First report of Cryptosporidium sp. infection in sheep population of Ladakh, India.

Ladakh is the high altitude (11,184 ft above sea level) cold desert region in the state of Jammu & Kashmir (India), where winter temperature goes below - 35 °C. A total of 37 faecal samples were collected from local Changthangi and Russian merino sheep of the Leh region of Ladakh, having the history of diarrhoea and emaciation, followed by death. Of the 37 samples collected, 20 (54.05%) showed presence of Cryptosporidium sp. infection on faecal smear examination after Ziehl-Neelsen staining. The number of samples positive for Cryptosporidium sp. infection on faecal smear examination was 7 and 13 for Russian Merino and local Changthangi sheep, respectively. The occurrence of Cryptosporidium sp. infection was similar in both sexes, 11/20 (55%) in females and 9/20 (45%) for males. The Age-wise occurrence of Cryptosporidium sp. infection in different age groups was found to be: 50% in less than 2 year age group and 50% in more than 2 year age group. To the best of our knowledge, this is the first report of Cryptosporidium sp. infection in adult sheep from India. The survival of the parasite at such freezing temperature gives the insight about its cold-resistant nature.

Minimally invasive surgical tube cystotomy for treating obstructive urolithiasis in small ruminants with an intact urinary bladder.

Ten young, male, entire small ruminants (seven kids and three lambs) with obstructive urolithiasis, which were presented within three days of complete blockage and before rupture of the urinary bladder and urethra, underwent a minimally invasive surgical tube cystotomy through the left paralumbar fossa. The catheter was placed in the bladder lumen through a metallic cannula and fixed to the skin with a stay suture. Surgery was performed with the animal standing (six cases) or in right lateral recumbency (four cases) on the day of presentation. All animals were discharged the same day. Eight animals urinated normally within a mean of seven days (range four to 10 days). One animal had a blockage of urine flow as a result of kinking of the catheter on the third postoperative day, and in another the catheter collapsed on the fourth postoperative day. These were managed by a second, conventional surgical tube cystotomy. No recurrence of the condition was noticed in any of the animals during a six-month follow-up period.