Multiple Methods of Dentin Translucency for Age Estimation.

Background: Age estimation is an essential part of forensic odontology and many methods are available with variable accuracy. However, the research is going on to find the simple and most reliable method. Objectives: By measuring the length and area of root dentin translucency in extracted tooth specimens manually and digitally, and comparing them, this study aims to find the most reliable technique of age assessment. Materials and Methods: One-hundred and twenty-five single-rooted extracted human permanent teeth were selected for the study. In both unsectioned and sectioned teeth, the total length of root dentin translucency was manually measured using a digital vernier caliper. Then, in a ground-sectioned tooth specimen, the total length and area of root dentin translucency were measured digitally using a scanner scale and computer software. Both the methods were compared. Results: In both manual and digital approaches, the length and area of root dentin translucency grew with age, from younger to older age groups. On comparing the measurements, the correlation coefficient was somewhat higher for digital area measurements than other measurements (r = 0.985). Conclusion: The digital technique outperforms the traditional way for calculating age, and furthermore, measuring the area in the digital method for age estimation has shown to be more accurate.

Correlation Between Histological Grading and Ploidy Status in Oral Leukoplakia, Oral Submucous Fibrosis, and Oral Squamous Cell Carcinoma: A Flow Cytometric Analysis.

BACKGROUND: Methods to analyze progression of carcinogenesis and stage of cancer are vital nowadays due to the high prevalence of these lesions. DNA ploidy analysis is one such important method in early diagnosis and improving prognosis. AIMS AND OBJECTIVES: The aim of this study was to correlate histopathological grading and DNA ploidy in oral leukoplakia, oral submucous fibrosis (OSMF), and oral squamous cell carcinoma (OSCC) cases. MATERIALS AND METHODS: Our study included 80 subjects, grouped into 4 groups of 20 each of OSCC, leukoplakia, OSMF, and healthy individuals. Histopathological grading was carried out in study cases, DNA ploidy was estimated using flow cytometry, and both the findings were correlated. RESULTS: Among the 20 cases of leukoplakia group, 6 cases showed aneuploidy and 14 showed diploidy. In the 20 cases of OSF group, 2 cases showed aneuploidy and 18 showed diploidy, and in the 20 cases of OSCC group, 10 showed aneuploidy and 10 showed diploidy. Most of the aneuploidy cases showed severe dysplasia. CONCLUSION: Analysis of DNA ploidy status can serve as a diagnostic tool for early detection of malignancies owing to the subjective nature of traditional histopathological grading.

Comparison of Cheiloscopy and Rugoscopy in Karnataka, Kerala, and Manipuri Population.

AIMS AND OBJECTIVES: The aim of the study was to evaluate and compare lip prints and palatal rugae pattern in Kerala, Karnataka, and Manipuri population. MATERIALS AND METHODS: The study involved 180 individuals (60 each from Karnataka, Kerala, and Manipuri population). Lipstick was used to record lip prints, which were visualized by magnifying lens. Palatal rugae were recorded on maxillary casts of all subjects and analyzed following Kapali S et al.' s classification. Statistical Package for the Social Sciences version 20 for Windows software was used for analysis. RESULTS: Among the study population, most frequent lip print pattern was Type 3 and least was Type 1'. When patterns were compared between groups, Type 3 was the most common in Manipuri and Kerala and Type 3 in Karnataka groups. In the entire population, males showed Type 3 and females showed Type 1. On analysis of overall rugae wavy, forward and divergence patterns were predominant. On comparison of gender, males demonstrated greater number of wavy and perpendicular rugae, and females had curved, straight, forward, and backward. CONCLUSION: Both cheiloscopy and rugoscopy have the prospective to recognize an individual. Cheiloscopy is more reliable than rugoscopy in making out the group and gender of an individual.

Clinical Utility of Serum Homocysteine and Folate as Tumor Markers in Oral Squamous Cell Carcinoma - A Cross-Sectional Study.

