van Soest. The main advantage of this method is the precision of results; however, reagents are costly and laboratory work requires long runtime and labor. Objective: to compare analytical data from different methodologies used to assess NDF and ADF contents. Methods: means obtained with the conventional method were compared through Dunnett's test (α=5%) with values from alternative methods using autoclave as the digester system. A completely randomized design in a 4 x 2 factorial arrangement was used. Results: NDF content through alternative methods was assessed for Tifton 85 hay, babassu meal and sugarcane, whereas ADF was only determined for babassu meal. NDF and ADF did not differ (p(0.05) between non-sequential or sequential analysis for all the feedstuffs and methods studied, except for ADF in corn silage. Conclusion: alternative methodologies allow reducing operating costs and time but lack uniformity and accuracy for analyzing ADF in corn silage.

Resumen Antecedentes: la determinación de los valores de fibra detergente neutra (FDN) y fibra detergente ácida (FDA) es de gran importancia en la nutrición animal, siendo el método tradicional de análisis el propuesto por Van Soest. Este método presenta como ventaja principal la precisión de los resultados, sin embargo la rutina de laboratorio en su ejecución implica mucho tiempo debido a sus etapas manuales, además de ser un método de alto costo que genera considerables proporciones de residuos químicos. Objetivo: evaluar diferentes metodologías propuestas para la análisis de las FDN y FDA mediante la medición de los datos analíticos. Métodos: las medias obtenidas por el método tradicional fueron comparadas con las medias de métodos alternativos que usan una autoclave como sistema digestor por el test de Dunnette (α=5%). Un diseño completamente aleatorio, en un diseño factorial 4x2 fue adoptado. Resultados: los métodos alternativos fueron recomendadas para las determinaciones de FDN por el heno de Tifton 85, salvado de harina de babasú y caña de azúcar, mientras que las determinaciones de los valores de FDA apenas para el salvado de harina de babasú. Los resultados de los análisis de FDN y FDA no fueron significativamente diferentes (p(0.05) entre los análisis no secuencial y secuencial, en todos los alimentos y métodos estudiados, a excepción de la determinación de FDA en el ensilaje de maíz. Conclusión: las metodologías alternativas permitieron ganancias significativas con la reducción de costos operacionales y del tiempo de análisis, pero sin uniformidad en la precisión de los análisis involucrados.

Resumo Antecedentes: as determinações dos teores de fibra em detergente neutro (FDN) e em fibra em detergente ácido (FDA) são de extrema importância na nutrição animal, tendo como método convencional de análise o proposto por Van Soest. Esse método apresenta como principal vantagem a precisão dos resultados, no entanto, a rotina laboratorial da sua execução demanda muito tempo por possuir etapas manuais, além do que é um método de custo elevado e que gera resíduos químicos em proporções consideráveis. Objetivo: avaliar diferentes metodologias propostas para análise da FDN e FDA, através da verificação dos dados analíticos. Métodos: as médias obtidas pelo método convencional foram comparadas com as médias de métodos alternativos que utilizam a autoclave como sistema digestor, pelo teste de Dunnette (α=5%). O delineamento adotado foi o inteiramente casualizado, em um esquema fatorial 4x2. Resultados: os métodos alternativos foram recomendados para determinação de FDN no feno de Tifton 85, farelo de babaçu e cana-de-açúcar, enquanto para as determinações dos teores de FDA, apenas no farelo de babaçu. A precisão observada nas análises dos teores de FDN e FDA não diferiram significativamente (p(0.05) quanto a análise não sequencial e sequencial, em todos os alimentos e métodos estudados, com exceção da determinação de FDA na silagem de milho. Conclusão: as metodologias alternativas permitiram ganhos significativos com a redução dos custos operacionais e do tempo da análise, mas sem uniformidade na precisão das análises desenvolvidas.

