LncRNA AL161431.1 predicts prognosis and drug response in head and neck squamous cell carcinoma.

Background: Long non-coding RNAs (lncRNAs) are increasingly recognized as essential players in various biological processes due to their interactions with DNA, RNA, and protein. Emerging studies have demonstrated lncRNAs as prognostic biomarkers in multiple cancers. However, the prognostic effect of lncRNA AL161431.1 in head and neck squamous cell carcinoma (HNSCC) patients has not been reported. Methods: In the present study, we conducted a series of analyses to identify and validate the prognostic value of lncRNA AL161431.1 in HNSCC, which included differential lncRNAs screening, survival analysis, Cox regression analysis, time ROCanalysis, nomogram prediction, enrichment analysis, tumor infiltration of immune cells, drug sensitivity analysis, and quantitative real-time polymerase chain reaction (qRT-PCR). Results: In this study, we performed a comprehensive survival and predictive analysis and demonstrated that AL161431.1 was an independent prognostic factor of HNSCC, for which a high AL161431.1 level indicated poor survival in HNSCC. Functional enrichment analyses found that cell growth and immune-related pathways were significantly enriched in HNSCC, suggesting that AL161431.1 may play a role in tumor development and tumor microenvironment (TME). AL161431.1-related immune cells infiltration analysis demonstrated that AL161431.1 expression is significantly positively associated with M0 macrophages in HNSCC (P<0.001). Using "OncoPredict", we recognized chemotherapy drugs sensitive to the high expression group. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to identify the expression level of AL161431.1 in HNSCC, and the results further validated our findings. Conclusions: Our findings suggest that AL161431.1 is a reliable prognostic marker for HNSCC and can potentially be an effective therapeutic target.

Analysis of tunable Faraday rotation angle produced by 1D photonic crystals doped with InSb in the terahertz regime.

Simple periodic one-dimensional (1D) common photonic crystals (PCs) doped with InSb are proposed to research the Faraday rotation (FR) effect by applying a 4×4 transfer matrix method. Analysis indicates that the given 1D PCs can realize a giant FR angle. The influences of the properties of InSb (magnetic induction intensity, temperature, and the length of the InSb layer) and the repeat number of the structure on the FR are investigated due to the tunability of InSb and the features of the structure. Through calculation, it is found that, by adjusting these parameters, we can clearly observe the movement of the extreme values of the FR angle. In addition, the numerical results show that when the magnetic induction intensity and the repeat number of the structure are changed, the FR angle will be significantly altered at the fixed extreme frequency point. Specifically, if the temperature and the thickness of InSb layer are altered, the extreme value of the FR and the frequency point where it happens also will change. We believe these obtained results can provide ideas to design optical isolators and optical switches.

Switchable multifunctional modulator realized by the stacked graphene-based hyperbolic metamaterial unit cells.

A tunable multifunctional modulator of the stacked graphene-based hyperbolic metamaterial (HM) cells is proposed. The dielectric constant and group index of HM are theoretically investigated. The calculated results show that, for the cell structure, a transmission window in the reflection zone (TWRZ) can be obtained at the normal incidence, but all reflections are converted to the transmission when the incident angle is near 82°. Concurrently, a single frequency absorption in the transmission zone (SFATZ) is realized, which can be adjusted by the chemical potential of graphene. For the whole structure composed of cell structures with different chemical potentials, the ultra-wideband absorption and transmission window in the absorption zone (TWAZ) can be achieved, which can work in different frequency bands if the given structural parameters can be tailored. Those computed results can apply for switchable frequency-dependent and angle-dependent reflection-transmission modulations, single frequency and ultra-wideband absorbers, and a logic switch based on the TWAZ.

Succinate Modulates Intestinal Barrier Function and Inflammation Response in Pigs.

Succinate is a metabolic intermediate of the tricarboxylic acid (TCA) cycle in all aerobic organisms, and is also a vital microbial metabolite in the gut. Although succinate is known to regulate intestinal metabolism and immune function, its role in the protection of the intestinal epithelial barrier function and inflammation is poorly characterized. In this study, we evaluated the effects of succinate on intestinal epithelial barrier function and inflammation in pigs. Twenty-four growing pigs were distributed into three groups (n = 8) and received either a basal diet (control group) or the same diet supplemented with 0.1% succinate or 1% succinate. The diet supplemented with 1% succinate led to alterations in the intestinal morphology. We confirmed in vitro that 5 mM succinate treatment modulated intestinal epithelial permeability by increased transepithelial electrical resistance (TEER) in intestinal porcine epithelial cell (IPEC)-J2 cells. Furthermore, succinate treatment increased the abundance of tight junction proteins claudin-1, zona occluden (ZO)-1, and ZO-2 in the jejunum in vivo and in vitro. In addition, dietary succinate supplementation promoted the expression of inflammatory cytokines interleukin (IL)-25, IL-10, IL-8, and IL-18 in the jejunum. Taken together, these data identify a novel role of succinate in the modulation of intestinal epithelial barrier function, which may be a nutritional target to improve gut health in animals.