Impact of PEG Content on Doxorubicin Release from PLGA-co-PEG Nanoparticles.

Nanoparticles (NPs) have become attractive vehicles for drug delivery in cancer therapy due to their ability to accumulate in tumours and mitigate side effects. This study focuses on the production of doxorubicin (DOX)-loaded NPs comprising Poly (lactic-co-glycolic acid)-Polyethylene glycol with varying PEG proportions and the examination of their impact on drug release kinetics. DOX-loaded NPs, composed of PLGA-co-PEG with PEG contents of 0%, 5%, 10%, and 15%, were synthesized by the solvent evaporation technique, exhibited spherical morphology, and had sizes ranging from 420 nm to 690 nm. In vitro drug release studies revealed biphasic profiles, with higher PEG contents leading to faster and more extensive drug release. The Baker-Lonsdale model demonstrated the best fit to the drug release data, indicating that the release process is diffusion-controlled. The diffusion coefficients for DOX determined ranged from 6.3 × 10-18 to 7.55 × 10-17 cm2s-1 and exhibited an upward trend with increasing PEG content in the polymer. In vitro cytotoxicity tests with CHO cells showed that unloaded NPs are non-toxic, while DOX-loaded PLGA-PEG 15% NPs induced a greater decrease in cellular viability compared to their PLGA counterparts. A mathematical relationship between the diffusion coefficient and PEG percentage was derived, providing a practical tool for optimizing DOX release profiles.

Nomophobia and Phubbing Levels of Nursing Students: A Multicenter Study.

Today, with the enhancement in the usage of smartphones, the concepts of nomophobia and phubbing have emerged. Nomophobia refers to the fear of being deprived of smartphones/smart devices. Phubbing is the use of a person's smartphone in situations that are not appropriate for the situation, time, and place. Therefore, the study purposed to evaluate nursing students' nomophobia and phubbing scores in Turkey, Portugal, and the United States. The data were collected with the Personal Information Questionnaire, Nomophobia Scale, and Phubbing Scale from N = 446 nursing students. The mean age of the students was 22.04 ± 4.08 years, and 86.5% were women. It was found that the total nomophobia scores of the nursing students were 80.15 ± 21.96, 72.29 ± 28.09, and 99.65 ± 6.11, respectively in Turkey, Portugal, and the United States. When the countries' Nomophobia Scale total scores, "giving up convenience," "not being able to communicate," and "losing connectedness" scores were compared with each other, they were found to be statistically significant (P < .05). When the countries' Phubbing Scale total scores and all subscale scores were compared with each other were found to be statistically significant (P < .05). It is seen that nomophobia scores were moderate (60 ≤ NMP-Q nomophobia ≤ 99) and phubbing scores (<40) were below the level indicating addiction in all countries.

Trueness of Extraoral Digital Impressions for Full-Arch Implant Impressions-In Vitro Study.

Direct scanning of silicone impressions is a valid technique. However, studies in implant-supported rehabilitations are lacking. This in vitro study aims to compare the trueness of impressions obtained with two types of silicone and their corresponding stone casts, using two laboratory scanners in a full-arch implant rehabilitation. A master cast with six dental implants was scanned with a 12-megapixel scanner to obtain a digital master cast. Ten implant impressions were made using two silicones (Zhermack and Coltene) with the open-tray technique. The impressions and stone casts were scanned by two extraoral scanners (Identica T500, Medit; and S600 ARTI, Zirkonzhan). Trueness was assessed by comparing linear and angular distances in digital casts with the master cast. A p < 0.05 significance level was considered. The results showed that for the linear measurements, 72% were higher than the master cast measurements, and no consistent pattern was observed in the angular measurements. The greatest deviations were detected between the most posterior implants, with mean values ranging between 173 and 314 µm. No significant differences were found between scanners. However, differences were observed in the distances between silicones (46.7%) and between impressions and stone casts (73.3%). This work demonstrates that the direct scanning of silicone impressions yields results comparable to those obtained from scanning gypsum casts in full-arch implant-supported rehabilitation.

In vitro selection of DNA aptamers against staphylococcal enterotoxin A.

Staphylococcal enterotoxin A (SEA) is the most frequently reported in staphylococcal food poisoning (SFP) outbreaks. Aptamers are single-stranded nucleic acids that are seen as promising alternatives to antibodies in several areas, including diagnostics. In this work, systematic evolution of ligands by exponential enrichment (SELEX) was used to select DNA aptamers against SEA. The SELEX protocol employed magnetic beads as an immobilization matrix for the target molecule and real-time quantitative PCR (qPCR) for monitoring and optimizing sequence enrichment. After 10 selection cycles, the ssDNA pool with the highest affinity was sequenced by next generation sequencing (NGS). Approximately 3 million aptamer candidates were identified, and the most representative cluster sequences were selected for further characterization. The aptamer with the highest affinity showed an experimental dissociation constant (KD) of 13.36 ± 18.62 nM. Increased temperature negatively affected the affinity of the aptamer for the target. Application of the selected aptamers in a lateral flow assay demonstrated their functionality in detecting samples containing 100 ng SEA, the minimum amount capable of causing food poisoning. Overall, the applicability of DNA aptamers in SEA recognition was demonstrated and characterized under different conditions, paving the way for the development of diagnostic tools.

