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1.
Artigo em Inglês | MEDLINE | ID: mdl-30425971

RESUMO

Background: Community-acquired pneumonia (CAP) is a leading cause of hospitalization and mortality in children. Diagnosis remains challenging and there are no reliable tools to objectively risk stratify patients or predict clinical outcomes. Molecular distance to health (MDTH) is a genomic score that measures the global perturbation of the transcriptional profile and may help classify patients by disease severity. We evaluated the value of MDTH to assess disease severity in children hospitalized with CAP. Methods: Children hospitalized with CAP and matched healthy controls were enrolled in a prospective observational study. Blood samples were obtained for transcriptome analyses within 24 h of hospitalization. MDTH scores were calculated to assess disease severity and correlated with laboratory markers, such as white blood cell count, c-reactive protein (CRP), and procalcitonin (PCT), and clinical outcomes, including duration of fever and duration of hospitalization (LOS). Univariate and multivariable logistic regression were applied to assess factors associated with LOS and duration of fever after hospitalization. Results: Among children hospitalized with CAP (n = 152), pyogenic bacteria (PB) were detected in 16 (11%), Mycoplasma pneumoniae was detected in 41 (28%), respiratory viruses (RV) alone were detected in 78 (51%), and no pathogen was detected in 17 (11%) children. Statistical group comparisons identified 6,726 genes differentially expressed in patients with CAP vs. healthy controls (n = 39). Children with confirmed PB had higher MDTH scores than those with RV (p < 0.05) or M. pneumoniae (p < 0.01) detected alone. CRP (r = 0.39, p < 0.0001), PCT (r = 0.39, p < 0.0001), and MDTHs (r = 0.24, p < 0.01) correlated with duration of fever, while only MDTHs correlated with LOS (r = 0.33, p < 0.0001). Unadjusted analyses showed that both higher CRP and MDTHs were associated with longer LOS (OR 1.04 [1-1.07] and 1.12 [1.04-1.20], respectively), however, only MDTH remained significant when adjusting for other covariates (aOR 1.11 [1.01-1.22]). Conclusions: In children hospitalized with CAP MDTH score measured within 24 h of admission was independently associated with longer duration of hospitalization, regardless of the pathogen detected. This suggests that transcriptional biomarkers may represent a promising approach to assess disease severity in children with CAP.


Assuntos
Infecções Comunitárias Adquiridas/diagnóstico , Infecções Comunitárias Adquiridas/patologia , Perfilação da Expressão Gênica/métodos , Técnicas de Diagnóstico Molecular/métodos , Pneumonia/diagnóstico , Pneumonia/patologia , Índice de Gravidade de Doença , Adolescente , Criança , Pré-Escolar , Feminino , Hospitalização , Humanos , Lactente , Masculino , Prognóstico , Estudos Prospectivos , Transcriptoma
2.
J Infect ; 71(4): 458-69, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26149186

RESUMO

OBJECTIVES: Animal studies suggest that RSV increases nasopharyngeal (NP) bacterial colonization facilitating bacterial infections. We investigated the influence of antibiotic treatment and colonization with potentially pathogenic bacteria on inflammatory markers and disease severity in RSV-infected in infants. METHODS: Healthy young infants hospitalized with RSV bronchiolitis (n = 136) and age-matched healthy controls (n = 23) were enrolled and NP samples cultured for potentially pathogenic bacteria including: Gram-positive bacteria (GPB): Staphylococcus aureus, Streptococcus pneumoniae, ß-hemolytic Streptococcus; and Gram-negative bacteria (GNB): Moraxella catarrhalis and Haemophilus influenzae. Clinical parameters and plasma IL-8, IL-6 and TNF-α concentrations were compared according to the bacterial class and antibiotic treatment. RESULTS: Antibiotic treatment decreased by 10-fold NP bacterial recovery. Eighty-one percent of RSV infants who did not receive antibiotics before sample collection were colonized with pathogenic bacteria. Overall, GNB were identified in 21% of patients versus 4% of controls who were mostly colonized with GPB. Additionally, in RSV patients NP white blood cell counts (p = 0.026), and blood neutrophils (p = 0.02) were higher in those colonized with potentially pathogenic bacteria versus respiratory flora. RSV patients colonized with GNB had higher plasma IL-8 (p = 0.01) and IL-6 (p < 0.01) concentrations than controls, and required longer duration of oxygen (p = 0.049). CONCLUSIONS: Infants with RSV bronchiolitis colonized with potentially pathogenic bacteria had increased numbers of mucosal and systemic inflammatory cells. Specifically, colonization with GNB was associated with higher concentrations of proinflammatory cytokines and a trend towards increased disease severity.


