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Comp Biochem Physiol B ; 77(4): 749-53, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6734151

RESUMO

The pattern of histone variants from the meal moth Plodia interpunctella was compared to the mouse histone variant pattern. Plodia contains histones which comigrate on two dimensional gels with H3.2, H3.3, H4 and H2A.Z in mouse. Plodia H2A.1 and H2B.1 migrate somewhat differently from the respective mouse histones. Comparison of the iodinated tryptic peptides of H2A.1 and H2A.Z from mouse and Plodia showed that the H2A.Z proteins have two iodinated peptides that comigrate in the two species and three more that are different. The H2A.1 proteins in the two species have one iodinated peptide which comigrates and two more which migrate very close to each other. The histone variants from three developmental stages, larval, pupal and adult of Plodia interpunctella were also identified and compared. The same histone variant pattern is found through all stages of development. It is concluded that histone gene expression does not change during metamorphosis in Plodia .


Assuntos
Histonas/análise , Lepidópteros/análise , Metamorfose Biológica , Mariposas/análise , Animais , Eletroforese , Larva/análise , Leucemia L1210/metabolismo , Camundongos , Proteínas de Neoplasias/análise , Pupa/análise
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