RESUMO
Impact of choline alphoscerate on morphological hepatic indices in rats with hepatic cirrhosis and hepatic encephalopathy, caused by injection of 15% solution of CCl(4) during 4 weeks, in comparison to standard scheme of treatment, was studied. Reduction of the hepatic histological changes severity, of the injured hepatocytes volume, of stromalparenchymal ratio and enhancement of nucleocytoplasmic ratio in rats with hepatic cirrhosis under the choline alphoscerate impact were noted. The fibrosis and histological activity index in accordance to Knodell's scale witnessed a reduction of severity of hepatic affection in rats, which were treated, using choline alphoscerate.
Assuntos
Glicerilfosforilcolina/farmacologia , Encefalopatia Hepática/tratamento farmacológico , Cirrose Hepática/tratamento farmacológico , Fígado/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Animais , Tetracloreto de Carbono , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/ultraestrutura , Tamanho Celular , Citoplasma/efeitos dos fármacos , Citoplasma/ultraestrutura , Dipeptídeos/farmacologia , Encefalopatia Hepática/induzido quimicamente , Encefalopatia Hepática/patologia , Hepatócitos/efeitos dos fármacos , Hepatócitos/patologia , Hepatócitos/ultraestrutura , Histocitoquímica , Lactulose/farmacologia , Fígado/patologia , Fígado/ultraestrutura , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/patologia , Masculino , Ratos , Rifamicinas/farmacologia , RifaximinaRESUMO
Connection of the cytokins profile with experimental hepatic encephalopathy (HE) in rats was studied. Investigation was conducted on 20 laboratory rats, in which HE was simulated, using СCl4 injection. The Ñnterleukins (ÐL) level, including, ÐL1ß, ÐL4, ÐL10 and interferonγ(IFNγ), was determined using immunoassay method with the help of polyclonal antibodies. Enhancement of the proinflammatory ÐL1ß and ÐÐФγ content in the rats blood serum while induced HE by 57.9 and 39.5% accordingly (p <0.05), comparing with such in a control and the compensation enhancement of the anti'inflammatory IL4 and IL10 level by 34.6 and 75.9% (p < 0.05) was established. The results obtained confirms the cytokins role in pathogenesis of HE, determination of their level constitutes significant criterion for the posttransplantation complications prog' nostication. Inflammation and profile of cytokins constitute the main target in therapy of HE in patients, suffering liver cirrhosis