Assuntos
Antibacterianos/uso terapêutico , Bronquiolite Viral/fisiopatologia , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Nasofaringe/microbiologia , Infecções por Vírus Respiratório Sincicial/fisiopatologia , Carga Bacteriana/efeitos dos fármacos , Biomarcadores , Bronquiolite Viral/virologia , Citocinas/sangue , Feminino , Haemophilus influenzae/isolamento & purificação , Hospitalização/estatística & dados numéricos , Humanos , Lactente , Masculino , Moraxella catarrhalis/isolamento & purificação , Estudos Prospectivos , Infecções por Vírus Respiratório Sincicial/virologia , Índice de Gravidade de Doença , Staphylococcus aureus/isolamento & purificação , Streptococcus/isolamento & purificação , Streptococcus/patogenicidade , Streptococcus pneumoniae/isolamento & purificação
3.
J Clin Microbiol ; 52(2): 674-7, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24478511

RESUMO

The differentiation of Bordetella species, particularly those causing human infection, is problematic. We found that sequence analysis of an internal fragment of nrdA allowed differentiation of the currently named Bordetella species. Analysis of 107 "Bordetella" isolates recovered almost exclusively from human respiratory tract specimens identified several putative novel species.


Assuntos
Bordetella/classificação , Bordetella/genética , DNA Bacteriano/genética , Análise de Sequência de DNA , Bordetella/isolamento & purificação , Infecções por Bordetella/microbiologia , DNA Bacteriano/química , Humanos , Dados de Sequência Molecular , Infecções Respiratórias/microbiologia , Ribonucleosídeo Difosfato Redutase/genética
4.
Pediatr Infect Dis J ; 33(7): e162-7, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24445823

RESUMO

BACKGROUND: An increase in laboratory diagnosis of pertussis was noted in central Ohio during 2010. Diagnosis was made using a polymerase chain reaction assay targeting the multicopy insertion sequence IS481, which is found in both Bordetella pertussis (Bp) and Bordetella holmesii (Bh). An increase in specimens testing positive for Bordetella parapertussis (Bpp) using insertion sequence IS1001 was also noted. METHODS: Nasopharyngeal swab specimens submitted April 1, 2010, to March 31, 2011, were tested using a multiplex polymerase chain reaction assay for Bp/Bh (IS481) and Bpp followed by singleplex assays for Bp and Bh. A subgroup of specimens was also cultured for Bordetella species, and antimicrobial susceptibility testing was performed on recovered organisms. Demographic and clinical features were compared for patients with Bp, Bh and Bpp. RESULTS: Of 520 IS481-positive specimens, 214 (41.1%) were positive for Bp, 79 (15.2%) were positive for Bh and 5 (1.0%) were positive for both Bp and Bh; 222 (42.7%) were negative for both targets. An additional 220 specimens were positive for Bpp. Among a sample of 155 IS481-positive specimens, 40, 15 and 0 were culture positive for Bp, Bh and Bpp, respectively. Among a sample of 55 BparaIS1001-positive (Bpp) specimens, 22, 0 and 0 were culture positive for Bpp, Bp and Bh, respectively. All Bordetella species were susceptible to macrolide antibiotics. Patients with Bh were older than patients with Bp, who were older than those positive for Bpp (mean ages: 12.0, 8.0 and 4.2 years, respectively; P < 0.001). One or more classic signs of pertussis (ie, paroxysmal cough, whoop, post-tussive emesis) were seen in 55.9% of 263 patients (59 Bp, 24 Bh, 80 Bpp and 100 negative for Bordetella species), but did not differ statistically among the groups (χ = 5.1, P = 0.17). CONCLUSIONS: All 3 Bordetella species, Bp, Bh and Bpp, were detected during on outbreak of pertussis-like cough illness. There were noted differences in age and seasonality, but clinical features at the time of presentation did not allow clear differentiation of these infections. All Bordetella species recovered from culture and tested were susceptible in vitro to macrolide antibiotics. Additional study is necessary to further characterize epidemiologic and clinical characteristics of Bh-associated cough illness and to determine potential co-occurrence of Bordetella species with other bacterial and viral respiratory tract pathogens.


