Can oestrogenic activity in air contribute to the overall body burden of endocrine disruptors?

Endocrine disruptors (EDCs) are emerging contaminants that are harmful to health. Human exposure occurs mainly through ingestion or dermal contact, but inhalation could be an additional exposure route; therefore, this study was conducted to evaluate the oestrogenic activity of airborne particulate matter (PM). Outdoor PM was collected for a year in five Italian sites and extracted with organic solvents (four seasonal extracts/site). The oestrogenic activity was assessed using a gene reporter assay (MELN), and the risk to human health through inhalation was quantified using the results. Moreover, extracts were analysed to assess cytotoxicity (WST-1 and LDH assays) on human bronchial cells (BEAS-2B). The extracts induced a significant cytotoxicity and oestrogenic activity. Oestrogenic activity showed a seasonal trend and was correlated with concentrations of benzo(a)pyrene and toxic equivalency factor. Although a low inhalation cancer risk was found, this study confirmed that oestrogenic activity in air could contribute to overall health risks due to EDC exposure.

Biological effects of particulate matter samples during the COVID-19 pandemic: a comparison with the pre-lockdown period in Northwest Italy.

In 2020, during the COVID-19 pandemic, containment measures were applied inducing potential changes in air pollutant concentrations and thus in air toxicity. This study evaluates the role of restrictions on biological effects of particulate matter (PM) in different Northwest Italy sites: urban background, urban traffic, rural, and incinerator. Daily PM samples collected in 2020 were pooled according to restrictions: January/February (no restrictions), March and April (first lockdown), May/June and July/August/September (low restrictions), October/November/December (second lockdown). The 2019 samples (pre-pandemic period) were pooled as 2020 for comparison. Pools were extracted with organic solvents and extracts were tested to assess cytotoxicity (WST-1 assay) and genotoxicity (comet assay) on BEAS-2B cells, mutagenicity (Ames test) on TA98 and TA100 Salmonella typhimurium strains, and estrogenic activity (gene reporter assay) on MELN cells. Pollutant concentrations were also analyzed (PM10, PM2.5, polycyclic aromatic hydrocarbons). No difference was observed for PM and polycyclic aromatic hydrocarbon concentrations between 2020 and 2019. During lockdown months (2020), PM cytotoxicity/genotoxicity was significantly lower in some sites than during 2019, while considering PM mutagenicity/estrogenic activity some differences were detected but without statistical significance. PM extract effects decreased in some sites during 2020; this may be due to lockdowns that reduced/modified pollutant emissions and may be related also to complex PM origin/formation and to meteorological conditions. In conclusion, the study confirms that PM biological effects cannot be assessed considering only the PM concentration and suggests to include a battery of bioassay for air quality monitoring in order to protect human health from air pollution effects. Supplementary Information: The online version contains supplementary material available at 10.1007/s11869-023-01381-6.

In Vitro Effects of Particulate Matter Associated with a Wildland Fire in the North-West of Italy.

Wildland fires, increasing in recent decades in the Mediterranean region due to climate change, can contribute to PM levels and composition. This study aimed to investigate biological effects of PM2.5 (Ø < 2.5 µm) and PM10 (Ø < 10 µm) collected near a fire occurred in the North-West of Italy in 2017 and in three other areas (urban and rural areas). Organic extracts were assessed for mutagenicity using Ames test (TA98 and TA100 strains), cell viability (WST-1 and LDH assays) and genotoxicity (Comet assay) with human bronchial cells (BEAS-2B) and estrogenic activity using a gene reporter assay (MELN cells). In all sites, high levels of PM10 and PM2.5 were measured during the fire suggesting that near and distant sites were influenced by fire pollutants. The PM10 and PM2.5 extracts induced a significant mutagenicity in all sites and the mutagenic effect was increased with respect to historical data. All extracts induced a slight increase of the estrogenic activity but a possible antagonistic activity of PM samples collected near fire was observed. No cytotoxicity or DNA damage was detected. Results confirm that fires could be relevant for human health, since they can worsen the air quality increasing PM concentrations, mutagenic and estrogenic effects.

The North-western Italy air quality monitoring network: Improving experience of PM2.5 assessment with mutagenicity assay.

The finest fraction of Particulate Matter (PM2.5) carries a large number of pollutants, some of which are assessed as genotoxic, such as some Polycyclic Aromatic Hydrocarbons (PAHs). In many countries, PM2.5 in combination with some PAHs are monitored to assess the concentrations of pollutants, while the air quality is rarely assessed by means of biological assays. Epidemiological studies have demonstrated a significant correlation between these two pollutants and human adverse effects, in particular on the respiratory system. Nevertheless, other air pollutants can induce a biological effect and the cumulative effect of the PM2.5 complex mixture may not be easily deduced by PM2.5 and PAH levels. This study aimed to combine the legislative monitoring of PM2.5 with the study of its mutagenicity. During a full year, daily air samples were collected in nine sites of the North-western Italy air quality monitoring network (Piedmont Region) and PM2.5 and PAH concentrations were assessed. Monthly pooled organic extracts were tested with the Salmonella assay using TA98 and TA100 strains, with and without metabolic activation (±S9), and using TA98NR and YG1021 strains. In all sites, a positive response was observed for TA98 and TA100 especially without S9. A significant mutagenic seasonal variation was detected, with higher mutagenicity in winter and lower responses in summer (average total mutagenicity ratio 27:1). The response of TA98NR and YG1021 compared with TA98 suggested a significant contribution of nitro-compounds to the mutagenicity. No significant differences were found between urban background and rural sites denoting the spread of pollution. A mutagenicity increase, 1.28 Total Mutagenicity Factor/20 m3, was observed for each PM2.5 µg increment. PAH levels and corresponding Toxic Equivalent Factors were highly correlated to mutagenicity results. This work confirms that complex environmental mixtures can be appropriately assessed through the implementation of physical-chemical analyzes with bioassays able to evaluate synergistic and antagonistic effects, especially for highest and lowest pollution settings.