Gut dysbiosis impacts the immune system and promotes prostate cancer.

The gut microbiota is a system of microorganisms in the human gastrointestinal (GI) system, consisting of trillions of microorganisms residing in epithelial surfaces of the body. Gut microbiota are exposed to various external and internal factors and form a unique gut-associated immunity maintained through a balancing act among diverse groups of microorganisms. The role of microbiota in dysbiosis of the gut in aiding prostate cancer development has created an urgency for extending research toward comprehension and preventative measures. The gut microbiota varies among persons based on diet, race, genetic background, and geographic location. Bacteriome, mainly, has been linked to GI complications, metabolism, weight gain, and high blood sugar. Studies have shown that manipulating the microbiome (bacteriome, virome, and mycobiome) through the dietary intake of phytochemicals positively influences physical and emotional health, preventing and delaying diseases caused by microbiota. In this review, we discuss the wealth of knowledge about the GI tract and factors associated with dysbiosis-mediated compromised gut immunity. This review also focuses on the relationship of dysbiosis to prostate cancer, the impact of microbial metabolites short-chain fatty acids (SCFAs) on host health, and the phytochemicals improving health while inhibiting prostate cancer.

The presence/absence of conspecifics modulates the circadian locomotor activity and body mass in spotted munia (Lonchura punctulata).

Biological clocks regulate the behavior and physiology of animals by tracking the local time using diverse time cues. Social cues are relevant in studying the behavior of gregarious animals, but these cues have not been widely studied in birds. Temporal information for circadian timekeeping is socially communicated through visual, physical, olfactory, and auditory means. We examined the efficacy of pulsatile social interactions on locomotor activity and its associated characteristics such as distribution profile of rest and activity, total counts, activity duration, phase shift in activity onset, and circadian periodicity in spotted munia. Besides, we analyzed the effect of such social interactions on their body mass. Spotted munia exhibited phase shift in the onset of activity when subjected to social isolation, but these cues could not affect their circadian periodicity. In Pair as well as in Group, social isolation led to increased activity and activity duration, and decreased body mass in guests relative to the host bird. Our results suggest that the circadian rhythm of locomotor activity in spotted munia is quite sensitive to socialization and isolation, and isolation is detrimental for the birds. Consistent with these observations, the decline in body mass revealed the physiological consequences of social isolation on spotted munia.

Automated qualitative batch measurement of lipid droplets in the liver of bird using ImageJ.

Oil red O staining is effective in detection and quantification of neutral lipid droplets in tissues such as the liver. However, converting images into testable data using ImageJ can be time-consuming and is prone to inaccuracy or bias. We describe a protocol for automated qualitative measurement of lipid droplets in the bird liver using a batch processing macro script. We explain steps for extracting tissue, cryosectioning, staining, and imaging, followed by script generation for quantification of lipid in the images.

Surviving high temperatures: a case study of the spotted munia (Lonchura punctulata).

Every year, a combination of summer with extreme weather events such as "heatwaves" affects the life of organisms on earth. Previous studies on humans, rodents, and some birds signify the impact of heat stress on their survival and existence. Over the past four decades, the frequency of heatwaves has increased because of global warming. Therefore, we performed a longitudinal study on a resident bird species, the spotted munia (Lonchura punctulata) by simulating a heatwave-like condition. We were interested in understanding how a Passeriformes native to a sub-tropical country deals with heatwave-like conditions. Initially, the birds were subjected to room temperature (25 ± 2 °C; T1) for 10 days, followed by a simulated heatwave-like condition (42 ± 1 °C; T2) for 7 days and again back to room temperature (25 ± 2 °C; RT1) for the next 7 days. To elucidate how birds cope with simulated heatwave conditions, we examined different behavioral and physiological parameters. We found that although heat stress significantly reduced total activity counts and food intake but, the body mass, blood glucose, and hemoglobin levels remained unaffected by any of the temperature conditions. Furthermore, HSP70 and biochemical markers of liver injuries such as ALP, AST, ALT, bilirubin direct, and bilirubin total were found elevated in response to the simulated heatwave-like condition, whereas uric acid and triglyceride were reduced. Creatinine and total protein levels were unaffected by the heatwave. The post heatwave treatment resulted in a rebound of the behavioral and physiological responses, but the recovered responses were not equivalent to the pre-heatwave levels (T1 conditions). Thus, the present study demonstrates heatwave-associated behavioral and physiological changes in a resident passerine finch which has tremendous physiological flexibility.

