Examination of guanosine and xanthosine nucleotides by HPLC and electrochemical detection.

The suitability of electrochemical detection (ECD) following HPLC separation of guanosine, xanthosine and adenosine nucleotides has been evaluated. Separation of five monophosphates of guanosine was achieved by using a reversed-phase column; di- and triphosphates have also been separated from monophosphates. Adenosine compounds are insensitive to ECD.

Photometric and electrochemical detection of purine and pyrimidine compounds in reversed-phase and ion-exchange high-pressure liquid chromatography.

An investigation of the optimal conditions for the determination of selected purine and pyrimidine compounds has been made by studying the separation efficiency of reversed-phase columns and ion-exchange columns and comparing the sensitivity of photometric and electrochemical detectors. The combination of ion-exchange HPLC with electrochemical detection proved very good for the separation of electroactive compounds, under the experimental conditions studied. Both types of column allow detection at the picomole level. In general the electrochemical detector appears to be the more sensitive of the two types.