RESUMO
(1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this "PARB clade" that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.
RESUMO
La colección de hongos patógenos y simbiontes de insectos y otros artrópodos del Centro de Estudios Parasitológicos y de Vectores es única por sus características específicas en la Argentina y por preservar hongos en cultivo, in vitro e in vivo. Esta colección está abierta para actividades de investigación, docencia, servicios y asesoramiento público y privado. Un total de 421 aislamientos pertenecientes a 23 géneros (Ascomycota: 16, Entomophthoromycotina: 4 Glomeromycota: 2 y Oomycota: 1) son preservados en la colección mediante diversos métodos: criopreservación en freezer a −20 °C y −70 °C, agua destilada estéril, papel, arena y liofilización. Las cepas que integran la colección fueron aisladas de insectos y de otros artrópodos; y a partir de suelo (usando insectos trampa o aislamiento directo en medios selectivos). La identificación se realizó mediante estudios de caracterización morfológicos y, en algunos casos, por taxonomía molecular. Esta colección se utiliza como centro de referencia para identificación de especies, certificaciones, estudios de investigación y docencia, transferencia tecnológica, servicios a terceros, depósito de cepas y preservación de la diversidad y conservación del germoplasma. Las cepas preservadas son, en su mayor parte, nativas de distintas regiones de la Argentina. La colección fue originada en el año 1988 y está registrada en la base de la Federación Latinoamericana de Colecciones de Cultivos y en la World Federation of Culture Collections.
The collection of fungal pathogens and symbionts of insects and other arthropods of the Centro de Estudios Parasitológicos y de Vectores, La Plata, Argentina, is unique because it preserves in vivo and in vitro cultures of fungal pathogens. This culture collection is open for research, teaching, consulting services, and strain deposit. It contains 421 strains belonging to 23 genera (16 Ascomycota, 4 Entomophthoromycotina, 2 Glomeromycota and 1 Oomycota), and the cultures are preserved by different methods such as cryopreservation in freezer at −20 °C and −70 °C, paper, distilled water and lyophilization. Fungi were isolated from insects, other arthropods, and soil (by using insect baits and selective media). Species were identified by morphological features and in a few strains by molecular taxonomy (PCR of rDNA). This collection is a reference center for species identification/certifications, research and teaching purposes, strain deposit, transference and consultancy services, and its overall goal is to preserve the fungal germplasm and ex situ diversity. Most of the strains are native of Argentina. The collection was originated in 1988 and is registered in the Latin American Federation for Culture Collections and in the World Federation of Culture Collections.
Assuntos
Animais , Artrópodes , Ascomicetos , Simbiose , Insetos , Argentina , Preservação Biológica , Artrópodes/microbiologia , Ascomicetos/isolamento & purificação , Insetos/microbiologiaRESUMO
The collection of fungal pathogens and symbionts of insects and other arthropods of the Centro de Estudios Parasitológicos y de Vectores, La Plata, Argentina, is unique because it preserves in vivo and in vitro cultures of fungal pathogens. This culture collection is open for research, teaching, consulting services, and strain deposit. It contains 421 strains belonging to 23 genera (16 Ascomycota, 4 Entomophthoromycotina, 2 Glomeromycota and 1 Oomycota), and the cultures are preserved by different methods such as cryopreservation in freezer at -20°C and -70°C, paper, distilled water and lyophilization. Fungi were isolated from insects, other arthropods, and soil (by using insect baits and selective media). Species were identified by morphological features and in a few strains by molecular taxonomy (PCR of rDNA). This collection is a reference center for species identification/certifications, research and teaching purposes, strain deposit, transference and consultancy services, and its overall goal is to preserve the fungal germplasm and ex situ diversity. Most of the strains are native of Argentina. The collection was originated in 1988 and is registered in the Latin American Federation for Culture Collections and in the World Federation of Culture Collections.
