RESUMO
Dengue fever is a viral mosquito borne disease transmitted by day biting mosquito, Aedes aegypti. No medicine has been proven to be effective for the complete cure of dengue and mosquito control remains to be the only effective option. Increased cases of dengue contraction are being enormously reported worldwide every year. Thus, the urge for an effective measure remains a factor of major concern. In the present study, biosynthesized spherical-like structured zinc oxide (ZnO) nanoparticles using Indigofera tinctoria leaf extracts are employed as a mosquito controlling agent. The biosynthesized nanoparticles are characterized by UV-Vis, FTIR, FESEM, EDAX, XRD, Zeta Potential, and DLS analysis. The efficacy of the green synthesized ZnO nanoparticles were tested against different larval and pupal stages of A. aegypti. Further, it is established that a significant LC50 values of 4.030 ppm in first instar and 7.213 ppm in pupae of A. aegypti is due to the impact of synthesized ZnO. Histological studies confirmed that effective and destructive changes were observed in larval body tissues particularly in the fat cells and the midgut. Therefore, this study highlights the application of biosynthesized ZnO nanoparticles as a potential candidate for safe and eco-friendly agent against the dengue vector, A. aegypti.