Assuntos
Infecções por Bordetella/epidemiologia , Bordetella/classificação , Bordetella/isolamento & purificação , Tosse/epidemiologia , Surtos de Doenças , Adolescente , Antibacterianos/farmacologia , Bordetella/efeitos dos fármacos , Infecções por Bordetella/microbiologia , Criança , Pré-Escolar , Tosse/microbiologia , Elementos de DNA Transponíveis , Feminino , Humanos , Lactente , Macrolídeos/farmacologia , Masculino , Testes de Sensibilidade Microbiana , Reação em Cadeia da Polimerase Multiplex , Nasofaringe/microbiologia , Ohio/epidemiologia , Adulto Jovem
5.
J Clin Microbiol ; 51(12): 4001-7, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24048539

RESUMO

Enteric illness affects millions of individuals annually in the United States and results in >50,000 hospitalizations. The rapid and accurate identification of bacterial pathogens associated with gastroenteritis can aid acute patient management decisions, including the use of antibiotic therapy and infection control. This study compared the ProGastro SSCS multiplex real-time PCR assay (Gen-Probe Prodesse, San Diego, CA) to culture for the identification of Campylobacter spp. (Campylobacter jejuni and Campylobacter coli), Salmonella spp., and Shigella spp. and to broth enrichment followed by an FDA-cleared enzyme immunoassay (EIA) for the identification of Shiga toxin-producing Escherichia coli (STEC) isolates in stool specimens. Stool samples submitted in preservatives for routine culture and EIA were prospectively enrolled and tested at four clinical centers. Discrepancies between the ProGastro SSCS assay and culture or EIA were resolved using bidirectional sequencing. The overall prevalence of the pathogens as detected by culture was 5.6% (1.8% Campylobacter, 1.8% Salmonella, 1.3% Shigella, and 0.8% STEC). When results based on the ProGastro SSCS assay and bidirectional sequencing were applied, the overall prevalence increased to 8.3% (2.3% Campylobacter, 2.6% Salmonella, 1.8% Shigella, and 1.6% STEC). Following resolution of the discrepant results, the sensitivity of the ProGastro SSCS assay was 100% for all pathogens, and the specificities ranged from 99.4% to 100%. The sensitivity of culture compared to sequence-confirmed ProGastro SSCS results ranged from 52.9% to 76.9%, with the specificities ranging from 99.9% to 100%. Overall, these results suggest that the ProGastro SSCS assay is highly sensitive and specific in a clinical setting.


Assuntos
Infecções por Enterobacteriaceae/diagnóstico , Enterobacteriaceae/isolamento & purificação , Gastroenterite/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase em Tempo Real/métodos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Enterobacteriaceae/genética , Infecções por Enterobacteriaceae/microbiologia , Fezes/microbiologia , Feminino , Gastroenterite/microbiologia , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Sensibilidade e Especificidade , Estados Unidos , Adulto Jovem
6.
Pediatr Infect Dis J ; 32(7): 796-8, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23838781

RESUMO

Bordetella parapertussis is widely recognized as a cause of a pertussis-like respiratory illness in children, but reports of invasive infection are rare. We review the literature and describe the clinical presentation and treatment of 2 children with B. parapertussis bacteremia, as well as the techniques used to isolate the organism.


Assuntos
Bacteriemia/microbiologia , Bacteriemia/patologia , Infecções por Bordetella/microbiologia , Infecções por Bordetella/patologia , Bordetella parapertussis/isolamento & purificação , Bacteriemia/diagnóstico , Bacteriemia/tratamento farmacológico , Técnicas Bacteriológicas/métodos , Infecções por Bordetella/diagnóstico , Infecções por Bordetella/tratamento farmacológico , Criança , Humanos , Masculino
7.
Ann Am Thorac Soc ; 10(3): 235-8, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23802820

RESUMO

RATIONALE: Purulent pericarditis secondary to community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) is a potentially lethal infection that has yet to be described in the pediatric population. Only four cases of purulent pericarditis secondary to CA-MRSA have been described in the English literature, all of whom were adults. OBJECTIVES: We report on the first two pediatric cases of purulent pericarditis secondary to CA-MRSA to increase awareness of this potentially fatal condition. METHODS: Clinical data were obtained from an 8-year-old male patient and a 7-month-old female patient, both previously healthy, who presented to our hospital for treatment of severe shock and multiorgan failure. Literature review was performed using MEDLINE and Cochrane databases. Pulsed-field gel electrophoresis was performed to confirm the organism type. MEASUREMENTS AND MAIN RESULTS: Our previously healthy patients presented with refractory shock and were found to have purulent pericarditis with tamponade secondary to CA-MRSA. Both patients required emergent pericardiocentesis and surgical pericardial debridement. Isolates from both patients were found to be MRSA USA type 300, a common type of CA-MRSA that has become the most frequent cause of skin and soft tissue infections in the United States. CONCLUSIONS: Purulent pericarditis survival hinges upon early empiric antibiotic therapy targeting resistant Staphylococcus, rapid diagnostic efforts, and expeditious pericardial drainage when diagnosed. An aggressive multidisciplinary approach provided for complete recovery in both cases, and both children were discharged with normal cardiac function. These two cases emphasize the need for consideration of CA-MRSA presenting with purulent pericarditis as an etiology for refractory shock.


