RESUMO
Objective To study the relationship between lipoprotein lipase (LPL) gene Hind Ⅲ and Pvu Ⅱ polymorphisms and type 2 diabetes mellitus (T2DM).Methods Polymerase chain reaction-restriction fragment length polymorphism-(PCR RFLP) technique was used to detect the genotypes of Hind Ⅲ and Pvu Ⅱ polymorphisms in the LPL gene from 110 T2DM patients and 157 healthy controls.Results The frequencies ofgenotype (H-H-,H+H-,H+H+)and allele(H,H+) of Hind Ⅲ between T2DM cases and controls showed no differences (x2 =1.998,1.262,P 0.368,0.261).There were differences in the frequency of genotype P-P-(20.9％ vs.10.8％),P+P-(40.0％ vs.58.6％),P+P+(39.1％ vs.30.6％) of PvuⅡ between T2DM and controls (x2 =10.157,P=0.006).The individuals who carried P+P genotype had an higher morbidity of T2DM than those who carried P P genotype (OR 2.833,95％CI:1.374 5.814).Conclusions The Hind Ⅲ polymorphisms in the LPL gene is not associated with T2DM risk,but the Pvu Ⅱ polymorphisms in the LPL gene is related with T2DM risk in Chinese Han population in Xinjiang,and the P+P- genotype might be a susceptible factor of T2DM.
RESUMO
OBJECTIVE: This study was to investigate the relationship between the 40 bp VNTR polymorphism of dopamine transporter gene (DAT1) and cancer with Uighur medicine abnormal Hilit on Chinese Uighur population of Xinjiang province. METHODS: Divided the cancer patients into four body fluids according to Uighur medicine theory, the polymerase chain reaction and VNTR polymorphism technique was employed to detect genotype and allele frequencies of a 40 bp VNTR polymorphism situated in 3' untranslated region of the DAT1 gene in 47 cancer patients with abnormal black Savda, 26 cancer patients with other abnormal Hilit and 57 normal control subjects in Uighur population of Xinjiang Province. RESULTS: (1) In our sample,the repeat numbers of 40 bp were 6 to 11 (PCR product length of 320 bp to 520 bp) and 10-repeats allele (480 bp) detected was the dominant allele of DAT1 gene polymorphisms with the allele frequency 90.4%. Six kinds of genotype were detected in this study and the genotype 480 bp/480 bp was the most common genotype with genotype frequency 80.7%. (2) The susceptibility to cancer patients with abnormal black Savda among the subjects with 10 repeat (R) allele and 10/10 genotypes and the subjects with non-10 repeat (R) allele and non-10/10 genotypes were similar (P = 0.158, OR = 1.994, 95% CI = 0.754-5.275; P = 0.138, OR = 2.143, 95% CI = 0.772-5.947). No significant differences for the genotype frequency or the allele frequency of the 40 bp VNTR polymorphism of DAT1 were revealed between cancer patients with abnormal black Savda and cancer patients with other abnormal Hilit (P = 0.729, P = 0.782). CONCLUSION: The 40 bp VNTR polymorphism of DAT1 may not be correlated to the susceptibility to cancer with Uighur medicine abnormal Hilit.