In vivo brain temperature mapping using polymer optical fiber Bragg grating sensors.

Variation of the brain temperature is strongly affected by blood flow, oxygen supply, and neural cell metabolism. Localized monitoring of the brain temperature is one of the most effective ways to correlate brain functions and diseases such as stroke, epilepsy, and mood disorders. While polymer optical fibers (POFs) are considered ideal candidates for temperature sensing in the brain, they have never been used so far in vivo. Here, we developed for the first, to the best of our knowledge, time an implantable probe based on a microstructured polymer optical fiber Bragg grating (FBG) sensor for intracranial brain temperature mapping. The temperature at different depths of the brain (starting from the cerebral cortex) and the correlation between the brain and body core temperature of a rat were recorded with a sensitivity of 33 pm/°C and accuracy <0.2°C. Our in vivo experimental results suggest that the proposed device can achieve real-time and high-resolution local temperature measurement in the brain, as well as being integrated with existing neural interfaces.

Optoelectronic and mechanical properties of microstructured polymer optical fiber neural probes.

Multifunctional optical fiber-based neural interfaces have attracted significant attention for neural stimulation, recording, and photopharmacology towards understanding the central nervous system. In this work, we demonstrate the fabrication, optoelectrical characterization, and mechanical analysis of four types of microstructured polymer optical fiber neural probes using different soft thermoplastic polymers. The developed devices have integrated metallic elements for electrophysiology and microfluidic channels for localized drug delivery, and can be used for optogenetics in the visible spectrum at wavelengths spanning from 450 nm up to 800 nm. Their impedance, measured by electrochemical impedance spectroscopy, was found to be as low as 21 kΩ and 4.7 kΩ at 1kHz when indium and tungsten wires are used as the integrated electrodes, respectively. Uniform on-demand drug delivery can be achieved by the microfluidic channels with a measured delivery rate from 10 up to 1000 nL/min. In addition, we identified the buckling failure threshold (defined as the conditions for successful implantation) as well as the bending stiffness of the fabricated fibers. Using finite element analysis, we calculated the main critical mechanical properties of the developed probes to avoid buckling during implantation and maintain high flexibility of the probe within the tissue. Our results aim to demonstrate the impact of design, fabrication, and characteristics of the materials on the development of polymer fibers as next-generation implants and neural interfaces.

Soft monolithic infrared neural interface for simultaneous neurostimulation and electrophysiology.

Controlling neuronal activity using implantable neural interfaces constitutes an important tool to understand and develop novel strategies against brain diseases. Infrared neurostimulation is a promising alternative to optogenetics for controlling the neuronal circuitry with high spatial resolution. However, bi-directional interfaces capable of simultaneously delivering infrared light and recording electrical signals from the brain with minimal inflammation have not yet been reported. Here, we have developed a soft fibre-based device using high-performance polymers which are >100-fold softer than conventional silica glass used in standard optical fibres. The developed implant is capable of stimulating the brain activity in localized cortical domains by delivering laser pulses in the 2 µm spectral region while recording electrophysiological signals. Action and local field potentials were recorded in vivo from the motor cortex and hippocampus in acute and chronic settings, respectively. Immunohistochemical analysis of the brain tissue indicated insignificant inflammatory response to the infrared pulses while the signal-to-noise ratio of recordings still remained high. Our neural interface constitutes a step forward in expanding infrared neurostimulation as a versatile approach for fundamental research and clinically translatable therapies.

Two octaves spanning photoacoustic microscopy.

In this study, for the first time, a Photoacoustic Microscopy instrument driven by a single optical source operating over a wide spectral range (475-2400 nm), covering slightly more than two octaves is demonstrated. Xenopus laevis tadpoles were imaged in vivo using the whole spectral range of 2000 nm of a supercontinuum optical source, and a novel technique of mapping absorbers is also demonstrated, based on the supposition that only one chromophore contributes to the photoacoustic signal of each individual voxel in the 3D photoacoustic image. By using a narrow spectral window (of 25 nm bandwidth) within the broad spectrum of the supercontinuum source at a time, in vivo hyper-spectral Photoacoustic images of tadpoles are obtained. By post-processing pairs of images obtained using different spectral windows, maps of five endogenous contrast agents (hemoglobin, melanin, collagen, glucose and lipids) are produced.

Adaptive polymer fiber neural device for drug delivery and enlarged illumination angle for neuromodulation.