Fatores preditores associados a ocorrência de lesão por pressão em pacientes em cuidados paliativos oncológicos / Predictive factors associated with the occurrence of pressure injuries in patients undergoing cancer palliative care / Factores predictivos asociados con la aparición de lesiones por presión en pacientes sometidos a cuidados paliativos oncológicos

Objetivo: Avaliar os fatores preditores para ocorrência de lesão por pressão em pacientes oncológicos em cuidados paliativos. Métodos: Estudo epidemiológico observacional, analítico, com delineamento transversal e abordagem quantitativa. A coleta de dados foi realizada com 105 participantes, no período de maio a outubro de 2019, em uma Clínica de Cuidados Paliativos Oncológicos de um Centro de Alta Complexidade em Oncologia. Os foram inseridos no software Biostat 5.0, em que foi realizada primeiramente a análise de regressão logística univariada, e posteriormente foram selecionadas as variáveis para a regressão logística múltipla e assim definiram-se os fatores preditivos para lesão por pressão. Resultados: A prevalência identificada foi de 19,04% para lesão por pressão. A maioria da amostra eram mulheres (60%), com idade menor que 70 anos (70%). Dois terços apresentavam risco muito alto (15%), para lesão por pressão segundo a Escala de Braden, e possuíam como diagnóstico primário câncer de próstata (20%), seguido de colo uterino (15%). Conclusão: A presença de lesão medular e o uso de fralda descartável demonstrou forte correlação com o desenvolvimento de lesão por pressão, sendo estes os fatores preditivos identificados neste estudo. Conhecer o perfil desses pacientes auxilia na elaboração e sistematização das condutas de enfermagem, visando melhor qualidade e segurança no cuidado. (AU)

Objective: To evaluate the predictive factors for the occurrence of pressure injuries in cancer patients undergoing palliative care. Methods: Observational, analytical, cross-sectional epidemiological study with a quantitative approach. Data collection was carried out with 105 participants, from May to October 2019, in an Oncology Palliative Care Clinic of a High Complexity Oncology Center. The data were entered into the Biostat 5.0 software, in which the univariate logistic regression analysis was first performed, and then the variables for the multiple logistic regression were selected, thus defining the predictive factors for pressure injury. Results: The identified prevalence was 19.04% for pressure injuries. Most of the sample were women (60%), aged under 70 years (70%). Two-thirds were at very high risk (15%) for pressure injury according to the Braden Scale, and had prostate cancer as a primary diagnosis (20%), followed by cervix (15%). Conclusion: The presence of spinal cord injury and the use of a disposable diaper showed a strong correlation with the development of pressure injury, which are the predictive factors identified in this study. Knowing the profile of these patients helps in the elaboration and systematization of nursing procedures, aiming at better quality and safety in care. (AU)

Objetivo: Evaluar los factores predictivos de la ocurrencia de lesiones por presión en pacientes oncológicos sometidos a cuidados paliativos. Métodos: Estudio epidemiológico observacional, analítico, transversal con enfoque cuantitativo. La recolección de datos se realizó con 105 participantes, de mayo a octubre de 2019, en una Clínica de Cuidados Paliativos Oncológicos de un Centro Oncológico de Alta Complejidad. Los datos se ingresaron en el software Biostat 5.0, en el cual se realizó primero el análisis de regresión logística univariante, y luego se seleccionaron las variables para la regresión logística múltiple, definiendo así los factores predictivos de lesión por presión. Resultados: La prevalencia identificada fue del 19,04% para las lesiones por presión. La mayoría de la muestra fueron mujeres (60%), menores de 70 años (70%). Dos tercios tenían un riesgo muy alto (15%) de lesión por presión según la escala de Braden y tenían cáncer de próstata como diagnóstico primario (20%), seguido del cuello uterino (15%). Conclusión: La presencia de lesión medular y el uso de pañal desechable mostró una fuerte correlación con el desarrollo de lesión por presión, que son los factores predictivos identificados en este estudio. Conocer el perfil de estos pacientes ayuda en la elaboración y sistematización de los procedimientos de enfermería, buscando una mejor calidad y seguridad en la atención. (AU)

Emerging Approaches for Mitigating Biofilm-Formation-Associated Infections in Farm, Wild, and Companion Animals.

