RESUMO
Objective To study the effect of microRNA-204 (miR-204) on the biological characteristics of breast cancer cells.Methods Real-time PCR was used to detect the expression of miR-204 in human breast cancer cell MDA-MB-231 after transfection of miR-204 mimics and inhibitor for 48 h.Flow cytometry was used to analyse the effect of miR-204 on the proliferation and apoptosis of MDA-MB-231 cells.The effect of miR-204 on the migration of MDA-MB-231 cells was detected by Transwell migration assay.Results Real-time PCR analysis showed that miR-204 mimics and inhibitors had significant effect compared with normal control group(P<0.01).Flow cytometry analysis showed that compared with normal control group,the number of G1 phase cells of miR-204 mimics group was significantly decreased(P<0.01),while the number of G2/M cells of miR-204 mimics group was significantly increased(P<0.01).In contrast,the number of G1 phase cells of miR-204 inhibitor group was significantly increased(P<0.01),while the number of G2/M cells of miR-204 inhibitor group was significantly decreased(P<0.01).miR-204 mimics group significantly promoted apoptosis,while the inhibitor group significantly inhibited apoptosis(P<0.01).Transwell migration analysis showed that the number of cells of miR-204 mimics group were significantly reduced,while the number of cells was significantly increased in the inhibitor group(P<0.01).Conclusion We find miR-204,which can promote cell apoptosis and inhibit cell proliferation and migration,is a negative factor in the breast cancer cell line MDA-MB-231.
RESUMO
Full-length cDNA of the Endothelial Differentiation-related Factor-1 (EDF-1) of Gekkojaponicus was obtained by the method of rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). EDF-1 is 1025 bp in length and contains an open reading frame (ORF) corresponding to a deduced protein of 148 amino acids. The molecular mass of the predicted protein is 16.28 kDa and its theoretical isoelectric point is 9.87. Its amino acid sequence shares 57-93% similarity with EDF-1 of a number of other species. Gecko EDF-1 is expressed in most gecko tissues examined including the brain, spinal cord, liver, and kidney, with higher levels in the last two. In situ hybridization (ISH) revealed that positive hybridization signals were present in both the gray and white matter of the spinal cord.
