Retrospective review of eyelid coloboma in snow leopards (Panthera uncia) housed under managed care in North America: 49 cases (2000-2020).

OBJECTIVE: To determine incidence, trends in presentation, associated ocular lesions and other diseases, treatment modalities, and case outcomes of eyelid coloboma cases in snow leopards. ANIMALS: 49 snow leopards with eyelid coloboma living under managed care in North America. METHODS: Medical records were retrospectively searched to identify snow leopards in which eyelid coloboma was diagnosed between January 1, 2000, and December 31, 2020. Data recorded from each animal included signalment, ophthalmic examination information, clinical signs, concurrent health conditions, medical and/or surgical interventions, time to resolution of signs, recurrence of clinical signs, and direct relatives with a history of eyelid coloboma. RESULTS: Ocular clinical signs were present at diagnosis in most cases but not seen in all cases. Corrective procedures were undertaken in 39 cases. Clinical signs were resolved by the total combination of interventions in 84.6% of individuals; however, signs resolved in only 33.3% of cases after a single surgical reconstruction or cryoablation procedure per eye. CLINICAL RELEVANCE: Eyelid coloboma is widespread in the North American snow leopard population. A high percentage (73.5%) have an affected sibling, parent, or grandparent, suggesting a heritable component. Surgical correction resolves or improves clinical signs in most cases, but there is a high rate of postprocedural complications with all procedure types employed. Most complications are minor and manageable, but these can also impact case outcomes. Animals require long-term monitoring, as clinical signs may recur (in some cases, years after initial signs are reported to be resolved), and some animals may require long-term care to manage signs.

Systemic adenovirus infection in Sulawesi tortoises (Indotestudo forsteni) caused by a novel siadenovirus.

A novel siadenovirus was identified in the Sulawesi tortoise (Indotestudo forsteni). A group of 105 Sulawesi tortoises was obtained by the Turtle Survival Alliance. Many of the tortoises were in poor health. Clinical signs included anorexia, lethargy, mucosal ulcerations and palatine erosions of the oral cavity, nasal and ocular discharge, and diarrhea. Initial diagnostic tests included fecal testing for parasites, complete blood count and plasma biochemical analysis, mycoplasma serology, and polymerase chain reaction (PCR) testing for intranuclear coccidia and chelonian herpesvirus. Treatment included administration of antibiotics, antiparasitic medications, parenteral fluids, and nutritional support. Tissue samples from animals that died were submitted for histopathologic evaluation. Histopathologic examination revealed systemic inflammation and necrosis associated with intranuclear inclusions consistent with a systemic viral infection in 35 tortoises out of 50 examined. Fecal testing results and histopathologic findings revealed intestinal and hepatic amoebiasis and nematodiasis in 31 animals. Two of 5 tortoises tested by PCR were positive for Chlamydophila sp. Aeromonas hydrophila and Escherichia coli were cultured from multiple organs of 2 animals. The mycoplasma serology and PCR results for intranuclear coccidia and chelonian herpesvirus were negative. Polymerase chain reaction testing of tissues, plasma, and choanal/cloacal samples from 41 out of 42 tortoises tested were positive for an adenovirus, which was characterized by sequence analysis and molecular phylogenetic inference as a novel adenovirus of the genus Siadenovirus. The present report details the clinical and anatomic pathologic findings associated with systemic infection of Sulawesi tortoises by this novel Siadenovirus, which extends the known reptilian adenoviruses to the chelonians and extends the known genera of reptilian Adenoviridae beyond Atadenovirus to include the genus Siadenovirus.

Fatal hemoprotozoal infections in multiple avian species in a zoological park.

Over a 3-yr span, two juvenile lesser flamingos (Phoeniconaias minor), two green jays (Cyanocorax yncas glaucescens), and two Montezuma oropendolas (Psarocolius montezuma) died peracutely with no premonitory signs at a zoological park in the southern United States. At necropsy, the birds were in excellent body condition. Except for one green jay, the coelomic cavities were filled with a dark serosanguineous fluid. Splenomegaly and hepatomegaly were present. The livers were tan to purple with numerous, randomly distributed red-to-black foci, ranging in size from 1 to 4 mm. The predominant histopathologic finding, except in one green jay, was large protozoal cysts in the hepatic parenchyma. Histologically, the protozoal cysts were restricted to the liver, and none were identified in the skeletal muscle, spleen, or other tissues. Frozen tissue samples harvested at necropsy had a nested polymerase chain reaction assay performed to amplify the mitochondrial cytochrome B gene of the protozoa. The amplified gene sequences were compared with reference cytochrome B gene sequences for avian Plasmodium spp., Haemoproteus spp., and Leucocytozoon spp. The protozoal parasite within the hepatic parenchyma from the Montezuma oropendolas and the lesser flamingos was identified as Haemoproteus spp. Both green jays had Plasmodium spp. isolated from the submitted tissue samples. The peracute nature of the infections precluded any successful medical intervention, making prevention by exclusion the principal means to control hemoprotozoal transmission. There are no reports in the literature documenting identified fatal hemoprotozoal infections in oropendolas, green jays, or lesser flamingos.

Intralesional cisplatin chemotherapy and topical cryotherapy for the control of choanal squamous cell carcinoma in an African penguin (Spheniscus demersus).

A 3.5-yr-old, 2.5-kg female African penguin (Spheniscus demersus) was diagnosed with a choanal squamous cell carcinoma on the basis of biopsy after a history of choanal ulceration and anorexia with weight loss. Therapeutic modalities included the use of intralesional cisplatin followed by localized, topical cryotherapy with liquid nitrogen. The penguin remained free of clinical disease for a period of 9 mo, at which time a second cryotreatment was performed. The patient has remained free of all signs of choanal carcinoma for a period of 13 mo since the second cryotreatment.

Surgical technique for permanent intracoelomic radiotransmitter placement in Anegada iguanas (Cyclura pinguis).

Twenty-four juvenile to subadult Anegada iguanas (Cyclura pinguis), captive-reared in a propagation facility, were designated by a coordinated recovery program for release in the summer of 2003. To facilitate postrelease monitoring, a radiotransmitter device was placed within the coelomic cavity of each animal under general anesthesia before release. The equipment sterilization technique and the method of attachment of the transmitters to the coelomic body wall resulted in functional transmitters and acceptably low rates of mortality associated with the procedure.