The importance of addressing the problem of biofilms in farm, wild, and companion animals lies in their pervasive impact on animal health and welfare. Biofilms, as resilient communities of microorganisms, pose a persistent challenge in causing infections and complicating treatment strategies. Recognizing and understanding the importance of mitigating biofilm formation is critical to ensuring the welfare of animals in a variety of settings, from farms to the wild and companion animals. Effectively addressing this issue not only improves the overall health of individual animals, but also contributes to the broader goals of sustainable agriculture, wildlife conservation, and responsible pet ownership. This review examines the current understanding of biofilm formation in animal diseases and elucidates the complex processes involved. Recognizing the limitations of traditional antibiotic treatments, mechanisms of resistance associated with biofilms are explored. The focus is on alternative therapeutic strategies to control biofilm, with illuminating case studies providing valuable context and practical insights. In conclusion, the review highlights the importance of exploring emerging approaches to mitigate biofilm formation in animals. It consolidates existing knowledge, highlights gaps in understanding, and encourages further research to address this critical facet of animal health. The comprehensive perspective provided by this review serves as a foundation for future investigations and interventions to improve the management of biofilm-associated infections in diverse animal populations.

Epidemiological and Clinical Aspects of Cutaneous and Mucosal Leishmaniases in Portugal: Retrospective Analysis of Cases Diagnosed in Public Hospitals and Reported in the Literature between 2010 and 2020.

Leishmania infantum, a zoonotic vector-born parasite, is endemic in the Mediterranean region, presenting mostly as visceral (VL), but also as cutaneous (CL) and mucosal leishmaniasis (ML). This study aimed to describe the epidemiological and clinical aspects of the CL and ML cases diagnosed in mainland Portugal between 2010 and 2020. Collaboration was requested from every hospital of the Portuguese National Health System. Cases were screened through a search of diagnostic discharge codes or positive laboratory results for Leishmania infection. Simultaneously, a comprehensive literature search was performed. Descriptive statistics and hypothesis testing were performed using IBM® SPSS® Statistics. A total of 43 CL and 7 ML cases were identified, with a predominance of autochthonous cases (86%). In CL, immunosuppressed individuals constituted a significant proportion of patients (48%), and in this group, disseminated CL (22%) and simultaneous VL (54%) were common. In autochthonous cases, lesions, mostly papules/nodules (62%), were frequently observed on the head (48%). The approach to treatment was very heterogeneous. ML cases were all autochthonous, were diagnosed primarily in older immunosuppressed individuals, and were generally treated with liposomal amphotericin B. The findings suggest a need for enhanced surveillance and reporting, clinical awareness, and diagnostic capacity of these forms of leishmaniasis to mitigate underdiagnosis and improve patient outcomes. A holistic One Health approach is advocated to address the multifaceted challenges posed by leishmaniases in Portugal and beyond.

Potencialidades e fragilidades da Sistematização da Assistência de Enfermagem no período puerperal / Strengths and weaknesses of the Systematization of Nursing Care in the postpartum period / Fortalezas y deblilidades de la Sistematización de la Atención de Enfermería en el posparto

Objetivo: identificar potencialidades e fragilidades da Sistematização da Assistência de Enfermagem no período puerperal. Método: estudo descritivo exploratório qualitativo desenvolvido entre agosto e dezembro de 2021 com 17 enfermeiros que atuam em equipes de Estratégia de Saúde da Família em um município do Rio Grande do Sul. Os dados foram coletados por meio de entrevista semiestruturada e submetidos à análise de conteúdo.Resultados: as potencialidades da Sistematização da Assistência de Enfermagemestão relacionadas à satisfação, organização do processo de trabalho e melhoria da qualidade do cuidado. E, as fragilidades estão associadas à falta de recursos humanos e de tempo, acúmulo de funções e pouco conhecimento sobre a temática. Conclusões: os enfermeiros reconhecem a importância da Sistematização da Assistência de Enfermagem, mas ainda é necessário qualificar o processo de enfermagem às mulheres no puerpério

Objective:to identify potentialities and weaknesses of the Systematization of Nursing Care in the postpartum period. Method:qualitative exploratory descriptive study developed between August and December 2021 with 17 nurses who work in Family Health Strategy teams in a city in Rio Grande do Sul. Data were collected through semi-structured interviews and subjected to content analysis. Results:the potential of Nursing Care Systematization is related to satisfaction, organization of the work process and improvement in the quality of care. And, the weaknesses are associated with the lack of human resources and time, accumulation of functions and little knowledge on the subject. Conclusions:nurses recognize the importance of Systematizing Nursing Care, but it is still necessary to qualify the nursing process for women in the postpartum period.