Photoperiod-driven concurrent changes in hypothalamic and brainstem transcription of sleep and immune genes in migratory redheaded bunting.

The molecular regulation of sleep in avian migrants is still obscure. We thus investigated this in migratory redheaded buntings, where four life-history states (LHS; i.e. non-migratory, pre-migratory, migratory and refractory states) were induced. There was increased night-time activity (i.e. Zugunruhe) during the migratory state with reduced daytime activity. The recordings of the sleep-wake cycle in buntings showed increased night-time active wakefulness coupled with drastically reduced front and back sleep during migratory phase. Interestingly, we found the buntings to feed and drink even after lights-off during migration. Gene expression studies revealed increased hypothalamic expression of glucocorticoid receptor (nr3c1), and pro-inflammatory cytokines (il1b and il6) in pre-migratory and migratory states, respectively, whereas in brainstem Ca2+/calmodulin-dependent protein kinase 2 (camk2) was upregulated during the migratory state. This suggested a heightened pro-inflammatory state during migration which is a feature of chronic sleep loss, and a possible role of Ca2+ signalling in promoting wakefulness. In both the hypothalamus and brainstem, the expression of melatonin receptors (mel1a and mel1b) was increased in the pre-migratory state, and growth hormone-releasing hormone (ghrh, known to induce sleep) was reduced during the migratory state. The current results demonstrate key molecules involved in the regulation of sleep-wake cycle across LHS in migratory songbirds.

Photoperiodic modulation of melatonin receptor and immune genes in migratory redheaded bunting.

The transcriptional regulation of innate immune function across annual life history states (LHS) remains obscure in avian migrants. We, therefore, investigated this in a migratory passerine songbird, redheaded bunting (Emberiza bruniceps), which exhibits long-distance vernal migration from India to Central Asia. We exposed the birds (N = 10) to differential photoperiodic conditions to induce a non-migratory (NM), pre-migratory (PM), migratory (MIG), and refractory (REF) state, and performed gene expression assays of melatonin receptors (MEL1A and MEL1B), and innate immunity-linked genes (IL1B, IL6, TLR4, and NFKB) in spleen and blood. We found a significant reduction in splenic mass and volume, and a parallel increase in fat accumulation, and testicular growth in birds under migratory state. The gene expression assay revealed an upregulation of MEL1A and MEL1B mRNA levels in both the tissues in MIG. Additionally, we found a nocturnal increase of splenic IL1B expression, and IL1B, IL6, and TLR4 expression in the blood. The mRNA expression of melatonin receptors and proinflammatory cytokine showed a positive correlation. These results suggest that melatonin relays the photoperiodic signal to peripheral immune organs, which shows LHS-dependent changes in mRNA expression of immune genes.

Evaluation of social jetlag and chronotypes in Indian school-going adolescents.

Background: School-going students' school time acts as a zeitgeber during the weekdays, and in the weekends, due to the absence of the zeitgeber, they show free run and wake up late in the morning. This may result in poor health hazards in adolescents. Objective: The aim of this study was to assess the prevalence of social jetlag along with the distribution of their chronotype in school-going adolescents. Materials and Methods: This is a cross-sectional study conducted between May and June 2019 with the help of the MCTQ (Munich Chronotype Questionnaire) and MEQ (Morningness and Eveningness Questionnaire) in the adolescent population (N = 1154). Statistical Analysis: The study was performed using the Mann-Whitney U test to compare continuous variables of sleep parameters between workdays and free days. The significance was taken at P < 0.05. Results: The results showed that out of the total population, 81% were morning type and 67% of adolescents were socially jetlagged. There was a significant delay in the time to bed on free days by 1.88 hours and a significant delay in the wake-up time by 2.46 hours. The total sleep duration was also significantly higher on free days by 1.88 hours in the population. Conclusion: This is our first study in the Indian population to show that morning-type individuals showed irregular sleep timings and were more socially jetlagged. This needs to be focused on by the school authorities and government to keep a flexible school timing for the students on the basis of their chronotype for their better academic performance.

The unlock consequences: changes in daily behaviors and mental health in Indian population during the second wave of COVID-19.