Effects of postgrazing residue on morphogenetic and structural characteristics of Tifton 85 pastures / Efeitos de resíduos pós-pastejo sobre características morfogênicas e estruturais de pastos de Tifton 85

The ideal time to start grazing is when pastures reach 95% light interception. The use of residual leaf area index (rLAI) to time the interruption of grazing under intermittent grazing has recently been studied in forage species in different climatic conditions in Brazil. However, studies evaluating the formation and development of leaves and tillers through morphogenetic and structural variables in functional rLAI are still lacking for Tifton 85 pastures. Therefore, the objective of this study was to evaluate the effects of varying rLAI in successive grazing cycles on morphogenetic and structural characteristics of Tifton 85 pasture grazed intermittently by sheep. Morphogenetic and structural characteristics were evaluated in three grazing cycles under three rLAI conditions (0.8, 1.6, and 2.4). The regrowth interval (time taken to return to 95% light interception) of pastures increased from 21.33 to 29.66 days with decreasing rLAI. The leaf appearance rate increased at a rate of 0.02 leaves tiller-1 day-1 and the number of live leaves per tiller increased by 0.41 with decreasing rLAI. Phyllochron, leaf elongation rate, final leaf length, leaf senescence rate, and stem elongation rate decreased linearly with decreasing rLAI. The number of live leaves per tiller was lower (7.97) in the third grazing cycle, probably owing to the higher senescence rate observed that same cycle, wh

O momento de iniciar o pastejo quando os pastos atingem 95% de interceptação luminosa e de interrupção do pastejo por meio de índices de área foliar residual (IAFr), em situação de pastejo intermitente, recentemente vem sendo estudado em espécies forrageiras em distintas condições climáticas do Brasil, porém, pesquisas avaliando a formação e desenvolvimento de folhas e perfilhos por meio das variáveis morfogênicas e estruturais em função de IAFr são ainda inexistentes em pastos de Tifton 85. Por isso, objetivou-se com este trabalho, avaliar os efeitos de IAFr dos pastos, em ciclos de pastejos sucessivos, sobre as características morfogênicas e estruturais de Tifton 85 pastejados de maneira intermitente por ovinos. Características morfogênicas e estruturais dos pastos foram avaliadas em três ciclos de pastejo em função de três IAFr (0,8; 1,6 e 2,4). O intervalo de rebrotação dos pastos aumentou de 21,33 para 29,66 dias com a diminuição do IAFr. A taxa de aparecimento de folhas aumentou 0,02 folhas perfilho-1dia-1 e o número de folhas vivas por perfilho aumentou 0,41 com a diminuição do IAFr. Filocrono, taxa de alongamento de folhas, comprimento final de folha, taxa de senescência foliar e taxa de alongamento do colmo decresceram linearmente com a diminuição do IAFr dos pastos. Somente o número de folhas vivas por perfilho foi menor (7,97) no terceiro ciclo de pastejo, provavelme

Effects of postgrazing residue on morphogenetic and structural characteristics of Tifton 85 pastures / Efeitos de resíduos pós-pastejo sobre características morfogênicas e estruturais de pastos de Tifton 85

The ideal time to start grazing is when pastures reach 95% light interception. The use of residual leaf area index (rLAI) to time the interruption of grazing under intermittent grazing has recently been studied in forage species in different climatic conditions in Brazil. However, studies evaluating the formation and development of leaves and tillers through morphogenetic and structural variables in functional rLAI are still lacking for Tifton 85 pastures. Therefore, the objective of this study was to evaluate the effects of varying rLAI in successive grazing cycles on morphogenetic and structural characteristics of Tifton 85 pasture grazed intermittently by sheep. Morphogenetic and structural characteristics were evaluated in three grazing cycles under three rLAI conditions (0.8, 1.6, and 2.4). The regrowth interval (time taken to return to 95% light interception) of pastures increased from 21.33 to 29.66 days with decreasing rLAI. The leaf appearance rate increased at a rate of 0.02 leaves tiller-1 day-1 and the number of live leaves per tiller increased by 0.41 with decreasing rLAI. Phyllochron, leaf elongation rate, final leaf length, leaf senescence rate, and stem elongation rate decreased linearly with decreasing rLAI. The number of live leaves per tiller was lower (7.97) in the third grazing cycle, probably owing to the higher senescence rate observed that same cycle, wh