Assuntos
Tamponamento Cardíaco/diagnóstico , Diagnóstico Precoce , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Pericardite/diagnóstico , Infecções Estafilocócicas/diagnóstico , Tamponamento Cardíaco/etiologia , Criança , Diagnóstico Diferencial , Feminino , Humanos , Lactente , Masculino , Pericardite/complicações , Pericardite/microbiologia , Infecções Estafilocócicas/complicações , Infecções Estafilocócicas/microbiologia , Fatores de Tempo
8.
J Child Sex Abus ; 22(3): 297-311, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23590351

RESUMO

The Centers for Disease Control and Prevention recommends nucleic acid amplification testing for chlamydia and gonorrhea in sexually abused girls. No studies describe performance of APTIMA Combo 2 Assay with second target confirmation on the same testing platform. This nucleic acid amplification testing is evaluated within a large child advocacy center. Girls 3 to 18 years, 35% of whom reported consensual sexual activity, were prospectively tested by APTIMA Combo 2 on urine/vaginal swabs and by vaginal culture. A case of infection was defined as positive culture or positive urine or vaginal swab nucleic acid amplification testing with second target confirmation. Sensitivity of APTIMA Combo 2 on urine was found to be superior to vaginal culture and comparable to APTIMA Combo 2 on vaginal swabs for both infections. APTIMA Combo 2 on urine is less invasive, and its use may be preferred in this traumatized population.


Assuntos
Abuso Sexual na Infância/diagnóstico , Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/patogenicidade , Gonorreia/diagnóstico , Neisseria gonorrhoeae/patogenicidade , Técnicas de Amplificação de Ácido Nucleico/normas , Kit de Reagentes para Diagnóstico/normas , Vagina/microbiologia , Esfregaço Vaginal/normas , Adolescente , Centers for Disease Control and Prevention, U.S. , Criança , Defesa da Criança e do Adolescente/normas , Pré-Escolar , Infecções por Chlamydia/microbiologia , Infecções por Chlamydia/urina , Feminino , Gonorreia/microbiologia , Gonorreia/urina , Humanos , Estados Unidos
9.
Clin Infect Dis ; 56(3): 322-31, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23087388

RESUMO

BACKGROUND: During 9 May 2010-7 May 2011, an outbreak of pertussis-like illness (incidence, 80 cases per 100 000 persons) occurred in Franklin County, Ohio. The majority of cases were identified by IS481-directed polymerase chain reaction (PCR), which does not differentiate among Bordetella species. We sought to determine outbreak etiology and epidemiologic characteristics. METHODS: We obtained demographic, clinical, and vaccination-related data from the Ohio Disease Reporting System and Impact Statewide Immunization Information System. We tested sera from 14 patients for anti-pertussis toxin (PT) antibodies and used species-specific PCR on 298 nasopharyngeal specimens. RESULTS: Reported cases totaled 918. IS481 results were available for 10 serologically tested patients; 5 of 10 had discordant anti-PT antibody and IS481 results, suggestive of Bordetella holmesii, which lacks PT and harbors IS481. We identified specific Bordetella species in 164 of 298 specimens tested with multitarget PCR; B. holmesii and Bordetella pertussis were exclusively detected among 48 (29%) and 112 (68%), respectively; both were detected in 4 (2%). Among 48 patients with B. holmesii infections, 63% were aged 11-18 years, compared with 35% of 112 patients with B. pertussis infections (P = .001). Symptoms were similar among B. holmesii- and B. pertussis-infected patients. Adolescent pertussis ("Tdap") booster vaccinations were more effective against B. pertussis than B. holmesii (effectiveness: 67% and 36%, respectively; 95% confidence intervals, 38%-82% and -33% to 69%, respectively). CONCLUSIONS: We report the first documented mixed outbreak of B. pertussis and B. holmesii infections. Bordetella holmesii particularly affected adolescents. Although laboratory capacity limitations might inhibit routine use of multitarget PCR for clinical diagnosis, focused testing and enhanced surveillance might improve understanding the burden of B. holmesii infection.


Assuntos
Bordetella pertussis/isolamento & purificação , Bordetella/isolamento & purificação , Surtos de Doenças , Coqueluche/epidemiologia , Adolescente , Anticorpos Antibacterianos/sangue , Técnicas de Tipagem Bacteriana , Bordetella/classificação , Bordetella/imunologia , Bordetella pertussis/classificação , Bordetella pertussis/imunologia , Criança , DNA Bacteriano/análise , Humanos , Nasofaringe/microbiologia , Ohio/epidemiologia , Toxina Pertussis/imunologia , Reação em Cadeia da Polimerase/métodos , Coqueluche/microbiologia
10.
J Pediatr ; 161(2): 357-61, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22608699

RESUMO

The use of herpes simplex virus (HSV) polymerase chain reaction for diagnosis of HSV disease involving the central nervous system has not translated into widespread use for the detection of DNAemia. We report our 6-year experience using blood polymerase chain reaction testing for HSV infection in neonates and older children with HSV disease.


