Early diagnosis of mild cognitive impairment and Alzheimer's with event-related potentials and event-related desynchronization in N-back working memory tasks.

BACKGROUND AND OBJECTIVE: In this study we investigate whether or not event-related potentials (ERP) and/or event-related (de)synchronization (ERD/ERS) can be used to differentiate between 27 healthy elderly (HE), 21 subjects diagnosed with mild cognitive impairment (MCI) and 15 mild Alzheimer's disease (AD) patients. METHODS: Using 32-channel EEG recordings, we measured ERP responses to a three-level (N-back, N = 0,1,2) visual working memory task. We also performed ERD analysis over the same EEG data, dividing the full-band signal into the well-known delta, theta, alpha, beta and gamma bands. Both ERP and ERD analyses were followed by cluster analysis with correction for multicomparisons whenever significant differences were found between groups. RESULTS: Regarding ERP (full-band analysis), our findings have shown both patient groups (MCI and AD) with reduced P450 amplitude (compared to HE controls) in the execution of the non-match 1-back task at many scalp electrodes, chiefly at parietal and centro-parietal areas. However, no significant differences were found between MCI and AD in ERP analysis whatever was the task. As for sub-band analyses, ERD/ERS measures revealed that HE subjects elicited consistently greater alpha ERD responses than MCI and AD patients during the 1-back task in the match condition, with all differences located at frontal, central and occipital regions. Moreover, in the non-match condition, it was possible to distinguish between MCI and AD patients when they were performing the 0-back task, with MCI presenting more desynchronization than AD on the theta band at temporal and fronto-temporal areas. In summary, ERD analyses have revealed themselves more valuable than ERP, since they showed significant differences in all three group comparisons: HE vs. MCI, HE vs. AD, and MCI vs. AD. CONCLUSIONS: Based on these findings, we conclude that ERD responses to working memory (N-back) tasks could be useful not only for early MCI diagnosis or for improved AD diagnosis, but probably also for assessing the likelihood of MCI progression to AD, after further validated by a longitudinal study.

Substrate, moisture, temperature and seed germination of the threatened endemic tree Eriotheca vargasii (Malvaceae) / Sustrato, humedad, temperatura y germinación de semillas del árbol endémico amenazado Eriotheca vargasii (Malvaceae)

Abstract We studied the germination of Eriotheca vargasii (Malvaceae), a poorly known endemic Peruvian Andean tree species characteristic of the dry forests of the Torobamba river valley, Peru. We determined seed characteristics, embryo morphology, viability, and assessed the influence of substrate (natural soil and commercially prepared media), temperature (controlled at 25 ºC and at ambient temperature between 18-22 ºC), and moisture (25 % and 50 % field capacity) on seed germination. Most seeds were ovoid in shape and although they contained well-developed embryos, only 46 % of them were viable. Substrate moisture levels had no influence on germination capacity or rate. In contrast, temperature and substrate type showed strong effects on germination. We observed the highest proportion of germinated seeds in prepared media at both temperatures tested (> 61 %). Furthermore, substrate types also influenced germination rates, with lower values in natural soil. The strongest effect on germination rates was by temperature, enhancing the difference in responses in substrate types (up to 90 % in commercially prepared media at 25 ºC). The low proportion of germinated seeds in soil (< 39 %), together with external local stress factors (e.g. grazing impact by herbivores), may be the critical factors contributing to the nearly total absence of seedlings and saplings of E. vargasiiin the study area despite abundant seed production. In order to ensure a supply of E. vargasii seedlings for reforestation efforts, we recommend producing E. vargasii plants in nurseries and conducting reforestation trials. We suggest that germination of seedlings is done following guidelines from this study. Rev. Biol. Trop. 66(3): 1162-1170. Epub 2018 September 01.

Resumen Estudiamos la germinación de Eriotheca vargasii (Malvaceae), un árbol endémico poco conocido de los andes peruanos y carácterístico de los bosques secos del valle del río Torobamba. Determinamos las características de la semilla, la morfología del embrión y la viabilidad; además evaluamos la influencia del sustrato (suelo y sustrato preparado comercialmente), temperatura (controlada a 25 ºC y sin control entre 18-22 ºC) y humedad (25 % y 50 % de capacidad de campo) sobre la germinación de las semillas. La mayoría de las semillas tuvo forma ovoide y aunque la mayoría contenía embriones bien desarrollados, sólo el 46 % de estas fue viable. Los niveles de humedad del sustrato no tuvieron influencia sobre la capacidad o tasa de germinación, mientras que la temperatura y el tipo de sustrato si tuvieron efectos visibles. Observamos las proporciones más altas de semillas germinadas en los sustratos preparados y en ambas temperaturas (> 61 %). Adicionalmente, el tipo de sustrato también influenció las tasas de germinación, con valores más bajos en el suelo natural. El efecto más fuerte sobre las tasas de germinación se dió por la temperatura, aumentando las diferencias de las respuestas de acuerdo al tipo de sustrato (hasta un 90 % de germinación en sustratos preparados comercialmente y a 25 ºC). La baja proporción de semillas germinadas en el suelo (< 39 %), junto con factores locales de estrés externos (como por ejemplo el impacto por herbivoría), pueden ser elementos clave que están contribuyendo a la casi ausencia total de plántulas de E. vargasii en el área de estudio, a pesar de una producción de semilla abundante. Para asegurar proveer plantones de E. Vargasii para esfuerzos de reforestación, recomendamos producir plantas de esta especie en viveros y realizar experimentos de reforestación. Sugerimos que la germinación de las plántulas se haga siguiendo las recomendaciones de este estudio.