O momento de iniciar o pastejo quando os pastos atingem 95% de interceptação luminosa e de interrupção do pastejo por meio de índices de área foliar residual (IAFr), em situação de pastejo intermitente, recentemente vem sendo estudado em espécies forrageiras em distintas condições climáticas do Brasil, porém, pesquisas avaliando a formação e desenvolvimento de folhas e perfilhos por meio das variáveis morfogênicas e estruturais em função de IAFr são ainda inexistentes em pastos de Tifton 85. Por isso, objetivou-se com este trabalho, avaliar os efeitos de IAFr dos pastos, em ciclos de pastejos sucessivos, sobre as características morfogênicas e estruturais de Tifton 85 pastejados de maneira intermitente por ovinos. Características morfogênicas e estruturais dos pastos foram avaliadas em três ciclos de pastejo em função de três IAFr (0,8; 1,6 e 2,4). O intervalo de rebrotação dos pastos aumentou de 21,33 para 29,66 dias com a diminuição do IAFr. A taxa de aparecimento de folhas aumentou 0,02 folhas perfilho-1dia-1 e o número de folhas vivas por perfilho aumentou 0,41 com a diminuição do IAFr. Filocrono, taxa de alongamento de folhas, comprimento final de folha, taxa de senescência foliar e taxa de alongamento do colmo decresceram linearmente com a diminuição do IAFr dos pastos. Somente o número de folhas vivas por perfilho foi menor (7,97) no terceiro ciclo de pastejo, provavelme

The effect of grazing intensity and supplementation on performance, stress indicators and metabolic profiles of finishing lambs / Efeito de intensidade de pastejo e suplementação no desempenho, indicadores de estresse e perfil metabólico de cordeiros em terminação

In the present study, the effect of grazing intensity (GI) and feed supplementation in sixty-four crossbred Santa Inês lambs was assessed based on performance, blood stress indicators, and metabolic profiles. The GIs analyzed were VH = very high, H = high, L = low, and VL = very low, and were represented by the residual leaf area index (LAIr). The lambs were divided into two groups, supplemented (SP) and non-supplemented (NSP). The dry matter intake (DMI) and average daily gain (ADG) increased linearly with decreasing GI (P 0.05), and the ADG was higher for the NSP group than the SP group (P 0.05). The plasma cortisol concentration was higher in the NSP group (P 0.05), and it was not affected by GIs (P > 0.05). The neutrophil:lymphocyte (N:L) ratio decreased linearly with GI (P 0.05), and the SP group had a higher N:L ratio (P 0.05). None of the parameters evaluated for protein metabolism were affected by GI (P > 0.05), except albumin, where the SP group had a higher concentration of this metabolite (P 0.05). Regarding energy metabolism indicators, glucose showed a linear increase with a decrease in GI (P 0.05) and was higher in the SP group (P 0.05); however, there was a decreasing linear effect (P 0.05) and the NSP group had higher serum levels (P 0.05) of non-esterified fatty acids (NEFA). The concentration of beta...(AU)

No presente estudo foi avaliado o efeito de intensidades de pastejo (IP) pelo índice de área foliar residual (IAFr). Onde, 0,8; 1,4; 2,0 e 2,6 de IAFr corresponde a MA= muito alto, A= alto, B= baixo e MB= muito baixo com suplemento (SP) ou não suplementado (NSP) no desempenho, indicadores de estresse e perfil metabólico em sessenta e quatro cordeiros mestiços Santa Inês (15,40±2,31 kg peso vivo). O consumo de matéria seca (CMS) e ganho médio diário (GMD), em resposta a IP, apresentaram efeito linear crescente (P 0,05) com a diminuição da IP e o grupo NSP foi superior (P 0,05) ao grupo SP quanto ao CMS. A concentração plasmática de cortisol foi maior no grupo NSP (P 0,05) e não apresentou efeito (P>0,05) entre as IP. A relação Neutrófilo:Linfócito apresentou efeito linear decrescente (P 0,05) com a IP e o grupo SPT apresentou maior (P 0,05) relação Neutrófilo:Linfócito. Nenhum dos parâmetros avaliados referentes ao metabolismo proteico foram afetados (P>0,05) pela IP, exceto a Albumina que apresentou efeito (P 0,05) e o grupo SP apresentou maior concentração desse metabólito. Referente aos indicadores do metabolismo energético, a glicose apresentou efeito linear crescente (P 0,05) com o a diminuição da IP e foi maior no grupo SP (P 0,05), de maneira oposta, houve efeito linear decrescente dos ácidos graxos não esterificados (AGNE) (P 0,05)...(AU)