Assuntos
DNA Viral/sangue , Herpes Simples/diagnóstico , Reação em Cadeia da Polimerase , Simplexvirus/isolamento & purificação , Adolescente , Viroses do Sistema Nervoso Central/diagnóstico , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino
12.
J AAPOS ; 15(3): 230-3, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21665501

RESUMO

PURPOSE: To investigate the effectiveness of 3 surgical preparation techniques in decreasing bacterial contamination of needles and suture material during strabismus surgery. METHODS: Consecutive patients requiring 2-muscle strabismus surgery were randomized into 1 of 3 groups. In Group A, patients' periocular skin and bulbar conjunctivae underwent preparation with 5% povidone-iodine; the drape was placed without regard to eyebrows; and an open wire-loop lid speculum was used. Group B patients underwent the same preparation as Group A patients; however, the eyelashes and eyebrows were scrubbed with 5% povidone-iodine on cotton tip applicators, and the drape was placed to exclude the eyebrows from the surgical field. Group C patients underwent the same preparation as Group B patients; however, a bladed lid speculum was used during surgery to exclude some of the eyelashes from the surgical field. After the procedure, all needles and suture materials were sent separately for aerobic culture. The data were analyzed for differences in contamination rates between the groups. RESULTS: Of 77 patients, 24 (31.4%) had either a needle and/or suture contaminant. Groups A, B, and C had mean contamination rates of 29.6%, 34.6%, and 29.2%, respectively. There was no significant statistical variation in contamination among the 3 groups. The most common organism identified was a coagulase-negative staphylococcus strain. CONCLUSIONS: More meticulous sterile preparation of the surgical field did not result in a meaningful reduction in suture or needle contamination rates during strabismus surgery.


Assuntos
Carga Bacteriana , Contaminação de Equipamentos/prevenção & controle , Agulhas/microbiologia , Procedimentos Cirúrgicos Oftalmológicos/métodos , Estrabismo/cirurgia , Suturas/microbiologia , Anti-Infecciosos Locais/administração & dosagem , Bactérias/isolamento & purificação , Endoftalmite/prevenção & controle , Sobrancelhas/efeitos dos fármacos , Sobrancelhas/microbiologia , Pestanas/efeitos dos fármacos , Pestanas/microbiologia , Humanos , Músculos Oculomotores/cirurgia , Complicações Pós-Operatórias/prevenção & controle , Povidona-Iodo/administração & dosagem
13.
J Clin Microbiol ; 49(7): 2390-4, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21543568

RESUMO

In October 2009, the Centers for Disease Control and Prevention recommended that clinical laboratories test all stools submitted for the detection of enteric bacterial pathogens for the presence of Shiga toxin-producing Escherichia coli (STEC). In order to do this, it is recommended that all stools be cultured for Escherichia coli O157:H7 on selective medium as well as that testing for the presence of Shiga toxin be done by immunoassay to detect non-O157 STEC (3). There are a variety of products that are FDA approved for detection of Shiga toxin. Further, it is recommended that Shiga toxin detection be done by testing overnight enrichment broth cultures of stools rather than directly examining stools for this toxin. This recommendation was made approximately 18 months ago. We have asked Mario Marcon of Nationwide's Children Hospital in Columbus, OH, to explain the rationale for his decision to follow this recommendation, while we have asked Deanna Kiska and Scott Riddell of Upstate University Hospital in Syracuse, NY, why these guidelines have not been adopted by their laboratory.


Assuntos
Técnicas Bacteriológicas/métodos , Infecções por Escherichia coli/diagnóstico , Infecções por Escherichia coli/epidemiologia , Fezes/microbiologia , Guias como Assunto , Escherichia coli Shiga Toxigênica/isolamento & purificação , Técnicas Bacteriológicas/economia , Centers for Disease Control and Prevention, U.S. , Meios de Cultura/química , Meios de Cultura/economia , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/mortalidade , Fidelidade a Diretrizes , Humanos , Imunoensaio/economia , Imunoensaio/métodos , Incidência , Prevalência , Toxina Shiga/análise , Estados Unidos/epidemiologia
14.
J Antimicrob Chemother ; 66(3): 499-504, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21212056