Objetivo: identificar potencialidades y debilidades de la Sistematización de la Atención de Enfermería en el posparto. Método:estudio descriptivo exploratorio cualitativo desarrollado entre agosto y diciembre de 2021 con 17 enfermeros que actúan en equipos de la Estrategia de Salud de la Familia en una ciudad de Rio Grande do Sul. Los datos fueron recolectados a través de entrevistas semiestructuradas y sometidos a análisis de contenido. Resultados:el potencial de la Sistematización de la Atención de Enfermería está relacionado con la satisfacción, la organización del proceso de trabajo y la mejora de la calidad de la atención. Y, las debilidades están asociadas a la falta de recursos humanos y de tiempo, acumulación de funciones y poco conocimiento sobre el tema. Conclusiones:los enfermeros reconocen la importancia de Sistematizar la Atención de Enfermería, pero aún es necesario calificar el proceso de enfermería para las mujeres en el posparto.

Unsupervised analysis of whole transcriptome data from human pluripotent stem cells cardiac differentiation.

The main objective of the present work was to highlight differences and similarities in gene expression patterns between different pluripotent stem cell cardiac differentiation protocols, using a workflow based on unsupervised machine learning algorithms to analyse the transcriptome of cells cultured as a 2D monolayer or as 3D aggregates. This unsupervised approach effectively allowed to portray the transcriptomic changes that occurred throughout the differentiation processes, with a visual representation of the entire transcriptome. The results allowed to corroborate previously reported data and also to unveil new gene expression patterns. In particular, it was possible to identify a correlation between low cardiomyocyte differentiation efficiencies and the early expression of a set of non-mesodermal genes, which can be further explored as predictive markers of differentiation efficiency. The workflow here developed can also be applied to analyse other stem cell differentiation transcriptomic datasets, envisaging future clinical implementation of cellular therapies.

Ultrasound biomicroscopy in the quantification of brain perfusion parameters of a rat stroke model: Analysis of contrast agent bolus kinetic dynamics.

BACKGROUND: Ischemic stroke represents a significant global health concern, necessitating thorough investigations and the utilization of stroke animal models to explore novel treatment modalities and diagnostic imaging techniques. NEW METHOD: Ultrasound biomicroscopy (BMU), operating at a center frequency of 21 MHz, along with ultrasound contrast agents (UCAs), was used to quantify microcirculation cerebral blood flow in a rat model of ischemic stroke. The microcirculation parameters were derived from time intensity curve (TIC) plots obtained based on UCA-bolus kinetics. RESULTS: Semiquantitative perfusion-related parameters were assessed. The TIC curves showed differences in amplitude when compared intra-animal between the left and right sides, and three situations were observed: normal perfusion, hypoperfusion, and nonperfusion. ROC analysis of delays between the left and right time intensity peak (TIP) for regions of interest (ROIs) in the control and stroke-hypoperfusion groups revealed an optimal cutpoint of 0.39 s to indicate when hypoperfusion is occurring in rats, with a sensitivity of 93.33 % and a specificity of 80 %. COMPARISON WITH EXISTING METHOD(S): Ultrasound perfusion imaging through the temporal bone window has been clinically applied to stroke patients using a UCA bolus for TIC analysis. TIC parameters were correlated with MRI- and CT-based measurements. CONCLUSIONS: This investigation quantified cerebral blood flow in a rat model of ischemic stroke by measuring microcirculation parameters. The study demonstrated the efficacy of this approach as a valuable tool for conducting preclinical studies.

Adolescentes a vivenciar o cancro parental: um estudo qualitativo / Adolescents experiencing parental cancer: a qualitative study / Adolescentes que experimentan cáncer en sus padres: un estudio cualitativo

RESUMO Objetivo: analisar necessidades dos adolescentes vivenciando o cancro parental. Método: estudo qualitativo exploratório e descritivo. Coleta de dados foi realizada através de entrevistas semiestruturadas em 2021. Amostra de conveniência foi composta por adolescentes dos 14-19 anos, com pais com doença oncológica na fase de tratamento, acompanhados em um hospital oncológico português. Tratamento de dados foi realizado com recurso à análise de conteúdo. Resultados: da análise das 13 entrevistas, emergiram cinco domínios: Confronto com a doença; Conhecimento sobre a doença; Experiências anteriores com a doença oncológica; Vivência do cancro parental; e Alterações somáticas. Os resultados evidenciam que os adolescentes vivenciando cancro parental apresentam necessidades emocionais, educacionais e psicossociais. Conclusão: este estudo permitiu conhecer as necessidades dos adolescentes, compreendendo as dificuldades sentidas e os desafios que a experiência acarreta. Os resultados permitirão sustentar o desenho de um programa de intervenção de enfermagem para a díade que vivencia o cancro parental.