Objective. Optical fiber devices constitute significant tools for the modulation and interrogation of neuronal circuitry in the mid and deep brain regions. The illuminated brain area during neuromodulation has a direct impact on the spatio-temporal properties of the brain activity and depends solely on the material and geometrical characteristics of the optical fibers. In the present work, we developed two different flexible polymer optical fibers (POFs) with integrated microfluidic channels (MFCs) and an ultra-high numerical aperture (UHNA) for enlarging the illumination angle to achieve efficient neuromodulation.Approach. Three distinct thermoplastic polymers: polysulfone, polycarbonate, and fluorinated ethylene propylene were used to fabricate two step-index UHNA POF neural devices using a scalable thermal drawing process. The POFs were characterized in terms of their illumination map as well as their fluid delivery capability in phantom and adult rat brain slices. Main results.A 100-fold reduced bending stiffness of the proposed fiber devices compared to their commercially available counterparts has been found. The integrated MFCs can controllably deliver dye (trypan blue) on-demand over a wide range of injection rates spanning from 10 nl min-1to 1000 nl min-1. Compared with commercial silica fibers, the proposed UHNA POFs exhibited an increased illumination area by 17% and 21% under 470 and 650 nm wavelength, respectively. In addition, a fluorescent light recording experiment has been conducted to demonstrate the ability of our UHNA POFs to be used as optical waveguides in fiber photometry.Significance. Our results overcome the current technological limitations of fiber implants that have limited illumination area and we suggest that soft neural fiber devices can be developed using different custom designs for illumination, collection, and photometry applications. We anticipate our work to pave the way towards the development of next-generation functional optical fibers for neuroscience.

CO2-based hollow-core fiber Raman laser with high-pulse energy at 1.95 µm.

In this Letter, we present a high-pulse energy (>10µJ) Raman laser at 1946 nm wavelength directly pumped with a 1533 nm custom-made fiber laser. The Raman laser is based on stimulated Raman scattering (SRS) in an 8 m carbon dioxide (CO2)-filled nested anti-resonant hollow-core fiber. The low-energy phonon emission combined with the inherent SRS process along the low-loss fiber allows the generation of high-pulse energy up to 15.4 µJ at atmospheric CO2 pressure. The Raman laser exhibits good long-term stability and low relative intensity noise of less than 4%. We also investigate the pressure-dependent overlap of the Raman laser line with the absorption band of CO2 at the 2 µm spectral range. Our results constitute a novel, to the best of our knowledge, and promising technology towards high-energy 2 µm lasers.

Enhanced birefringence in conventional and hybrid anti-resonant hollow-core fibers.

A hollow-core anti-resonant fiber (HC-ARF) design based on hybrid silica/silicon cladding is proposed for single-polarization, single-mode and high birefringence. We show that by adding silicon layers in a semi-nested HC-ARF, one of the polarization states can be strongly suppressed while simultaneously maintaining low propagation loss for other polarization states, single-mode and high birefiringence. The optimized HC-ARF design exhibits propagation loss, high birefringence, and polarization-extinction ratio of 0.05 dB/m, 0.5 × 10-4, >300 respectively for y-polarization while the loss of x-polarization is >5 dB/m at 1064 nm. The fiber also has low bend-loss and thus can be coiled to a small bend radii of 5 cm having ≈0.06 dB/m bend loss.

Thermally tunable dispersion modulation in a chalcogenide-based hybrid optical fiber.

A hybrid optical fiber comprising metal electrodes, high performance polymers, and a highly nonlinear glass core is presented in this work as a novel, to the best of our knowledge, platform for mid-infrared nonlinear devices. The fiber allows for electrical tuning of the temperature by joule heating using a set of embedded tungsten wires. Unlike temperature tuning by an external heater, this results in a strong modulation, which introduces alternating signs of its dispersion. Enhanced spectral broadening through supercontinuum generation in the mid-infrared due to this modulation is investigated numerically.

Impact of cladding elements on the loss performance of hollow-core anti-resonant fibers.

