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1.
Toxins (Basel) ; 16(1)2024 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-38251240

RESUMO

Vip3Aa, secreted by Bacillus thuringiensis, is effective at controlling major agricultural pests such as Spodoptera frugiperda. However, to control Vip3Aa resistance evolved in the field by different lepidoptera species, an in-depth study of sequence--structure--activity relationships is necessary to design new Vip3Aa variants. In this study, the four specific loops (ß4-ß5 loop, ß9-ß10 loop, ß12-ß13 loop, and ß14-ß15 loop) in domain III were selected and four loop mutants were constructed by replacing all residues in each specific loop with alanine. We obtained soluble proteins for three of the loop mutants, excluding the ß9-ß10 loop. These loop mutants have been characterized by toxicity bioassays against S. frugiperda, proteolytic processing, and receptor binding. These results indicate that the ß4-ß5 loop and ß14-ß15 loop are involved in receptor binding and Vip3Aa toxicity. Based on this, we constructed numerous mutants and obtained three single mutants (Vip3Aa-S366T, Vip3Aa-S366L, and Vip3Aa-R501A) that exhibited significantly increased toxicity of 2.61-fold, 3.39-fold, and 2.51-fold, respectively. Compared to Vip3Aa, the receptor affinity of Vip3Aa-S366T and Vip3Aa-S366L was significantly enhanced. Furthermore, we also analyzed and aligned the three-dimensional structures of the mutants and Vip3Aa. In summary, these results indicate that the loops in domain III have the potential to be targeted to enhance the insecticidal toxicity of the Vip3Aa protein.


Assuntos
Bacillus thuringiensis , Animais , Bacillus thuringiensis/genética , Agricultura , Alanina , Bioensaio , Spodoptera
2.
Foods ; 12(22)2023 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-38002131

RESUMO

Edible insects are a highly nutritious source of protein and are enjoyed by people all over the world. Insects contain various other nutrients and beneficial compounds, such as lipids, vitamins and minerals, chitin, phenolic compounds, and antimicrobial peptides, which contribute to good health. The practice of insect farming is far more resource-efficient compared to traditional agriculture and animal husbandry, requiring less land, energy, and water, and resulting in a significantly lower carbon footprint. In fact, insects are 12 to 25 times more efficient than animals in converting low-protein feed into protein. When it comes to protein production per unit area, insect farming only requires about one-eighth of the land needed for beef production. Moreover, insect farming generates minimal waste, as insects can consume food and biomass that would otherwise go to waste, contributing to a circular economy that promotes resource recycling and reuse. Insects can be fed with agricultural waste, such as unused plant stems and food scraps. Additionally, the excrement produced by insects can be used as fertilizer for crops, completing the circular chain. Despite the undeniable sustainability and nutritional benefits of consuming insects, widespread acceptance of incorporating insects into our daily diets still has a long way to go. This paper provides a comprehensive overview of the nutritional value of edible insects, the development of farming and processing technologies, and the problems faced in the marketing of edible insect products and insect foods to improve the reference for how people choose edible insects.

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