INTRODUCTION: Oral Squamous Cell Carcinoma (OSCC) is a common malignancy involving head and neck. Identifying the markers of molecular levels or biochemical markers involving the various metabolic reactions associated with the initiation and biological behavior of individual tumors are very important in diagnosis and prognosis. AIM: To measure and compare the levels of serum Homocysteine (Hcy) and serum folate in OSCC patients, smoking group and healthy subjects and also to assess the clinical utility of serum Hcy as a potential tumor marker in OSCC. MATERIALS AND METHODS: The study group comprised of 60 subjects, of whom 30 were classified as OSCC cases (GROUP I) and 15 were classified as smokers without OSCC (GROUP II). The control group included 15 healthy individuals without smoking habit (Group III). Hcy was measured with High Performance Liquid Chromatography (HPLC). Folate estimation was done by Chemiluminiscence Immuno Assay (CLIA). Comparison of mean Hcy and folate values among the groups was done using ANOVA with Post-Hoc Games Howell test. Gender was compared using Chi-square test. Comparison of mean age was using ANOVA with Post-Hoc Tukey's test. RESULTS: The mean serum folate level in OSCC patients was 5.34ng/mL, 7.68ng/mL in smoking group and 10.99ng/mL in control group. There was a significant difference in the mean serum folate levels among the three study groups (p<0.001). The mean serum Hcy in OSCC patients was 23.58µmol/L, 17.46µmol/L, in smoking group and 10.76µmol/l in controls. There was a significant difference in the mean serum Hcy levels among the three study groups (p<0.001). CONCLUSION: The present study found an interesting association with serum Hcy and folate levels in OSCC which could be useful as a biochemical "Tumor Marker" and thereby providing insights into the onset and progression of the disease.

Buccal Micronucleus Cytome Assay in Sickle Cell Disease.

INTRODUCTION: Sickle Cell Anaemia (SCA) is a commonly inherited blood disorder preceded by episodes of pain, chronic haemolytic anaemia and severe infections. The underlying phenomenon which causes this disease is the point mutation in the haemoglobin beta gene (Hbß) found on chromosome 11 p. Increased oxidative stress leads to DNA damage. DNA damage occurring in such conditions can be studied by the buccal micronucleus cytome assay, which is a minimally invasive method for studying chromosomal instability, cell death and regenerative potential of human buccal tissue. AIM: To evaluate genomic instability in patients with sickle cell disease by buccal micronucleus cytome assay. MATERIALS AND METHODS: The study included 40 sickle cell anemia patients (Group A) and 40 age and sex matched controls (Group B). Buccal swabs were collected and stained with Papanicolaou (PAP). Number of cells with micronucleus, binuclei, nuclear bud, pyknosis and karyolysis were counted in two groups as parameters for the evaluation of genome stability. RESULTS: All the analysis was done using t-test. A p-value of <0.001 was considered statistically significant. There was a statistically significant increase in micronuclei number in SCA patients when compared with controls. Karyolytic (un-nucleated) cell number in Group A was more than to those of the controls. CONCLUSION: The results might suggest that patients with sickle cell anaemia have genome instability which is represented by the presence of micronuclei in the somatic cells. Presence of apoptotic cells might only indicate the bodily damage to the tissue as a result of the disease.

Ameloblastic fibroma of the maxilla with bilateral presentation: report of a rare case with review of the literature.

Ameloblastic fibroma (AF) is an uncommon benign odontogenic tumour, with both epithelial and mesenchymal neoplastic proliferation. It occurs most frequently in the posterior region of the mandible, while its occurrence in the maxilla is extremely rare. They are usually encountered in children, emphasizing it as an important diagnostic consideration. Herein, we report the first case of a bilateral maxillary ameloblastic fibroma in a 2-year-old female child patient who presented with a chief complaint of swelling in the right mid facial region.