Understanding the impact of the cladding tube structure on the overall guiding performance is crucial for designing a single-mode, wide-band, and ultra low-loss nested hollow-core anti-resonant fiber (HC-ARF). Here we thoroughly investigate on how the propagation loss is affected by the nested elements when their geometry is realistic (i.e., non-ideal). Interestingly, it was found that the size, rather than the shape, of the nested elements has a dominant role in the final loss performance of the regular nested HC-ARFs. We identify a unique 'V-shape' pattern for suppression of higher-order modes loss by optimizing free design parameters of the HC-ARF. We find that a 5-tube nested HC-ARF has wider transmission window and better single-mode operation than a 6-tube HC-ARF. We show that the propagation loss can be significantly improved by using anisotropic nested anti-resonant tubes elongated in the radial direction. Our simulations indicate that with this novel fiber design, a propagation loss as low as 0.11 dB/km at 1.55 µm can be achieved. Our results provide design insight toward fully exploiting a single-mode, wide-band, and ultra low-loss HC-ARF. In addition, the extraordinary optical properties of the proposed fiber can be beneficial for several applications such as future optical communication system, high energy light transport, extreme non-nonlinear optics and beyond.

Multi-wavelength high-energy gas-filled fiber Raman laser spanning from 1.53 µm to 2.4 µm.

In this work, we present a high-pulse-energy multi-wavelength Raman laser spanning from 1.53 µm up to 2.4 µm by employing the cascaded rotational stimulated Raman scattering effect in a 5 m hydrogen (H2)-filled nested anti-resonant fiber, pumped by a linearly polarized Er/Yb fiber laser with a peak power of ∼13kW and pulse duration of ∼7ns in the C-band. The developed Raman laser has distinct lines at 1683 nm, 1868 nm, 2100 nm, and 2400 nm, with pulse energies as high as 18.25 µJ, 14.4 µJ, 14.1 µJ, and 8.2 µJ, respectively. We demonstrate how the energy in the Raman lines can be controlled by tuning the H2 pressure from 1 bar to 20 bar.

Mid-infrared photoacoustic gas monitoring driven by a gas-filled hollow-core fiber laser.

Development of novel mid-infrared (MIR) lasers could ultimately boost emerging detection technologies towards innovative spectroscopic and imaging solutions. Photoacoustic (PA) modality has been heralded for years as one of the most powerful detection tools enabling high signal-to-noise ratio analysis. Here, we demonstrate a novel, compact and sensitive MIR-PA system for carbon dioxide (CO2) monitoring at its strongest absorption band by combining a gas-filled fiber laser and PA technology. Specifically, the PA signals were excited by a custom-made hydrogen (H2) based MIR Raman fiber laser source with a pulse energy of ⁓ 18 µJ, quantum efficiency of ⁓ 80% and peak power of ⁓ 3.9 kW. A CO2 detection limit of 605 ppbv was attained from the Allan deviation. This work constitutes an alternative method for advanced high-sensitivity gas detection.

Low-noise tunable deep-ultraviolet supercontinuum laser.

The realization of a table-top tunable deep-ultraviolet (UV) laser source with excellent noise properties would significantly benefit the scientific community, particularly within imaging and spectroscopic applications, where source noise has a crucial role. Here we provide a thorough characterization of the pulse-to-pulse relative intensity noise (RIN) of such a deep-UV source based on an argon (Ar)-filled anti-resonant hollow-core (AR HC) fiber. Suitable pump pulses are produced using a compact commercially available laser centered at 1030 nm with a pulse duration of 400 fs, followed by a nonlinear compression stage that generates pulses with 30 fs duration, 24.2 µJ energy at 100 kHz repetition rate and a RIN of < 1%. Pump pulses coupled into the AR HC fiber undergo extreme spectral broadening creating a supercontinuum, leading to efficient energy transfer to a phase-matched resonant dispersive wave (RDW) in the deep-UV spectral region. The center wavelength of the RDW could be tuned between 236 and 377 nm by adjusting the Ar pressure in a 140 mm length of fiber. Under optimal pump conditions the RIN properties were demonstrated to be exceptionally good, with a value as low as 1.9% at ~ 282 nm. The RIN is resolved spectrally for the pump pulses, the generated RDW and the broadband supercontinuum. These results constitute the first broadband RIN characterization of such a deep-UV source and provide a significant step forward towards a stable, compact and tunable laser source for applications in the deep-UV spectral region.

High pulse energy and quantum efficiency mid-infrared gas Raman fiber laser targeting CO2 absorption at 4.2 µm.

