[Evaluation of the efficiency of a new dried nutrient medium for isolating Corynebacterium diphtheriae]. / Otsenka éffektivnosti novoi sukhoi pitatel'noi sredy dlia vydelenii Corynebacterium diphtheriae.

Tellurite blood media prepared on a new dried base with amino peptide have been found to have advantages, when used for the bacteriological diagnosis of C. diphtheriae infection, over Buchin's selective quinosol medium with respect to the rate and time of the isolation of C. diphtheriae; besides, these new media have been found to possess pronounced differentiating properties with respect to the concomitant microflora.

[A new dried nutrient medium for the isolation of Corynebacterium diphtheriae (its development and experimental study)]. / Novaia sukhaia pitatel'naia sreda dlia vydeleniia Corynebacterium diphtheriae (razrabotka i éksperimental'noe izuchenie).

A new dried diagnostic medium for the isolation of C. diphtheriae has been developed on the basis of aminopeptide. This new aminopeptide-based medium compares favorably with Buchin's medium in its growth and inhibitory properties.

[Study of the enterotoxic action of the neurotoxin of the Sonne dysentery microbe in a rabbit small intestine loop model]. / Izuchenie énterotoksicheskogo deistviia neirotoksina dizenteriinogo mikroba Zonne na modeli izolirovannoi petli tonkogo kishechnika krolika.

The enterotoxic action of neurotoxin from Sonne dysentery microbes (obtained by the method of Mesrobeanu et al.), and also of the culture autolysates and homologous Boiven's endotoxin was studied on a model of the isolated loop of the rabbit small intestine. Neurotoxin preparations obtained from virulent strains as well as autolysates of these cultures possessed enterotoxic activity, whereas purifed endotoxin preparations in doses of 1--10 mg failed to cause any dilatation of the isolated intestinal segment. A significant individual rabbit sensitivity to the enterotoxic action of the neurotoxin preparation was revealed. Lyophilization of neurotoxin preparation did not influence its enterotoxicity. However dialysis against distilled water and boiling of the neurotoxin preparations led to the loss of enterotoxic activity.

[Enterotoxic activity of live cultures of Sonne dysentery microbes and formation of enterotoxin in vivo]. / Enterotoksicheskaia aktivnost' zhivykh kul'tur dizenteriinogo mikroba Zonne i obrazovanie énterotoksina in vivo.

The great majority of virulent Sonne dysentery bacillus cultures caused an accumulation of the fluid in the lumen of the isolated segment of the rabbit small intestine containing a great amount of mucus and sometimes of the blood as well; the segment mucosa was hyperemic and had punctate hemorrhages. Avirulent microbial cultures as a rule caused no exudation in the intestinal lumen. Sterile and concentrated contents of the intestinal loops responding to the centrated contents of the intestinal loops responding to the administration of the virulent culture of Sonne bacillus or a toxigenic strain of Grigoriev-Shiga dysentery bacillus caused a positive response in other rabbits. The character of the exudate and the changes in the mucosa failed to differ from such following the administration of live cultures.

[Dynamics of the establishment of intestinal microflora in healthy full-term newborns]. / Dinamika stanovleniia mikroflory kishechnika zdorovykh donoshennykh novorozhdennykh detei

A study was made of the dynamics of establishment of the intestinal microflora in 79 healthy mature neonates born of healthy mothers. A total of 223 bacteriological examinations were conducted. A stable prevalence of bifidobacteria over the aerobic portion of the intestinal biocenosis occurred in all the children from the 8th day after birth. Along with this the following bacteria were continuously present in the intestine: lactic bacilli and streptococci E. coli, enterococci and staphylococci; however, their portion in the biocenosis was not great. Bacteroids, microbes of the Proteus genus, and yeast-like fungi were found in the cultures only before the establishment of a high bifidobacteria level. Conditionally pathogenic microorganisms and their associations were continuously revealed in 19.8% of healthy mature neonates. Apparently the qualitative prevalence of bifidobacteria prevented the clinical expression of dysbacteriosis in these children.