Objectives: The COVID-19 outbreak has led to unprecedented changes throughout the world. It has imposed lockdown, social distancing to avoid the spread of this disease. India in the middle of March 2021 reported the beginning of the second wave of corona leading to massive death. We hypothesized to investigate the effect of sleep and eating behavior also got affected during unlock imposed due to the second wave. Material and Methods: The data collection was done by using an online google form by making them available to participants through various social media apps via smartphone. Total participants (n=115) mean age was 25.86±9.52 (Mean±SD). The results were analyzed by using RM One-Way ANOVA, Pearson correlation test by using SPSS 26. Results: We found that the sleep behavior including time to sleep which was delayed by 46min, time to wake up was 58min, and sleep duration was increased by 6min during the unlock days when compared with weekdays before unlock. The eating behavior during unlock including the time of breakfast, lunch, and dinner was delayed by 1-hour 3min, 23min, and 19min, respectively. The social jetlag was reduced by 6min and eating jetlag was increased by approximately 8.4min. We found a strong positive correlation between eating jetlag and social jetlag during unlock (r=0.262, p<0.005). Conclusion: Our findings can help in modifying irregular sleep and unhealthy eating behaviors into a good and healthy lifestyle which will, in turn, lead to a depression-free lifestyle during unlock/lockdown due to the COVID-19 pandemic.

Effect of artificial light at night on sleep and metabolism in weaver birds.

Artificial light at night is constantly minimizing the span of dark nights from the natural light-dark cycle of earth. Over the past century, the "lightscape" of earth has completely changed owing to technological advancements which subsequently changed the lifestyle of human as well as the nearby animal species. This motivated the present study, wherein we investigated the impact of light at night (LAN) on behavior and physiology of a diurnal passerine finch, baya weaver (Ploceus philippinus). A group of bird (N=10) exposed to 12L:12D photoperiod was initially subjected to dark nights (0 lux) for a period of 1.5 weeks followed by 5 lux, night light for a span of 4 weeks. The first week in LAN served as acute treatment with respect to the fourth week (chronic). The results reveal significant increase in nighttime activity and sleep loss with respect to acute LAN, while significant inclusion of drowsiness behavior during the day in response to chronic LAN. Besides these behavioral alterations, changes in physiological parameters such as reduction in body mass, loss of gradient between pre- and post- prandial blood glucose levels, and elevation in plasma corticosterone levels were more prominent during acute exposure of LAN. Plasma metabolites such as triglycerides, total protein, serum glutamic-oxaloacetic transaminase (SGOT), and creatinine concentrations also hiked in response to acute LAN treatment. Thus, acute exposure of LAN seems to serve as a novel environment for the bird leading to more pronounced impacts on behavioral and physiological observations during the experiment. In chronic exposure, the birds sort of adapted themselves to the prevailing circumstances as evident by decreased nighttime activity, rebound of sleep and corticosterone levels, etc. Thus, the study clearly demonstrates the differential impact of acute and chronic exposure of LAN on behavior and physiology of birds.

Understanding the significance of biological clock and its impact on cancer incidence.

The circadian clock is an essential timekeeper that controls, for humans, the daily rhythm of biochemical, physiological, and behavioral functions. Irregular performance or disruption in circadian rhythms results in various diseases, including cancer. As a factor in cancer development, perturbations in circadian rhythms can affect circadian homeostasis in energy balance, lead to alterations in the cell cycle, and cause dysregulation of chromatin remodeling. However, knowledge gaps remain in our understanding of the relationship between the circadian clock and cancer. Therefore, a mechanistic understanding by which circadian disruption enhances cancer risk is needed. This review article outlines the importance of the circadian clock in tumorigenesis and summarizes underlying mechanisms in the clock and its carcinogenic mechanisms, highlighting advances in chronotherapy for cancer treatment.

Photoperiodically driven transcriptome-wide changes in the hypothalamus reveal transcriptional differences between physiologically contrasting seasonal life-history states in migratory songbirds.

We investigated time course of photoperiodically driven transcriptional responses in physiologically contrasting seasonal life-history states in migratory blackheaded buntings. Birds exhibiting unstimulated winter phenotype (photosensitive state; responsive to photostimulation) under 6-h short days, and regressed summer phenotype (photorefractory state; unresponsiveness to photostimulation) under 16-h long days, were released into an extended light period up to 22 h of the day. Increased tshß and dio2, and decreased dio3 mRNA levels in hypothalamus, and low prdx4 and high il1ß mRNA levels in blood confirmed photoperiodic induction by hour 18 in photosensitive birds. Further, at hours 10, 14, 18 and 22 of light exposure, the comparison of hypothalamus RNA-Seq results revealed transcriptional differences within and between states. Particularly, we found reduced expression at hour 14 of transthyretin and proopiomelanocortin receptor, and increased expression at hour 18 of apolipoprotein A1 and carbon metabolism related genes in the photosensitive state. Similarly, valine, leucine and isoleucine degradation pathway genes and superoxide dismutase 1 were upregulated, and cocaine- and amphetamine-regulated transcript and gastrin-releasing peptide were downregulated in the photosensitive state. These results show life-history-dependent activation of hypothalamic molecular pathways involved in initiation and maintenance of key biological processes as early as on the first long day.