Relative chlorophyll contents in the evaluation of the nutritional status of nitrogen from xaraes palisade grass and determination of critical nitrogen sufficiency index / Teor relativo de clorofila na avaliação do status nutricional de nitrogênio do capimxaraés e determinação do índice de suficiência do nitrogênio crítico

The nutritional status of xaraes palisade grass with SPAD chlorophyll meter and the critical nitrogen sufficiency index (cNSI) in the dry and rainy seasons were assessed. The experiment was conducted in a completely randomized design with four treatments (50, 100, 200 and 400 kg N ha-1), three replicates and two seasons: dry and rainy. All variables had a high nonlinear relationship with their predictors (p 0.001). Total nitrogen (TN) reached a plateau at doses 262.0 and 514.8 kg of N ha-1 and the concentration of TN in the plant was 1.88 and 1.93% respectively during the rainy and dry season. Relative chlorophyll content (RCC) reached a plateau at 46.05 and 53.65 SPAD units in the rainy and dry seasons, respectively. The production of dry matter (DM) showed maximum response to nitrogen fertilization at 209.5 and 229.1 kg N ha-1 during the rainy and dry season respectively. The nitrogen sufficiency index (NSI) reached the plateau at 0.85 and 0.99 in the rainy and dry season respectively. All variables showed high linear correlation (r = 0.71 to 0.99). The xaraés palisade grasss cNSI is 0.85 and the chlorophyll meter may be used as a nutritional N management tool for the grass.(AU)

Objetivou-se avaliar o estado nutricional do capim-xaraés utilizando o clorofilômetro SPAD e estabelecer o índice de suficiência crítico (ISNc) nas épocas das águas e seca. O experimento foi conduzido em um delineamento inteiramente casualizado com quatro tratamentos (50, 100, 200 e 400 kg N ha-1), três repetições, duas épocas: águas e seca. Todas as variáveis apresentaram alta relação não linear com seus preditores (p 0,001). O nitrogênio total (NT) alcançou o platô na dose de 262,0 e 514,8 kg de N ha-1 e a concentração de NT na planta foi 1,88 e 1,93% no período das águas e seco, respectivamente. O teor relativo de clorofila (TRC) atingiu o platô a 46,05 e 53,65 unidades SPAD no período das águas e seca, respectivamente. A produção de matéria seca (MS) apresentou máxima resposta a adubação nitrogenada na dose de 209,5 e 229,1 kg N ha-1 na época das águas e seca, respectivamente. O índice de suficiência de nitrogênio (ISN) atingiu o platô em 0,85 e 0,99 na época das águas e secas respectivamente. Todas as variáveis apresentaram alta correlação linear (r= 0,71 a 0,99). O ISNc do capim-xaraés é 0,85 e o clorofilômetro pode ser utilizado como ferramenta de manejo nutricional de N para essa gramínea.(AU)

Relative chlorophyll contents in the evaluation of the nutritional status of nitrogen from xaraes palisade grass and determination of critical nitrogen sufficiency index / Teor relativo de clorofila na avaliação do status nutricional de nitrogênio do capimxaraés e determinação do índice de suficiência do nitrogênio crítico

The nutritional status of xaraes palisade grass with SPAD chlorophyll meter and the critical nitrogen sufficiency index (cNSI) in the dry and rainy seasons were assessed. The experiment was conducted in a completely randomized design with four treatments (50, 100, 200 and 400 kg N ha-1), three replicates and two seasons: dry and rainy. All variables had a high nonlinear relationship with their predictors (p 0.001). Total nitrogen (TN) reached a plateau at doses 262.0 and 514.8 kg of N ha-1 and the concentration of TN in the plant was 1.88 and 1.93% respectively during the rainy and dry season. Relative chlorophyll content (RCC) reached a plateau at 46.05 and 53.65 SPAD units in the rainy and dry seasons, respectively. The production of dry matter (DM) showed maximum response to nitrogen fertilization at 209.5 and 229.1 kg N ha-1 during the rainy and dry season respectively. The nitrogen sufficiency index (NSI) reached the plateau at 0.85 and 0.99 in the rainy and dry season respectively. All variables showed high linear correlation (r = 0.71 to 0.99). The xaraés palisade grasss cNSI is 0.85 and the chlorophyll meter may be used as a nutritional N management tool for the grass.

