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1.
Am J Ind Med ; 24(4): 365-74, 1993 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-8250057

RESUMO

Inhalation of dust from contaminated organic materials may result in acute respiratory tract illness. Possible mechanisms include toxic and cellular reactions to microbial and other organic products or immunologic responses after prior sensitization to an antigen. A case is presented of a 52 year old male who developed fever, myalgia, and marked dyspnea 12 hr after shoveling composted wood chips and leaves. Inspiratory crackles, hypoxemia, and bilateral patchy pulmonary infiltrates were seen. Precipitating antibody tests for the usual antigens were inconclusive. He improved over 3 days. In order to assess the environmental conditions the patient had experienced, we returned to the site to reproduce and measure respiratory exposures during hand loading of the compost. Visible clouds of fine particulate were easily generated during handling activities. Microscopic examination of these dusts indicated a predominance of spores. Endotoxin concentrations from inspirable and respirable dust samples ranged from 636 to 16,300 endotoxin units/m3. Levels of contaminants found were consistent with those associated with respiratory illness in other agricultural settings. Two respiratory disorders, hypersensitivity pneumonitis (HP) and organic dust toxic syndrome (ODTS), may occur after exposure to organic dusts containing fungal spores and endotoxins. Despite extensive clinical and environmental investigations, we were unable to differentiate these two disorders, and suggest they may represent parts of a spectrum of responses to complex organic dusts, rather than completely distinct clinical entities.


Assuntos
Poeira/efeitos adversos , Doenças Profissionais/etiologia , Doenças Respiratórias/etiologia , Agricultura , Alveolite Alérgica Extrínseca/etiologia , Alveolite Alérgica Extrínseca/microbiologia , Poeira/análise , Endotoxinas/análise , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/microbiologia , Exposição Ocupacional , Doenças Respiratórias/microbiologia , Síndrome , Madeira
2.
J Bioenerg Biomembr ; 23(4): 665-78, 1991 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-1655723

RESUMO

The cytochrome bc1 complex was isolated from yeast mitochondria solubilized with the detergent dodecyl maltoside and reconstituted into proteoliposomes to measure electrogenic proton pumping. Optimal respiratory control ratios of 4.0, obtained after addition of the uncoupler CCCP, and H+/e- ratios of 1.6 were obtained when the proteoliposomes were prepared with egg yolk phosphatidylcholine supplemented with cardiolipin. Moreover, it was critical to remove excess dodecyl maltoside in the final concentrated preparation prior to reconstitution to prevent loss of enzymatic activity. The rate of electrogenic proton pumping, the respiratory control ratios, and the H+/e- ratios were decreased by incubation of the cytochrome bc1 complex with dicyclohexylcarbodiimide (DCCD) in a time and concentration dependent manner. Maximum inhibitions were observed when 50 nmol DCCD per nmol of cytochrome b were incubated for 30 min at 12 degrees C with the intact cytochrome bc1 complex. Under these same conditions maximum labeling of cytochrome b with [14C] DCCD was reported in a previous study [Beattie et al. (1984). J. Biol. Chem. 259, 10562-10532] consistent with a role for cytochrome b in electrogenic proton movements.


Assuntos
Dicicloexilcarbodi-Imida/farmacologia , Complexo III da Cadeia de Transporte de Elétrons/antagonistas & inibidores , Lipossomos/metabolismo , Mitocôndrias/enzimologia , Proteolipídeos/metabolismo , Relação Dose-Resposta a Droga , Eletroforese em Gel de Poliacrilamida , Cinética , Prótons , Leveduras/enzimologia
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