Optimum health and inhibition of cancer progression by microbiome and resveratrol.

Resveratrol (RES) is a naturally occurring polyphenol found in fruits, green leafy vegetables, and peanuts. This versatile compound, which has potent regenerative, anti-oxidative, and cancer-fighting properties, is produced in plants, particularly in response to stress stimuli. By various mechanisms, including regulation of genes and proteins, RES inhibits the growth of pathogenic bacteria and the development of cancers. The gut has a prominent role in nutrient assimilation, metabolism, immunity, and cancer regression, and the endogenous microbiome protects the host from invasive bacteria that facilitate the progression of various diseases. Short-chain fatty acids (SFCAs) are the byproducts of microbial fermentation in the gastrointestinal tract. Native microflora regulates internal homeostasis, influence the activity of host immune cells, and regress some cancers via the action of SCFAs produced from a plant-based diet. This review shows the relevance of dietary constituents and gut microbial activity in ensuring optimal health of the host.

Hypothalamic plasticity in response to changes in photoperiod and food quality: An adaptation to support pre-migratory fattening in songbirds?

In latitudinal avian migrants, increasing photoperiods induce fat deposition and body mass increase, and subsequent night-time migratory restlessness in captive birds, but the underlying mechanisms remain poorly understood. We hypothesized that an enhanced hypothalamic neuronal plasticity was associated with the photostimulated spring migration phenotype. We tested this idea in adult migratory red-headed buntings (Emberiza bruniceps), as compared with resident Indian weaverbirds (Ploceus philippinus). Birds were exposed to a stimulatory long photoperiod (14L:10D, LP), while controls were kept on a short photoperiod (10L:14D, SP). Under both photoperiods, one half of birds also received a high calorie, protein- and fat-rich diet (SP-R, LP-R) while the other half stayed on the normal diet (SP-N, LP-N). Thirty days later, as expected, the LP had induced multiple changes in the behaviour and physiology in migratory buntings. Photostimulated buntings also developed a preference for the rich food diet. Most interestingly, the LP and the rich diet, both separately and in association, increased neurogenesis in the mediobasal hypothalamus (MBH), as measured by an increased number of cells immunoreactive for doublecortin (DCX), a marker of recently born neurons, in buntings, but not weaverbirds. This neurogenesis was associated with an increased density of fibres immunoreactive for the orexigenic neuropeptide Y (NPY). This hypothalamic plasticity observed in a migratory, but not in a non-migratory, species in response to photoperiod and food quality might represent an adaptation to the pre-migratory fattening, as required to support the extensive energy expenses that incur during the migratory flight.

Ambient temperature affects multiple drivers of physiology and behaviour: adaptation for timely departure of obligate spring migrants.

We investigated the role of ambient temperature in departure from wintering areas of migratory black-headed buntings in spring. Birds transferred at 22 and 35°C to long days were compared with one another and with controls held on short days for indices of readiness to migrate (Zugunruhe, fattening, mass gain), levels of testosterone and gonadal recrudescence. Temperature affected the development of migratory behaviour and physiology: buntings under long days at 35°C, compared with those at 22°C, showed altered migratory behaviour (daily activity and Zugunruhe onset), and enhanced muscle growth and plasma testosterone levels, but showed no effect on testis growth. Temperature was perceived at both peripheral and central levels, and affected multiple molecular drivers culminating into the migratory phenotype. This was evidenced by post-mortem comparison of the expression of 13 genes with known functions in the skin (temperature-sensitive TRP channels: trpv4 and trpm8), hypothalamus and/or midbrain (migration-linked genes: th, ddc, adcyap1 and vps13a) and flight muscles (muscle growth associated genes: ar, srd5a3, pvalb, mtor, myod, mstn and hif1a). In photostimulated birds, the expression of trpv4 in skin, th in the hypothalamus and midbrain, and srd5a3, ar, pvalb and mtor in flight muscle, in parallel with testosterone levels, was greater at 35°C than at 22°C. These results demonstrate the role of ambient temperature in development of the spring migration phenotype, and suggest that transcriptional responsiveness to temperature is a component of the overall adaptive strategy in latitudinal songbird migrants for timely departure from wintering areas in spring.