Objetivou-se avaliar o estado nutricional do capim-xaraés utilizando o clorofilômetro SPAD e estabelecer o índice de suficiência crítico (ISNc) nas épocas das águas e seca. O experimento foi conduzido em um delineamento inteiramente casualizado com quatro tratamentos (50, 100, 200 e 400 kg N ha-1), três repetições, duas épocas: águas e seca. Todas as variáveis apresentaram alta relação não linear com seus preditores (p 0,001). O nitrogênio total (NT) alcançou o platô na dose de 262,0 e 514,8 kg de N ha-1 e a concentração de NT na planta foi 1,88 e 1,93% no período das águas e seco, respectivamente. O teor relativo de clorofila (TRC) atingiu o platô a 46,05 e 53,65 unidades SPAD no período das águas e seca, respectivamente. A produção de matéria seca (MS) apresentou máxima resposta a adubação nitrogenada na dose de 209,5 e 229,1 kg N ha-1 na época das águas e seca, respectivamente. O índice de suficiência de nitrogênio (ISN) atingiu o platô em 0,85 e 0,99 na época das águas e secas respectivamente. Todas as variáveis apresentaram alta correlação linear (r= 0,71 a 0,99). O ISNc do capim-xaraés é 0,85 e o clorofilômetro pode ser utilizado como ferramenta de manejo nutricional de N para essa gramínea.

The effect of grazing intensity and supplementation on performance, stress indicators and metabolic profiles of finishing lambs / Efeito de intensidade de pastejo e suplementação no desempenho, indicadores de estresse e perfil metabólico de cordeiros em terminação

In the present study, the effect of grazing intensity (GI) and feed supplementation in sixty-four crossbred Santa Inês lambs was assessed based on performance, blood stress indicators, and metabolic profiles. The GIs analyzed were VH = very high, H = high, L = low, and VL = very low, and were represented by the residual leaf area index (LAIr). The lambs were divided into two groups, supplemented (SP) and non-supplemented (NSP). The dry matter intake (DMI) and average daily gain (ADG) increased linearly with decreasing GI (P 0.05), and the ADG was higher for the NSP group than the SP group (P 0.05). The plasma cortisol concentration was higher in the NSP group (P 0.05), and it was not affected by GIs (P > 0.05). The neutrophil:lymphocyte (N:L) ratio decreased linearly with GI (P 0.05), and the SP group had a higher N:L ratio (P 0.05). None of the parameters evaluated for protein metabolism were affected by GI (P > 0.05), except albumin, where the SP group had a higher concentration of this metabolite (P 0.05). Regarding energy metabolism indicators, glucose showed a linear increase with a decrease in GI (P 0.05) and was higher in the SP group (P 0.05); however, there was a decreasing linear effect (P 0.05) and the NSP group had higher serum levels (P 0.05) of non-esterified fatty acids (NEFA). The concentration of beta...

No presente estudo foi avaliado o efeito de intensidades de pastejo (IP) pelo índice de área foliar residual (IAFr). Onde, 0,8; 1,4; 2,0 e 2,6 de IAFr corresponde a MA= muito alto, A= alto, B= baixo e MB= muito baixo com suplemento (SP) ou não suplementado (NSP) no desempenho, indicadores de estresse e perfil metabólico em sessenta e quatro cordeiros mestiços Santa Inês (15,40±2,31 kg peso vivo). O consumo de matéria seca (CMS) e ganho médio diário (GMD), em resposta a IP, apresentaram efeito linear crescente (P 0,05) com a diminuição da IP e o grupo NSP foi superior (P 0,05) ao grupo SP quanto ao CMS. A concentração plasmática de cortisol foi maior no grupo NSP (P 0,05) e não apresentou efeito (P>0,05) entre as IP. A relação Neutrófilo:Linfócito apresentou efeito linear decrescente (P 0,05) com a IP e o grupo SPT apresentou maior (P 0,05) relação Neutrófilo:Linfócito. Nenhum dos parâmetros avaliados referentes ao metabolismo proteico foram afetados (P>0,05) pela IP, exceto a Albumina que apresentou efeito (P 0,05) e o grupo SP apresentou maior concentração desse metabólito. Referente aos indicadores do metabolismo energético, a glicose apresentou efeito linear crescente (P 0,05) com o a diminuição da IP e foi maior no grupo SP (P 0,05), de maneira oposta, houve efeito linear decrescente dos ácidos graxos não esterificados (AGNE) (P 0,05)...