ABSTRACT Objective: to analyze the needs of adolescents experiencing parental cancer. Method: an exploratory and descriptive qualitative study. Data collection was carried out through semi-structured interviews in 2021. The convenience sample was composed of adolescents aged 14-19, with parents with oncological disease in the treatment phase, followed in a Portuguese oncology hospital. Data processing was carried out using content analysis. Results: from the analysis of the 13 interviews, five domains emerged: Coping with the disease; Knowledge about the disease; Previous experiences with the oncological disease; Experiencing parental cancer; and Somatic changes. The results show that adolescents experiencing parental cancer have emotional, educational and psychosocial needs. Conclusion: this study allowed us to understand adolescents' needs, understanding the difficulties experienced and the challenges that the experience entails. The results will support the design of a nursing intervention program for the dyad experiencing parental cancer.

RESUMEN Objetivo: analizar las necesidades de los adolescentes que experimentan cáncer en sus padres. Método: estudio cualitativo exploratorio y descriptivo. La recolección de datos se realizó mediante entrevistas semiestructuradas en 2021. La muestra por conveniencia estuvo compuesta por adolescentes de 14 a 19 años, con padres con enfermedad oncológica en fase de tratamiento, seguidos en un hospital oncológico portugués. El procesamiento de los datos se realizó mediante análisis de contenido. Resultados: del análisis de las 13 entrevistas surgieron cinco dominios: Enfrentamiento a la enfermedad; Conocimiento sobre la enfermedad; Experiencias previas con la enfermedad oncológica; Experiencia del cáncer de los padres; y Cambios somáticos. Los resultados muestran que los adolescentes que padecen cáncer en sus padres tienen necesidades emocionales, educativas y psicosociales. Conclusión: este estudio permitió comprender las necesidades de los adolescentes, comprendiendo las dificultades vividas y los desafíos que conlleva la experiencia. Los resultados apoyarán el diseño de un programa de intervención de enfermería para la díada que sufre cáncer en sus padres.

Magnetic Chitosan Bionanocomposite Films as a Versatile Platform for Biomedical Hyperthermia.

Responsive magnetic nanomaterials offer significant advantages for innovative therapies, for instance, in cancer treatments that exploit on-demand delivery on alternating magnetic field (AMF) stimulus. In this work, biocompatible magnetic bionanocomposite films are fabricated from chitosan by film casting with incorporation of magnetite nanoparticles (MNPs) produced by facile one pot synthesis. The influence of synthesis conditions and MNP concentration on the films' heating efficiency and heat dissipation are evaluated through spatio-temporal mapping of the surface temperature changes by video-thermography. The cast films have a thickness below 100 µm, and upon exposure to AMF (663 kHz, 12.8 kA m-1 ), induce exceptionally strong heating, reaching a maximum temperature increase of 82 °C within 270 s irradiation. Further, it is demonstrated that the films can serve as substrates that supply heat for multiple hyperthermia scenarios, including: i) non-contact automated heating of cell culture medium, ii) heating of gelatine-based hydrogels of different shapes, and iii) killing of cancerous melanoma cells. The films are versatile components for non-contact stimulus with translational potential in multiple biomedical applications.

Estilos de vida dos estudantes do ensino superior: Contributos para a promoção da saúde / Higher education students' lifestyles: Contributions to health promotion / Estilos de vida de los estudiantes de educación superior: Contribuciones a la promoción de la salud

Resumo Enquadramento: A transição para o ensino superior representa um período de mudança em que o jovem se depara com novas vivências, com implicações no seu estilo de vida. Objetivo: Identificar os domínios dos Estilos de vida dos estudantes do ensino superior que necessitam de intervenção. Metodologia: Estudo descritivo-correlacional, transversal, com uma amostra de 522 estudantes do ensino superior. Questionário de Estilos de Vida Fantástico. Análise estatística em IBM SPSS Statistics 25.0. Resultados: Mediana de idade 22 anos, 78,4% sexo feminino. Dos 522 estudantes, 17,8% excesso peso; 6,3 % obesidade; 14.4% portadores de doença crónica; 35,6% apresentaram Estilos de Vida Muito Bom e 20,1% Estilos de Vida Bom. Destacou-se pela negativa Comportamentos Saúde e Sexual (x-= 8,97/24), Trabalho/Tipo Personalidade (x-= 6,82/12). Há diferenças significativas no score global do questionário (U = 19785,00; p < 0,05) para estudantes as mulheres com melhor Estilo de Vida (x-= 89,16; DP = 12,75); e em função Ano do curso (H = 18,80; p < 0,01). Conclusão: A avaliação dos Estilos de Vida permitiu caraterizar os focos de intervenção e orientar intervenções de promoção da saúde.