Concurrent changes in photoperiod-induced seasonal phenotypes and hypothalamic CART peptide-containing systems in night-migratory redheaded buntings.

This study tested the hypothesis whether hypothalamic cocaine-and amphetamine-regulated transcript (CART)-containing systems were involved in photoperiod-induced responses associated with spring migration (hyperphagia and weight gain) and reproduction (gonadal maturation) in migratory songbirds. We specifically chose CART to examine neural mechanism(s) underlying photoperiod-induced responses, since it is a potent anorectic neuropeptide and involved in the regulation of changes in the body mass and reproduction in mammals. We first studied the distribution of CART-immunoreactivity in the hypothalamus of migratory redheaded buntings (Emberiza bruniceps). CART-immunoreactive neurons were found extensively distributed in the preoptic, lateral hypothalamic (LHN), anterior hypothalamic (AN), suprachiasmatic (SCN), paraventricular (PVN), dorsomedialis hypothalami (DMN), inferior hypothalamic (IH), and infundibular (IN) nuclei. Then, we correlated hypothalamic CART-immunoreactivity in buntings with photostimulated seasonal states, particularly winter non-migratory/non-breeding (NMB) state under short days, and spring premigratory/pre-breeding (PMB) and migratory/breeding (MB) states under long days. There were significantly increased CART-immunoreactive cells, and percent fluorescent area of CART-immunoreactivity was significantly increased in all mapped hypothalamic areas, except the SCN, PVN, AN, and DMN in photostimulated PMB and MB states, as compared to the non-stimulated NMB state. In particular, CART was richly expressed in the medial preoptic nucleus, LHN, IH and IN during MB state in which buntings showed reduced food intake and increased night-time activity. These results suggest that changes in the activity of the CART-containing system in different brain regions were associated with heightened energy needs of the photoperiod-induced seasonal responses during spring migration and reproduction in migratory songbirds.

Neuromorphometric changes associated with photostimulated migratory phenotype in the Palaearctic-Indian male redheaded bunting.

Neural substrates, including brain areas, differential gene expression and neuroendocrine basis, of migration are known. However, very little is known about structural changes in the brain that underlie the development and cessation of migration in long-distance avian migrants. Towards this, we investigated neuromorphological changes in the higher-order associative areas in male redheaded bunting (Emberiza bruniceps), which is a Palaearctic-Indian night migrant with wintering grounds in India. Photosensitive birds (8L:16D; SD) were exposed to stimulatory long days (16L:8D; LD), with controls retained on non-stimulatory short days. LD birds depicted shifts to, and sustained night-time activity as recorded by actograms. LD birds demonstrated increased body mass, fat deposition and testicular volume in keeping with the migratory phenotype. When LD birds had exhibited 10.0 ± 2.4 cycles of Zugunruhe (intense nighttime activity in captives, akin to night migratory flight in the wild), bird brains were fixed by transcardial perfusion, and changes in the neuronal morphometry of pallial, sub-pallial and hypothalamic brain regions studied using rapid Golgi technique with modifications, as used and validated in our laboratory. There were significant differences in both area and perimeter of soma in the visual hyperpallium apicale implicated in migratory orientation and the neuroendocrine control region for timing of migration, the mediobasal hypothalamus. We attribute these neuromorphometric changes in the soma area and perimeter to the photostimulated changes associated with the development of migration and reproductive phenotypes in redheaded buntings. It is suggested that changes in the neuronal plasticity in brain control regions parallel photoperiod-induced physiological responses.

Seasonal reproductive state determines gene expression in the hypothalamus of a latitudinal migratory songbird during the spring and autumn migration.

We investigated gonadal effects on hypothalamic transcription of genes in sham-operated and castrated redheaded buntings photostimulated into spring and autumn migratory states. RNA-Seq results showed testes-dependent differences between spring and autumn migratory states. In particular, differentially expressed genes enriched G-protein-coupled receptor and calcium-ion signaling pathways during spring and autumn states, respectively. qPCR assay showed attenuated gabra5, ttr, thra and thrb expressions, suggesting reduced GABA and thyroid hormone effects on photo-sexual response in spring. In spring castrates, reduced npy, tac1 and nrcam and increased ank3 expression suggested testicular effects on the appetite, prolactin release and neuronal functions, whereas in autumn castrates, reduced rasgrp1, grm5 and grin1, and increased mras expression suggested testicular effects on the ras, G-protein and glutamate signaling pathways. Castration-induced reciprocal switching of pomc and pdyn expressions suggested effects on the overall homeostasis in both seasons. These results demonstrate transcriptome-wide changes, with season-dependent roles of testes in songbird migration.