In this Letter, we demonstrate a high pulse energy and linearly polarized mid-infrared Raman fiber laser targeting the strongest absorption line of ${\rm CO}_2$CO2 at $\sim{4.2}\;\unicode {x00B5} {\rm m}$∼4.2µm. This laser was generated from a hydrogen (${\rm H}_2$H2)-filled antiresonant hollow-core fiber, pumped by a custom-made 1532.8 nm Er-doped fiber laser delivering 6.9 ns pulses and 11.6 kW peak power. A quantum efficiency as high as 74% was achieved, to yield 17.6 µJ pulse energy at 4.22 µm. Less than 20 bar ${\rm H}_2$H2 pressure was required to maximize the pulse energy since the transient Raman regime was efficiently suppressed by the long pump pulses.

Noise and spectral stability of deep-UV gas-filled fiber-based supercontinuum sources driven by ultrafast mid-IR pulses.

Deep-UV (DUV) supercontinuum (SC) sources based on gas-filled hollow-core fibers constitute perhaps the most viable solution towards ultrafast, compact, and tunable lasers in the UV spectral region, which can even also extend into the mid-infrared (IR). Noise and spectral stability of such broadband sources are key parameters that define their true potential and suitability towards real-world applications. In order to investigate the spectral stability and noise levels in these fiber-based DUV sources, we generate an SC spectrum that extends from 180 nm (through phase-matched dispersive waves - DWs) to 4 µm by pumping an argon-filled hollow-core anti-resonant fiber at a mid-IR wavelength of 2.45 µm. We characterize the long-term stability of the source over several days and the pulse-to-pulse relative intensity noise (RIN) of the DW at 275 nm. The results indicate no sign of spectral degradation over 110 hours, but the RIN of the DW pulses at 275 nm is found to be as high as 33.3%. Numerical simulations were carried out to investigate the spectral distribution of the RIN and the results confirm the experimental measurements and that the poor noise performance is due to the high RIN of the mid-IR pump laser, which was hitherto not considered in numerical modelling of these sources. The results presented herein provide an important step towards an understanding of the noise mechanism underlying such complex light-gas nonlinear interactions and demonstrate the need for pump laser stabilization.

All-fibre supercontinuum laser for in vivo multispectral photoacoustic microscopy of lipids in the extended near-infrared region.

Among the numerous endogenous biological molecules, information on lipids is highly coveted for understanding both aspects of developmental biology and research in fatal chronic diseases. Due to the pronounced absorption features of lipids in the extended near-infrared region (1650-1850 nm), visualisation and identification of lipids become possible using multi-spectral photoacoustic (optoacoustic) microscopy. However, the spectroscopic studies in this spectral region require lasers that can produce high pulse energies over a broad spectral bandwidth to efficiently excite strong photoacoustic signals. The most well-known laser sources capable of satisfying the multi-spectral photoacoustic microscopy requirements (tunability and pulse energy) are tunable nanosecond optical parametric oscillators. However, these lasers have an inherently large footprint, thus preventing their use in compact microscopy systems. Besides, they exhibit low-repetition rates. Here, we demonstrate a compact all-fibre, high pulse energy supercontinuum laser that covers a spectral range from 1440 to 1870 nm with a 7 ns pulse duration and total energy of 18.3 µJ at a repetition rate of 100 kHz. Using the developed high-pulse energy source, we perform multi-spectral photoacoustic microscopy imaging of lipids, both ex vivo on adipose tissue and in vivo to study the development of Xenopus laevis tadpoles, using six different excitation bands over the first overtone transition of C-H vibration bonds (1650-1850 nm).

Multioctave supercontinuum from visible to mid-infrared and bend effects on ultrafast nonlinear dynamics in gas-filled hollow-core fiber.

Broadband supercontinuum generation is numerically investigated in a Xe-filled nested hollow-core antiresonant (HC-AR) fiber pumped at 3 µm with pulses of 100 fs duration and 15 µJ energy. For a 25 cm long fiber, under 7 bar pressure, the supercontinuum spectrum spans multiple octaves from 400 nm to 5000 nm. Furthermore, the influence of bending on ultrafast nonlinear pulse propagation dynamics is investigated for two types of HC-AR fibers (nested and non-nested capillaries). Our results predict similar nonlinear dynamics for both fiber types and a significant reduction of the spectral broadening under tight bending conditions.

