RESUMO
Сorynebacteria non-diphtheria of С. pseudodiphtheriticum strains, despite the absence of the ability to produce toxin, can be associated with the development of inflammatory diseases of the respiratory and urogenital tract, skin, purulent-septic processes of various localization, etc. This indicates the presence of other pathogenicity factors, in addition to toxin, which may be adhesive and invasive activity. Characteristics of pathogenicity factors (adhesiveness and invasiveness) of Corynebacteria non-diphtheria isolated from patients with pathology of the respiratory tract. The strains of Corynebacteria non-diphtheria (38) isolated from the upper respiratory tract from patients with chronic tonsillitis (C. pseudodiphtheriticum - 9) and angina (C. pseudodiphtheriticum - 14 pcs.), As well as practically healthy subjects (C. pseudodiphtheriticum - 15 pcs.). The ability for adhesion and invasion of corynebacteria was studied on the culture of the cells of the pharyngeal epithelium Hep-2 carcinoma. The number of corynebacteria, adherent and invaded on Hep-2 cells, was determined by sowing the flush with 20% serum agar, followed by counting the average number of colony forming units (CFU) per 1 ml. Electron microscopic investigation of adhesion and invasion of corynebacteria on the culture of Hep-2 cells was carried out by transmission electron microscopy. The adhesiveness of strains of C. pseudodiphtheriticum isolated from practically healthy individuals was lower (p≤0,05) than that of all the investigated strains of Corynebacteria non-diphtheria isolated from patients with pathology of the respiratory tract. The most pronounced adhesive properties (238.3±6.5 CFU/ml) were found in C. pseudodiphtheriticum strains isolated from patients with angina compared with those isolated from patients with chronic tonsillitis. Adhesiveness and invasiveness in all strains studied had a positive correlation. Electron microscopic examination shows corynebacteria, both adherent to the surface of Hep-2 cells and accumulated contrast medium, and invasive, electron-transparent. Corynebacteria non-diphtheria of C. pseudodiphtheriticum strains isolated from patients with respiratory tract pathology (angina, chronic tonsillitis) had a higher ability to adhere and invade than C. dodiphtheriticum strains isolated from practically healthy individuals. The pronounced ability for adhesion and invasion, considered as pathogenicity factors of C. pseudodiphtheriticum, allows them to realize their pathogenic potential, protecting against the action of the host's immune system and antibacterial drugs.
