[Monoamine, GABA, and glutamergic mechanisms of the anterior hypothalamus in anti-aversive effects of sedative and selective drugs in various models of anxiety]. / Issledovanie monoamin-, GAMK- i glutamatergicheskikh mekhanizmov perednego gipotalamusa v antiaversivnykh éffektakh anksiosedativnykh i anksioselektivnykh sredstv na razlichnykh modeliakh trevogi.

The experiments using "illuminated site" and "threatening situation" avoidance tests on rats microinjected with GABA, glutamic acid, monoamines and their agonists and antagonists, as well as anxiosedative and anxioselective agents into the anterior hypothalamus revealed functional ambiguity in the neurochemical profile of this limbic brain formation in the anxiety states of various aversive genesis. Preliminary intraperitoneal injection of the monoamine and GABA antagonists, followed by the local administration of the antianxiety drugs studied, showed that the antiaversive action of chlordiazepoxide, fenibut, and indoter is manifested in both anxiety models via a GABAergic mechanism in the anterior hypothalamus. The anxiolytic effect of campiron is manifested only under negative-stressor zoosocial conditions and is mediated by the serotoninergic systems of this limbic brain formation.

[The neurochemical profile of the caudate nucleus in the anxiolytic action of benzodiazepine and nonbenzodiazepine tranquilizers on different models of anxiety]. / Neirokhimicheskii profil' khvostatogo iadra v anksioliticheskom deistvii benzodiazepinovykh i nebenzodiazepinovykh trankvilizatorov na razlichnykh modeliakh trevogi.

In the experiments on rats in avoidance response to "eliminated area" and "threatening situation" it is found that the microinjection of monoamines and amino-acids of mediator action, adreno- and dophaminemimetics, their antagonists, and blocker of GABAA receptors picrotoxine into dorsal and ventral sections of caudate nucleus elucidates different functional value and neurochemical special features of examined neostriatum specimens in realization of anxiety states of different origin. Local injection of chlordiazepoxide, phenibutum, indoterum, campirone and campironine into aforementioned sections of the caudate nucleus reduces the state of anxiety in "eliminated area" and/or "threatening situation" response tests thus revealing the similarity with well-known effects of GABA and serotonin and substantial difference in the effects of catecholamines and glutamine acid. It is found that anti-anxious effect of benzodiazepine and nonbenzodiazepine anxiolytics can be mediated through participation of neuron matrices of dorsal and ventral sections of the caudate nucleus having functionally ambiguous neurochemical profile.

[Study of monoamino- and amino acidergic mechanisms of the rat caudate nucleus in antiaversive effects of tranquilizers in various anxiety models]. / Issledovanie monoamin- i aminokislotergicheskikh mekhanizmov khvostatogo iadra krys v relizatsii antiaversivnykh éffektov trankvilizatorov na razlichnykh modeliakh trevogi.

Administration of anxio-sedative drugs into the rat caudate nucleus revealed that antiaversive effects of chlordiazepide, phemibut, and indoter only occur under dominating fear motivation, whereas antiaversive effects of campirone and campironine occur under the influence of negative or stressful zoo-social actions and are realised via the GABA- and serotoninergic type of synaptic switching in the dorsal part of the caudate nucleus.

[The neurochemical profile of the septal nucleus accumbens in the anxiolytic action of tranquilizers on different models of anxiety]. / Neirokhimicheskii profil' prilezhashchego iadra peregorodki v anksioliticheskom deistvii trankvilizatorov na razlichnykh modeliakh trevogi.

In experiments on rats with tests for avoidance of an "illuminated area" and a "threatening situation", microinjection into the septal nucleus accumbens of monoamines and GABA, adreno- and dopaminomimetics, and their antagonists demonstrated a different neurochemical profile of this brain structure in anxiety states of different genesis. Local injections of chlordiazepoxide, pnenibut, indoter, campiron, and campironin into the nucleus weakened the alarm in the test for avoidance of an "illuminated area" and/or a "threatening situation", showing a similarity to the effects of GABA and serotonin but not to those of mesaton (phenylephrine hydrochloride) and dopamine. It is concluded that the antialarm effect of benzodiazepine and nonbenzodiazepine anxiolytics may be mediated by switching into action of neuron matrices of the accumbens nucleus with a different neurochemical profile responsible for the operative control of behavior in changed modality of the aversive stimulus.

[Effect of polyethylene oxide 400 on the mitotic regimen of human peripheral blood leukocytes in culture]. / Vliianie poliétilenoksida 400 na mitoticheskii rezhim leikotsitov perifericheskoi krovi cheloveka v kul'ture.

The effect of different concentrations of cryoprotectant polyethylene oxide (PEO-400) on the mitotic activity of human peripheral blood leucocytes was found to decrease the mitotic activity with a simultaneous increase in the metaphase number as the concentrations of PEO-400 grew. These changes were due to the general mechanism of PEO-400 action associated with its dehydrating and osmotic properties.

[Use of cryopreserved bone marrow in oncological patients with depressed hematopoiesis]. / Primenenie kriokonservirovannogo kostnogo mozga onkologicheskim bol'nym s ugneteniem krovetvoreniia.

The effectiveness of allomyelotransplantation has been assessed in 97 oncological patients with different localization of the tumor process. The data indicate that myelotherapy is a valuable adjunct in treatment of depressed hemopoiesis in oncological patients, which allowed the course of radio and chemotherapy to be continued in most of them.

[Proliferative characteristics of human peripheral blood leukocytes cultured with different cryoprotectors]. / Osobennosti proliferatsii leikotsitov perifericheskoi krovi cheloveka pri kul'tivirovanii s razlichnymi krioprotektorami.

The effect of cryoprotectors, both penetrating (glycerol) and nonpenetrating (polyethyleneoxide 400, oxyethylated glycerol) into cells, used in different concentrations in a short-term culture of human peripheral blood was studied in connection with the proliferating activity of leucocytes. A similar pattern of the effect of these substances on the cell growth in culture was observed. Some features of leucocyte proliferation were established such as a decrease of the mitotic index, an increase in the relative number of metaphases and the number of pathological mitoses with the increase in the concentration of the cryoprotectors under study. Some cytopathic changes in the cells were found.

[Growth of cultured blood cells in the presence of the cryoprotector polyethylene oxide 400]. / Osobennosti rosta kletok krovi v kul'ture v prisutstvii krioprotektora poliétilenoksida 400.

The effect of polyethylene oxide 400 (PEO-400) cryoprotector on the mitotic activity of human peripheral blood leukocytes stimulated with PHA was studied in a culture. The cytologic peculiarities of the blood cell growth on addition of PEO-400 prior to cultivation are manifested in the preservation of the transformation capacity and mitosis despite a significant reduction of the mitotic activity in comparison with control cultures.

[Proliferative capability of nuclested rat bone marrow cells following freeze--thawing]. / Proliferativnais sposobnost' iadernykh kletok kostnogo mozga krys posle zamorazhivaniia--ottaivaniia

A study was made of the proliferative capacity of myelokariocytes of the rat bone marrow after freezing and thawing under protection of the oxyetyl derivative of the tetratomic alcohol; experiments were conducted on mouse-rat radiation chimerae. The rat bone marrow cells proved to retain their proliferative capacity.