RESUMO

OBJECTIVES: The aim of this study was to elucidate the role of two novel membrane fusion proteins (MFPs) in the susceptibility of Acinetobacter baumannii to antimicrobial agents. METHODS: The genome sequence of A. baumannii ATCC 17978 contains two open reading frames (ORFs) annotated as AdeT in the NCBI genome database. Both the putative efflux genes display >30% similarity to known MFPs. The antimicrobial susceptibility profiles of Escherichia coli KAM32 cells carrying the genes were monitored by the broth dilution method. Different efflux pump inhibitors were used for fluorimetric efflux assays. The functions of putative ORFs were confirmed in A. baumannii by insertional inactivation and complementation. RESULTS: E. coli cells carrying the ORFs had decreased susceptibility to antibiotics, disinfectants, dyes and detergents, with enhanced efflux activity. Inactivation of the ORFs and further characterization in A. baumannii confirmed its role in antimicrobial resistance by active efflux. CONCLUSIONS: This report describes the functions of novel resistance determinants, members of the MFP family, for the first time in A. baumannii.


Assuntos
Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Farmacorresistência Bacteriana , Proteínas de Fusão de Membrana/genética , Proteínas de Fusão de Membrana/metabolismo , Antibacterianos/farmacologia , Clonagem Molecular , DNA Bacteriano/química , DNA Bacteriano/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Humanos , Dados de Sequência Molecular , Mutagênese Insercional , Fases de Leitura Aberta , Análise de Sequência de DNA
15.
J Clin Microbiol ; 49(2): 534-8, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21123535

RESUMO

To determine the prevalence of serotypes of Streptococcus pneumoniae responsible for pneumonia with pleural effusion, we determined the capsular polysaccharide (PS) type directly on 49 pleural fluid specimens collected from pediatric patients during 2007 to 2009 with laboratory-confirmed pneumococcal pneumonia by using monoclonal antibodies and a multiplex, bead array immunoassay. Because the fluids had to be heated to remove nonspecific reactivity before being tested in the immunoassay and type 19A PS is heat labile, the pleural fluid samples were also tested for serotype 19A capsule gene locus by PCR. Use of the multiplex immunoassay combined with type-specific 19A PCR allowed for serotype determination on 40 of 49 pleural fluids. Pneumococcal pneumonia with pleural effusion was associated with a limited number of serotypes, with types 1, 3, 7F/A, and 19A accounting for 75% of the typeable cases. The concentration of capsular PS in the pleural fluids was often greater than 1 µg/ml and sufficient to inhibit the opsonic capacity of sera from individuals who had received the 23-valent pneumococcal PS vaccine. Based on the serotypes observed before and after introduction of the 7-valent pneumococcal conjugate vaccine, the recently licensed 13-valent pneumococcal conjugate vaccine may reduce the incidence of pneumonia with pleural effusions.


Assuntos
Técnicas de Tipagem Bacteriana/métodos , Derrame Pleural/patologia , Pneumonia Pneumocócica/complicações , Pneumonia Pneumocócica/microbiologia , Streptococcus pneumoniae/classificação , Streptococcus pneumoniae/isolamento & purificação , Adolescente , Anticorpos Antibacterianos , Anticorpos Monoclonais , Antígenos de Bactérias/imunologia , Cápsulas Bacterianas/imunologia , Criança , Pré-Escolar , Humanos , Imunoensaio/métodos , Lactente , Derrame Pleural/diagnóstico , Vacinas Pneumocócicas/imunologia , Sorotipagem/métodos
16.
J Clin Microbiol ; 48(7): 2337-43, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20463154

RESUMO

This multicenter study evaluated the clinical performance of the 3M Rapid Detection RSV test (3MRSV) compared to a composite reference standard of R-Mix culture and direct specimen immunofluorescence for detection of respiratory syncytial virus (RSV). The performance of the BinaxNOW RSV test was also evaluated using this reference standard. In a secondary analysis, discordant results were arbitrated using the Gen-Probe/Prodesse ProFlu+ reverse transcription-PCR (RT-PCR) assay. Subjects were stratified into three groups as follows: group 1 (G1), all ages; G2, subjects <22 years old (FDA-cleared ages for 3MRSV testing); and G3, subjects <5 years old (FDA-cleared ages for BinaxNOW RSV testing). A total of 1,306 specimens (G1, n = 1,306; G2, n = 1,140; G3, n = 953) from subjects of all ages presenting with respiratory symptoms met study criteria for analysis. Sensitivities, specificities, positive predictive values, and negative predictive values of 3MRSV for G1 were 86.5%, 95.8%, 91.4%, and 93.2%, respectively, and those for G2 were 87.3%, 95.6%, 92.4%, and 92.5%, respectively. For those samples analyzed by both 3MRSV and BinaxNOW, the 3MRSV was more sensitive (G1, 86.3%; G2, 87.2%; and G3, 89.9%) than was BinaxNOW (G1, 70.84%; G2, 72.0%; and G3, 72.4%) (P < 0.05). Specificities for RSV detection from nasopharyngeal (NP) aspirates and NP swabs for all groups were comparable for 3MRSV and BinaxNOW, but 3MRSV was less specific than BinaxNOW when nasal washes/aspirates were tested (P < 0.05). The 3MRSV assay performed well for the detection of RSV, and the overall assay performance was superior to that of BinaxNOW. The 3MRSV reader eliminated user misinterpretation and provided test result and quality control documentation.