Abstract Background: The transition to higher education is a period of change during which students have new experiences that influence their lifestyles. Objective: To identify the domains of higher education students' lifestyles that require intervention. Methodology: This cross-sectional descriptive correlational study was conducted with 522 higher education students. The Fantastic Lifestyle Assessment questionnaire was used, and statistical analysis was conducted using the IBM SPSS Statistics software - version 25.0. Results: The study participants had a median age of 22, and 78.4% were women. Of the 522 students in the sample, 17.8% were overweight and 6.3% were obese, 14.4% were diagnosed with a chronic disease, 35.6% had Very Good lifestyles, and 20.1% had Good lifestyles. The domains Health and sexual behavior (x-= 8.97/24) and Work/type of personality (x-= 6.82/12) obtained negative scores. Significant differences were found in the questionnaire's total score (U =19785.00; p < 0.05) when considering the variables Gender, with female students having better lifestyles (x-= 89.16; SD = 12.75) and Course year (H = 18.80; p < 0.01). Conclusion: Assessing higher education students' lifestyles offers the possibility to describe the intervention foci and guide the health promotion interventions.

Resumen Marco contextual: La transición a la enseñanza superior representa un periodo de cambio en el que los jóvenes se enfrentan a nuevas experiencias con implicaciones para su estilo de vida. Objetivo: Identificar los dominios de los estilos de vida de los estudiantes de enseñanza superior que necesitan intervención. Metodología: Estudio descriptivo-correlacional, transversal, muestra de 522 estudiantes de enseñanza superior. Cuestionario de estilos de vida Fantástico. Análisis estadístico en IBM SPSS Statistics 25.0. Resultados: Mediana de edad de 22 años, el 78,4% del sexo femenino. De los 522 estudiantes, el 17,8% con exceso de peso; el 6,3 % con obesidad; el 14.4% con una enfermedad crónica; el 35,6% presentó estilos de vida muy bueno, y el 20,1% estilos de vida bueno. Salud y comportamento sexual destacó negativamente (x-= 8,97/24), Trabajo/Tipo Personalidad (x-= 6,82/12). Existen diferencias significativas en la puntuación global del cuestionario (U = 19785,00; p < 0,05) para estudantes mujeres con un mejor estilo de vida (x-= 89,16; DP = 12,75) y dependiendo del año del curso (H = 18,80; p < 0,01). Conclusión: La evaluación de estilos de vida permitió caracterizar los focos de intervención y orientar las intervenciones de promoción de la salud.

The experience of parental cancer from the parents' perspective: A qualitative study / La experiencia del cáncer parental desde la perspectiva de los padres: Un estudio cualitativo

INTRODUCTION: Parental cancer is the experience of cancer at an early age in adulthood in people with dependent children, leading to changes in parent-child interaction, family dynamics, the performance of the parental role and socio-economic difficulties. OBJECTIVE: To analyze parents' perspectives who are undergoing cancer treatment to understand the impact this has on their parental role. METHODOLOGY: Qualitative study. Data were collected through semi-structured interviews based on a structured script founded on the Betty Neuman Model. The sample consisted of cancer patients in the treatment phase, with different types of cancer, monitored in a Portuguese cancer hospital, with teenage children aged 14 to 19. Data analysis was carried out using content analysis according to Bardin. The ethical assumptions associated with the study were safeguarded. RESULTS: From the analysis of the 13 interviews carried out, the domain Experience of parental cancer emerged, with two categories "Everything changed in us: the cancer diagnosis" and "Life goes on: repercussions of parental cancer on parent-child interaction" and respective subcategories. CONCLUSION: This study demonstrated the implications of the diagnosis of cancer and the repercussions of parental cancer on parent-child interaction. The results will support the design of a nursing intervention program for the dyad experiencing parental cancer.

INTRODUCCIÓN: El cáncer parental comprende la vivencia del cáncer en edades tempranas de la vida adulta, en personas con hijos dependientes, provocando cambios en la interacción padres-hijos, dinámica familiar, desempeño del rol parental y dificultades socioeconómicas. OBJETIVO: Analizar la perspectiva de los padres sometidos a tratamiento contra el cáncer para comprender el impacto en el rol parental. METODOLOGÍA: Estudio cualitativo. La recolección de datos se realizó a través de entrevistas semiestructuradas, a partir de un guión estructurado, basado en el modelo de Betty Neuman. La muestra estuvo compuesta por pacientes oncológicos en fase de tratamiento, con diferentes tipos de cáncer, seguidos en un hospital oncológico portugués, con hijos adolescentes entre 14 y 19 años. El análisis de datos se realizó mediante análisis de contenido según Bardin. Se salvaguardaron los supuestos éticos inherentes a la realización del estudio. RESULTADOS: Del análisis de las 13 entrevistas realizadas surgió el dominio Experimentar el cáncer de los padres, con dos categorías "Todo ha cambiado en nosotros: el diagnóstico del cáncer" y "La vida continúa: repercusiones del cáncer de los padres en la interacción padres-hijos" y sus respectivas subcategorías. CONCLUSIÓN: Este estudio destacó las implicaciones que existen al diagnosticar una enfermedad oncológica y las repercusiones del cáncer de los padres en la interacción entre padres e hijos. Los resultados apoyarán la construcción del diseño de un programa de intervención de enfermería para la díada que experimenta cáncer parental.