Tanzania grass structure grazed by goats managed with different residual leaf area index under intermittent stocking / Estrutura do capim Tanzânia manejado em diferentes índices de área foliar residual pastejado por caprinos sob lotação intermitente

The objective of this study was to evaluate the structure of Tanzania grassland grazed by goats managed with different residue leaf area index (RLAI) under intermittent stocking. The experiment was carried out from February to August, 2008. The treatments consisted of three different targets RLAI (0.8, 1.6 and 2.4) and 95% light interception (LI) criterion determined the rest period. Forage samples were collected at average height sampling points and weighed. Subsequently, a smaller sample was removed to separate the morphological components (leaf, stem and dead material) and to determine the structural and productive features. The canopy architecture was evaluated by the method of inclined point quadrat. The pre-grazing height in the paddocks were significantly different among treatments. RLAI influenced dry matter contents of green forage, leaf, stem and total, with the exception of dry matter of dead material, where the lowest values were observed for 0.8 RLAI. Thus, RLAI modifies canopy structure and is sensitive to canopy height changes throughout the year. Pasture regrowth is not compromised by residual leaf area indexes between 0.8 and 2.4, when climatic factors are not limiting.

O objetivo deste estudo foi avaliar a estrutura do capim Tanzânia manejado com diferentes índices de área foliar residual (IAFR) pastejado por caprinos sob lotação intermitente. O experimento ocorreu de fevereiro a agosto de 2008. Os tratamentos consistiram em três diferentes estratégias de IAFR (0,8, 1,6 e 2,4) e o critério de 95% de interceptação luminosa (IL) para determinação do período de descanso. Amostras de forragem em pontos de altura média foram colhidas e pesadas. Posteriormente, uma subamostra foi colhida para separação dos componentes morfológicos (folha, colmo e material morto) e para determinação das características estruturais e produtivas. A arquitetura do dossel foi avaliada pelo método do ponto inclinado. No pré-pastejo, os valores de altura dos piquetes foram diferentes entre os tratamentos. O IAFR influenciou a massa seca dos componentes de forragem verde, folha, colmo e total, com exceção da massa seca de material morto, onde foram observados menores valores no resíduo de 0,8 IAFR. Portanto, o IAFR modifica a estrutura da planta e é sensível a mudanças na altura do dossel ao longo do ano. Índices de área foliar residual entre 0,8 e 2,4 não comprometem a rebrota do pasto, quando os fatores climáticos não são limitantes.

Positioning and number of nutritional levels in dose-response trials to estimate the optimal-level and the adjustment of the models / Posição e número de níveis nutricionais em experimentos dose-resposta na estimativa do nível-ótimo e ajuste dos modelos

The aim of this research was to evaluate the influence of the number and position of nutrient levels used in dose-response trials in the estimation of the optimal-level (OL) and the goodness of fit on the models: quadratic polynomial (QP), exponential (EXP), linear response plateau (LRP) and quadratic response plateau (QRP). It was used data from dose-response trials realized in FCAV-Unesp Jaboticabal considering the homogeneity of variances and normal distribution. The fit of the models were evaluated considered the following statistics: adjusted coefficient of determination (R²adj), coefficient of variation (CV) and the sum of the squares of deviations (SSD).It was verified in QP and EXP models that small changes on the placement and distribution of the levels caused great changes in the estimation of the OL. The LRP model was deeply influenced by the absence or presence of the level between the response and stabilization phases (change in the straight to plateau). The QRP needed more levels on the response phase and the last level on stabilization phase to estimate correctly the plateau. It was concluded that the OL and the adjust of the models are dependent on the positioning and the number of the levels and the specific characteristics of each model, but levels defined near to the true requirement and not so spaced are better to estimate the OL.(AU)

