Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Lipoma/cirurgia , Lipoma , Neoplasias do Colo/cirurgia , Neoplasias do Colo , Doenças do Colo/etiologia , Intussuscepção/etiologia , Lipoma/patologia , Neoplasias do Colo/patologia , Obstrução Intestinal/etiologia , Tomografia Computadorizada por Raios XRESUMO
The relationship between tonoplast-bound ATPase activity and the magnitude of the electrochemical proton gradient has been investigated on tightly sealed vesicles prepared from rubber-tree (Hevea brasiliensis) latex. A variety of methods have been used to modify, either alone or together, the two components of the electrochemical proton gradient (delta mu H+). When the delta pH component was decreased either by titration with (NH4)2SO4 or by addition of protonophores or nigericin in the presence of K+, ATPase activity was stimulated. On the other hand, when the delta psi component was decreased either by addition of lipophilic cations or by addition of valinomycin in the presence of K+, ATPase activity decreased. It is concluded that activity of the tonoplast-bound ATPase is regulated by changes in the electrochemical proton gradient across the tonoplast, so that, once the maximum proton gradient is established across the tonoplast, any perturbation of the equilibrium state should result in the increased rate of ATP hydrolysis as the enzyme attempts to re-establish the initial gradient.
Assuntos
Adenosina Trifosfatases/metabolismo , Látex/metabolismo , Plantas/enzimologia , Trifosfato de Adenosina/farmacologia , Eletroquímica , Concentração de Íons de Hidrogênio , Membranas Intracelulares/enzimologia , Potenciais da Membrana/efeitos dos fármacos , Plantas/efeitos dos fármacos , Prótons , Termodinâmica , Valinomicina/farmacologiaRESUMO
The effect of Cl- and other anions on the tonoplast H+-translocating ATPase (H+-ATPase) from Hevea brasiliensis (rubber tree) latex was investigated. Cl- and other anions stimulated the ATPase activity of tightly sealed vesicles prepared from Hevea tonoplast, with the following decreasing order of effectiveness: Cl- greater than Br- greater than SO4(2-) greater than NO3-. As indicated by the changes of the protonmotive potential difference, anion stimulation of tonoplast H+-ATPase was caused in part by the ability of these anions to dissipate the electrical potential. This interpretation assumes not a channelling of these anions against a membrane potential, negative-inside, but a modification of the permeability of these ions through the tonoplast membrane. In addition, Cl- and the other anions stimulated the ATPase activity solubilized from the tonoplast membrane. Consequently, the tonoplast H+-pumping ATPase can be considered as an anion-stimulated enzyme. These results are discussed in relation to various models described in the literature for the microsomal H+-ATPase systems claimed as tonoplast entities.