Assuntos
Hidrolases Anidrido Ácido , Acrilatos , Lepidópteros/enzimologia , Monoéster Fosfórico Hidrolases/metabolismo , Animais , Linhagem Celular , Membrana Celular/enzimologia , Ecdisterona/farmacologia , Ouabaína/farmacologia , Monoéster Fosfórico Hidrolases/antagonistas & inibidores , ATPase Trocadora de Sódio-Potássio/metabolismoRESUMO
Hydroxylation and conjugation were the principal pathways of metabolism of 22,25-dideoxyecdysone in cockroach fat body cultures. The major metabolite isolated and identified was the tetrahydroxy steroid 22-deoxyecdysone; other exdysteroids isolated, in order of decreasing quantities, were 22-deoxy-26-hydroxyecdysone, 22,25-dideoxy-26-hydroxyecdysone, and 22-deoxy-20-hydroxyecdysone. Cockroach fat body from late-instar nymphs appears to lack the mechanism for hydroxylating at C-22. Radioanalyses of the material obtained from enzymic hydrolysis of the conjugate fraction showed 65, 15 and 20% of tetraols, pentaols, and unhydrolyzed conjugates respectively, and no 22,25-dideoxyecdysone. An azasteroid and two nonsteroidal amines that effectively inhibit the activity of 22,25-dideoxyecdysone in the cockroach leg regenerate-fat body culture system enhanced the metabolism of 22,25-dideoxyecdysone, decreased the quantity of the pentaol fraction present, and caused an increase or accumulation of the tetraol and conjugate fractions in the fat body culture system.
RESUMO
Two cell lines were derived from primary cultures of embryonic tissue of the tobacco hornworm, Manduca sexta (L.). The cell lines were maintained on hemolymph-free synthetic insect medium. Cytogenetic and immunoligical identification of the lines were carried out. Techniques for obtaining the line and medium, subculturieng and freezing procedures for long-term storage, and the morphological and growth characteristics are described.
Assuntos
Linhagem Celular , Lepidópteros/citologia , Agregação Celular , Divisão Celular , Núcleo Celular/ultraestrutura , Sobrevivência Celular , Células Cultivadas/ultraestrutura , Meios de Cultura , Grânulos Citoplasmáticos/ultraestrutura , Congelamento , Cariotipagem , Vacúolos/ultraestruturaAssuntos
Baratas/metabolismo , Ecdisona/metabolismo , Animais , Hemolinfa/metabolismo , Técnicas In VitroAssuntos
Células Sanguíneas/efeitos dos fármacos , Hemolinfa/citologia , Insetos/crescimento & desenvolvimento , Intestinos/efeitos dos fármacos , Hormônios de Invertebrado/farmacologia , Animais , Células Epiteliais , Epitélio/efeitos dos fármacos , Intestinos/crescimento & desenvolvimento , Larva/crescimento & desenvolvimento , Metamorfose Biológica , Microscopia de Contraste de Fase , Morfogênese , Plantas Tóxicas , Pupa/crescimento & desenvolvimento , Estimulação Química , NicotianaAssuntos
Técnicas de Cultura , Insetos/crescimento & desenvolvimento , Hormônios de Invertebrado/farmacologia , Animais , Isótopos de Carbono , Colestanos/farmacologia , DNA/biossíntese , Dípteros/crescimento & desenvolvimento , Ecdisona/farmacologia , Morfogênese , Sistemas Neurossecretores/metabolismo , Técnicas de Cultura de Órgãos , RNA/biossíntese , Estimulação Química , Timidina/metabolismo , Uracila/metabolismo , Asas de Animais/efeitos dos fármacosRESUMO
Regenerating epithelial tissue from the cockroach leg forms two cuticular coverings. The first of these consists of a protein-carbohydrate complex that is replaced before molting. The second covering represents the cuticle containing chitin. The deposition of the cuticle can be induced in vitro by adding ecdysterone to the culture medium.