Assuntos
Antígenos Virais/análise , Imunoensaio/métodos , Infecções por Vírus Respiratório Sincicial/diagnóstico , Vírus Sinciciais Respiratórios/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Secreções Corporais/virologia , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Pulmão/virologia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Kit de Reagentes para Diagnóstico/virologia , Estatísticas não Paramétricas , Fatores de Tempo
17.
J Clin Microbiol ; 48(3): 852-6, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20053857

RESUMO

Rapid antigen testing of upper respiratory secretions collected with various swab types is often utilized for laboratory diagnoses of influenza virus infection. There are limited data on the effects of swab composition on test performance. This study compared the performance of the Quidel QuickVue Influenza A+B test on secretions from the anterior nares when a polyurethane foam swab was used for collection to that when a nylon flocked swab was used for collection. One hundred subjects who presented to a pediatric emergency department with symptoms suggestive of an influenza virus infection were recruited for the study. Foam and flocked swabs of the anterior nares were obtained from separate nares of each subject before a posterior nasopharyngeal swab was collected and placed into viral transport medium. The QuickVue test was performed directly on each swab type, and the results were compared to the results of reverse transcription-PCR (RT-PCR), direct fluorescent antibody (DFA) test, and viral culture performed on the transport medium. RT-PCR alone and DFA combined with culture were utilized as separate gold standards. There were 56 cases of influenza detected by RT-PCR; the QuickVue test was positive for 40 foam and 30 flocked swabs, for sensitivities of 71% and 54%, respectively (P = 0.01). Similarly, there were 49 influenza cases detected by DFA and/or culture; the QuickVue test was positive for 38 foam and 30 flocked swabs, for sensitivities of 78% and 61%, respectively (P = 0.13). This study suggests that polyurethane foam swabs perform better than nylon flocked swabs for the collection of secretions from anterior nares in the Quidel QuickVue Influenza A+B test.


Assuntos
Antígenos Virais/análise , Secreções Corporais/virologia , Técnicas de Laboratório Clínico/métodos , Influenza Humana/diagnóstico , Nariz/virologia , Orthomyxoviridae/isolamento & purificação , Manejo de Espécimes/métodos , Adolescente , Criança , Serviços Médicos de Emergência , Humanos , Nasofaringe/virologia , Nylons , Poliuretanos , Sensibilidade e Especificidade
18.
J Mol Diagn ; 12(1): 102-8, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19948820

RESUMO

One-step, real-time PCR assays for rhinovirus have been developed for a limited number of PCR amplification platforms and chemistries, and some exhibit cross-reactivity with genetically similar enteroviruses. We developed a one-step, real-time PCR assay for rhinovirus by using a sequence detection system (Applied Biosystems; Foster City, CA). The primers were designed to amplify a 120-base target in the noncoding region of picornavirus RNA, and a TaqMan (Applied Biosystems) degenerate probe was designed for the specific detection of rhinovirus amplicons. The PCR assay had no cross-reactivity with a panel of 76 nontarget nucleic acids, which included RNAs from 43 enterovirus strains. Excellent lower limits of detection relative to viral culture were observed for the PCR assay by using 38 of 40 rhinovirus reference strains representing different serotypes, which could reproducibly detect rhinovirus serotype 2 in viral transport medium containing 10 to 10,000 TCID(50) (50% tissue culture infectious dose endpoint) units/ml of the virus. However, for rhinovirus serotypes 59 and 69, the PCR assay was less sensitive than culture. Testing of 48 clinical specimens from children with cold-like illnesses for rhinovirus by the PCR and culture assays yielded detection rates of 16.7% and 6.3%, respectively. For a batch of 10 specimens, the entire assay was completed in 4.5 hours. This real-time PCR assay enables detection of many rhinovirus serotypes with the Applied Biosystems reagent-instrument platform.