Development of vernal migration in redheaded buntings: concurrent behavioral, physiological and neural changes under stimulatory photoperiods.

We investigated changes in behavior, physiology and selected brain regions during the development of vernal migration and reproduction phenotypes in migratory redheaded buntings. We monitored 24 h activity-rest pattern and measured food intake, fat deposition, and body mass of buntings exposed for 12 weeks to short (SP, 8L : 16D) and long (LP, 13L : 11D) photoperiods at 22 ± 2 °C temperature. Under LP, not SP, buntings exhibited a photostimulated spring migration phenotype (hyperphagia, fat deposition and body mass gain). However, there were sex differences in the development of vernal migration, as shown by faster and earlier induction of Zugunruhe (nocturnal migratory restlessness) in males than in females. In the next experiment, increasing photoperiods over 12 weeks following the vernal equinox induced behavioural and physiological changes associated with vernal migration phenotypes in both male and female buntings, but in a sex-dependent manner. In a subsequent experiment over 8 weeks corresponding to the spring migration period we found an increased expression of CART, not NPY, in INc, and decreased expression of GnRH-I in POA in the brain by week 6 of the observation under increasing photoperiods. There was also an increased expression of doublecortin (a marker of neuronal incorporation) in the olfactory bulb and song control nuclei (Area X and HVC, higher vocal centre) in male birds. These results demonstrate changes in the brain peptides and neuronal recruitment along with changes in the behaviour and physiology, and give insights into the concurrent photoperiodic induction of the seasonal response at multiple levels in migratory songbirds.

Difference in control between spring and autumn migration in birds: insight from seasonal changes in hypothalamic gene expression in captive buntings.

We hypothesized differences in molecular strategies for similar journeys that migrants undertake to reproduce in spring and to overwinter in autumn. We tested this in redheaded buntings (Emberiza bruniceps) photoinduced into spring and autumn migratory states, with winter and summer non-migratory states as controls. Compared with controls, buntings fattened, gained weight and showed Zugunruhe (nocturnal migratory restlessness) in the migratory state. Spring migration was associated with greater fat and body mass, and higher intensity of Zugunruhe, compared with autumn migration. Circulating corticosterone levels were higher in spring, while T3 levels were higher in autumn. Hypothalamic expression of thyroid hormone-responsive (dio2, dio3), light-responsive (per2, cry1, adcyap1) and th (tyrosine hydroxylase, involved in dopamine biosynthesis) genes showed significant changes with transition from non-migratory to the migratory state. There were significantly higher mRNA expressions in autumn, except for higher th levels in the spring. Furthermore, the expression patterns of dnmt3a (not dnmt3b) and tet2 genes suggested an epigenetic difference between the non-migrant and migrant periods, and the spring and autumn migrant periods. These results demonstrate for the first time seasonal transition in hypothalamic gene expressions, and suggest differences in regulatory strategies at the transcriptional level for spring and autumn migrations in songbirds.

Bright light at night alters the perception of daylength in Indian weaver bird (Ploceus philippinus).

The brighter nights have posed new challenges to the wild species by affecting their temporal physiology. The present study on Indian weaver bird (Ploceus philippinus) investigated if exposure to bright light at different phases of night affects their clock-mediated daily functions. Birds were placed individually in specially designed activity cages under short days and long nights (8L:16D; L = 100 lux, D < 0.1 lux) for ∼3 weeks (19 days). Thereafter, they were divided into four groups (n = 6-9), and given ∼2 lux light either for the entire night (ZT 08-24; zeitgeber time 0 = time of light on; pattern A) or for 4 hr (pattern B), placed in 16 hr night such that its onset coincides with the onset of night (early night group, ZT 08-12), its end with the end of night (late night group, ZT 20-24), or the night was interrupted in the middle (midnight group, ZT 14-18). The results showed that bright light in entire night induced early onset of day activity and fragmented rest at night, however, if given at different phases of night, it made the days longer by delaying end (early night group) or advancing onset of daily activity (late night group). It also suppressed the melatonin levels and increased body temperature. These results suggest that bright light at night alters the perception of daylength and affects the underlying physiology. The findings may be useful in adopting a strategy for use of night light without disturbing species fitness in their environment.