Limited Antineutrophil Cytoplasmic Antibodies (ANCA)-Negative Granulomatosis With Polyangiitis: Successful Response to Rituximab.

Granulomatosis with polyangiitis (GPA), a systemic vasculitis, is commonly characterized by the presence of antineutrophil cytoplasmic antibodies (ANCA). However, a subset of patients with limited disease may exhibit ANCA negativity. In this article, we report the case of a 40-year-old female diagnosed with GPA with intolerance to methotrexate titration and glucocorticoid therapy, leading to the initiation of rituximab treatment. Subsequently, the patient exhibited sustained clinical, laboratory, and radiological improvement. The identification of limited GPA has important therapeutic implications as the effectiveness of the medical treatment in ANCA-negative GPA may differ. Rituximab has emerged as an optimal treatment, irrespective of ANCA status, offering prolonged responses and a favorable tolerance profile in these patients.

Multitemporal Land Use and Cover Analysis Coupled with Climatic Change Scenarios to Protect the Endangered Taxon Asphodelus bento-rainhae subsp. bento-rainhae.

Climate change and land use and land cover (LULC) change are impacting the species' geographic distribution, causing range shifts and reducing suitable habitats. Asphodelus bento-rainhae subsp. bento-rainhae (AbR) is an endangered endemic plant restricted to Serra da Gardunha (Portugal), and knowledge of those changes will help to design conservation measures. MaxEnt was used to model AbR's current distribution and project it into the future, 2050, using the Shared Socioeconomic Pathway SSP3-7. The Portuguese LULC maps from 1951-1980, 1995, 2007, and 2018 were used to assess and quantify LULC changes over time. The results showed that the AbR current predicted distribution matches its actual known distribution, which will not be affected by future predicted climate change. The significant LULC changes were observed during the study periods 1951-1980 to 2018, particularly between 1951-1980 and 1995. Scrubland and Agriculture decreased by 5% and 2.5%, respectively, and Forests increased by 4% in the study area. In the occurrence area, Agriculture increased, and Forests decreased between 1980 and 2018, due to Orchard expansion (34%) and declines in Chestnut (16.9%) and Pine (11%) areas, respectively. The use of species distribution models and the LULC change analysis contributed to understanding current and future species distribution. The LULC changes will have a significant impact on future species distribution. To prevent the extinction of this endemic species in the future, it is crucial to implement conservation measures, namely species monitoring, replantation, and germplasm conservation, in addition to guidelines for habitat conservation.

Programas de supervisión clínica para enfermeras de cuidados intensivos: protocolo de scoping review / Clinical supervision programs for critical care nurses: scoping review protocol

Introducción: La escasez global de enfermeras trajo consigo una mayor dificultad a la hora de contratar y retener a este tipo de profesionales durante la pandemia provocada por la COVID-19. La supervisión clínica en enfermería, por tratarse de un procedimiento sistemático y estructurado para el desarrollo de la práctica clínica en sus diferentes contextos, parece influir en los indicadores de resultados, en la formación continua y en la trayectoria profesional de las enfermeras. Objetivo: 1) Mapear los programas de supervisión clínica para la incorporación de enfermeras en cuidados intensivos. Método de revisión: método de scoping review definido por el Instituto Joanna Briggs a partir de la siguiente pregunta: ¿cuáles son las características de los programas de supervisión clínica para la incorporación de enfermeras en cuidados intensivos? A la hora de definir los criterios de inclusión, se utilizó la estrategia PCC (población, concepto y contexto), además de artículos escritos en portugués, inglés y castellano; estudios primarios de tipo cuantitativo, cualitativo y mixto; estudios secundarios que responden a la pregunta de la investigación; documentos de textos y de opinión, y estudios encontrados en las referencias bibliográficas secundarias. No se utilizan artículos sin el texto completo, fuera del ámbito de la pregunta de partida y cuyo contexto sean los cuidados intensivos pediátricos y neonatales. La búsqueda se realiza en PubMed/ MEDLINE, SCOPUS, LILACS, OpenGrey, Google Scholar, Web of Science y EBSCOhost, y en las bases de datos asociadas. Presentación e interpretación de los resultados: los dados analizados se presentarán de manera esquemática, tabular y narrativa. (AU)

