Assuntos
Líquido da Lavagem Broncoalveolar/citologia , Compostos Organometálicos/toxicidade , Fosfatase Ácida/metabolismo , Fosfatase Alcalina/metabolismo , Animais , Permeabilidade da Membrana Celular/efeitos dos fármacos , Glucose/metabolismo , Injeções Espinhais , L-Lactato Desidrogenase/metabolismo , Pulmão/efeitos dos fármacos , Pulmão/enzimologia , Pulmão/patologia , Masculino , Tamanho do Órgão/efeitos dos fármacos , Compostos Organometálicos/administração & dosagem , Ratos , Ratos Wistar , Fatores de TempoRESUMO
Experiments involving 120 male Wistar rats were performed to study the effect of treatment with cadmium acetate and external irradiation. A single 0.5 mg/kg body weight dose of cadmium acetate was administered intratracheally. Shortly thereafter, the animals received a single whole-body exposure to 4 Gy gamma rays (cesium source). Findings indicated the chemical elevated enzyme activities of lactate dehydrogenase (LDH), alkaline phosphatase (AlP), and acid phosphatase (AP), as well as protein content and percentage of neutrophils in bronchoalveolar lavage fluid (BALF); the percentage of alveolar macrophages was sharply reduced. Radiation alone produced no substantial changes in the parameters investigated. Treatment with both agents combined was found to result in a synergistic rise of LDH, AlP, and AP activities and protein content in BALF. It was concluded that the BALF biochemical markers used are reliable indicators for identifying the type of combined effect produced in the lungs by chemical agents and ionizing radiation.
Assuntos
Acetatos , Líquido da Lavagem Broncoalveolar/química , Cádmio/toxicidade , Pulmão/efeitos dos fármacos , Pulmão/efeitos da radiação , Linfócitos/efeitos dos fármacos , Lesões Experimentais por Radiação/patologia , Animais , Sinergismo Farmacológico , Pulmão/patologia , Linfócitos/efeitos da radiação , Masculino , Tamanho do Órgão/efeitos dos fármacos , Tamanho do Órgão/efeitos da radiação , Ratos , Ratos Wistar , Irradiação Corporal TotalRESUMO
One-hundred male white rats were given a single intratracheal dose of 0, 5 mg kg-1 cadmium acetate. There was a fall in catalase (CAT) and superoxide dismutase (SOD) in lung homogenate throughout the 30 d after treatment. Non-protein sulphhydryl (NPSH) content, glucose-6-phosphate dehydrogenase (G-6-PD) and glutathione peroxidase (GP) were all increased from days 5 to 15. There was an increase in lactate dehydrogenase (LDH) and protein in bronchoalveolar lavage fluid (BALF) and in the relative weight of the lungs which provide evidence of severe toxic lesions of the lungs. Increased lipid peroxidation provoked by the reduced lung antioxidant protective capacity may be an important mechanism in the pulmonary damage caused by cadmium.
Assuntos
Acetatos , Cádmio/toxicidade , Pulmão/efeitos dos fármacos , Animais , Líquido da Lavagem Broncoalveolar/enzimologia , Catalase/metabolismo , Glucosefosfato Desidrogenase/metabolismo , Glutationa Peroxidase/metabolismo , Pulmão/enzimologia , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ratos , Ratos Endogâmicos , Superóxido Dismutase/metabolismo , TraqueiaRESUMO
A histological study was made on experimentally damaged ligament apparatus of the ankle joint of rabbits, treated with celestone, divided into three groups: celestone was administered immediately after the damage in the first group. Morphological changes were manifested slightly. Celestone was administered on the 20th day after the damage in the second group. A manifested chronic productive inflammatory process with hyalinization was established morphologically. The most manifested changes were found in the control group of rabbits, which were not treated with celestone. Intraarticular, administration of celestone in ligament damages immediately after trauma prevents occurrence of severe fibrous changes and contributes for functional recovery of the ligament apparatus.