Assuntos
Infecções por Picornaviridae/diagnóstico , Reação em Cadeia da Polimerase/métodos , Rhinovirus/isolamento & purificação , Criança , Biologia Computacional , Primers do DNA/genética , Humanos , Limite de Detecção , Reação em Cadeia da Polimerase/economia , RNA Viral/genética , RNA Viral/isolamento & purificação , Reprodutibilidade dos Testes , Rhinovirus/genética , Fatores de Tempo
19.
Ann Clin Microbiol Antimicrob ; 8: 21, 2009 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-19531268

RESUMO

BACKGROUND: Over the last decade, nosocomial infections due to Acinetobacter baumannii have been described with an increasing trend towards multidrug resistance, mostly in intensive care units. The aim of the present study was to determine the clonal relatedness of clinical isolates and to elucidate the genetic basis of imipenem resistance. METHODS: A. baumannii isolates (n = 83) originated from two hospital settings in central Ohio were used in this study. Pulsed-field gel electrophoresis genotyping and antimicrobial susceptibility testing for clinically relevant antimicrobials were performed. Resistance determinants were characterized by using different phenotypic (accumulation assay for efflux) and genotypic (PCR, DNA sequencing, plasmid analysis and electroporation) approaches. RESULTS: The isolates were predominantly multidrug resistant (>79.5%) and comprised of thirteen unique pulsotypes, with genotype VII circulating in both hospitals. The presence of bla(OXA-23) in 13% (11/83) and IS(Aba1) linked bla(OXA-66) in 79.5% (66/83) of clinical isolates was associated with high level imipenem resistance. In this set of OXA producing isolates, multidrug resistance was bestowed by bla(ADC-25), class 1 integron-borne aminoglycoside modifying enzymes, presence of sense mutations in gyrA/parC and involvement of active efflux (with evidence for the presence of adeB efflux gene). CONCLUSION: This study underscores the major role of carbapenem-hydrolyzing class D beta-lactamases, and in particular the acquired OXA-23, in the dissemination of imipenem-resistant A. baumannii. The co-occurrence of additional resistance determinant could also be a significant threat.


Assuntos
Infecções por Acinetobacter/epidemiologia , Infecções por Acinetobacter/microbiologia , Acinetobacter baumannii/classificação , Acinetobacter baumannii/genética , Farmacorresistência Bacteriana Múltipla , Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Proteínas de Bactérias/genética , Técnicas de Tipagem Bacteriana/métodos , Análise por Conglomerados , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Impressões Digitais de DNA , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Humanos , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Epidemiologia Molecular , Ohio/epidemiologia , Adulto Jovem , beta-Lactamases/genética
20.
Acad Emerg Med ; 15(5): 437-44, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18439199

RESUMO

OBJECTIVES: Among children with cerebrospinal fluid (CSF) pleocytosis, the task of separating aseptic from bacterial meningitis is hampered when the CSF Gram stain result is unavailable, delayed, or negative. In this study, the authors derive and validate a clinical decision rule for use in this setting. METHODS: This was a review of peripheral blood and CSF test results from 78 children (< 19 years) presenting to Children's Hospital Columbus from 1998 to 2002. For those with a CSF leukocyte count of > 7/microL, a rule was created for separating bacterial from viral meningitis that was based on routine laboratory tests, but excluded Gram stain. The rule was validated in 158 subjects seen at the same site (Columbus, 2002-2004) and in 871 subjects selected from a separate site (Boston, 1993-1999). RESULTS: One point each (maximum, 6 points) was assigned for leukocytes > 597/microL, neutrophils > 74%, glucose < 38 mg/dL, and protein > 97 mg/dL in CSF and for leukocytes > 17,000/mL and bands to neutrophils > 11% in peripheral blood. Areas under receiver-operator-characteristic curves (AROCs) for the resultant score were 0.98 for the derivation set and 0.90 and 0.97, respectively, for validation sets from Columbus and Boston. Sensitivity and specificity pairs for the Boston data set were 100 and 44%, respectively, at a score of 0 and 97 and 81% at a score of 1. Likelihood ratios (LRs) increased from 0 at a score of 0 to 40 at a score of > or = 4. CONCLUSIONS: Among children with CSF pleocytosis, a prediction score based on common tests of CSF and peripheral blood and intended for children with unavailable, negative, or delayed CSF Gram stain results has value for diagnosing bacterial meningitis.


Assuntos
Técnicas de Apoio para a Decisão , Leucocitose/líquido cefalorraquidiano , Adolescente , Boston , Criança , Pré-Escolar , Hospitais Pediátricos , Humanos , Lactente , Recém-Nascido , Meningites Bacterianas/líquido cefalorraquidiano , Meningites Bacterianas/classificação , Meningites Bacterianas/microbiologia , Estudos Multicêntricos como Assunto , Ohio , Valor Preditivo dos Testes , Curva ROC , Estudos Retrospectivos
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