RESUMO
BACKGROUND: The aim of the study was to determine the incidence of parasitic liver cysts. MATERIAL AND METHODS: In 1996-2000 at the Department of Infectious Diseases parasitic liver cysts were diagnosed in 31 patients. The diagnosis was based on imaging examinations (ultrasound, computerised tomography), serological reactions (ELISA, IHA) and histopathological investigation of the specimens obtained through fine-needle aspiration biopsy. RESULTS AND CONCLUSION: The latter followed the pre-treatment with antiparasitic drug and no significant complications were observed. On the basis of the criteria developed by our team (evident, highly probable and probable diagnosis), hydatid disease of the liver was diagnosed in 8 patients (25.8%). The remaining subjects, excluding one patient who underwent surgical treatment, received repeated treatment with imidazole derivatives (Zentel or Vermox).
Assuntos
Equinococose Hepática/diagnóstico , Biópsia por Agulha , Pré-Escolar , Diagnóstico Diferencial , Equinococose Hepática/patologia , Ensaio de Imunoadsorção Enzimática , Feminino , Testes de Hemaglutinação , Humanos , Lactente , Masculino , Estudos ProspectivosRESUMO
We report here an unusual sporadic case of Creutzfeldt-Jakob disease (CJD) characterized by an abundance of prion protein (PrP)-immunopositive kuru and multicentric but not florid plaques. Molecular genetic analysis of the PRNP open reading frame region spanning codons 8-221 was performed. Neither deletion nor insertion mutations were detected in the repeat area of the PRNP. No pathogenic mutation was found in the sequenced region between codon 108-221. Restriction analysis of the amplified fragment using restriction endonucleases DdeI, PvuII and AluI did not show any of the previously described pathogenic mutations at codon 102, 105, and 117 associated with Gerstmann-Sträussler-Scheinker (GSS). The patient was heterozygous for the methionine/valine coding triplet at polymorphic codon 129 of the PRNP gene by sequence, restriction endonuclease analysis and hybridization with allele-specific nucleotides. Furthermore, hybridization with 32P-labeled allele-specific oligonucleotides confirmed the absence of pathogenic mutations at codons 102, 200 and 178. Such a case may present a missing "link" between sporadic CJD and familial GSS.
Assuntos
Amiloide/genética , Síndrome de Creutzfeldt-Jakob/genética , Doença de Gerstmann-Straussler-Scheinker/genética , Precursores de Proteínas/genética , Síndrome de Creutzfeldt-Jakob/patologia , Heterozigoto , Humanos , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Biologia Molecular/métodos , Fases de Leitura Aberta/genética , Fenótipo , Proteínas Priônicas , PríonsRESUMO
A case of angioimmunoblastic lymphadenopathy in a boy aged 19 years was observed, with long-standing fever, peripheral lymphadenopathy, considerable weight loss. In the differential diagnosis septicaemia was considered, including tuberculous septicaemia, collagen diseases, neoplasms and lymphoproliferative diseases. Attention is called to the similarity of this symptom complex to that observed in AIDS.