Background: The global shortage of nurses, considered one of the priority areas of intervention by the World Health Organization, has exposed increased difficulties in recruiting and retaining nurses during the COVID-19 pandemic. Clinical supervision in nursing, as a systematized and structured process for the development of clinical practice in different contexts, seems to contribute to outcomes, in continuous training and in the course of nurses’ professional experience. Aim: 1) To map clinical supervision programmes for the integration of nurses in critical care. Review method: Joanna Briggs Institute method for Scoping review, starting from the question: what are the characteristics of clinical supervision programs for the integration of nurses in critical care? In the inclusion criteria definition, the PCC strategy (population, concept and context) was used; articles written in Portuguese, English and Spanish; quantitative, qualitative, mixed type studies, secondary studies which answer the research question, text and opinion documents and studies found in secondary bibliographic references. Articles without full text, outside the scope of the starting question, and whose context is pediatric and neonatal critical care will be excluded. The search will be carried out in PubMed/ MEDLINE, SCOPUS, LILACS, OpenGrey, Google Scholar, Web of Science and EBSCOhost, with associated databases. Presentation and interpretation of results: The analyzed data will be presented in a diagrammatic, tabular and narrative form. (AU)

Swine Colibacillosis: Global Epidemiologic and Antimicrobial Scenario.

Swine pathogenic infection caused by Escherichia coli, known as swine colibacillosis, represents an epidemiological challenge not only for animal husbandry but also for health authorities. To note, virulent E. coli strains might be transmitted, and also cause disease, in humans. In the last decades, diverse successful multidrug-resistant strains have been detected, mainly due to the growing selective pressure of antibiotic use, in which animal practices have played a relevant role. In fact, according to the different features and particular virulence factor combination, there are four different pathotypes of E. coli that can cause illness in swine: enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC) that comprises edema disease E. coli (EDEC) and enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), and extraintestinal pathogenic E. coli (ExPEC). Nevertheless, the most relevant pathotype in a colibacillosis scenario is ETEC, responsible for neonatal and postweaning diarrhea (PWD), in which some ETEC strains present enhanced fitness and pathogenicity. To explore the distribution of pathogenic ETEC in swine farms and their diversity, resistance, and virulence profiles, this review summarizes the most relevant works on these subjects over the past 10 years and discusses the importance of these bacteria as zoonotic agents.

Afetividade e ambiente de trabalho dos enfermeiros: estudo transversal / Afectividad y ambiente de trabajo de enfermeros: estudio transversal / Affectivity, and nurses' work environmente: a cross-sectional study

Objetivo:identificar as relações de afetividade estabelecidas no ambiente de trabalho dos enfermeiros. Método:estudo quantitativo, do tipo analítico, transversalcomposto por 72 Enfermeiros a trabalharem nos serviços de internamento e de urgência. Foram aplicadas as escalas do "Bem-estar Psicológico", "Estilo de Vida Fantástico" e "Ambiente de Trabalho da Prática de Enfermagem". Resultados:a amostra é constituída maioritariamente por enfermeiras (80,6%);solteiras (61,1%);94,4% quase sempre tem alguém com quem conversar sobre assuntos importantes e 88,9% relatamquase sempre dar e receber carinho. Falta de afetoimpacta negativamente na perceção da colaboração entre médicos e enfermeiros, atualização dos planos de cuidados de enfermagem e relevância do diagnóstico de enfermagem. Conclusão:verificou-se um desequilíbrio nas dimensões afetividade e relações interpessoais, as quais influenciam negativamente a qualidade no ambiente de trabalho.

Objective:explore the relationship between affectivityand the nurses' work environment. Method:quantitative, analytical, cross-sectional studycomposed of 72 nurses working in inpatient and emergency services. The scales of "Psychological Well-being", "Fantastic Lifestyle" and "Nursing Practice Work Environment" were applied. Results:the sample consists mostly of nurses (80,6%), single (61,1%), 94,4% almost always have someone to talk to about important matters and 88.9% report almost always giving and receiving affection. Lack of affection negatively impacts the perception of collaboration between doctors and nurses, updating of nursing care plans, and the relevance of the nursing diagnosis. Conclusion:there was an imbalance in the affectivityand interpersonal relationships,which negatively influence the quality of the work environment.

Objetivo:explorar la relación entre la afectividady el ambiente de trabajo de los enfermeros. Método:estudio cuantitativo, analítico, transversalcompuesto por 72 enfermeros que actúan en servicios de hospitalización y urgencias. Se aplicaron las escalas de "Bienestar Psicológico", "Estilo de Vida Fantástico" y "AmbienteLaboral de la Práctica de Enfermería". Resultados:la muestra está compuesta en su mayoría por enfermeras (80,6%), solteras (61,1%), el 94,4% casi siempre tiene con quien hablar de asuntos importantes y el 88,9% refiere casi siempre dar y recibir cariño. La falta de afecto impacta negativamente en la percepción de colaboración entre médicos y enfermeras, la actualización de los planes de atención de enfermería y la pertinencia del diagnóstico de enfermería. Conclusión:hubo un desequilibrio en las dimensiones afectividad y relaciones interpersonales, que influyen negativamente en la calidad del ambiente de trabajo.