O objetivo deste trabalho foi avaliar a influência do número e posição de níveis nutricionais utilizados em ensaios dose-resposta na estimativa do nível-ótimo (OL) e ajuste dos modelos polinomial quadrático (QP), exponencial (EXP), linear response plateau (LRP) e quadratic respose plateau (QRP). Utilizaram-se dados provenientes de ensaios dose-resposta realizados na FCAV-Unesp Jaboticabal, atendendo as pressuposições de homocedasticidade e normalidade. O ajuste dos modelos foi avaliado considerando as seguintes estatísticas: coeficiente de determinação ajustado (R²adj), coeficiente de variação (CV) e soma dos quadrados dos desvios (SSD).Verificou-se que, nos modelos QP e EXP, pequenas mudanças na localização e distribuição dos níveis ocasionam grandes alterações na estimativa do OL. O modelo LRP foi influenciado pela ausência ou presença do nível intermediário às fases de resposta e estabilização (mudança da reta crescente para platô). O modelo QRP precisou de um número maior de níveis na fase de resposta e o último nível da fase de estabilização para estimar corretamente o platô. Pôde-se concluir que a determinação do OL e o ajuste dos modelos dependem da posição e quantidade de níveis, além das características específicas de cada modelo, mas níveis definidos próximos do verdadeiro requerimento e não muito espaçados são melhores para estimar corretamente o OL.(AU)

Positioning and number of nutritional levels in dose-response trials to estimate the optimal-level and the adjustment of the models / Posição e número de níveis nutricionais em experimentos dose-resposta na estimativa do nível-ótimo e ajuste dos modelos

The aim of this research was to evaluate the influence of the number and position of nutrient levels used in dose-response trials in the estimation of the optimal-level (OL) and the goodness of fit on the models: quadratic polynomial (QP), exponential (EXP), linear response plateau (LRP) and quadratic response plateau (QRP). It was used data from dose-response trials realized in FCAV-Unesp Jaboticabal considering the homogeneity of variances and normal distribution. The fit of the models were evaluated considered the following statistics: adjusted coefficient of determination (R²adj), coefficient of variation (CV) and the sum of the squares of deviations (SSD).It was verified in QP and EXP models that small changes on the placement and distribution of the levels caused great changes in the estimation of the OL. The LRP model was deeply influenced by the absence or presence of the level between the response and stabilization phases (change in the straight to plateau). The QRP needed more levels on the response phase and the last level on stabilization phase to estimate correctly the plateau. It was concluded that the OL and the adjust of the models are dependent on the positioning and the number of the levels and the specific characteristics of each model, but levels defined near to the true requirement and not so spaced are better to estimate the OL.

O objetivo deste trabalho foi avaliar a influência do número e posição de níveis nutricionais utilizados em ensaios dose-resposta na estimativa do nível-ótimo (OL) e ajuste dos modelos polinomial quadrático (QP), exponencial (EXP), linear response plateau (LRP) e quadratic respose plateau (QRP). Utilizaram-se dados provenientes de ensaios dose-resposta realizados na FCAV-Unesp Jaboticabal, atendendo as pressuposições de homocedasticidade e normalidade. O ajuste dos modelos foi avaliado considerando as seguintes estatísticas: coeficiente de determinação ajustado (R²adj), coeficiente de variação (CV) e soma dos quadrados dos desvios (SSD).Verificou-se que, nos modelos QP e EXP, pequenas mudanças na localização e distribuição dos níveis ocasionam grandes alterações na estimativa do OL. O modelo LRP foi influenciado pela ausência ou presença do nível intermediário às fases de resposta e estabilização (mudança da reta crescente para platô). O modelo QRP precisou de um número maior de níveis na fase de resposta e o último nível da fase de estabilização para estimar corretamente o platô. Pôde-se concluir que a determinação do OL e o ajuste dos modelos dependem da posição e quantidade de níveis, além das características específicas de cada modelo, mas níveis definidos próximos do verdadeiro requerimento e não muito espaçados são melhores para estimar corretamente o OL.