Plasma characterization of a microwave discharge ion source with mirror magnetic field configuration.

Microwave coupling to plasma through cavity dependent resonant modes is one of the key aspects in a microwave discharge ion source (MDIS) for improving the ion beam qualities as well as plasma dynamics. Knowing these concerns, a MDIS is designed, fabricated, and developed at Institute for Plasma Research to produce high current and a low emittance ion beam for accelerator applications. The present manuscript reports the development of MDIS and the characterization results of the first plasma produced by launching a microwave (MW) of frequency 2.45 GHz. The plasma is characterized at a particular distance away from the ion extraction aperture, using three diagnostics tools such as a Langmuir Probe (LP), Optical Emission Spectroscopy (OES), and a microwave spectrum analyzer. The required mirror-B magnetic field is created by placing four high power ring magnets (each magnet pole strength is 1.38 T) around the cylindrical source chamber. All diagnostics measurements are performed under an operating pressure of range 2 × 10-4-1 × 10-3 mbar and the plasma absorbed power of 30-160 W. The measured cold electron temperature and density varies in the range of ∼1.5-11.8 eV and 5.6 × 1016 m-3-6 × 1017 m-3, respectively, within the source volume. The electron population has distinct hot and cold plasma temperature. The hot electron temperature changes from ∼20 to 70 eV within the above absorbed power range. The LP and OES measurements witnessed the density jumps from under-dense (∼7.3 × 1016 m-3) to over-dense (∼2.9 × 1017 m-3) for the change in absorbed power from 50 W to 80 W. This density jump is accompanied by the sideband generation around the cavity resonant mode (including the launched MW) frequencies which range from 238 kHz to 873 kHz and is recognized as ion waves from the dispersion relation. The ion temperature, estimated from these observed low frequency instabilities, changes from 0.095 to 1.25 eV. The influences of these instabilities on beam emittance growth are of paramount importance in future studies.

Therapeutic effect of ferulic acid, an ethereal fraction of ethanolic extract of seed of Syzygium cumini against streptozotocin-induced diabetes in male rat.

Diabetic therapeutic and antioxidative effects of an ethereal fraction of the ethanolic extract of the seed of Syzygium cumini was studied in streptozotocin (STZ)-induced diabetic rats. Diabetes resulted in a significant elevation in the fasting blood glucose level and in the activity of hepatic glucose-6-phosphatase. There was diminution in the levels of glycogen in the liver and skeletal muscle along with diminution in the activities of hepatic glucose-6-phosphate dehydrogenase, catalase and peroxidase in diabetic rats when compared with controls. Hepatic levels of thiobarbituric acid reactive substance (TBARS) and conjugated dienes (CD) were elevated in respect to control. Oral coadministration of the above fraction to diabetic rats resulted in significant protection in all these parameters. Histological studies of the pancreas showed a qualitative diminution in the area and volume of the islet's of Langerhans, but coadministration of the specific fraction resulted in a significant recovery of the islet's of Langerhans. Chromatography study revealed that the used fraction was ferulic acid (FA). Treatment with FA in normoglycemic rats did not show any significant change in the levels of the selected biosensors. The possible hypothesis for the therapeutic effect of FA against diabetes may be due to its pancreatic beta-cell regenerative effect and/or due to its antioxidant properties.

Lymphocyte proliferation in mice congenitally deficient in T-cell receptor alpha beta + cells.

In mice and humans, T cells are characterized on the basis of T-cell receptor (TcR) expression and divided into the major TcR alpha beta + and minor TcR gamma delta + populations. TcR alpha beta + cells are considered to be the primary regulators of the immune response, whereas the function of TcR gamma delta + cells is unclear. Mice congenitally deficient in TcR alpha beta-expressing cells provide an ideal model for analyzing the independent in vivo function of TcR gamma delta + cells in the absence of TcR alpha beta + cells. Here we report that lymphoid organs in TcR alpha mutant mice undergo substantial enlargement after being challenged by environmental antigens. This organ expansion can be attributed in part to increases in the relative proportions and absolute numbers of TcR gamma delta + cells, but an expansion of the recently described TcR beta + alpha - population also has a role. The expansion of the TcR gamma delta + population is polyclonal, as evidenced by the usage of multiple gamma and delta variable chain segments. Furthermore, a substantial proportion of the cells appears to be activated and these activated cells express surface activation markers. The results clearly demonstrate that TcR gamma delta + cells proliferate independently in response to a broad spectrum of challenges. Moreover, since the expansion of the lymphoid tissues and the TcR gamma delta + cell population is excessive relative to that seen in wild-type animals, one role of TcR alpha beta + cells is directly or indirectly to limit the responses of the other lymphoid components.

Immunoglobulin synthesis and generalized autoimmunity in mice congenitally deficient in alpha beta(+) T cells.

Through cognate B-cell-T-cell interactions and provision of cytokines, CD4+ T-cell antigen receptor (TCR) alpha beta+ T cells regulate immunoglobulin isotype synthesis. Murine IgG1 and IgE secretion is therefore substantially T-cell-dependent, whereas IgM and IgG3 secretion is not. Here we report that in the absence of alpha beta T cells, B cells expand, differentiate and secrete copious amounts of antibodies of 'T-dependent' isotypes. Moreover, the antibodies are reactive towards self-antigens, as in patients with systemic lupus erythematosus, so autoantibodies of 'T-dependent' type can develop without the help of CD4+ alpha beta T cells. This phenotype is not evident in mice or humans that are congenitally deficient in specific alpha beta T-cell functions, but bears comparison with B-cell hyperactivity and autoimmunity in transplant rejection and in immunodeficiencies such as AIDS.

Rearrangement and diversity of T cell receptor beta chain genes in thymocytes: a critical role for the beta chain in development.

Thymocytes from mice congenitally deficient for TCR alpha chain synthesis were examined for the status of their TCR beta chain genes, by DNA sequencing and by a novel technique that analyzes populations of gene rearrangements. TCR beta chain genes were predominantly productively rearranged, in contrast with the statistical prediction for a quasi-random rearrangement process. Therefore, productive TCR beta chain gene rearrangement appears to be a critical step in thymocyte maturation, independent of TCR alpha chain expression. Moreover, the beta chain gene rearrangements in TCR alpha-/-mice are typical of those found in the thymus and periphery of normal mice. Thus, the extent of junctional diversity is a property of TCR genes that is imposed prior to selection on the mature alpha beta TCR.