Deep-UV to Mid-IR Supercontinuum Generation driven by Mid-IR Ultrashort Pulses in a Gas-filled Hollow-core Fiber.

Supercontinuum (SC) generation based on ultrashort pulse compression constitutes one of the most promising technologies towards ultra-wide bandwidth, high-brightness, and spatially coherent light sources for applications such as spectroscopy and microscopy. Here, multi-octave SC generation in a gas-filled hollow-core antiresonant fiber (HC-ARF) is reported spanning from 200 nm in the deep ultraviolet (DUV) to 4000 nm in the mid-infrared (mid-IR) having an output energy of 5 µJ. This was obtained by pumping at the center wavelength of the first anti-resonant transmission window (2460 nm) with ~100 fs pulses and an injected pulse energy of ~8 µJ. The mechanism behind the extreme spectral broadening relies upon intense soliton-plasma nonlinear dynamics which leads to efficient soliton self-compression and phase-matched dispersive wave (DW) emission in the DUV region. The strongest DW is observed at 275 nm which corresponds to the calculated phase-matching wavelength of the pump. Furthermore, the effect of changing the pump pulse energy and gas pressure on the nonlinear dynamics and their direct impact on SC generation was investigated. This work represents another step towards gas-filled fiber-based coherent sources, which is set to have a major impact on applications spanning from DUV to mid-IR.

Single-mode, low loss hollow-core anti-resonant fiber designs.

In this paper, we numerically investigate various hollow-core anti-resonant (HC-AR) fibers towards low propagation and bend loss with effectively single-mode operation in the telecommunications window. We demonstrate how the propagation loss and higher-order mode modal contents are strongly influenced by the geometrical structure and the number of the anti-resonant cladding tubes. We found that 5-tube nested HC-AR fiber has a wider anti-resonant band, lower loss, and larger higher-order mode extinction ratio than designs with 6 or more anti-resonant tubes. A loss ratio between the higher-order modes and fundamental mode, as high as 12,000, is obtained in a 5-tube nested HC-AR fiber. To the best of our knowledge, this is the largest higher-order mode extinction ratio demonstrated in a hollow-core fiber at 1.55 µm. In addition, we propose a modified 5-tube nested HC-AR fiber, with propagation loss below 1 dB/km from 1330 to 1660 nm. This fiber also has a small bend loss of ~15 dB/km for a bend radius of 1 cm.

Multi-stage generation of extreme ultraviolet dispersive waves by tapering gas-filled hollow-core anti-resonant fibers.

In this work, we numerically investigate an experimentally feasible design of a tapered Ne-filled hollow-core anti-resonant fiber and we report multi-stage generation of dispersive waves (DWs) in the range 90-120 nm, well into the extreme ultraviolet (UV) region. The simulations assume a 800 nm pump pulse with 30 fs 10 µJ pulse energy, launched into a 9 bar Ne-filled fiber with a 34 µm initial core diameter that is then tapered to a 10 µm core diameter. The simulations were performed using a new model that provides a realistic description of both loss and dispersion of the resonant and anti-resonant spectral bands of the fiber, and also importantly includes the material loss of silica in the UV. We show that by first generating solitons that emit DWs in the far-UV region in the pre-taper section, optimization of the following taper structure can allow re-collision with the solitons and further up-conversion of the far-UV DWs to the extreme-UV with energies up to 190 nJ in the 90-120 nm range. This process provides a new way to generate light in the extreme-UV spectral range using relatively low gas pressure.

High-pulse energy supercontinuum laser for high-resolution spectroscopic photoacoustic imaging of lipids in the 1650-1850 nm region.

We propose a cost-effective high-pulse energy supercontinuum (SC) source based on a telecom range diode laser-based amplifier and a few meters of standard single-mode optical fiber, with a pulse energy density as high as ~25 nJ/nm in the 1650-1850 nm regime (factor >3 times higher than any SC source ever used in this wavelength range). We demonstrate how such an SC source combined with a tunable filter allows high-resolution spectroscopic photoacoustic imaging and the spectroscopy of lipids in the first overtone transition band of C-H bonds (1650-1850 nm). We show the successful discrimination of two different lipids (cholesterol and lipid in adipose tissue) and the photoacoustic cross-sectional scan of lipid-rich adipose tissue at three different locations. The proposed high-pulse energy SC laser paves a new direction towards compact, broadband and cost-effective source for spectroscopic photoacoustic imaging.